Ethylene induced stay-green gene expression regulates drought stress in wheat

761-769Stay-green is an integrated drought adaptation trait characterized by a green leaf phenotype during grain filling under terminal drought. Ethylene is the key hormone for regulating the leaf senescence pathway under natural and stress conditions. The present study was conducted to assess the associative function of ethylene in regulating chlorophyll degrading enzymes viz., chlorophyllase (TaCHLase) and pheophorbide a oxygenase (TaPaO) in wheat (Triticum aestivum L.) under drought stress. Three wheat genotypes (HW 4059, HW 4022 and HW 2078) differing in drought tolerance efficiency were subjected to drought stress for ten days at the reproductive stage. A decline in stay-green traits was found in susceptible genotypes (HW 4059) with yield losses compared to tolerant ones (HW 4022 and HW 2078). The expression level of TaCHLase1 and TaPaO was higher in susceptible genotypes than tolerant ones under drought/osmotic stress. Ethylene upregulated, while ethylene inhibitors downregulated the gene expression. In this study, a novel gene annotated as TaCHLase1 was cloned. The complete cDNA sequence of TaCHLase1 is composed of 1054 bp nucleotides containing an open reading frame of 960 bp encoding 319 amino acids. The encoded protein contained conserved residues such as lipase motif GXSXGG at position 143-148 and putative active site Ser145. Sequence alignment showed TaCHLase1 shares a higher degree of identity with other species. The result suggested that ethylene upregulates the expression of TaCHLase1 gene, inducing chlorophyll degradation. The study further helps in understanding the mechanism of stay-green trait-induced drought tolerance mechanism in wheat

Ethylene induced stay-green gene expression regulates drought stress in wheat

Stay-green is an integrated drought adaptation trait characterized by a green leaf phenotype during grain filling under terminal drought. Ethylene is the key hormone for regulating the leaf senescence pathway under natural and stress conditions. The present study was conducted to assess the associative function of ethylene in regulating chlorophyll degrading enzymes viz., chlorophyllase (TaCHLase) and pheophorbide a oxygenase (TaPaO) in wheat (Triticum aestivum L.) under drought stress. Three wheat genotypes (HW 4059, HW 4022 and HW 2078) differing in drought tolerance efficiency were subjected to drought stress for ten days at the reproductive stage. A decline in stay-green traits was found in susceptible genotypes (HW 4059) with yield losses compared to tolerant ones (HW 4022 and HW 2078). The expression level of TaCHLase1 and TaPaO was higher in susceptible genotypes than tolerant ones under drought/osmotic stress. Ethylene upregulated, while ethylene inhibitors downregulated the gene expression. In this study, a novel gene annotated as TaCHLase1 was cloned. The complete cDNA sequence of TaCHLase1 is composed of 1054 bp nucleotides containing an open reading frame of 960 bp encoding 319 amino acids. The encoded protein contained conserved residues such as lipase motif GXSXGG at position 143-148 and putative active site Ser145. Sequence alignment showed TaCHLase1 shares a higher degree of identity with other species. The result suggested that ethylene upregulates the expression of TaCHLase1 gene, inducing chlorophyll degradation. The study further helps in understanding the mechanism of stay-green trait-induced drought tolerance mechanism in wheat

Reducing the environmental impact of surgery on a global scale: systematic review and co-prioritization with healthcare workers in 132 countries

