Hypocrates is a genetically encoded fluorescent biosensor for (pseudo)hypohalous acids and their derivatives

The lack of tools to monitor the dynamics of (pseudo)hypohalous acids in live cells and tissues hinders a better understanding of inflammatory processes. Here we present a fluorescent genetically encoded biosensor, Hypocrates, for the visualization of (pseudo)hypohalous acids and their derivatives. Hypocrates consists of a circularly permuted yellow fluorescent protein integrated into the structure of the transcription repressor NemR from Escherichia coli. We show that Hypocrates is ratiometric, reversible, and responds to its analytes in the 106 M-1s-1 range. Solving the Hypocrates X-ray structure provided insights into its sensing mechanism, allowing determination of the spatial organization in this circularly permuted fluorescent protein-based redox probe. We exemplify its applicability by imaging hypohalous stress in bacteria phagocytosed by primary neutrophils. Finally, we demonstrate that Hypocrates can be utilized in combination with HyPerRed for the simultaneous visualization of (pseudo)hypohalous acids and hydrogen peroxide dynamics in a zebrafish tail fin injury model

Measurement of Isolated Photons Accompanied by Jets in Deep Inelasic epep Scattering

The production of isolated high-energy photons accompanied by jets has been measured in deep inelastic ep scattering with the ZEUS detector at HERA, using an integrated luminosity of 326 pb^{-1}. Measurements were made for exchanged photon virtualities, Q^2, in the range 10 to 350 GeV^2. The photons were measured in the transverse-energy and pseudorapidity ranges 4 < ET^gamma < 15 GeV and -0.7 < eta^gamma < 0.9, and the jets were measured in the transverse-energy and pseudorapidity ranges 2.5 < ET^jet <35 GeV and -1.5 < eta^jet < 1.8. Differential cross sections are presented as functions of these quantities. Perturbative QCD predictions give a reasonable description of the shape of the measured cross sections over most of the kinematic range, but the absolute normalisation is typically in disagreement by 20-30%.Comment: 17 pages, 4 figures. Minor changes in published versio