4,490 research outputs found
First complete Providencia rettgeri genome sequence, the NDM-1-producing clinical strain RB151
© 2017 Marquez-Ortiz et al. Providencia rettgeri is an opportunistic bacterial pathogen of clinical significance due to its association with urinary tract infections and multidrug resistance. Here, we report the first complete genome sequence of P. rettgeri. The genome of strain RB151 consists of a 4.8-Mbp chromosome and a 108-kbp blaNDM-1-positive plasmid
Plan de negocios de una plataforma digital para la gesti?n de la trazabilidad para las cooperativas cafetaleras del Per?
El presente estudio eval?a la viabilidad comercial, operativa y econ?mica para la puesta en marcha de una plataforma digital que facilitara el proceso de trazabilidad en las cooperativas de caf? de San Martin, El modelo de negocio plantea la creaci?n de Agro Nativo Digital, plataforma de apoyo a la gesti?n de la trazabilidad en las cooperativas exportadoras de caf?, ofreciendo un servicio especializado, permitiendo reducci?n de los costos, tiempo e informaci?n ordenada y disponible digitalizando la informaci?n de campo. El modelo no s?lo se apalanca en una variable tecnol?gica o de especializaci?n, tambi?n en el cumplimiento de la inocuidad alimentaria. Se da un valor agregado y diferencial para el cliente, mediante un reporte de conformidad de trazabilidad digital al cual el cliente puede tener acceso desde cualquier parte del mundo y pudiendo verificar los insumos utilizados en cada lote (agua, insumos qu?micos, huellas de carbono). La evaluaci?n econ?mica del plan de negocio en funci?n a las variables anteriormente descritas y explicadas y con una tasa de descuento de 25% para flujos econ?micos, resulta positiva con un VAN ECONOMICO de S/ 171,068 soles mayor a 0 y una TIR de 39.41% mayor al Koa 25%, por lo que se considera viable econ?micamente y se recomienda su aprobaci?n
Retinal nerve fibre layer thickness profile in normal eyes using third-generation optical coherence tomography
Aims To establish four normal retinal nerve fibre layer (RNFL) thickness radial profiles based on third-generation optical coherence tomography (OCT) and to compare them with previously reported histologic measurements.Methods A total of 20 normal eyes were studied. A circular scan was adjusted to the size of the optic disc and three scans were performed with this radius and every 200 mu m thereafter, up to a distance of 1400 mu m. Four different radial sections (superotemporal, superonasal, inferonasal, and inferotemporal) were studied to establish RNFL thickness OCT profiles. Additionally, two radial scans orientated at 45 and 1351 crossing the optic disc centre were performed in six of 20 eyes, and RNFL thickness was measured at disc margin.Results Quadrant location and distance from disc margin interaction in RNFL thickness was statistically significant (P < 0.001). the RNFL thickness decreased (P < 0.001) as the distance from the disc margin increased for all sections. the measurements automatically generated by the OCT built-in software were thinner (P < 0.001) than histologic ones close to the disc margin.Conclusions Four normal OCT RNFL profiles were established and compared with histological data obtained from the same area. RNFL measurements assessed by OCT 3 were significantly thinner close to the optic disc margin.Hosp Olhos Araraquara, Glaucoma Sect, BR-14802530 Araraquara, SP, BrazilHosp Olhos Araraquara, Retina Diagnost & Treatment Div, BR-14802530 Araraquara, SP, BrazilUniversidade Federal de São Paulo, São Paulo, BrazilUSP, Inst Fis Sao Carlos, Sao Carlos, SP, BrazilUniv So Calif, Doheny Eye Inst, Dept Ophthalmol, Los Angeles, CA USAUniversidade Federal de São Paulo, São Paulo, BrazilWeb of Scienc
The Antibacterial Activity of Honey Derived from Australian Flora
Chronic wound infections and antibiotic resistance are driving interest in
antimicrobial treatments that have generally been considered complementary,
including antimicrobially active honey. Australia has unique native flora and
produces honey with a wide range of different physicochemical properties. In
this study we surveyed 477 honey samples, derived from native and exotic plants
from various regions of Australia, for their antibacterial activity using an
established screening protocol. A level of activity considered potentially
therapeutically useful was found in 274 (57%) of the honey samples, with
exceptional activity seen in samples derived from marri (Corymbia
calophylla), jarrah (Eucalyptus marginata) and
jellybush (Leptospermum polygalifolium). In most cases the
antibacterial activity was attributable to hydrogen peroxide produced by the
bee-derived enzyme glucose oxidase. Non-hydrogen peroxide activity was detected
in 80 (16.8%) samples, and was most consistently seen in honey produced
