Ultrasonography of the prostate gland and testes in dogs

Ultrasonographic imaging is an important diagnostic tool because it allows assessment of the shape, size, position, margination and internal architecture of organs, as well as facilitating the study of vascular supply and vascularisation. Recently, there has been considerable development of B-mode, Doppler and contrast-enhanced ultrasonography for examination of the reproductive tract of dogs, both for studying normal physiology and in the clinical setting. This article describes the practical examination of the canine prostate gland and testes using a variety of ultrasound techniques, and details the normal appearance and blood flow of these organs as well as changes that may be observed with common reproductive disorders

Digital image analysis of testicular and prostatic ultrasonographic echogencity and heterogeneity in dogs and the relation to semen quality

A semi-automated ultrasonographic method was developed to measure echogenicity and heterogeneity of the testes and prostate gland and relationships of these measures with semen quality were assessed in 43 fertile dogs. The relationship between animal age and body weight upon the volume of the testes, epididymal tail volume and prostate volume were also established. Mean testicular echogenicity was negatively correlated with the percentage of morphologically normal live spermatozoa (more echogenic testes were associated with fewer normal sperm) but not with any other semen quality measure. Mean testicular heterogeneity was positively correlated with the total spermatozoal output (more heterogenous testes, being those with anechoic parenchyma and prominent echogenic stippling, were associated with greater sperm output) but not with any other semen quality measure. There was no relationship between either mean prostatic echogenicity or mean prostatic heterogeneity and any semen quality measure. There was no relationship between age and any testicular or prostatic parameter; however bodyweight was significantly correlated with total testicular volume, total epididymal tail volume and total prostatic volume. Testicular and prostatic ultrasonographic echogenicity and heterogeneity can be objectively assessed using digital image analysis and testicular echogenicity and heterogeneity may be useful adjunct measurements in a breeding soundness examination

Luteal phase decrease in packed cell volume in healthy non‐pregnant and pregnant bitches

Objectives: To establish packed cell volume (PCV) ranges for non‐pregnant, pregnant and post‐partum bitches from day 10 of proestrus, investigating any relationship with parity and litter size. Methods: This prospective cohort study used 37 healthy breeding bitches to examine PCV counts from routine blood samples collected every 4 weeks, from day 10 of proestrus, as part of routine PCV monitoring. Results: For pregnant (n = 19) and non‐pregnant (n = 18) bitches, PCV decreased until week 8 (corresponding to 8.5 ± 1.1 days before whelping for pregnant bitches) and recovered by 16–20 weeks after the initial sample; bitches that whelped average and large litters showed greater declines. PCV began to recover sooner for bitches that had previously whelped one or two litters compared to bitches that had previously whelped three or more litters. There was a significant three‐way interaction between time after the onset of proestrus, litter size and the number of previous litters which demonstrated that the large decrease in PCV for bitches that had previously whelped three or more litters only occurred in bitches that were expecting an average or large sized litter. Clinical Significance: Chronological variation in PCV for pregnant and non‐pregnant bitches was established during the reproductive cycle. There was no evidence to suggest that routine PCV measurement for normal, healthy bitches would be beneficial. However, knowledge from this study may be useful when deciding whether to prospectively monitor a bitch where there is a history of previous pregnancy‐related anaemia, when performing a caesarean section due to the anticipated blood loss during surgery, or when examining blood profiles for post‐litter bitches

Prevalence of ultrasound-determined cystic endometrial hyperplasia and the relationship with age in dogs

To investigate the potential relationship between age and diagnosis of cystic endometrial hyperplasia (CEH) in the bitches, 348 ultrasound examinations from 240 bitches (Labradors, Golden Retrievers, German Shepherds, Flat Coated Retrievers, or crosses of those breeds aged between 1.6 and 7.2 years at examination) were examined. A subpopulation of 32 bitches that had completed their breeding careers at 6 years or more of age was also identified. Of all, 18.3% of the bitches were diagnosed with CEH; these cases were newly diagnosed when bitches were between 2.5 years and 7.3 years of age. The proportion of ultrasound examinations in which CEH was identified increased from 6.8% of examinations on 2-year-old breeding bitches to 60.0% of examinations on 6-year-old bitches. Logistic regression identified a positive correlation between mean age at the examination and presence of CEH (χ2 = 30.74, degrees of freedom = 1, P < 0.001). For 32 bitches that had completed their breeding career, the prevalence of CEH was 56.3%, age at the diagnosis ranged from 3.8 to 7.3 years, and the proportion of bitches affected with CEH increased from 6.3% at 3 years of age to 56.3% at 7 years of age. These data support the contention that the prevalence of CEH increases with age

