Indicadores bibliométricos de actividad de la revista MVZ Córdoba 1994-2008

Objetivo. Analizar el comportamiento bibliométrico de la revista MVZ-Córdoba y visualizar indicadores que permitan la toma de decisiones enfocadas a mejorar la calidad de la publicación. Materiales y métodos. Se emplearon los volúmenes del 1 al 13 compuestos por 49.2% de artículos originales, 17.4% de resúmenes de trabajos de grado, 17.7% de otros documentos, 8.9% de artículos de revisión, 4.3% de casos clínicos, 1.3% de artículos de opinión, 1.0% de comunicaciones breves, 0.3% de artículos institucionales, para un total de 305 manuscritos, 915 autores y 4881 referencias. Se determinaron los índices de colaboración (IC), productividad (IP), Price (IO), obsolescencia, aislamiento (IA), autocitación (SCR); también se evaluó la distribución porcentual y tipo de artículos, el número de autores y el promedio de citas. Resultados. Los índices generales IC, IP, IO, IA y SCR fueron 3.0, 2.18, 30.3, 7.67 y 8.9 % respectivamente; el análisis de Burton-Kebler reportó un envejecimiento anual que osciló entre 90.5 y 95.1%, lo que significa una pérdida de actualidad entre 4.93 y 9.52%. Conclusiones. La revista ha mostrado una calidad ascendente lo que se corrobora con la indización internacional. Para mantener y aumentar la calidad de la revista se debe reglamentar y verificar la actualidad de las referencias, controlar el SCR, el IA, aumentar el índice de Price, la vida media, realizar mediciones bibliométricas con mayor periodicidad, así como establecer la vigilancia sobre los índices bibliométricos que se generen de las indizaciones de la revista MVZ Córdoba

Low-Scale Expression and Purification of an Active Putative Iduronate 2-Sulfate Sulfatase-Like Enzyme from Escherichia coli K12

The sulfatase family involves a group of enzymes with a large degree of similarity. Until now, sixteen human sulfatases have been identified, most of them found in lysosomes. Human deficiency of sulfatases generates various genetic disorders characterized by abnormal accumulation of sulfated intermediate compounds. Mucopolysaccharidosis type II is characterized by the deficiency of iduronate 2-sulfate sulfatase (IDS), causing the lysosomal accumulation of heparan and dermatan sulfates. Currently, there are several cases of genetic diseases treated with enzyme replacement therapy, which have generated a great interest in the development of systems for recombinant protein expression. In this work we expressed the human recombinant IDS-Like enzyme (hrIDS-Like) in Escherichia coli DH5α. The enzyme concentration revealed by ELISA varied from 78. 13 to 94. 35 ng/ml and the specific activity varied from 34. 20 to 25. 97 nmol/h/mg. Western blotting done after affinity chromatography purification showed a single band of approximately 40 kDa, which was recognized by an IgY polyclonal antibody that was developed against the specific peptide of the native protein. Our 100 ml-shake-flask assays allowed us to improve the enzyme activity seven fold, compared to the E. coli JM109/pUC13-hrIDS-Like system. Additionally, the results obtained in the present study were equal to those obtained with the Pichia pastoris GS1115/pPIC-9-hrIDS-Like system (3 L bioreactor scale). The system used in this work (E. coli DH5α/pGEX-3X-hrIDS-Like) emerges as a strategy for improving protein expression and purification, aimed at recombinant protein chemical characterization, future laboratory assays for enzyme replacement therapy, and as new evidence of active putative sulfatase production in E. coli. © 2013 The Microbiological Society of Korea and Springer-Verlag Berlin Heidelberg.Fil: Morales Álvarez, Edwin David. Universidad del Quindio; ColombiaFil: Rivera Hoyos, Claudia Marcela. Universidad del Quindio. Facultad de Medicina; ColombiaFil: Baena Moncada, Angelica Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Landázuri, Patricia. Universidad del Quindio. Facultad de Medicina. Centro de Investig. Biomédicas; ColombiaFil: Poutou Piñales, Raúl A.. Pontificia Universidad Javeriana; ColombiaFil: Sáenz Suárez, Homero. Universidad del Quindio; ColombiaFil: Barrera, Luis A.. Pontificia Universidad Javeriana; ColombiaFil: Echeverri Peña, Olga Y.. Pontificia Universidad Javeriana; Colombi

Production of recombinant trichoderma reesei cellobiohydrolase ii in a new expression system based on wickerhamomyces anomalus