Abstract Background Healthcare cannot achieve net-zero carbon without addressing operating theatres. The aim of this study was to prioritize feasible interventions to reduce the environmental impact of operating theatres. Methods This study adopted a four-phase Delphi consensus co-prioritization methodology. In phase 1, a systematic review of published interventions and global consultation of perioperative healthcare professionals were used to longlist interventions. In phase 2, iterative thematic analysis consolidated comparable interventions into a shortlist. In phase 3, the shortlist was co-prioritized based on patient and clinician views on acceptability, feasibility, and safety. In phase 4, ranked lists of interventions were presented by their relevance to high-income countries and low–middle-income countries. Results In phase 1, 43 interventions were identified, which had low uptake in practice according to 3042 professionals globally. In phase 2, a shortlist of 15 intervention domains was generated. In phase 3, interventions were deemed acceptable for more than 90 per cent of patients except for reducing general anaesthesia (84 per cent) and re-sterilization of ‘single-use’ consumables (86 per cent). In phase 4, the top three shortlisted interventions for high-income countries were: introducing recycling; reducing use of anaesthetic gases; and appropriate clinical waste processing. In phase 4, the top three shortlisted interventions for low–middle-income countries were: introducing reusable surgical devices; reducing use of consumables; and reducing the use of general anaesthesia. Conclusion This is a step toward environmentally sustainable operating environments with actionable interventions applicable to both high– and low–middle–income countries

Occurrence and Distribution of Lentil Wilt in Major Lentil Growing Regions of Madhya Pradesh, India

In the present investigation, roving survey was conducted during the October to February of 2020–2021 and 2021–22 to acquire information on the natural disease incidence and distribution of lentil wilt in the farmers’ fields in eight different districts of Madhya Pradesh. A pooled mean of disease incidence ranging from 6.62%–20.36% was observed during 2020–22. The district wise scenario of lentil wilt over a period of two consecutive years revealed that maximum average incidence of 18.96% was recorded in Sagar district followed by 17.67% in Khandwa district. However, minimum average wilt incidence of 12.17% was recorded in Mandla district followed by 12.55% in Jabalpur district. Looking to the occurrence of lentil wilt, Sagar, Khandwa, Damoh, Katni and Dindori were identified as hotspot pockets for occurrence of lentil wilt in surveyed districts of Madhya Pradesh. Further, reduction of 26.28% in wilt occurrence was recorded in the farmers’ practice advocated as seed treatment before sowing. Under the seven cropping patterns observed, the minimum wilt incidence of 13.96% was recorded in rice followed by lentil which is the most predominant cropping pattern across the surveyed locations. Further different varieties were screened for occurrence of lentil wilt at Sagar. Among the 14 varieties, maximum incidence of lentil wilt was recorded in PL 5 followed by Shekhar masoor 3. However, two varieties namely JL 1 and L 4076 were found free from wilt incidence in selected hot spot pocket for lentil wilt

Seed Quality Enhancement through Different Priming Treatments in Onion (Allium cepa L.)

In a meticulously executed experiment at the Department of Horticulture, College of Agriculture, Jawaharlal Nehru Krishi Vishwa Vidyalaya, Jabalpur, Madhya Pradesh, during the Rabi season of 2022-23, In the experiment, treatments were arranged in a Factorial Randomized Block Design (FRBD) with two factors concepts. The first factor was mobilized with twelve distinct seed priming treatments i.e.; KNO3 (2%), TiO2 (500ppm), PEG (1MPa), Salicylic acid (50ppm), Pseudomonas (1%), along with water as a control treatment. The second factor assigned to onion varieties i.e., Phule Samarth and B780) Among the findings, the combination of B2A1 (TiO2@ 500 ppm + Phule Samarth) significantly influenced radicle emergence time, while B2A2 (TiO2 @500 ppm + B780) exhibited a noteworthy impact on initial germination counts and final germination counts. Moreover (TiO2 @ 500ppm + B780) recorded distinct effects on plumule length and radicle length. In terms of vigour, variety A2 (B780) demonstrated the highest vigour I with TiO2 @ 500ppm. The results highlighted TiO2 @ 500ppm as the most effective priming agent, with PEG -1Mpa showing less efficacy in promoting seed Vigour II. Additionally, KNO3 @ 2% emerged as a promising agent, and the Phule Samarth variety exhibited a slightly higher mean dry weight compared to B780. Notably, the combination B6A1 (Water + Phule Samarth) resulted in the highest mortality %, while B1A1 (KNO3 @ 2% + Phule Samarth) was particularly associated with intensified pyruvic acid synthesis. Furthermore, the maximum TSS content was observed for B1A1 (KNO3 @ 2% + Phule Samarth), underscoring the intricate interplay between priming and genetic factors

