Hybrids between Pseudoplatystoma corruscans and P. reticulatum (Siluriformes: Pimelodidae) previously reported in the Upper Paraná River are likely escapes from aquaculture farms: evidence from microsatellite markers

ABSTRACT The production of hybrids of the 'pintado', Pseudoplatystoma corruscans (Spix & Agassiz, 1829) and 'cachara', Pseudoplatystoma reticulatum (Eigenmann & Eigenmann, 1889) in captivity has generated many concerns about the possibility of introduction of farmed hybrids into natural environments. In the last decade, hybrids between these species, known as 'pintachara' or 'cachapinta', were reported from different regions of the Upper Paraná River basin. Prospection of these hybrids is important in order to orient conservation programs for the species involved. Knowledge of the presence of these hybrids will direct conservation strategies towards prevention and/or mitigation of the effects of cross breeding in natural populations of P. corruscans (the native species of the genus) and farmed hybrids. In this study, surveyed the larval population using molecular tools to detect the presence and assess the origin (natural hybridization or escapes from fish farms) of hybrids in natural water bodies. Nine microsatellite markers were used to detect signals of hybridization and introgression of P. reticulatum in larvae and adults of P. corruscans in Upper Paraná River basin, between Itaipu Dam and Porto Primavera Dam. The specimens were sampled in the Upper Paraná channel and in tributaries where hybrids had been detected in the past, during two reproductive seasons. Despite of that, no sign of hybridization and introgression was found in the 171 larvae and 75 adults sampled, suggesting that the specimens detected in previous studies had originated from escapes of aquaculture farms

Los procedimientos dentales postnatales afectan la ganancia de peso y el bienestar de los lechones

Objective. We carried out this study to evaluate weight gain (WG), mortality, blood serum proteins (BP), facial lesions of littermates submitted to different teeth procedures. Material and Methods. The experiment was performed in a commercial breeding farm. Were used 15 sows, allotted into three groups: control, where piglets’ teeth were kept intact (IT); teeth clipping (CT); and teeth grinding using an electric grinder (GT). We evaluated WG, BS (five males/litter), mortality, low viable piglets rates and facial lesions in piglets. Additionally, we evaluated sows’ backfat thickness (P2) and teat lesion score. BP data, lesion score and WG were assessed individually. For WG, the initial weight and the litter size were used as covariates. Regarding other variables, we used the average of the litter. When there were differences, the means were compared using Duncan test (p<0.05). Results. In the first week, GT piglets presented higher WG. In the second week, CT presented worst WG. In the first two weeks as in the total period, CT piglets presented worse WG than the GT. Mortality and low viable piglets rates were not influenced. After the fourth day, CT and GT treatment reduced facial lesions. There was no effect on BP. Treatments did not influence P2 and teat lesion score. Conclusions. Treatments did not have influence on mortality, low viable rates, BP of the piglets and P2 and teat lesions score. CT treatment decreased gain and IT increased face lesions score.Objetivo. Este estudio fue realizado para evaluar la ganancia de peso, mortalidad, proteínas plasmáticas (BP), lesiones faciales en los lechones y en las cerdas, grasa dorsal (P2) y lesiones en los pezones en lechigadas sometidas a diferentes manejos dentales. Materiales y métodos. La investigación fue conducida en una granja comercial de cría. Fueron utilizadas quince cerdas, divididas en tres grupos: control, en el cual los dientes de los lechones permanecieron intactos; descolmille con alicate; descolmille con limadora eléctrica. Nosotros evaluamos WG, BP (cinco machos/lechigada), mortalidad, lechones de baja viabilidad y lesiones faciales en los lechones. En las cerdas, nosotros evaluamos P2 y la escala de lesiones en los pezones. Los datos de BP, escala de lesiones y WG fueron evaluados individualmente. Para el WG, el peso inicial y el tamaño de la lechigada fueron considerados. Acerca de las otras variables, nosotros usamos el peso promedio de la lechigada. Cuando hubo diferencias, las medias fueron comparadas usando la prueba de Duncan (p<0.05). Resultados. En la primera semana, los lechones del GT presentaron mayor WG. En la segunda semana el CT presentó la peor WG. En las dos primeras semanas así como en el período total, los lechones del CT presentaron peor WG de que los del GT. La mortalidad y los lechones de baja viabilidad no fueron influenciados. Después del cuarto día, el CT y el GT redujeron las lesiones faciales. No hubo efectos en BP. Los tratamientos no influenciaron P2 y las lesiones en los pezones. Conclusiones. Los tratamientos no influenciaron en la mortalidad, lechones de baja viabilidad, BP de los lechones y en la escala de lesiones en los pezones de las cerdas. CT redujo la ganancia y IT aumentó las lesiones faciales

Proceedings of the 13th annual conference of INEBRIA

CITATION: Watson, R., et al. 2016. Proceedings of the 13th annual conference of INEBRIA. Addiction Science & Clinical Practice, 11:13, doi:10.1186/s13722-016-0062-9.The original publication is available at https://ascpjournal.biomedcentral.comENGLISH SUMMARY : Meeting abstracts.https://ascpjournal.biomedcentral.com/articles/10.1186/s13722-016-0062-9Publisher's versio

Florid cemento-osseous dysplasia: a contraindication to orthodontic treatment in compromised areas

