miRNAs regulate SIRT1 expression during mouse embryonic stem cell differentiation and in adult mouse tissues

SIRT1 is increasingly recognized as a critical regulator of stress responses, replicative senescence, inflammation, metabolism, and aging. SIRT1 expression is regulated transcriptionally and post-transcriptionally, and its enzymatic activity is controlled by NAD+ levels and interacting proteins. We found that SIRT1 protein levels were much higher in mouse embryonic stem cells (mESCs) than in differentiated tissues. miRNAs post-transcriptionally downregulated SIRT1 during mESC differentiation and maintained low levels of SIRT1 expression in differentiated tissues. Specifically, miR-181a and b, miR-9, miR-204, miR-199b, and miR-135a suppressed SIRT1 protein expression. Inhibition of mir-9, the SIRT1-targeting miRNA induced earliest during mESC differentiation, prevented SIRT1 downregulation. Conversely, SIRT1 protein levels were upregulated post-transcriptionally during the reprogramming of mouse embryonic fibroblasts (MEFs) into induced pluripotent stem (iPS) cells. The regulation of SIRT1 protein levels by miRNAs might provide new opportunities for therapeutic tissue-specific modulation of SIRT1 expression and for reprogramming of somatic cells into iPS cells

Tohoku-Hiroshima-Nagoya planetary spectra library: A method for characterizing planets in the visible to near infrared

There has not been a comprehensive framework for comparing spectral data from different planets.Such a framework is needed for the study of extrasolar planets and objects within the solar system. We have undertaken observations to compile a library of planet spectra for all planets, some moons, and some dwarf planets in the solar system to study their general spectroscopic and photometric natures. During May and November of 2008, we acquired spectra for the planets using TRISPEC, which is capable of simultaneous three-band spectroscopy in a wide wavelength range of 0.45 - 2.5 microns with low resolving power (lambda-over-Delta-lambda is 140 - 360). Patterns emerge from comparing the spectra. Analyzing their general spectroscopic and photometric natures, we show that it is possible to distinguish between gas planets, soil planets and ice planets. These methods can be applied to extrasolar observations using low resolution spectrography or broad-band filters. The present planet spectral library is the first library to contain observational spectra for all of the solar system planets, based on simultaneous observations in visible and near infrared wavelengths. This library will be a useful reference for analyzing extrasolar planet spectra, and for calibrating planetary data sets.Comment: 11 pages, 6 figures, Accepted on 28/08/2009 to appear in Section 10. Planets and planetary systems of Astronomy and Astrophysic

MITSuME--Multicolor Imaging Telescopes for Survey and Monstrous Explosions

Development of MITSuME is reported. Two 50-cm optical telescopes have been built at Akeno in Yamanashi prefecture and at Okayama Astrophysical Observatory (OAO) in Okayama prefecture. Three CCD cameras for simultaneous g'RcIc photometry are to be mounted on each focal plane, covering a wide FOV of about 30" x 30". The limiting magnitude at V is fainter than 18. In addition to these two optical telescopes, a 91-cm IR telescope with a 1 deg x 1 deg field of view is being built at OAO, which performs photometry in YJHK bands. These robotic telescopes can start the observation of counterparts of a GRB within a minute from an alert. We aim to obtain photometric redshifts exceeding 10 with these telescopes. The performance and the current construction status of the telescopes are presented.Comment: 4 pages, 3 figures, 4th Workshop on Gamma-Ray Burst in the Afterglow Era, Roma, October 18-22, 200

Heat Treatment Inhibits Skeletal Muscle Atrophy of Glucocorticoid- Induced Myopathy in Rats