Habilitation provision for children and young people with vision impairment in the United Kingdom: A lack of clarity leading to inconsistencies

The key to empowering and supporting children and young people (CYP) with vision impairment (VI) to achieve their potential lies in the delivery of habilitation training. Evidence has revealed that provision of habilitation services across the United Kingdom was inconsistent, with CYP with VI not receiving services in some areas. This research explored the accessibility and quality of habilitation provision for CYP with VI via two studies: (1) 12 qualitative case studies of habilitation practice and (2) surveys of habilitation training experiences, with CYP with VI (n = 43) and with parents of CYP with VI (n = 68). Five themes were identified highlighting inconsistencies and variability in the delivery of habilitation training in recent years, a lack of focus on independent living skills training, on social inclusion and emotional well-being, a lack of support for parents and a lack of clarity with regard to the definition of habilitation, and who is responsible for providing training

Mycolactone-dependent depletion of endothelial cell thrombomodulin is strongly associated with fibrin deposition in Buruli ulcer lesions

A well-known histopathological feature of diseased skin in Buruli ulcer (BU) is coagulative necrosis caused by the Mycobacterium ulcerans macrolide exotoxin mycolactone. Since the underlying mechanism is not known, we have investigated the effect of mycolactone on endothelial cells, focussing on the expression of surface anticoagulant molecules involved in the protein C anticoagulant pathway. Congenital deficiencies in this natural anticoagulant pathway are known to induce thrombotic complications such as purpura fulimans and spontaneous necrosis. Mycolactone profoundly decreased thrombomodulin (TM) expression on the surface of human dermal microvascular endothelial cells (HDMVEC) at doses as low as 2ng/ml and as early as 8hrs after exposure. TM activates protein C by altering thrombin's substrate specificity, and exposure of HDMVEC to mycolactone for 24 hours resulted in an almost complete loss of the cells' ability to produce activated protein C. Loss of TM was shown to be due to a previously described mechanism involving mycolactone-dependent blockade of Sec61 translocation that results in proteasome-dependent degradation of newly synthesised ER-transiting proteins. Indeed, depletion from cells determined by live-cell imaging of cells stably expressing a recombinant TM-GFP fusion protein occurred at the known turnover rate. In order to determine the relevance of these findings to BU disease, immunohistochemistry of punch biopsies from 40 BU lesions (31 ulcers, nine plaques) was performed. TM abundance was profoundly reduced in the subcutis of 78% of biopsies. Furthermore, it was confirmed that fibrin deposition is a common feature of BU lesions, particularly in the necrotic areas. These findings indicate that there is decreased ability to control thrombin generation in BU skin. Mycolactone's effects on normal endothelial cell function, including its ability to activate the protein C anticoagulant pathway are strongly associated with this. Fibrin-driven tisischemia could contribute to the development of the tissue necrosis seen in BU lesions

Effect of timing of neutering in relation to puberty on behaviour in the female dog – a scoping review

Scoping review protoco

A prospective cohort study investigating the peri- and postoperative outcomes following ovariohysterectomy in bitches neutered prepubertally or post-pubertally.

