13 research outputs found

    Specificity traits consistent with legume-rhizobia coevolution displayed by Ensifer meliloti rhizosphere colonization

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    Rhizobia are α‐ and ß‐proteobacteria that associate with legumes in symbiosis to fix atmospheric nitrogen. The chemical communication between roots and rhizobia begins in the rhizosphere. Using signature‐tagged‐Tn5 mutagenesis (STM) we performed a genome‐wide screening for Ensifer meliloti genes that participate in colonizing the rhizospheres of alfalfa and other legumes. The analysis of ca. 6,000 mutants indicated that genes relevant for rhizosphere colonization account for nearly 2% of the rhizobial genome and that most (ca. 80%) are chromosomally located, pointing to the relevance and ancestral origin of the bacterial ability to colonize plant roots. The identified genes were related to metabolic functions, transcription, signal transduction, and motility/chemotaxis among other categories; with several ORFs of yet‐unknown function. Most remarkably, we identified a subset of genes that impacted more severely the colonization of the roots of alfalfa than of pea. Further analyses using other plant species revealed that such early differential phenotype could be extended to other members of the Trifoliae tribe (Trigonella, Trifolium), but not the Fabeae and Phaseoleae tribes. The results suggest that consolidation of E. meliloti into its current symbiotic state should have occurred in a rhizobacterium that had already been adapted to rhizospheres of the Trifoliae tribe.Fil: Salas, MarĂ­a Eugenia. Consejo Nacional de Investigaciones CientĂ­ficas y TĂ©cnicas. Centro CientĂ­fico TecnolĂłgico Conicet - La Plata. Instituto de BiotecnologĂ­a y BiologĂ­a Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de BiotecnologĂ­a y BiologĂ­a Molecular; ArgentinaFil: Lozano, Mauricio Javier. Consejo Nacional de Investigaciones CientĂ­ficas y TĂ©cnicas. Centro CientĂ­fico TecnolĂłgico Conicet - La Plata. Instituto de BiotecnologĂ­a y BiologĂ­a Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de BiotecnologĂ­a y BiologĂ­a Molecular; ArgentinaFil: LĂłpez, JosĂ© Luis. Consejo Nacional de Investigaciones CientĂ­ficas y TĂ©cnicas. Centro CientĂ­fico TecnolĂłgico Conicet - La Plata. Instituto de BiotecnologĂ­a y BiologĂ­a Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de BiotecnologĂ­a y BiologĂ­a Molecular; ArgentinaFil: Draghi, Walter Omar. Consejo Nacional de Investigaciones CientĂ­ficas y TĂ©cnicas. Centro CientĂ­fico TecnolĂłgico Conicet - La Plata. Instituto de BiotecnologĂ­a y BiologĂ­a Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de BiotecnologĂ­a y BiologĂ­a Molecular; ArgentinaFil: Serrania, Javier. Universitat Phillips; AlemaniaFil: Torres Tejerizo, Gonzalo Arturo. Consejo Nacional de Investigaciones CientĂ­ficas y TĂ©cnicas. Centro CientĂ­fico TecnolĂłgico Conicet - La Plata. Instituto de BiotecnologĂ­a y BiologĂ­a Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de BiotecnologĂ­a y BiologĂ­a Molecular; ArgentinaFil: Albicoro, Francisco Javier. Consejo Nacional de Investigaciones CientĂ­ficas y TĂ©cnicas. Centro CientĂ­fico TecnolĂłgico Conicet - La Plata. Instituto de BiotecnologĂ­a y BiologĂ­a Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de BiotecnologĂ­a y BiologĂ­a Molecular; ArgentinaFil: Nilsson, Juliet Fernanda. Consejo Nacional de Investigaciones CientĂ­ficas y TĂ©cnicas. Centro CientĂ­fico TecnolĂłgico Conicet - La Plata. Instituto de BiotecnologĂ­a y BiologĂ­a Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de BiotecnologĂ­a y BiologĂ­a Molecular; ArgentinaFil: Pistorio, Mariano. Consejo Nacional de Investigaciones CientĂ­ficas y TĂ©cnicas. Centro CientĂ­fico TecnolĂłgico Conicet - La Plata. Instituto de BiotecnologĂ­a y BiologĂ­a Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de BiotecnologĂ­a y BiologĂ­a Molecular; ArgentinaFil: del Papa, Maria Florencia. Consejo Nacional de Investigaciones CientĂ­ficas y TĂ©cnicas. Centro CientĂ­fico TecnolĂłgico Conicet - La Plata. Instituto de BiotecnologĂ­a y BiologĂ­a Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de BiotecnologĂ­a y BiologĂ­a Molecular; ArgentinaFil: Parisi, Gustavo Daniel. Universidad Nacional de Quilmes. Departamento de Ciencia y TecnologĂ­a; Argentina. Consejo Nacional de Investigaciones CientĂ­ficas y TĂ©cnicas; ArgentinaFil: Becker, Anke. Universitat Phillips; AlemaniaFil: Lagares, Antonio. Consejo Nacional de Investigaciones CientĂ­ficas y TĂ©cnicas. Centro CientĂ­fico TecnolĂłgico Conicet - La Plata. Instituto de BiotecnologĂ­a y BiologĂ­a Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de BiotecnologĂ­a y BiologĂ­a Molecular; Argentin

    Power and Energy Management in Integrated Power System

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    Test-retest reliability of dichotic listening test in younger adults with normal hearing

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    Abstract Background Dichotic listening refers to simultaneously stimulating both ears but with two different stimuli. Dichotic listening tasks were used in the audiological test battery to assess auditory processing abilities in children and adults. Studies have reported the dichotic consonant-vowel syllable test’s test-retest reliability in different attentional manipulation conditions. The objectives are to measure the intra- and inter-test reliability of dichotic consonant vowel tests in young, healthy individuals. Methods Sixty participants aged 18–28 with normal hearing sensitivity were recruited for the study. To measure the intra- and inter-test reliability, the dichotic consonant-vowel test was conducted within and between days. The single correct and double correct scores were calculated. Results The analysis of the study showed good test-retest reliability for the single correct and double correct scores of the dichotic consonant-vowel test based on Cronbach’s alpha values and Friedman’s tests. Conclusion This study’s findings indicate that the dichotic test is a stable measure that can be clinically used for evaluation and can be used for research studies
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