Altered Desaturation and Elongation of Fatty Acids in Hormone-Sensitive Lipase Null Mice

Hormone-sensitive lipase (HSL) is expressed predominantly in adipose tissue, where it plays an important role in catecholamine-stimulated hydrolysis of stored lipids, thus mobilizing fatty acids. HSL exhibits broad substrate specificity and besides acylglycerides it hydrolyzes cholesteryl esters, retinyl esters and lipoidal esters. Despite its role in fatty acid mobilization, HSL null mice have been shown to be resistant to diet-induced obesity. The aim of this study was to define lipid profiles in plasma, white adipose tissue (WAT) and liver of HSL null mice, in order to better understand the role of this multifunctional enzyme

Diet-Independent Remodeling of Cellular Membranes Precedes Seasonally Changing Body Temperature in a Hibernator

Polyunsaturated fatty acids (PUFA) have a multitude of health effects. Their incorporation into membrane phospholipids (PL) is generally believed to depend directly on dietary influx. PL influence transmembrane protein activity and thus can compensate temperature effects; e.g. PL n-6 PUFA are thought to stabilize heart function at low body temperature (Tb), whereas long chain (>C18) n-3 PUFA may boost oxidative capacity. We found substantial remodeling of membranes in free-living alpine marmots which was largely independent of direct dietary supply. Organ PL n-6 PUFA and n-6 to n-3 ratios were highest at onset and end of hibernation after rapid increases during a brief transitional period prior to hibernation. In contrast, longer chain PL n-3 PUFA content was low at end of summer but maximal at end of hibernation. After termination of hibernation in spring, these changes in PL composition were rapidly reversed. Our results demonstrate selective trafficking of PUFA within the body, probably governed by a circannual endogenous rhythm, as hibernating marmots were in winter burrows isolated for seven months from food and external cues signaling the approaching spring. High concentrations of PL n-6 PUFA throughout hibernation are in line with their hypothesized function of boosting SERCA 2a activity at low Tb. Furthermore, we found increasing rate of rewarming from torpor during winter indicating increasing oxidative capacity that could be explained by the accumulation of long-chain PL n-3 PUFA. It may serve to minimize the time necessary for rewarming despite the increasing temperature range to be covered, because rewarming is a period of highest metabolic rate and hence production of reactive oxygen species. Considering the importance of PUFA for health our results may have important biomedical implications, as seasonal changes of Tb and associated remodeling of membranes are not restricted to hibernators but presumably common among endothermic organisms

Relationship of weather types on the seasonal and spatial variability of rainfall, runoff, and sediment yield in the western Mediterranean basin

Rainfall is the key factor to understand soil erosion processes, mechanisms, and rates. Most research was conducted to determine rainfall characteristics and their relationship with soil erosion (erosivity) but there is little information about how atmospheric patterns control soil losses, and this is important to enable sustainable environmental planning and risk prevention. We investigated the temporal and spatial variability of the relationships of rainfall, runoff, and sediment yield with atmospheric patterns (weather types, WTs) in the western Mediterranean basin. For this purpose, we analyzed a large database of rainfall events collected between 1985 and 2015 in 46 experimental plots and catchments with the aim to: (i) evaluate seasonal differences in the contribution of rainfall, runoff, and sediment yield produced by the WTs; and (ii) to analyze the seasonal efficiency of the different WTs (relation frequency and magnitude) related to rainfall, runoff, and sediment yield. The results indicate two different temporal patterns: the first weather type exhibits (during the cold period: autumn and winter) westerly flows that produce the highest rainfall, runoff, and sediment yield values throughout the territory; the second weather type exhibits easterly flows that predominate during the warm period (spring and summer) and it is located on the Mediterranean coast of the Iberian Peninsula. However, the cyclonic situations present high frequency throughout the whole year with a large influence extended around the western Mediterranean basin. Contrary, the anticyclonic situations, despite of its high frequency, do not contribute significantly to the total rainfall, runoff, and sediment (showing the lowest efficiency) because of atmospheric stability that currently characterize this atmospheric pattern. Our approach helps to better understand the relationship of WTs on the seasonal and spatial variability of rainfall, runoff and sediment yield with a regional scale based on the large dataset and number of soil erosion experimental stations

Relationship of Weather Types on the Seasonal and Spatial Variability of Rainfall, Runoff, and Sediment Yield in the Western Mediterranean Basin

