1,427 research outputs found
Studying the effects of galactic cosmic radiation on astro- and microbiological model systems
In-depth knowledge regarding the biological effects of the radiation field in space is required for assessing the radiation risks in space. Within the last 50 years, space technology
has provided tools for transporting terrestrial life beyond this protective magnetic field in order
to study in situ responses to selected conditions of space (reviewed in Horneck et al., 2010). From a biological perspective applicable to simple and complex organisms (ranging from biomolecules and microorganisms to humans) various influential physical modifications such as
increased radiation exposure were experienced onboard an orbiting spacecraft in low Earth orbit
(LEO), out- and inside the International Space Station (ISS), orbiting Moon or on the way to
other astrobiological-interesting targets (Mars or icy moons of Saturn or Jupiter). The majority of
experiments on microorganisms in space were performed using Earth-orbiting robotic spacecraft,
e.g., the Russian Foton satellites (FOTON) and the European Retrievable Carrier (EURECA), or
human-tended spacecraft, such as space shuttles and space stations, e.g., MIR and ISS (reviewed
in Nicholson, 2009; Nicholson et al., 2009; Horneck et al., 2010)
Increasing Antibacterial Efficiency of Cu Surfaces by targeted Surface Functionalization via Ultrashort Pulsed Direct Laser Interference Patterning
Copper (Cu) exhibits great potential for application in the design of antimicrobial contact surfaces aiming to reduce pathogenic contamination in public areas as well as clinically critical environments. However, current application perspectives rely purely on the toxic effect of emitted Cu ions, without considering influences on the interaction of pathogenic microorganisms with the surface to enhance antimicrobial efficiency. In this study, it is investigated on how antibacterial properties of Cu surfaces against Escherichia coli can be increased by tailored functionalization of the substrate surface by means of ultrashort pulsed direct laser interference patterning (USPâDLIP). Surface patterns in the scale range of single bacteria cells are fabricated to purposefully increase bacteria/surface contact area, while parallel modification of the surface chemistry allows to involve the aspect of surface wettability into bacterial attachment and the resulting antibacterial effectivity. The results exhibit a delicate interplay between bacterial adhesion and the expression of antibacterial properties, where a reduction of bacterial cell viability of up to 15âfold can be achieved for E. coli on USPâDLIP surfaces in comparison to smooth Cu surfaces. Thereby, it can be shown how the antimicrobial properties of copper surfaces can be additionally enhanced by targeted surface functionalization
Natural microbial populations in a water-based biowaste management system for space life support
AbstractThe reutilization of wastewater is a key issue with regard to long-term space missions and planetary habitation. This study reports the design, test runs and microbiological analyses of a fixed bed biofiltration system which applies pumice grain (16â25 mm grain size, 90Â m2/m3 active surface) as matrix and calcium carbonate as buffer. For activation, the pumice was inoculated with garden soil known to contain a diverse community of microorganisms, thus enabling the filtration system to potentially degrade all kinds of organic matter. Current experiments over 194 days with diluted synthetic urine (7% and 20%) showed that the 7% filter units produced nitrate slowly but steadily (max. 2191 mg NO3âN/day). In the 20% units nitrate production was slower and less stable (max. 1411 mg NO3âN/day). 84% and 76% of the contained nitrogen was converted into nitrate. The low conversion rate is assumed to be due to the high flow rate, which keeps the biofilm on the pumice thin. At the same time the thin biofilm seems to prevent the activity of denitrifiers implicating the existence of a trade off between rate and the amount of nitrogen loss. Microbiological analyses identified a comparatively low number of species (26 in the filter material, 12 in the filtrate) indicating that urine serves as a strongly selective medium and filter units for the degradation of mixed feedstock have to be pre-conditioned on the intended substrates from the beginning
Die VorverstÀrkerentkopplung in der Magnetresonanztomographie
Es wurde ein einfaches Konzept fĂŒr die Entkopplung von Empfangsspulen in der Magnetresonanztomographie beschrieben, das es ermöglicht, die maximale StromunterdrĂŒckung fĂŒr jegliche Art VerstĂ€rker zu finden. Die neue Formulierung verdeutlicht, welche Komponenten beim Entwurf einer solchen Schaltung wichtig sind und unterscheidet sich damit von der ursprĂŒnglichen Darstellung [P. B. Roemer, W. A. Edelstein, C. E. Hayes, S. P. Souza, and O. M. Mueller. The NMR Phased Array. Magnetic Resonance in Medicine, 16(2):192-225, 1990]. Dies wurde in Experiment I nachgewiesen. ZusĂ€tzlich lĂ€sst sich mittels dieser neuen Darstellung ein QualitĂ€tsparameter definieren, der die Stromminimierung charakterisiert und damit vergleichbar macht. Die vereinfachte Darstellung macht auch ein vielfach beobachtetes PhĂ€nomen leicht erklĂ€rbar. Durch die VorverstĂ€rkerentkopplung wird der Strom am Ăbergang A/B minimiert, aber die Spannung wird maximiert. Gerade fĂŒr dicht gepackte Spulenarrays oder benachbarte Spulen, die mit einem "magic overlap" entkoppelt wurden, ist dies problematisch. Diese