from Leptospermum spp. Testing over time found the hydrogen
peroxide-dependent activity in honey decreased, in some cases by 100%,
and this activity was more stable at 4°C than at 25°C. In contrast, the
non-hydrogen peroxide activity of Leptospermum honey samples
increased, and this was greatest in samples stored at 25°C. The stability of
non-peroxide activity from other honeys was more variable, suggesting this
activity may have a different cause. We conclude that many Australian honeys
have clinical potential, and that further studies into the composition and
stability of their active constituents are warranted
Maternal neurofascin-specific autoantibodies bind to structures of the fetal nervous system during pregnancy, but have no long term effect on development in the rat
Neurofascin was recently reported as a target for axopathic autoantibodies in patients with multiple sclerosis (MS), a response that will exacerbate axonal pathology and disease severity in an animal model of multiple sclerosis. As transplacental transfer of maternal autoantibodies can permanently damage the developing nervous system we investigated whether intrauterine exposure to this neurofascin-specific response had any detrimental effect on white matter tract development. To address this question we intravenously injected pregnant rats with either a pathogenic anti-neurofascin monoclonal antibody or an appropriate isotype control on days 15 and 18 of pregnancy, respectively, to mimic the physiological concentration of maternal antibodies in the circulation of the fetus towards the end of pregnancy. Pups were monitored daily with respect to litter size, birth weight, growth and motor development. Histological studies were performed on E20 embryos and pups sacrificed on days 2, 10, 21, 32 and 45 days post partum. Results: Immunohistochemistry for light and confocal microscopy confirmed passively transferred anti-neurofascin antibody had crossed the placenta to bind to distinct structures in the developing cortex and cerebellum. However, this did not result in any significant differences in litter size, birth weight, or general physical development between litters from control mothers or those treated with the neurofascin-specific antibody. Histological analysis also failed to identify any neuronal or white matter tract abnormalities induced by the neurofascin-specific antibody. Conclusions: We show that transplacental transfer of circulating anti-neurofascin antibodies can occur and targets specific structures in the CNS of the developing fetus. However, this did not result in any pre- or post-natal abnormalities in the offspring of the treated mothers. These results assure that even if anti-neurofascin responses are detected in pregnant women with multiple sclerosis these are unlikely to have a negative effect on their children
Artificial graphene as a tunable Dirac material
Artificial honeycomb lattices offer a tunable platform to study massless
Dirac quasiparticles and their topological and correlated phases. Here we
review recent progress in the design and fabrication of such synthetic
structures focusing on nanopatterning of two-dimensional electron gases in
semiconductors, molecule-by-molecule assembly by scanning probe methods, and
optical trapping of ultracold atoms in crystals of light. We also discuss
photonic crystals with Dirac cone dispersion and topologically protected edge
states. We emphasize how the interplay between single-particle band structure
engineering and cooperative effects leads to spectacular manifestations in
tunneling and optical spectroscopies.Comment: Review article, 14 pages, 5 figures, 112 Reference
Rare germline variants in DNA repair genes and the angiogenesis pathway predispose prostate cancer patients to develop metastatic disease
Background
Prostate cancer (PrCa) demonstrates a heterogeneous clinical presentation ranging from largely indolent to lethal. We sought to identify a signature of rare inherited variants that distinguishes between these two extreme phenotypes.
Methods
We sequenced germline whole exomes from 139 aggressive (metastatic, age of diagnosis < 60) and 141 non-aggressive (low clinical grade, age of diagnosis ≥60) PrCa cases. We conducted rare variant association analyses at gene and gene set levels using SKAT and Bayesian risk index techniques. GO term enrichment analysis was performed for genes with the highest differential burden of rare disruptive variants.
Results
Protein truncating variants (PTVs) in specific DNA repair genes were significantly overrepresented among patients with the aggressive phenotype, with BRCA2, ATM and NBN the most frequently mutated genes. Differential burden of rare variants was identified between metastatic and non-aggressive cases for several genes implicated in angiogenesis, conferring both deleterious and protective effects.