The aim of this prospective cohort study was to investigate perioperative and postoperative outcomes for bitches that were neutered at different times in relation to puberty (rather than age). Three hundred and six Labrador and Golden Retriever crossbreed bitches were neutered before (prepubertal neuter [PPN], n = 155) or after puberty (Control, n = 151) by experienced veterinarians at one of four veterinary practices (VP1-4). Data were gathered from veterinarians at the time of neutering and from bitch caregivers for the 14-day period after neutering using purpose-designed questionnaires. PPN bitches had shorter surgery durations than Control bitches (OR = 0.877, 91% CI = 0.800 to 0.961, P = 0.015). Longer surgery durations were reported for bitches that had intraoperative complications (OR = 1.620, 91% CI = 1.397 to 1.879, P &lt; 0.001). Shorter surgery durations were reported for VP1 compared to all other veterinary practices (OR = 1.189 to 1.534, P &lt; 0.001). There was no difference in the number of PPN or Control bitches that had perioperative complications (PPN: 3.9%, n = 6; Control: 7.7%, n = 11) and no bitch had more than one perioperative complication reported. For the 14-day postoperative period, fewer PPN (1.3%, n = 2) compared to Control bitches (8.9%, n = 13) had wound discharge (Chi-square = 11.151, D.F = 1, P = 0.001) or showed inappetence (PPN: 2.9%, n = 4; Control: 17.3%, n = 19, Chi-square = 14.884, D.F. = 1, P &lt; 0.001). There was no difference in the number of PPN and Control bitches that had redness or swelling of the wound (52 PPN, 48 Control), showed attention to the wound (6 PPN, 6 Control), required veterinary attention (25 PPN, 23 Control), or that showed any other unusual behaviours: discomfort (27 PPN, 16 Control), uncharacteristic irritability (2 PPN, 4 Control), or uncharacteristic licking or chewing of parts of the body (15 PPN, 6 Control). Bitches neutered at VP2-4 were more likely to have wound redness or swelling reported than those neutered at VP1 (OR = 3.109 to 4.076, P = 0.003). Future studies should consider the impact of veterinary practice on results. These results for peri and postoperative complications could suggest that prepubertal ovariohysterectomy may be a suitable option for neutering bitches. However, it is recommended that they be interpreted together with evidence relating to the impacts on other factors including behaviour and longer-term health in order to make fully informed decisions

A prospective cohort study investigating the behavioural development of bitches in a guide dog training programme neutered prepubertally or post-pubertally

There are few studies which investigate the effect of neutering bitches before or after puberty. The majority of current literature examining the impact of the timing of neutering on health and behaviour has used age rather than onset of puberty as the key variable. The aim of this prospective cohort study was to investigate the effects of timing of neutering in relation to puberty on behaviour in female dogs reared and trained in an assistance dog programme. The study examined data for bitches neutered before or after puberty to compare scores for six behavioural factors (Training and obedience, Aggression, Fear and anxiety, Excitability, Attachment and attention-seeking, Social behaviour) measured at one and three-years of age using an adapted version of the Canine Behavioral Assessment and Research Questionnaire (C-BARQ). Labrador and Golden Retriever crossbreed bitches were neutered before (n=155) or after (n=151) puberty. Neutering before or after puberty had no impact on mean scores for the six behavioural factors at one or three years of age. When examining the change in behavioural factor scores between one and three years of age, only Aggression behavioural factor scores were influenced by neutering before or after puberty. Bitches neutered after puberty were less likely to have Aggression factor scores which increased between one and three years of age (OR = 0.959, 90% CI = 0.924 to 0.995, P = 0.06). However, the majority of bitches scored ‘0’ for Aggression at both time points (indicating no aggression behaviours were observed) and the number of bitches for which scores increased between one and three years of age was low (before puberty = 20, after puberty = 9). This is consistent with very mild aggressive behaviours being observed in a small number of animals and is therefore of questionable concern. The results suggest that for Labrador and Golden Retriever crossbreed bitches, neutering before or after puberty has little to no effect on future behaviour. It is recommended that decisions about the timing of neutering are not informed solely by impacts on behaviour, but that they also consider evidence relating to the impacts on bitch health and wellbeing

Effect of neutering timing in relation to puberty on female dog behaviour—a scoping review

Background: There are contradictory findings in the literature relating to the effects of timing of neutering on bitch behaviour that make decision making regarding neutering timing difficult. Methods: A scoping review was designed to identify and chart the evidence on the effect of neutering timing in relation to puberty on the behaviour of female domesticated dogs. A protocol was registered, and literature searches were conducted in CAB Abstracts, Medline and Web of Science. Studies were reviewed against the inclusion criteria. Data on study and population characteristics and behavioural outcomes were extracted for the final included studies. Results: A total of 1048 publications were reviewed; 13 were retained for inclusion and charting. Only one of the two studies that classified bitches as pre- or postpubertal presented results for the analysis of behaviours. The remaining 11 studies classified bitches by age at neutering. Limitations: Since the scoping review searches were conducted, further relevant studies may have become available. The search strategy may not have identified all available literature; however, databases were used that provide high levels of coverage of veterinary literature. Conclusion: This scoping review identified a lack of evidence to document the impact of neutering bitches before or after puberty on behaviour