Rainfall is the key factor to understand soil erosion processes, mechanisms, and rates. Most research was conducted to determine rainfall characteristics and their relationship with soil erosion (erosivity) but there is little information about how atmospheric patterns control soil losses, and this is important to enable sustainable environmental planning and risk prevention. We investigated the temporal and spatial variability of the relationships of rainfall, runoff, and sediment yield with atmospheric patterns (weather types, WTs) in the western Mediterranean basin. For this purpose, we analyzed a large database of rainfall events collected between 1985 and 2015 in 46 experimental plots and catchments with the aim to: (i) evaluate seasonal differences in the contribution of rainfall, runoff, and sediment yield produced by the WTs; and (ii) to analyze the seasonal efficiency of the different WTs (relation frequency and magnitude) related to rainfall, runoff, and sediment yield. The results indicate two different temporal patterns: the first weather type exhibits (during the cold period: autumn and winter) westerly flows that produce the highest rainfall, runoff, and sediment yield values throughout the territory; the second weather type exhibits easterly flows that predominate during the warm period (spring and summer) and it is located on the Mediterranean coast of the Iberian Peninsula. However, the cyclonic situations present high frequency throughout the whole year with a large influence extended around the western Mediterranean basin. Contrary, the anticyclonic situations, despite of its high frequency, do not contribute significantly to the total rainfall, runoff, and sediment (showing the lowest efficiency) because of atmospheric stability that currently characterize this atmospheric pattern. Our approach helps to better understand the relationship of WTs on the seasonal and spatial variability of rainfall, runoff and sediment yield with a regional scale based on the large dataset and number of soil erosion experimental stations.Spanish Government (Ministry of Economy and Competitiveness, MINECO) and FEDER Projects: CGL2014 52135-C3-3-R, ESP2017-89463-C3-3-R, CGL2014-59946-R, CGL2015-65569-R, CGL2015-64284-C2-2-R, CGL2015-64284-C2-1-R, CGL2016-78075-P, GL2008-02879/BTE, LEDDRA 243857, RECARE-FP7, CGL2017-83866-C3-1-R, and PCIN-2017-061/AEI. Dhais Peña-Angulo received a “Juan de la Cierva” postdoctoral contract (FJCI-2017-33652 Spanish Ministry of Economy and Competitiveness, MEC). Ana Lucia acknowledge the "Brigitte-Schlieben-Lange-Programm". The “Geoenvironmental Processes and Global Change” (E02_17R) was financed by the Aragón Government and the European Social Fund. José Andrés López-Tarazón acknowledges the Secretariat for Universities and Research of the Department of the Economy and Knowledge of the Autonomous Government of Catalonia for supporting the Consolidated Research Group 2014 SGR 645 (RIUS- Fluvial Dynamics Research Group). Artemi Cerdà thank the funding of the OCDE TAD/CRP JA00088807. José Martínez-Fernandez acknowledges the project Unidad de Excelencia CLU-2018-04 co-funded by FEDER and Castilla y León Government. Ane Zabaleta is supported by the Hydro-Environmental Processes consolidated research group (IT1029-16, Basque Government). This paper has the benefit of the Lab and Field Data Pool created within the framework of the COST action CONNECTEUR (ES1306)

Site-specific differences in fatty acid composition of dendritic cells and associated adipose tissue in Popliteal Depot, Mesentery, and Omentum and their modulation by chronic inflammation and dietary lipids

Background: This study explores the role of lymphatics-associated adipocytes in determining the lipid composition of dendritic cells. Methods and Results: Adult male rats were fed plain chow, or chow supplemented with 20% sunflower or fish oil. Chronic local inflammation was induced by subcutaneous injection of 20 µg lipopolysaccharide three times a week for 2 weeks near the popliteal lymph nodes. Chemokine-stimulated dendritic cells were collected over 4 h from popliteal and mesenteric lymph nodes, and perinodal and other samples of mesenteric, popliteal and omental adipose tissue. Fatty acids extracted from triacylglycerols and/or phospholipids were separated and quantified by gas chromatography from each sample of dendritic cells and intracellular lipids, membranes, stroma and isolated adipocytes from the adipose tissue. Dendritic cells from lymph nodes and adipose tissue samples differ in fatty acid composition, and can be modulated by diet. The site-specific differences of dendritic cells correlate with those of the contiguous adipocytes. Chronic mild stimulation alters the lipid composition of dendritic cells near the inflamed site and elsewhere; changes are minimal after the fish-oil diet. The composition of adipocyte triacylglycerol and phospholipid fatty acids also changes near the stimulation site in ways that counteract alterations induced by the experimental diets. Conclusions: Fatty acids in dendritic cells differ with anatomical site, and are determined by the adjacent adipocytes, which actively regulate their own lipid composition. These findings demonstrate functional bases for the anatomical associations between adipose and lymphoid tissues and may be a mechanism by which dietary lipids modulate the immune system

MHYDAS-Erosion : a distributed single-storm water erosion model for agricultural catchments

In this paper, we present MHYDAS-Erosion, a dynamic and distributed single-storm water erosion model developed as a module of the existing hydrological MHYDAS model. As with many catchment erosion models, MHYDAS-Erosion is able to simulate sediment transport, erosion and deposition by rill and interrill processes. Its originality stems from its capacity to integrate the impact of land management practices (LMP) as key elements controlling the sedimentological connectivity in agricultural catchments. To this end, the water-sediment pathways are first determined by a specific process-oriented procedure defined and controlled by the user, which makes the integration of LMP easier. The LMP dynamic behaviours are then integrated into the model as a time-dependent function of hydrological variables and LMP characteristics. The first version of the model was implemented for vegetative filters and tested using water and sediment discharge measurements at three nested scales of a densely instrumented catchment (Roujan, OMERE Observatory, southern France). The results of discharge and soil loss for simulated rainfall events have been found to acceptably compare with available data. The average R-2 values for water and sediment discharge are 0.82 and 0.83, respectively. The sensitivity of the model to changes in the proportion of LMP was assessed for a single rain event by considering three scenarios of the Roujan catchment management with vegetative filters: 0% (Scenario 1), 18% (Scenario 2, real case) and 100% (Scenario 3). Compared to Scenario 2 (real case), soil losses decreased for Scenario 3 by 65% on the agricultural plot scale, 62% on the sub-catchment scale and 45% at the outlet of the catchment and increased for Scenario 1 by 0% on the plot scale, 26% on the sub-catchment scale and 18% at the outlet of the catchment