Morfološka i molekularna identifikacija populacija cistolikih nematoda krompira u Srbiji

Quarantine species such as potato cyst nematodes Globodera rostochiensis and G. pallida are present in Serbia since 1999 and 2005, respectively. These nematodes are sibling species and their morphological identification is complex due to their morphometric overlap. The cysts from the localities of Kladnica, Šanac, Gojna Gora and Milatovići were grown on susceptible potato varieties and their morphological differences have been discussed. To avoid ambiguities in species morphological designation a duplex PCR method was chosen for a rapid and accurate species identification. The whole procedure, from DNA extraction to DNA isolation, can be performed in a single day.Karantinske vrste kao što su cistolike nematode krompira Globodera rostochiensis i G. pallida su prisutne u Srbiji od 1999. god. odn. 2005. god. Ove nematode su sestrinske vrste i njihova morfološka identifikacija je složena zbog morfometrijskog preklapanja. Ciste sa lokaliteta Kladnica, Šanac, Gojna Gora i Milatovići su gajene na osetljivim sortama krompira i njihove morfološke razlike su analizirane. Da bi se izbegle nejasnoće u morfološkom određivanju vrsta, izabran je duplex PCR metod za brzu i preciznu specijsku identifikaciju. Celokupna procedura, od ekstrakcije DNK do njene vizualizacije, može biti urađena za jedan dan

The effect of sociodemographic characteristics on the self-evaluation of health among the population in Serbia

This paper analyses the impacts of various demographic and socioeconomic characteristics on the way Serbian people assess their own health. Marital status, education level, and occupation were analysed using the threeway ANOVA statistical method. The results of the statistical analysis show that each of the selected features significantly affects the subjective experience of health conditions. Regarding marital status, unmarried people assessed their health status most favourably. In terms of education, a better perception of health was observed in people with higher levels of education. And considering gender, only marital status had an impact on the self-assessment of health in both sexes. For men, education had an impact, whereas for women, it was the type of occupation. The specificity in perception of one’s own health in the elderly population of Serbia (above 60 years old) is reflected in the fact that occupation was the only factor that influenced the subjective experience of health status. © 2022 Demographic Research Centre. All rights reserved

Novi rezultati o mogućnostima očuvanja sadržaja važnijih sastojaka mleka

Milk yield and content of main milk components are dominantly genetically determined characteristics. This fact must be considered while searching for possibilities for improving milk yield, dry matter, fat and protein. The effect of nutrition on milk yield and content of milk components is very significant. Cows with the genetic potential for production over 6 000 kg of milk in lactation show even more significant impact of this effect. Such genetic potential creates new requirements in nutrition of cows; increased feedstuff quality, inclusion of feedstuff rich in energy and protein addition of minerals, vitamins and other supplements if necessary. Application of proper herd management and working processes on the farm modern technological and technical milking solutions, housing conditions of the cows, also have effect on the milk yield and content of some milk components, mainly dry matter, fat, and partially protein. Usage of cottonseed in nutrition of cows, in amount of 1,5 kg/day contribute to the enhancement of milk production, dry matter, fat and protein in milk. For this reason, especially in nutrition of highyielding cows, use of cottonseed is justified.The limiting factor for use of cottonseed can be disorder of cereal and oilseed meal or cottonseed price parity. Use of minerals with buffer characteristics, based on natural raw minerals, with various and complementary physical and chemical characteristics, has a positive effect on enhancement of milk production, dry matter and fat content in milk. Protein content is slightly changed. Therefore, buffer supplementation especially in stressing conditions of nutrition of highyielding cows, can be fully justified.U radu se analiziraju faktori koji mogu da utiču na promenu sadržaja važnijih sastojaka mleka i prezentiraju najnoviji rezultati istraživanja o mogućnostima njihovog očuvanja. Posebna pažnja posvećena je, pored genetskih, grupi paragenetskih faktora koji se odnose na normative o potrebama grla u hranljivim materijama, kvalitetu i osobinama pojedinih hraniva i dodataka obrocima. Skrenuta je pažnja i na značaj primene odgovarajuće tehnike hranjenja krava. Izneseni su rezultati prema kojima dodatak 1,5 kg zrna pamuka u obrok krava, odnosno 3–4% mineralne predsmeše (na bazi bentonita, organozeolita, sode bikarbone i magnezijum oksida) u superkoncentrat za krave (30% ukupnih proteina) utiče na povećanje mlečne masti za 0,1–0,2 procenat poena uz adekvatno povećanje sadržaja suve materije i neznatno povećanje sadržaja proteina u mleku