Conclusions
Inherited PTVs in several DNA repair genes distinguish aggressive from non-aggressive PrCa cases. Furthermore, inherited variants in genes with roles in angiogenesis may be potential predictors for risk of metastases. If validated in a larger dataset, these findings have potential for future clinical application
What do -ray bursts look like?
There have been great and rapid progresses in the field of -ray
bursts (denoted as GRBs) since BeppoSAX and other telescopes discovered their
afterglows in 1997. Here, we will first give a brief review on the
observational facts of GRBs and direct understanding from these facts, which
lead to the standard fireball model. The dynamical evolution of the fireball is
discussed, especially a generic model is proposed to describe the whole
dynamical evolution of GRB remnant from highly radiative to adiabatic, and from
ultra-relativistic to non-relativistic phase. Then, Various deviations from the
standard model are discussed to give new information about GRBs and their
environment. In order to relax the energy crisis, the beaming effects and their
possible observational evidences are also discussed in GRB's radiations.Comment: 10 pages, Latex. Invited talk at the Pacific Rim Conference on
Stellar Astrophysics, Hong Kong, China, Aug. 199
The Effects of Cocaine on Different Redox Forms of Cysteine and Homocysteine, and on Labile, Reduced Sulfur in the Rat Plasma Following Active versus Passive Drug Injections
Received: 28 November 2012 / Revised: 19 April 2013 / Accepted: 6 May 2013 / Published online: 16 May 2013
The Author(s) 2013. This article is published with open access at Springerlink.comThe aim of the present studies was to evaluate
cocaine-induced changes in the concentrations of different
redox forms of cysteine (Cys) and homocysteine (Hcy),
and products of anaerobic Cys metabolism, i.e., labile,
reduced sulfur (LS) in the rat plasma. The above-mentioned
parameters were determined after i.p. acute and
subchronic cocaine treatment as well as following i.v.
cocaine self-administration using the yoked procedure.
Additionally, Cys, Hcy, and LS levels were measured
during the 10-day extinction training in rats that underwent
i.v. cocaine administration. Acute i.p. cocaine treatment
increased the total and protein-bound Hcy contents,
decreased LS, and did not change the concentrations of Cys
fractions in the rat plasma. In turn, subchronic i.p. cocaine administration significantly increased free Hcy and lowered
the total and protein-bound Cys concentrations while
LS level was unchanged. Cocaine self-administration
enhanced the total and protein-bound Hcy levels, decreased
LS content, and did not affect the Cys fractions. On the
other hand, yoked cocaine infusions did not alter the concentration
of Hcy fractions while decreased the total and
protein-bound Cys and LS content. This extinction training
resulted in the lack of changes in the examined parameters
in rats with a history of cocaine self-administration while in
the yoked cocaine group an increase in the plasma free Cys
fraction and LS was seen. Our results demonstrate for the
first time that cocaine does evoke significant changes in
homeostasis of thiol amino acids Cys and Hcy, and in some
products of anaerobic Cys metabolism, which are dependent
on the way of cocaine administration
Updating known distribution models for forecasting climate change impact on endangered species
To plan endangered species conservation and to design adequate management programmes, it is necessary to predict their
distributional response to climate change, especially under the current situation of rapid change. However, these
predictions are customarily done by relating de novo the distribution of the species with climatic conditions with no regard
of previously available knowledge about the factors affecting the species distribution. We propose to take advantage of
known species distribution models, but proceeding to update them with the variables yielded by climatic models before
projecting them to the future. To exemplify our proposal, the availability of suitable habitat across Spain for the endangered
Bonelli’s Eagle (Aquila fasciata) was modelled by updating a pre-existing model based on current climate and topography to
a combination of different general circulation models and Special Report on Emissions Scenarios. Our results suggested that
the main threat for this endangered species would not be climate change, since all forecasting models show that its
distribution will be maintained and increased in mainland Spain for all the XXI century. We remark on the importance of
linking conservation biology with distribution modelling by updating existing models, frequently available for endangered
species, considering all the known factors conditioning the species’ distribution, instead of building new models that are
based on climate change variables only.Ministerio de Ciencia e Innovación and FEDER (project CGL2009-11316/BOS
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