Methode verringert zwar die magnetische Kopplung, hat aber parasitĂ€re KopplungskapazitĂ€ten zur Folge. Durch die Maximierung der Spannung wird der SignalĂŒbertrag ĂŒber diesen Koppelpfad erhöht. Eine Kombination beider Methoden sollte deshalb vermieden werden. Diese Schlussfolgerung steht im Einklang mit den Beobachtungen anderer Forschungsgruppen [R. F. Lee, R. O. Giaquinto, and C. J. Hardy. Coupling and decoupling theory and its application to the mri phased array. Magnetic Resonance in Medicine, 48(1):203-213, 2002; D. Zanche, Nordmeyer-Massner, Brunner, and Pruessmann. Noise correlation and coupling mechanisms: a comparison of overlapped and non-overlapped surface coils at 3T. In ISMRM, 2008]. AuĂerdem wurde analytisch und experimentell eine besondere Eigenschaft dieser Schaltung nachgewiesen - ihre StabilitĂ€t gegenĂŒber BeladungsĂ€nderung der Spule
UV-radiation-induced formation of DNA bipyrimidine photoproducts in Bacillus subtilis endospores and their repair during germination
The spore photoproduct (SP) is the main DNA lesion after UV-C irradiation, and its repair is crucial for the resistance of spores to UV. The aims of the present study were to assess the formation and repair of bipyrimidine photoproducts in spore DNA of various Bacillus subtilis strains using a sensitive HPLC tandem mass spectrometry assay. Strains deficient in nucleotide excision repair, spore photoproduct lyase, homologous recombination (recA), and with wild-type repair capability were investigated. Additionally, one strain deficient in the formation of major small, acid-soluble spore proteins (SASPs) was tested. In all SASP wild-type strains, UV-C irradiation generated almost exclusively SP (>95 %) but also a few by-photoproducts. In the major SASP-deficient strain, SP and by-photoproducts were generated in equal quantities. The status of the UV-induced bipyrimidine photoproducts was determined at different stages of spore germination. After a germination time of 60 min, >75% of the SP was repaired in wild-type strains and in the SASP-deficient strain, while half of the photoinduced SP was removed in the recA-deficient strain. SP-lyase-deficient spores repaired < 20% of the SP produced. Thus, SP lyase, with respect to nucleotide excision repair, has a remarkable impact on the removal of SP upon spore germination. [Int Microbiol 2007; 10(1):39-46
Molecular response of Deinococcus radiodurans to simulated microgravity explored by proteometabolomic approach
Regarding future space exploration missions and long-term exposure experiments, a detailed
investigation of all factors present in the outer space environment and their effects on organisms of
all life kingdoms is advantageous. Influenced by the multiple factors of outer space, the extremophilic
bacterium Deinococcus radiodurans has been long-termly exposed outside the international Space
Station in frames of the tanpopo orbital mission. the study presented here aims to elucidate molecular
key components in D. radiodurans, which are responsible for recognition and adaptation to simulated
microgravity. D. radiodurans cultures were grown for two days on plates in a fast-rotating 2-D clinostat
to minimize sedimentation, thus simulating reduced gravity conditions. Subsequently, metabolites
and proteins were extracted and measured with mass spectrometry-based techniques. our results
emphasize the importance of certain signal transducer proteins, which showed higher abundances
in cells grown under reduced gravity. these proteins activate a cellular signal cascade, which leads to
differences in gene expressions. Proteins involved in stress response, repair mechanisms and proteins
connected to the extracellular milieu and the cell envelope showed an increased abundance under
simulated microgravity. focusing on the expression of these proteins might present a strategy of cells
to adapt to microgravity conditions
Growth and biofilm formation of Penicillium chrysogenum in simulated microgravity
Penicillium sp. are one of the main fungal genera detected on board the Russian Space Station (MIR) and the International Space Station (ISS), demonstrating its ability to grow on the space stationsÂŽ walls and to maintain growth under microgravity (1-3). As a spore-forming microorganism, Penicillium sp. poses a concern for planetary protection and to human/astronaut health, as its spores, associated with respiratory diseases, can be dispersed through the air (4). Fungal growth on the ISS has shown to promote biodegradation of the spacecraft materials, compromising their integrity. Biofilms are groups of organisms adhered to each other by self-synthesized extracellular polymeric substances, and are ubiquitous in industrial and natural environments (5). It has been reported that Penicillium sp. forms biofilms, which are associated with higher tolerance/resistance to adverse conditions (6). Therefore, biofilm formed on the ISS may have deleterious effects on astronautâs health and/or on ISS materials.
To gain valuable knowledge to control biofilm during long duration spaceflight missions, the NASA-funded project âCharacterization of Biofilm Formation, Growth, and Gene Expression on Different Materials and Environmental Conditions in Microgravityâ is currently being prepared. Pre-flight testing include: defining and optimizing the growth medium and culturing conditions of P. chrysogenum DSM 1075; characterizing the morphological response of P. chrysogenum growth under simulated microgravity; assessing biofilm formation by P. chrysogenum under different conditions.