Geomorphological and hydrological heritage of Mt. Stara Planina in SE Serbia: From river protection initiative to potential geotouristic destination

Mt. Stara Planina is located in the eastern part of the Republic of Serbia and represents the westernmost part of the large mountain massif of the Balkans. Both endogenous and exogenous forces created interesting geomorphological and hydrological features for geotourism development in this area. This article proposes a preliminary list of geomorphological and hydrological sites and analyses them to reveal which geosite possesses geotourism potential. This research was carried out by applying the modified geosite assessment model. In this article, ten geosites were singled out based on the degree of their attractiveness for geotourism development. The results reveal information about the key fields of improvement for each evaluated geosite, and identify which areas require more attention and better management in the upcoming period for Mt. Stara Planina to become a well-known geotourism destination that would attract a large number of tourists in the future

Origins and genetic legacy of prehistoric dogs

Dogs were the first domestic animal, but little is known about their population history and to what extent it was linked to humans. We sequenced 27 ancient dog genomes and found that all dogs share a common ancestry distinct from present-day wolves, with limited gene flow from wolves since domestication but substantial dog-to-wolf gene flow. By 11,000 years ago, at least five major ancestry lineages had diversified, demonstrating a deep genetic history of dogs during the Paleolithic. Coanalysis with human genomes reveals aspects of dog population history that mirror humans, including Levant-related ancestry in Africa and early agricultural Europe. Other aspects differ, including the impacts of steppe pastoralist expansions in West and East Eurasia and a near-complete turnover of Neolithic European dog ancestry

Reducing the environmental impact of surgery on a global scale: systematic review and co-prioritization with healthcare workers in 132 countries

Background Healthcare cannot achieve net-zero carbon without addressing operating theatres. The aim of this study was to prioritize feasible interventions to reduce the environmental impact of operating theatres. Methods This study adopted a four-phase Delphi consensus co-prioritization methodology. In phase 1, a systematic review of published interventions and global consultation of perioperative healthcare professionals were used to longlist interventions. In phase 2, iterative thematic analysis consolidated comparable interventions into a shortlist. In phase 3, the shortlist was co-prioritized based on patient and clinician views on acceptability, feasibility, and safety. In phase 4, ranked lists of interventions were presented by their relevance to high-income countries and low–middle-income countries. Results In phase 1, 43 interventions were identified, which had low uptake in practice according to 3042 professionals globally. In phase 2, a shortlist of 15 intervention domains was generated. In phase 3, interventions were deemed acceptable for more than 90 per cent of patients except for reducing general anaesthesia (84 per cent) and re-sterilization of ‘single-use’ consumables (86 per cent). In phase 4, the top three shortlisted interventions for high-income countries were: introducing recycling; reducing use of anaesthetic gases; and appropriate clinical waste processing. In phase 4, the top three shortlisted interventions for low–middle-income countries were: introducing reusable surgical devices; reducing use of consumables; and reducing the use of general anaesthesia. Conclusion This is a step toward environmentally sustainable operating environments with actionable interventions applicable to both high– and low–middle–income countries

Reducing the environmental impact of surgery on a global scale: systematic review and co-prioritization with healthcare workers in 132 countries

