Sequencing of E2 and NS5A regions of HCV genotype 3a in Brazilian patients with chronic hepatitis

Hepatitis C virus (HCV) is a major cause of liver disease throughout the world. The NS5A and E2 proteins of HCV genotype 1 were reported to inhibit the double-stranded (ds) RNA-dependent protein kinase (PKR), which is involved in the cellular antiviral response induced by interferon (IFN). The response to IFN therapy is quite different between genotypes, with response rates among patients infected with types 2 and 3 that are two-three-fold higher than in patients infected with type 1. Interestingly, a significant percentage of HCV genotype 3-infected patients do not respond to treatment at all. The aim of this paper was to analyse the sequences of fragments of the E2 and NS5A regions from 33 outpatients infected with genotype 3a, including patients that have responded (SVR) or not responded (NR) to treatment. HCV RNA was extracted and amplified with specific primers for the NS5A and E2 regions and the PCR products were then sequenced. The sequences obtained covered amino acids (aa) 636-708 in E2 and in NS5A [including the IFN sensitivity determining region (ISDR), PKR-binding domain and extended V3 region)]. In the E2 and NS5A regions, we did observe aa changes among patients, but these changes were not statistically significant between the SVR and NR groups. In conclusion, our results suggest that the ISDR domain is not predictive of treatment success in patients infected with HCV genotype 3a.publishersversionpublishe

Computer analysis of objects’ movement in image sequences: methods and applications

Computer analysis of objects’ movement in image sequences is a very complex problem, considering that it usually involves tasks for automatic detection, matching, tracking, motion analysis and deformation estimation. In spite of its complexity, this computational analysis has a wide range of important applications; for instance, in surveillance systems, clinical analysis of human gait, objects recognition, pose estimation and deformation analysis. Due to the extent of the purposes, several difficulties arise, such as the simultaneous tracking of manifold objects, their possible temporary occlusion or definitive disappearance from the image scene, changes of the viewpoints considered in images acquisition or of the illumination conditions, or even nonrigid deformations that objects may suffer in image sequences. In this paper, we present an overview of several methods that may be considered to analyze objects’ movement; namely, for their segmentation, tracking and matching in images, and for estimation of the deformation involved between images.This paper was partially done in the scope of project “Segmentation, Tracking and Motion Analysis of Deformable (2D/3D) Objects using Physical Principles”, with reference POSC/EEA-SRI/55386/2004, financially supported by FCT -Fundação para a Ciência e a Tecnologia from Portugal. The fourth, fifth and seventh authors would like to thank also the support of their PhD grants from FCT with references SFRH/BD/29012/2006, SFRH/BD/28817/2006 and SFRH/BD/12834/2003, respectively

Variabilidade Do Desempenho No Line-drill Test Em Adolescentes Jogadores De Basquetebol

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)The practice of basketball during adolescence increases the body size and functional performance. Basketball involves short-time maximum effort for changes of direction, which use anaerobic metabolism and agility. Objective: To examine the variation in performance in the Line-drill Test in adolescent basketball players (n=59, age 9-15 years) in relation to the stage of somatic maturity (estimated age of peak growth velocity [PGV]) and the variation in body size. Methods: We considered chronological age, estimated time for the age of PGV by the maturity offset protocol, height and body mass measured by anthropometry, and performance in the Line Drill Test. Proportional allometric models were used to control the variation associated with the body size, chronological age, and maturity of inter-individual variation in the Line-drill Test. Results: We found a high negative linear relationship between performance in the Line-drill Test and chronological age (r=-0.64, 95% IC -0.77 - -0.46, p<0.01). The relationship between performance on the Line-drill Test and the time until the age of PGV proved to be non-linear, indicating slowdown in performance variation, approximately from the age of PGV and one year after the predicted PGV. The allometric models showed a negative relationship between body size and performance in the Line-drill Test. The amplitude of variance explained in the proportional allometric models ranged from 0.28 to 0.48. Negative exponents of small magnitude were observed for chronological age and the indicator of maturity being, however, significant and with reduced amplitude (-0.05 to -0.06) Conclusion: The use of allometric models to control the influence of body size can potentially help to understand the development of agility performances in adolescent basketball players. © 2016, Redprint Editora Ltda. All rights reserved.226445449PNPD/CAPES/2013, CAPES, Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorFAPESP, Fundação de Amparo à Pesquisa do Estado de São PauloCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP

Prevalence and risk factors for bovine leptospirosis in Mato Grosso do Sul, Brazil

Foi investigada a prevalência de anticorpos antileptospira em fêmeas bovinas com idade igual ou superior a 24 meses, provenientes de 178 rebanhos de 22 municípios do estado de Mato Grosso do Sul, bem como identificados fatores de risco associados à infecção. Foram analisadas 2.573 amostras de soro sangüíneo por meio do teste de soroaglutinação microscópica perante 10 sorovares de leptospira. Títulos iguais ou superiores a 100 para um ou mais sorovares foram detectados em 1.801 fêmeas (98,8%) de 161 (96,5%) rebanhos. O sorovar Hardjo (65,6%) foi apontado como o mais provável, seguido do sorovar Wolffi (12,3%). Os resultados demonstram que a leptospirose bovina se encontra presente em todos os municípios estudados, com alta prevalência, tanto em animais como em rebanhos. Os fatores de risco identificados neste estudo e associados à infecção por bactérias do gênero lepstopira foram o tipo de exploração pecuária de corte e a raça Zebu. _____________________________________________________________________________________________________________ ABSTRACTThe prevalence of anti-Leptospira spp. antibodies was estimated for female cattle aged 24 months or older. The sample comprised 178 herds from 22 counties in the state of Mato Grosso do Sul, Brazil. The risk factors associated with the presence of infeccion were investigated. A total of 2,573 blood serum samples were tested against 10 leptospira serovars using the microagglutination test (MAT). Titers of 100 or higher for one or more serovars were detected in 1,801 females (98.8%) from 161 herds (96.5%). Serovar Hardjo (65.6%) was the most frequent, followed by serovar Wolffi (12.3%). These results suggest that bovine leptospirosis is widespread in all the counties under study, with a high prevalence both at the animal and the herd level. Beef farms and the Zebu breed were associated to the higher risk of herd infection by leptospiras