The study of this fungal strain represents the beginning of a new line of research on board ISS. The knowledge gained can be applicable to a) the safety and maintenance of crewed spacecraft, b) planetary protection, c) mitigation of biofilm-associated illnesses on the crew, as well as on the Earth. Besides, P. chrysogenum is of major medical and historical importance, as it presents the original and present-day industrial source of the antibiotic penicillin, and as an important producer of antifungal proteins and other relevant enzymes
Increasing Antibacterial Efficiency of Cu Surfaces by targeted Surface Functionalization via Ultrashort Pulsed Direct Laser Interference Patterning
Copper (Cu) exhibits great potential for application in the design of antimicrobial contact surfaces aiming to reduce pathogenic contamination in public areas as well as clinically critical environments. However, current application perspectives rely purely on the toxic effect of emitted Cu ions, without considering influences on the interaction of pathogenic microorganisms with the surface to enhance antimicrobial efficiency. In this study, it is investigated on how antibacterial properties of Cu surfaces against Escherichia coli can be increased by tailored functionalization of the substrate surface by means of ultrashort pulsed direct laser interference patterning (USPâDLIP). Surface patterns in the scale range of single bacteria cells are fabricated to purposefully increase bacteria/surface contact area, while parallel modification of the surface chemistry allows to involve the aspect of surface wettability into bacterial attachment and the resulting antibacterial effectivity. The results exhibit a delicate interplay between bacterial adhesion and the expression of antibacterial properties, where a reduction of bacterial cell viability of up to 15âfold can be achieved for E. coli on USPâDLIP surfaces in comparison to smooth Cu surfaces. Thereby, it can be shown how the antimicrobial properties of copper surfaces can be additionally enhanced by targeted surface functionalization
Identification of Differentially Expressed Genes during Bacillus subtilis Spore Outgrowth in High-Salinity Environments Using RNA Sequencing
In its natural habitat, the soil bacterium Bacillus subtilis often has to cope with fluctuating osmolality and nutrient availability. Upon nutrient depletion it can form dormant spores, which can revive to form vegetative cells when nutrients become available again. While the effects of salt stress on spore germination have been analyzed previously, detailed knowledge on the salt stress response during the subsequent outgrowth phase is lacking. In this study, we investigated the changes in gene expression during B. subtilis outgrowth in the presence of 1.2 M NaCl using RNA sequencing. In total, 402 different genes were upregulated and 632 genes were downregulated during 90 min of outgrowth in the presence of salt. The salt stress response of outgrowing spores largely resembled the osmospecific response of vegetative cells exposed to sustained high salinity and included strong upregulation of genes involved in osmoprotectant uptake and compatible solute synthesis. The ÏB-dependent general stress response typically triggered by salt shocks was not induced, whereas the ÏW regulon appears to play an important role for osmoadaptation of outgrowing spores. Furthermore, high salinity induced many changes in the membrane protein and transporter transcriptome. Overall, salt stress seemed to slow down the complex molecular reorganization processes (âripeningâ) of outgrowing spores by exerting detrimental effects on vegetative functions such as amino acid metabolism
Chemical, Thermal, and Radiation Resistance of an Iron Porphyrin: A Model Study of Biosignature Stability
Metal complexes of porphyrins and porphyrin-type compounds are ubiquitous in all three domains of life, with hemes and chlorophylls being the best-known examples. Their diagenetic transformation products are found as geoporphyrins, in which the characteristic porphyrin core structure is retained and which can be up to 1.1 billion years old. Because of this, and their relative ease of detection, metalloporphyrins appear attractive as chemical biosignatures in the search for extraterrestrial life. In this study, we investigated the stability of solid chlorido(2,3,7,8,12,13,17,18-octaethylporphyrinato)iron(III) [FeCl(oep)], which served as a model for heme-like molecules and iron geoporphyrins. [FeCl(oep)] was exposed to a variety of astrobiologically relevant extreme conditions, namely: aqueous acids and bases, oxidants, heat, and radiation. Key results are: (1) the [Fe(oep)]âș core is stable over the pH range 0.0â13.5 even at 80°C; (2) the oxidizing power follows the order ClOâ» > HâOâ > ClOââ» > HNOâ > ClOââ»; (3) in an inert atmosphere, the iron porphyrin is thermally stable to near 250°C; (4) at high temperatures, carbon dioxide gas is not inert but acts as an oxidant, forming carbon monoxide; (5) a decomposition layer is formed on ultraviolet irradiation and protects the [FeCl(oep)] underneath; (6) an NaCl/NaHCOâ salt mixture has a protective effect against X-rays; and (7) no such effect is observed when [FeCl(oep)] is exposed to iron ion particle radiation. The relevance to potential iron porphyrin biosignatures on Mars, Europa, and Enceladus is discussed
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