Signal processing in slit-scan flow cytometry of cell conjugates

The design and implementation of a real-time signal processing system for slit-scan flow cytometry is described. The system is used to measure the separate scatter and fluorescence peak heights of 2 adherent cells. Preliminary measurements of changes in the membrane potential induced by interactions between natural killer (NK) cells and their target cells are presented

Complexity of Shapiro steps

We demonstrate on the example of the dc+ac driven overdamped Frenkel-Kontorova model that an easily calculable measure of complexity can be used for the examination of Shapiro steps in presence of thermal noise. In real systems, thermal noise causes melting or even disappearance of Shapiro steps, which makes their analysis in the standard way from the response function difficult. Unlike in the conventional approach, here, by calculating the Kolmogorov complexity of certain areas in the response function we were able to detect Shapiro steps, measure their size with desired precision and examine their temperature dependence. The aim of this work is to provide scientists, particularly experimentalists, an unconventional but a practical and easy tool for examination of Shapiro steps in real systems.Comment: 6 pages, 5 figures, References and text edite

Apoptotic changes in animal cell cultures and mechanisms of action of endogenous and exogenous factors

Pročišćeni i izolirani protein iz svinjskog mozga identificiran je kao kiseli fibroblastni faktor rasta. Sirovi ekstrakt svinjskog mozga ima pozitivni učinak na rast stanica u primarnoj kulturi ovarija i uterusa štakora te u CCO i CHO-K1 staničnim linijama. Ispitano je djelovanje 17-etinilestradiola i dietilstilbestrola na rast CCO i CHO-K1 stanica. Oba spoja, pri koncentracijama 1 μg/mL i veće, djeluju citotoksično. 17-Etinilestradiol inducira staničnu smrt procesom apoptoze, dok dietilstilbestrol inducira nekrozu u obje stanične linije.Protein purified and isolated from porcine brain was identified as acidic fibroblast growth factor. Crude brain extract showed positive effect on cell in vitro proliferation in primary culture of rat's ovary and uterus cells as well as in CCO and CHO-K1 cell lines. Effect of 17-ethynylestradiol and diethylstilbestrol was investigated in CCO and CHO-K1 cells. Both compounds, in concentrations 1 μg/ml and higher, showed cytotoxic effect. 17-Ethynylestradiol induced cell death by apoptosis, while diethylstilbestrol induced necrosis in both cell lines

Apoptotic changes in animal cell cultures and mechanisms of action of endogenous and exogenous factors

Pročišćeni i izolirani protein iz svinjskog mozga identificiran je kao kiseli fibroblastni faktor rasta. Sirovi ekstrakt svinjskog mozga ima pozitivni učinak na rast stanica u primarnoj kulturi ovarija i uterusa štakora te u CCO i CHO-K1 staničnim linijama. Ispitano je djelovanje 17-etinilestradiola i dietilstilbestrola na rast CCO i CHO-K1 stanica. Oba spoja, pri koncentracijama 1 μg/mL i veće, djeluju citotoksično. 17-Etinilestradiol inducira staničnu smrt procesom apoptoze, dok dietilstilbestrol inducira nekrozu u obje stanične linije.Protein purified and isolated from porcine brain was identified as acidic fibroblast growth factor. Crude brain extract showed positive effect on cell in vitro proliferation in primary culture of rat's ovary and uterus cells as well as in CCO and CHO-K1 cell lines. Effect of 17-ethynylestradiol and diethylstilbestrol was investigated in CCO and CHO-K1 cells. Both compounds, in concentrations 1 μg/ml and higher, showed cytotoxic effect. 17-Ethynylestradiol induced cell death by apoptosis, while diethylstilbestrol induced necrosis in both cell lines

Shiftworking families: parents' working schedule and sleep patterns of adolescents attending school in two shifts

OBJECTIVE: To explore whether parents' engagement in shift work affects the sleep habits of their adolescent children who attend school in two shifts. METHODS: The data were drawn from an extensive survey of sleep and daytime functioning of adolescents attending school one week in the morning and the other in the afternoon. The participants were 1,386 elementary and high school students (11-18 years old) whose parents were both employed. The data were analyzed using MANOVA, with parents' work schedule, adolescents' gender and type of school as between-subject factors. RESULTS: Parents' working schedule significantly affected the sleep patterns of high school adolescents. When attending school in the morning, adolescents whose parents were both day workers woke up somewhat later than adolescents with one shiftworking parent. In addition, they slept longer than adolescents whose parents were both shift workers. On weekends, adolescents whose parents both worked during the day went to bed earlier than adolescents whose parents were both shiftworkers. They also had smaller bedtime delay on weekends with respect to both morning and afternoon shifts than adolescents for whom one or both parents worked shifts. A significant interaction between parents' working schedule, adolescents' gender and type of school was found for sleep extension on weekends after afternoon shift school. CONCLUSIONS: Parental involvement in shift work has negative effects on the sleep of high school adolescents. It contributes to earlier wake-up time and shorter sleep in a week when adolescents attend school in the morning, as well as to greater bedtime irregularity.<br>OBJETIVO: Investigar se a ocupação de pais com o trabalho em turnos interfere nos hábitos de sono dos filhos adolescentes que freqüentam a escola em dois períodos distintos. MÉTODOS: Os dados foram coletados em uma extensa pesquisa sobre sono e atividades diurnas de adolescentes que freqüentavam a escola no período da manhã e da tarde em semanas alternadas. Participaram do estudo 1.386 estudantes do ensino fundamental e médio (idades de 11-18 anos) cujos pais trabalhavam fora. Os dados foram analisados usando-se MANOVA, sendo o horário de trabalho dos pais, sexo dos adolescentes e tipo de escola os fatores intra-sujeitos. RESULTADOS: O horário de trabalho dos pais apresentou interferência significativa nos padrões de sono de adolescentes do ensino médio. Quando freqüentavam a escola pela manhã, os adolescentes cujos pais trabalhavam no período diurno levantavam-se um pouco mais tarde do que os adolescentes cujo pai ou mãe trabalhava em turnos. Além disso, eles dormiam mais do que os adolescentes cujos pais trabalhavam em turnos. Nos fins de semana, os adolescentes cujos pais trabalhavam no período diurno iam dormir mais cedo do que os adolescentes cujos pais trabalhavam em turnos. Também apresentaram um atraso menor no horário de dormir nos fins de semana depois da escola tanto no período da manhã quanto da tarde comparados aos adolescentes em que o pai ou a mãe ou ambos trabalhavam em turnos. Verificou-se uma interação significativa entre o horário de trabalho dos pais, sexo dos adolescentes e tipo de escola e a duração do sono nos fins de semana depois da escola no período da tarde. CONCLUSÕES:A ocupação dos pais no trabalho em turnos exerce efeitos negativos no sono de adolescentes do ensino médio, contribuindo para um horário de levantar mais cedo e um período de sono mais curto durante a semana quando os adolescentes freqüentam a escola pela manhã, além de uma maior irregularidade na hora de dormir

Introduction

This is a chapter addressed to all researchers interested in entrepreneurship

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