Summary The purpose of this study was to investigate the influence of heat treatment on glucocorticoid (GC)-induced myopathy. Eight-weekold Wistar rats were randomly assigned to the control, Dex, and Dex + Heat groups. Dexamethasone (2 mg/kg) was injected subcutaneously 6 days per week for 2 weeks in the Dex and Dex + Heat group. In the Dex + Heat group, heat treatment was performed by immersing hindlimbs in water at 42 °C for 60 min, once every 3 days for 2 weeks. The extensor digitorum longus muscle was extracted following 2 weeks of experimentation. In the Dex + Heat group, muscle fiber diameter, capillary/muscle fiber ratio, and level of heat shock protein 72 were significantly higher and atrogene expression levels were significantly lower than in the Dex group. Our results suggest that heat treatment inhibits the development of GC-induced myopathy by decreasing atrogene expression and increasing angiogenesis

Optical Spectropolarimetry of SN 2002ap: High Velocity Asymmetric Explosion

We present spectropolarimetry of the Type Ic supernova SN 2002ap and give a preliminary analysis: the data were taken at two epochs, close to and one month later than the visual maximum (2002 February 8). In addition we present June 9 spectropolarimetry without analysis. The data show the development of linear polarization. Distinct polarization profiles were seen only in the O I \lambda 7773 multiplet/Ca II IR triplet absorption trough at maximum light and in the Ca II IR triplet absorption trough a month later, with the latter showing a peak polarization as high as ~2 %. The intrinsic polarization shows three clear position angles: 80 degs for the February continuum, 120 degs for the February line feature, and 150 degs for the March data. We conclude that there are multiple asymmetric components in the ejecta. We suggest that the supernova has a bulk asymmetry with an axial ratio projected on the sky that is different from 1 by of order 10 %. Furthermore, we suggest very speculatively that a high velocity ejecta component moving faster than ~0.115c (e.g., a jet) contributes to polarization in the February epoch.Comment: 7 pages, 3 figures, accepted for publication in the Astrophysical Journal (Letters

Homogeneous bubble nucleation limit of mercury under the normal working conditions of the planned European Spallation Source

In spallation neutron sources, liquid mercury is the subject of big thermal and pressure shocks, upon adsorbing the proton beam. These changes can cause unstable bubbles in the liquid, which can damage the structural material. While there are methods to deal with the pressure shock, the local temperature shock cannot be avoided. In our paper we calculated the work of the critical cluster formation (i.e. for mercury micro-bubbles) together with the rate of their formation (nucleation rate). It is shown that the homogeneous nucleation rates are very low even after adsorbing several proton pulses, therefore the probability of temperature induced homogeneous bubble nucleation is negligible.Comment: 22 Pages, 11 figures, one of them is colour, we plan to publish it in Eur. Phys. J.

B1 SINE-binding ZFP266 impedes mouse iPSC generation through suppression of chromatin opening mediated by reprogramming factors

Induced pluripotent stem cell (iPSC) reprogramming is inefficient and understanding the molecular mechanisms underlying this inefficiency holds the key to successfully control cellular identity. Here, we report 24 reprogramming roadblock genes identified by CRISPR/Cas9-mediated genome-wide knockout (KO) screening. Of these, depletion of the predicted KRAB zinc finger protein (KRAB-ZFP) Zfp266 strongly and consistently enhances murine iPSC generation in several reprogramming settings, emerging as the most robust roadblock. We show that ZFP266 binds Short Interspersed Nuclear Elements (SINEs) adjacent to binding sites of pioneering factors, OCT4 (POU5F1), SOX2, and KLF4, and impedes chromatin opening. Replacing the KRAB co-suppressor with co-activator domains converts ZFP266 from an inhibitor to a potent facilitator of iPSC reprogramming. We propose that the SINE-KRAB-ZFP interaction is a critical regulator of chromatin accessibility at regulatory elements required for efficient cellular identity changes. In addition, this work serves as a resource to further illuminate molecular mechanisms hindering reprogramming.Induced pluripotent stem cell (iPSC) reprogramming is inherently inefficient. Here the authors identify 24 reprogramming roadblock genes through a CRISPR/Cas9-mediated genome-wide knockout screen including a KRAB-ZFP Zfp266, knockout of which consistently enhances murine iPSC generation.Peer reviewe