Abstract Background Healthcare cannot achieve net-zero carbon without addressing operating theatres. The aim of this study was to prioritize feasible interventions to reduce the environmental impact of operating theatres. Methods This study adopted a four-phase Delphi consensus co-prioritization methodology. In phase 1, a systematic review of published interventions and global consultation of perioperative healthcare professionals were used to longlist interventions. In phase 2, iterative thematic analysis consolidated comparable interventions into a shortlist. In phase 3, the shortlist was co-prioritized based on patient and clinician views on acceptability, feasibility, and safety. In phase 4, ranked lists of interventions were presented by their relevance to high-income countries and low–middle-income countries. Results In phase 1, 43 interventions were identified, which had low uptake in practice according to 3042 professionals globally. In phase 2, a shortlist of 15 intervention domains was generated. In phase 3, interventions were deemed acceptable for more than 90 per cent of patients except for reducing general anaesthesia (84 per cent) and re-sterilization of ‘single-use’ consumables (86 per cent). In phase 4, the top three shortlisted interventions for high-income countries were: introducing recycling; reducing use of anaesthetic gases; and appropriate clinical waste processing. In phase 4, the top three shortlisted interventions for low–middle-income countries were: introducing reusable surgical devices; reducing use of consumables; and reducing the use of general anaesthesia. Conclusion This is a step toward environmentally sustainable operating environments with actionable interventions applicable to both high– and low–middle–income countries

EFFECTS OF ACCOMPANYING USE OF HEAT TREATED CORN GRAIN AND WHOLE SOYBEANS IN CONCENTRATES ON WEANED CALVES PERFORMANCES **

Abstract: The objective of this experiment was to evaluate the effects of simultaneous using heat-treated corn grain as basic energy source and heattreated whole soybeans as protein source, in diet for calves 60-120 days old. The main predicted effects were improved energy and nutrients utilization efficiency and production performances of calves at this age. Thirty-six weaned Holstein calves were used, there were three treatments using different concentrates, formulated as: concentrate with micronized corn grain and extruded whole soybeans (KTT); concentrate with non-treated corn grain and soybean meal (KTN); concentrate with non-treated corn grain and extruded whole soybeans (KS). The marked effects of simultaneous use of heat-treated corn grain and whole soybeans were determined for feed efficiency (feed conversion ratio, FCR was 6,2 % higher for calves on KTT than on KTN and 4,3 % higher than on KS treatment). Efficiency of energy utilization (MJ ME/kg of gain) was better for calves on diet with KTT concentrate (5.8 % and 5.3 % lower efficiency of metabolic energy utilization for KTN and KS treatment respectively). Consumed crude protein was better utilized for growth by calves on KTT diets, than other two treatments (10.8 % more CP/kg of growth for KTN, and 7.5% more for KS)

DOI:10.2298/ABS1003747О MORPHOLOGICAL AND MOLECULAR IDENTIFICATION OF POTATO CYST NEMATODE POPULATIONS IN SERBIA

Abstract- Quarantine species such as potato cyst nematodes Globodera rostochiensis and G. pallida are present in Serbia since 1999 and 2005, respectively. These nematodes are sibling species and their morphological identification is complex due to their morphometric overlap. The cysts from the localities of Kladnica, Šanac, Gojna Gora and Milatovići were grown on susceptible potato varieties and their morphological differences have been discussed. To avoid ambiguities in species morphological designation a duplex PCR method was chosen for a rapid and accurate species identification. The whole procedure, from DNA extraction to DNA isolation, can be performed in a single day

Thermomechanical behavior of comercial yellow gold alloy

With the development of science and technology, in the late 19th century, began the research and application of new alloys for making jewelry. By adding different amounts of Cu and Ag alloy of Au, as well as adding some new elements (Zn), alloys were obtained with different color spectrum (from red to yellow) and different technological and metallurgical characteristics. This paper aims to show thermomechanical behavior of commercial yellow Au alloys for making jewelry