Synthesis and Catalase Mimic Activity of MnO2 Nano Powder Prepared by Hydrothermal Process

حضرثنائي اوكسيد المنغنيز النانوي بطريقة الضغط الحراري (الاوتوكليف). وتم تلدين ثنائي اوكسيد المنغنيز عند درجات حرارية مختلفة (250،400،550 و700م˚). اخذت القياسات للمساحيق النانوية ولمتغيرات متعددة ومن ثم شخصت البنية التركيبية وطوغرافية الاسطح بوساطة فحص حيود الاشعة السينيه ((XRD, مجهر القوه الذريه ((AFM و المجهر الاكتروني الماسح ((SEM. درست فعالية ثنائي اوكسيد المنغنيز كعامل مقلد لانزيم الكتليز (الفعالية التحفيزية) ضد بيروكسيد الهيدروجين وباستخدام طريقة جديدة ووجد ان التلدين بدرجة حرارة 400˚م هي الافضل.Manganese dioxide (MnO2) nanopowder has been synthesized by hydrothermal method. MnO2 was annealed at different temperatures (250, 400, 550, 700˚C). The crystal structure and surface morphology of these nanostructures were characterized by X-ray diffraction (XRD), Atomic Force Microscope (AFM) and Scanning Electron Microscopy (SEM). The catalase mimic activity (catalytic activity) of MnO2 against hydrogen peroxide (H2O2) was studied by using the new method and found that 400˚C is the best annealing temperature

Correlation of Total Cholesterol and Glucose in Serum of Iraqi Patients with Atherosclerosis and Diabetes Mellitus Type 2

Cholesterol is a fatty substance (lipid) classified as a waxy steroid of fat. It is absorbed by the intestine into blood stream and is packaged inside a protein coat a chylomicron. Blood glucose is a simple monosaccharide absorbed directly into the blood stream during digestion. The level of blood glucose normally represents a balance between the inflow of glucose into blood and it is uptake by the tissue. Atherosclerosis is a general term for a number of different medical conditions that affect the heart, this is occurs when the blood supply to a part of heart is interrupted, must commonly due to plaque, is build up in the coronary arteries consist of lipid cholesterol and calcium. It causes a damage of potential disease of heart muscle due to thickening and hardening of arteries. Diabetes mellitus is a disorder in which the level of blood glucose is persistently elevated above the normal range due to decrease secretion of insulin. The main objective of this study is to determine the concentrations of total cholesterol and glucose into blood serum of Iraq patients with atherosclerosis and diabetes mellitus type 2. This study included 60 specimens of patients with atherosclerosis and 60 patients with type 2 diabetes mellitus; their age range was 45-65 years. These patients were then matched by age and sex to 30 healthy individuals. Results revealed that there was highly significantly increased in the mean value of total cholesterol and glucose concentrations in patients of atherosclerosis (p<0.001) and diabetes mellitus (p<0.001) as compared with healthy individuals

Impact of opioid-free analgesia on pain severity and patient satisfaction after discharge from surgery: multispecialty, prospective cohort study in 25 countries

Background: Balancing opioid stewardship and the need for adequate analgesia following discharge after surgery is challenging. This study aimed to compare the outcomes for patients discharged with opioid versus opioid-free analgesia after common surgical procedures.Methods: This international, multicentre, prospective cohort study collected data from patients undergoing common acute and elective general surgical, urological, gynaecological, and orthopaedic procedures. The primary outcomes were patient-reported time in severe pain measured on a numerical analogue scale from 0 to 100% and patient-reported satisfaction with pain relief during the first week following discharge. Data were collected by in-hospital chart review and patient telephone interview 1 week after discharge.Results: The study recruited 4273 patients from 144 centres in 25 countries; 1311 patients (30.7%) were prescribed opioid analgesia at discharge. Patients reported being in severe pain for 10 (i.q.r. 1-30)% of the first week after discharge and rated satisfaction with analgesia as 90 (i.q.r. 80-100) of 100. After adjustment for confounders, opioid analgesia on discharge was independently associated with increased pain severity (risk ratio 1.52, 95% c.i. 1.31 to 1.76; P &lt; 0.001) and re-presentation to healthcare providers owing to side-effects of medication (OR 2.38, 95% c.i. 1.36 to 4.17; P = 0.004), but not with satisfaction with analgesia (beta coefficient 0.92, 95% c.i. -1.52 to 3.36; P = 0.468) compared with opioid-free analgesia. Although opioid prescribing varied greatly between high-income and low- and middle-income countries, patient-reported outcomes did not.Conclusion: Opioid analgesia prescription on surgical discharge is associated with a higher risk of re-presentation owing to side-effects of medication and increased patient-reported pain, but not with changes in patient-reported satisfaction. Opioid-free discharge analgesia should be adopted routinely

STUDYING OF LIPID PEROXIDATION , LIPID PROFILE AND ATHEROSCLEROSIS INDEX LEVELS IN SMOKER IN RAMADI CITY .