ABSTRACT Florid cemento-osseous dysplasia is a sclerosing disease that affects the mandible, especially the alveolar process, and that is, in most cases, bilateral; however, in some cases it affects up to three or even four quadrants. During the disease, normal bone is replaced with a thinly formed, irregularly distributed tissue peppered with radiolucent areas of soft tissue. Newly formed bone does not seem to invade periodontal space, but, in several images, it is confused with the roots, without, however, compromising pulp vitality or tooth position in the dental arch. There is no replacement resorption, not even when the images suggest dentoalveolar ankylosis. Orthodontists should make an accurate diagnosis when planning treatments, as this disease, when fully established, is one of the extremely rare situations in which orthodontic treatment is contraindicated. This contraindication is due to: (a) procedures such as the installment of mini-implants and mini-plaques, surgical maneuvers to apply traction to unerupted teeth and extractions should be avoided to prevent contamination of the affected bone with bacteria from the oral microbiota; and (b) tooth movement in the areas affected is practically impossible because of bone disorganization in the alveolar process, characterized by high bone density and the resulting cotton-wool appearance. Densely mineralized and disorganized bone is unable to remodel or develop in an organized way in the periodontal ligaments and the alveolar process. Organized bone remodeling is a fundamental phenomenon for tooth movement

Postnatal teeth procedures affect the weight gain and welfare of piglets

Objective. We carried out this study to evaluate weight gain (WG), mortality, blood serum proteins (BP), facial lesions of littermates submitted to different teeth procedures. Material and Methods. The experiment was performed in a commercial breeding farm. Were used 15 sows, allotted into three groups: control, where piglets’ teeth were kept intact (IT); teeth clipping (CT); and teeth grinding using an electric grinder (GT). We evaluated WG, BS (five males/litter), mortality, low viable piglets rates and facial lesions in piglets. Additionally, we evaluated sows’ backfat thickness (P2) and teat lesion score. BP data, lesion score and WG were assessed individually. For WG, the initial weight and the litter size were used as covariates. Regarding other variables, we used the average of the litter. When there were differences, the means were compared using Duncan test (p<0.05). Results. In the first week, GT piglets presented higher WG. In the second week, CT presented worst WG. In the first two weeks as in the total period, CT piglets presented worse WG than the GT. Mortality and low viable piglets rates were not influenced. After the fourth day, CT and GT treatment reduced facial lesions. There was no effect on BP. Treatments did not influence P2 and teat lesion score. Conclusions. Treatments did not have influence on mortality, low viable rates, BP of the piglets and P2 and teat lesions score. CT treatment decreased weight gain and IT increased face lesions score.Objetivo. Este estudio fue realizado para evaluar la ganancia de peso, mortalidad, proteínas plasmáticas (BP), lesiones faciales en los lechones y en las cerdas, grasa dorsal (P2) y lesiones en los pezones en lechigadas sometidas a diferentes manejos dentales. Materiales y métodos. La investigación fue conducida en una granja comercial de cría. Fueron utilizadas quince cerdas, divididas en tres grupos: control, en el cual los dientes de los lechones permanecieron intactos; descolmille con alicate; descolmille con limadora eléctrica. Nosotros evaluamos WG, BP (cinco machos/lechigada), mortalidad, lechones de baja viabilidad y lesiones faciales en los lechones. En las cerdas, nosotros evaluamos P2 y la escala de lesiones en los pezones. Los datos de BP, escala de lesiones y WG fueron evaluados individualmente. Para el WG, el peso inicial y el tamaño de la lechigada fueron considerados. Acerca de las otras variables, nosotros usamos el peso promedio de la lechigada. Cuando hubo diferencias, las medias fueron comparadas usando la prueba de Duncan (p<0.05). Resultados. En la primera semana, los lechones del GT presentaron mayor WG. En la segunda semana el CT presentó la peor WG. En las dos primeras semanas así como en el período total, los lechones del CT presentaron peor WG de que los del GT. La mortalidad y los lechones de baja viabilidad no fueron influenciados. Después del cuarto día, el CT y el GT redujeron las lesiones faciales. No hubo efectos en BP. Los tratamientos no influenciaron P2 y las lesiones en los pezones. Conclusiones. Los tratamientos no influenciaron en la mortalidad, lechones de baja viabilidad, BP de los lechones y en la escala de lesiones en los pezones de las cerdas. CT redujo la ganancia de peso y IT aumentó las lesiones faciales

Methionine-induced hyperhomocysteinemia reverts fibrinolytic pathway activation in a murine model of acute promyelocytic leukemia

AbstractIncreased fibrinolysis is an important component of acute promyelocytic leukemia (APL) bleeding diathesis. APL blasts overexpress annexin II (ANXII), a receptor for tissue plasminogen activator (tPA), and plasminogen, thereby increasing plasmin generation. Previous studies suggested that ANXII plays a pivotal role in APL coagulopathy. ANXII binding to tPA can be inhibited by homocysteine and hyperhomocysteinemia can be induced by L-methionine supplementation. In the present study, we used an APL mouse model to study ANXII function and the effects of hyperhomocysteinemia in vivo. Leukemic cells expressed higher ANXII and tPA plasma levels (11.95 ng/mL in leukemic vs 10.74 ng/mL in wild-type; P = .004). In leukemic mice, administration of L-methionine significantly increased homocysteine levels (49.0 μmol/mL and &lt; 6.0 μmol/mL in the treated and nontreated groups, respectively) and reduced tPA levels to baseline concentrations. The latter were also decreased after infusion of the LCKLSL peptide, a competitor for the ANXII tPA–binding site (11.07 ng/mL; P = .001). We also expressed and purified the p36 component of ANXII in Pichia methanolica. The infusion of p36 in wild-type mice increased tPA and thrombin-antithrombin levels, and the latter was reversed by L-methionine administration. The results of the present study demonstrate the relevance of ANXII in vivo and suggest that methionine-induced hyperhomocysteinemia may reverse hyperfibrinolysis in APL.http://deepblue.lib.umich.edu/bitstream/2027.42/177162/2/2012:07_Jacome RH, et al. Blood.pdfPublished versionDescription of 2012:07_Jacome RH, et al. Blood.pdf : Published versio