Q31-7Cellulase is a family of at least three groups of enzymes that participate in the sequential hydrolysis of cellulose. Recombinant expression of cellulases might allow reducing their production times and increasing the low proteins concentrations obtained with filamentous fungi. In this study, we describe the production of Trichoderma reesei cellobiohydrolase II (CBHII) in a native strain of Wickerhamomyces anomalus. Recombinant CBHII was expressed in W. anomalus 54-A reaching enzyme activity values of up to 14.5 U L−1. The enzyme extract showed optimum pH and temperature of 5.0–6.0 and 40°C, respectively. Enzyme kinetic parameters ( of 2.73 mM and Vmax of 23.1 µM min−1) were between the ranges of values reported for other CBHII enzymes. Finally, the results showed that an enzymatic extract of W. anomalus 54-A carrying the recombinant T. reesei CBHII allows production of reducing sugars similar to that of a crude extract from cellulolytic fungi. These results show the first report on the use of W. anomalus as a host to produce recombinant proteins. In addition, recombinant T. reesei CBHII enzyme could potentially be used in the degradation of lignocellulosic residues to produce bioethanol, based on its pH and temperature activity profile

Producción de a-Amilasa con celulas libres e inmovilizadas de Thermus sp.

The thermostable á-amylase production is compared, using a native strain of Thermus sp., in batch culture and immobilized cells in 3% p/v of sodium alginate. The production was carried out in PAP2 broth modified by the addition of maize starch in a concentration of 3.024g/l. The results obtained in reactor, reported greater efficiency due to the geometric configuration; concentration of the carbon source, oxygenation and temperature; allowing better substrate assimilation. The maximum production of alpha amylase was obtained with immobilized cells in fermentor with 1l of culture broth (fluidized culture), at 24 hours of fermentation (360.97UA/min l and 149.09 of specific activity), vs., 60.31UA/min l and 18.40 of specific activity, obtained in reactor with 10l working volume at 14h. It is important to remark in this work, which is the first time that immobilized cells of Thermus sp., are used for the production of thermostable alpha-amylase, in Colombia.Se compara la producción de á-amilasa termoestable a partir de almidón, empleando una cepa autóctona de Thermus sp., en cultivo discontinuo con células libres e inmovilizadas en 3% p/v de alginato de sodio. La producción se llevó a cabo en medio PAP2 modificado por la adición de almidón de maíz a 3.024g/l. Los resultados obtenidos en el fermentador, reportaron mayor eficiencia debido a la configuración geométrica; concentración de la fuente de carbono, oxigenación y temperatura; lo que permite mejor aprovechamiento del sustrato. La máxima producción de alpha amilasa se obtuvo con células inmovilizadas en fermentador con 1l de caldo de cultivo (cultivo fluidizado), a las 24 horas de fermentación (360.97UA/min l y 149.09 de actividad específica), vs., 60.31UA/min l y 18.40 de actividad específica, obtenida en fermentador con 10l de volumen de trabajo a las 14h. Es importante resaltar de este trabajo, que por primera vez en Colombia son utilizadas células inmovilizadas de Thermus sp., para la producción de alpha amilasa termoestable

Antimicrobial Resistance of Non-Typhoid Salmonella in Meat and Meat Products

Salmonella enterica serovars are associated with numerous annual deaths worldwide and are responsible for a large number of foodborne diseases. Within this frame of reference, knowledge of antimicrobial susceptibility represents the fundamental approach of most Salmonella treatments. Therefore, scientific publications of antimicrobial susceptibilities and resistance must be precise, with interpretations adjusted to a particular standard. Hence, the three objectives in this study were: (i) to describe the frequency of antimicrobial-resistant isolates of Non-Typhoidal Salmonella (NTS) isolated from beef, pork, chicken meat, and other meat products; (ii) to describe the distribution of serovars and their multi-resistance to antibiotics for clinical use (veterinary and human) between 1996 and 2019; and (iii) to propose additional considerations that could improve the use and usefulness of the published results. Our results determined that the predominant isolates came from poultry. Enteritidis and Typhimurium were the most reported serovars by MIC (with both having the highest resistance to TET) while the lowest resistance was to CIP and CRO for Enteritidis and Typhimurium, respectively. The multi-resistance pattern AMP AMC CEP GEN KAN STR TET was the most frequently observed pattern by MIC in Montevideo and Seftenberg, while, for disc diffusion, the pattern AMP STR TET was the most frequent in the Bredeney serotype. In conclusion, researchers should carry out homogeneous sampling procedures, identify the types of the samples, use standard identification methods, and employ appropriate standards for antimicrobial susceptibility interpretation. Additionally, there is also a need for all WHO members to comply with the WHA 73.5 resolution. Our final recommendation is for all producers to reduce antibiotic prophylactic use

A Brief History of Colour, the Environmental Impact of Synthetic Dyes and Removal by Using Laccases