The Study was carried out on a sample consisting of (60) persons ; (40) of them are smokers and (20) are non-smokers which considered as control group . It tries to explore the effect of smoking on the level of lipid per oxidation through determining MDA in the serum . Also , the effect of smoking on the lipid profile in serum and the possibility that smokers might be infected with atherosclerosis were study by determining Atherosclerosis index in the study sample . Results have shown a significant increase in the level of lipid per oxidation MDA (4.2μmol/L) in smokers in comparison with the non-smokers (2.1μmol/L) at p significant increase of both cholesterol , triglyceride , low density lipoprotein LDL and very low density lipoprotein VLDL in smoker which were (278mg/100ml) , ( 170 mg/100ml) , ( 204 mg/100ml) ,and (34 mg/100ml) respectively in comparison with the non-smoker which were (193 mg/100ml) , (114 mg/100ml) , ( 114 mg/100ml) and (22 mg/100ml) respectively of p other hand there has been a significant decrease of high density lipoprotein HDL in smoker (36 mg/100ml) in comparison with the non-smoker (57 mg/100ml) at p of atherosclerosis index we found that it significantly increase in smokers (7.7) in comparison with non-smoker (3.3) at p<0.05

Neutral sphingomyelinase 2 regulates inflammatory responses in monocytes/macrophages induced by TNF-α

Obesity is associated with elevated levels of TNF-alpha and proinflammatory CD11c monocytes/macrophages. TNF-alpha mediated dysregulation in the plasticity of monocytes/macrophages is concomitant with pathogenesis of several inflammatory diseases, including metabolic syndrome, but the underlying mechanisms are incompletely understood. Since neutral sphingomyelinase-2 (nSMase2: SMPD3) is a key enzyme for ceramide production involved in inflammation, we investigated whether nSMase2 contributed to the inflammatory changes in the monocytes/macrophages induced by TNF-alpha. In this study, we demonstrate that the disruption of nSMase activity in monocytes/macrophages either by chemical inhibitor GW4869 or small interfering RNA (siRNA) against SMPD3 results in defects in the TNF-alpha mediated expression of CD11c. Furthermore, blockage of nSMase in monocytes/macrophages inhibited the secretion of inflammatory mediators IL-1 beta and MCP-1. In contrast, inhibition of acid SMase (aSMase) activity did not attenuate CD11c expression or secretion of IL-1 beta and MCP-1. TNF-alpha-induced phosphorylation of JNK, p38 and NF-kappa B was also attenuated by the inhibition of nSMase2. Moreover, NF-kB/AP-1 activity was blocked by the inhibition of nSMase2. SMPD3 was elevated in PBMCs from obese individuals and positively corelated with TNF-alpha gene expression. These findings indicate that nSMase2 acts, at least in part, as a master switch in the TNF-alpha mediated inflammatory responses in monocytes/macrophages.Open access journalThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at [email protected]

ACSL1 is a key regulator of inflammatory and macrophage foaming induced by short-term palmitate exposure or acute high-fat feeding

Summary: Foamy and inflammatory macrophages play pathogenic roles in metabolic disorders. However, the mechanisms that promote foamy and inflammatory macrophage phenotypes under acute-high-fat feeding (AHFF) remain elusive. Herein, we investigated the role of acyl-CoA synthetase-1 (ACSL1) in favoring the foamy/inflammatory phenotype of monocytes/macrophages upon short-term exposure to palmitate or AHFF. Palmitate exposure induced a foamy/inflammatory phenotype in macrophages which was associated with increased ACSL1 expression. Inhibition/knockdown of ACSL1 in macrophages suppressed the foamy/inflammatory phenotype through the inhibition of the CD36-FABP4-p38-PPARδ signaling axis. ACSL1 inhibition/knockdown suppressed macrophage foaming/inflammation after palmitate stimulation by downregulating the FABP4 expression. Similar results were obtained using primary human monocytes. As expected, oral administration of ACSL1 inhibitor triacsin-C in mice before AHFF normalized the inflammatory/foamy phenotype of the circulatory monocytes by suppressing FABP4 expression. Our results reveal that targeting ACSL1 leads to the attenuation of the CD36-FABP4-p38-PPARδ signaling axis, providing a therapeutic strategy to prevent the AHFF-induced macrophage foaming and inflammation