Genotypic Variation Studies in Biomass Partitioning Patterns during Post-flowering Stages under the Late Sown Conditions of Chickpea (Cicer arietinum L.) Germplasm

This study conducted during the 2021-2022 year under field conditions investigates the genotypic variation in biomass partitioning among different chickpea genotypes during post-flowering stages, with a specific emphasis on late sown conditions. The main aim of the study was to elucidate the efficiency of resource allocation toward pod production, measured by the pod harvest index. This research enhances understanding of chickpea performance under delayed sowing conditions by examining genotypic variations in key parameters during critical post-flowering stages, specifically pod filling and seed filling. Biomass partitioning in branches and pods varied considerably during both growth stages, with branches playing a crucial role in pod development during pod filling. The transition from pod filling to seed filling stage resulted in increased biomass partitioning in pods, highlighting their importance as storage organs for seed development. Seed yield exhibited significant variability among genotypes, with some surpassing 2000 kg/ha, while pod harvest index ranged from 35.62% to 70.48%, indicating differences in resource allocation efficiency. Regression analysis showed varying degrees of association between biomass partitioning and pod harvest index, with seed yield and pod harvest index, biomass partitioning in pods exhibiting the highest explanatory power during seed filling. By studying the adaptation of plants towards biphasic and allometric allocation patterns during unfavorable conditions can result in developing the resource use efficient climate resilient varieties. These findings contribute to our understanding of crop productivity under varying environmental conditions and inform strategies for optimizing chickpea yield and resilience

Ethylene induced stay-green gene expression regulates drought stress in wheat

761-769Stay-green is an integrated drought adaptation trait characterized by a green leaf phenotype during grain filling under terminal drought. Ethylene is the key hormone for regulating the leaf senescence pathway under natural and stress conditions. The present study was conducted to assess the associative function of ethylene in regulating chlorophyll degrading enzymes viz., chlorophyllase (TaCHLase) and pheophorbide a oxygenase (TaPaO) in wheat (Triticum aestivum L.) under drought stress. Three wheat genotypes (HW 4059, HW 4022 and HW 2078) differing in drought tolerance efficiency were subjected to drought stress for ten days at the reproductive stage. A decline in stay-green traits was found in susceptible genotypes (HW 4059) with yield losses compared to tolerant ones (HW 4022 and HW 2078). The expression level of TaCHLase1 and TaPaO was higher in susceptible genotypes than tolerant ones under drought/osmotic stress. Ethylene upregulated, while ethylene inhibitors downregulated the gene expression. In this study, a novel gene annotated as TaCHLase1 was cloned. The complete cDNA sequence of TaCHLase1 is composed of 1054 bp nucleotides containing an open reading frame of 960 bp encoding 319 amino acids. The encoded protein contained conserved residues such as lipase motif GXSXGG at position 143-148 and putative active site Ser145. Sequence alignment showed TaCHLase1 shares a higher degree of identity with other species. The result suggested that ethylene upregulates the expression of TaCHLase1 gene, inducing chlorophyll degradation. The study further helps in understanding the mechanism of stay-green trait-induced drought tolerance mechanism in wheat

Sowing Time, Plant Growth Regulators and their Application Scheduling Affect Quality Traits of Wheat (Triticum aestivum L.)