The history of colour is fascinating from a social and artistic viewpoint because it shows the way; use; and importance acquired. The use of colours date back to the Stone Age (the first news of cave paintings); colour has contributed to the social and symbolic development of civilizations. Colour has been associated with hierarchy; power and leadership in some of them. The advent of synthetic dyes has revolutionized the colour industry; and due to their low cost; their use has spread to different industrial sectors. Although the percentage of coloured wastewater discharged by the textile; food; pharmaceutical; cosmetic; and paper industries; among other productive areas; are unknown; the toxic effect and ecological implications of this discharged into water bodies are harmful. This review briefly shows the social and artistic history surrounding the discovery and use of natural and synthetic dyes. We summarise the environmental impact caused by the discharge of untreated or poorly treated coloured wastewater to water bodies; which has led to physical; chemical and biological treatments to reduce the colour units so as important physicochemical parameters. We also focus on laccase utility (EC 1.10.3.2), for discolouration enzymatic treatment of coloured wastewater, before its discharge into water bodies. Laccases (p-diphenol: oxidoreductase dioxide) are multicopper oxidoreductase enzymes widely distributed in plants, insects, bacteria, and fungi. Fungal laccases have employed for wastewater colour removal due to their high redox potential. This review includes an analysis of the stability of laccases, the factors that influence production at high scales to achieve discolouration of high volumes of contaminated wastewater, the biotechnological impact of laccases, and the degradation routes that some dyes may follow when using the laccase for colour remova

Surveillance of Levofloxacin Resistance in Helicobacter pylori Isolates in Bogotá-Colombia (2009-2014).

Increased resistance of Helicobacter pylori to clarithromycin and metronidazole has resulted in recommendation to substitute fluoroquinolones for eradication therapy. The aims of the study were to determine the prevalence and changes in primary levofloxacin resistance related to H. pylori gyrA sequences. The study utilized H. pylori strains isolated from patients undergoing gastroscopy in Bogotá, Colombia from 2009 to 2014. Levofloxacin susceptibility was assessed by agar dilution. Mutations in gyrA sequences affecting the quinolone resistance-determining region (QRDR) were evaluated by direct sequencing. Overall, the mean prevalence of primary levofloxacin resistance was 18.2% (80 of 439 samples). Resistance increased from 11.8% (12/102) in 2009 to 27.3% (21/77) in 2014 (p = 0.001). gyrA mutations in levofloxacin resistant strains were present in QRDR positions 87 and 91. The most common mutation was N87I (43.8%, 35/80) followed by D91N (28.8%, 23/80) and N87K (11.3%, 9/80). Levofloxacin resistance increased markedly in Colombia during the six-year study period. Primary levofloxacin resistance was most often mediated by point mutations in gyrA, with N87I being the most common QRDR mutation related to levofloxacin resistance

Human sulfatase transiently and functionally active expressed in E. coli K12

The recombinant human iduronate 2-sulfate sulfatase (hrIDS) was transiently and functionally active expressed in E. coli K12. The enzyme activity (crude extract) at 100 ml and 400 ml oscillated between 0.25 and 10.58 nmol h-1 mg-1. The wide Western-blot peptide profile suggest that hrIDS is proteolitically processed “randomly” which agrees with the ultrafiltration assay in which the hrIDS activity was found in all fractions (100kDa). No glycation sites were found by computer analysis of the hIDS sequence; discarding the possibility of marks for glycation and proteolytic processing

Influencia de la fuente de carbono sobre la expresión de proteínas AOX1-reguladas en Pichia pastoris

Influence of carbon source over the expression of AOX1-regulated proteins in Pichia pastoris: The Pichia pastoris yeast is an excellent model for the expression of heterologous protein, thus could be deduce from the greate number of proteins that has been obtain in this biological model under the control of AOX1 promoter. This means that peroxisomes number and the enzyme alcohol oxidase are regulate as response to the induction of different metabolites such as methanol, glycerol, ethanol, acetate and metabolic pathways like the b- oxidation. In this review, aspects related with the metabolism of this compounds and its possible influence in the production of recombinant proteins are discuss, specifically the human Iduronate 2-sulfate sulfatase (hIDS).La levadura Pichia pastoris constituye un excelente modelo para la expresión de proteínas heterólogas, lo que se refleja en el gran número de proteínas que han sido obtenidas en este modelo biológico, bajo el control del promotor AOX1. Esto implica que el número de peroxisomas y la enzima alcohol oxidasa es regulada como respuesta a la inducción por metabolitos como el metanol, el glicerol, el etanol, el acetato y vías metabólica como la b-oxidación. En esta revisión, se discuten aspectos relacionados con el metabolismo de estos compuestos y su posible influencia en la producción de proteínas recombinantes, específicamente la Iduronato 2- sulfato sulfatasa humana (IDSh)