A field experiment was conducted during winter (rabi) seasons of 2020-21, 2021-22 at Research farm, seed technology research center, Department of Plant Physiology, College of Agriculture, JNKVV, Jabalpur, Madhya Pradesh to study the response of sowing time, plant growth regulators and their application scheduling on quality traits of wheat. The experiment consisting of 3 sowing dates (D1-15th November, D2-15th December, D3 - 15th January), 3 Spray scheduling at different stages (A1-Vegetative stage, A2-Anthesis stage, A3-Vegetative + Anthesis stage) and 7 Plant Growth Regulators (H1: Cycocel @1000 ppm, H2: Salicylic acid@400ppm, H3: Salicylic acid@800ppm, KCl@1%, H4: Ascorbic acid@10ppm, H5: Thiourea@400ppm, H6: Water spray and H7: Control) making 63 treatment combinations, replicated thrice in a split plot design. The crop sown at normal sowing time showed significantly positive affect on quality traits of wheat than the late sown crop. Spraying of plant growth regulators salicylic acid@400ppm and 800ppm along with other plant growth regulators at different growth stages have positive effect on seed quality and improve germination %, seedling length, seedling dry weight, seed vigour index I and II of wheat crop

Genetic Evaluation of Recombinant Inbred Lines of Rice Derived from Swarna-Sub1 x AC 20431B for Yield Related Traits

Submergence is the third most important abiotic stress hugely affecting yield and productivity in rice. There are many wild varieties and landraces available which are tolerant to submergence stress. These genotypes can be utilized as donors in different breeding programs. The population derived from such germplasms are subjected to genetic evaluation for yield and yield related attributes for development of lines that are high yielding as well as tolerant to submergence. The present investigation was executed in 120 Recombinant Inbred Lines (RIL) population (F10) derived from Swarna-Sub1 x AC 20431B along with four land races and Swarna-Sub1 to elucidate the association existing between yield and yield attributing characters. Apart from this, Correlation and path analysis was also employed to study direct and indirect effect on grain yield. Observations for agro-morphological traits such as days to 50% flowering, plant height, panicle length, total number of tillers, number of effective tillers per plant, number of filled grains and Spikelet fertility, test weight, grain yield per plant, biomass per plant and harvest index were recorded and analyzed for various variability parameters. Correlation and path coefficient analysis were also carried out. High heritability and genetic advance values were observed for days to 50% flowering, plant height, panicle length, total number of tillers per plant, effective tillers per plant, filled grains per panicle, spikelet fertility, test weight, grain yield and biomass per plant. Among all the characters; filled grains per panicle, biomass and harvest index per plant showed significant correlation with grain yield. Effective number of tillers per plant has the highest positive direct effect on grain yield

Yield optimization, microbial load analysis, and sensory evaluation of mungbean (Vigna radiata L.), lentil (Lens culinaris subsp. culinaris), and Indian mustard (Brassica juncea L.) microgreens grown under greenhouse conditions.

Microgreens have been used for raw consumption and are generally viewed as healthy food. This study aimed to optimize the yield parameters, shelf life, sensory evaluation and characterization of total aerobic bacteria (TAB), yeast and mold (Y&M), Escherichia coli, Salmonella spp., and Listeria spp. incidence in mungbean (Vigna radiata (L.) Wilczek), lentil (Lens culinaris Medikus subsp. culinaris), and Indian mustard (Brassica juncea (L.) Czern & Coss.) microgreens. In mungbean and lentil, seeding-density of three seed/cm2, while in Indian mustard, eight seed/cm2 were recorded as optimum. The optimal time to harvest mungbean, Indian mustard, and lentil microgreens were found as 7th, 8th, and 9th day after sowing, respectively. Interestingly, seed size was found highly correlated with the overall yield in both mungbeans (r2 = .73) and lentils (r2 = .78), whereas no such relationship has been recorded for Indian mustard microgreens. The target pathogenic bacteria such as Salmonella spp. and Listeria spp. were not detected; while TAB, Y&M, Shigella spp., and E. coli were recorded well within the limit to cause any human illness in the studied microgreens. Washing with double distilled water for two minutes has shown some reduction in the overall microbial load of these microgreens. The results provided evidence that microgreens if grown and stored properly, are generally safe for human consumption. This is the first study from India on the safety of mungbean, lentils, and Indian mustard microgreens