Volatile profiling distinguishes Streptococcus pyogenes from other respiratory streptococcal species

Sore throat is one of the most common complaints encountered in the ambulatory clinical setting. Rapid, culture-independent diagnostic techniques that do not rely on pharyngeal swabs would be highly valuable as a point-of-care strategy to guide outpatient antibiotic treatment. Despite the promise of this approach, efforts to detect volatiles during oropharyngeal infection have yet been limited. In our research study, we sought to evaluate for specific bacterial volatile organic compounds (VOC) biomarkers in isolated culture

Variation in the length and structure of reports written by reporting radiographers: A retrospective study.

IntroductionThe literature suggests that there is variation in various features of the written radiology report for a range of body areas and imaging modalities. The retrospective study presented here aims to determine if similar variation is demonstrated in a group of 5 reporting radiographers in a UK NHS Trust.MethodsFull reports for 1530 knee radiographic examinations performed from accident and emergency referrals were extracted for a 12-month period from a Radiology Information System (RIS) into Excel. Copied into Word, the word count function was used for each report and the number of words and characters (without spaces) was returned into Excel. Average word count and word length per report, by radiographer, were calculated for the following sections of the report: report title, main body and signature. SPSS was used to perform inferential statistical analysis.ResultsA wide range in the maximum and minimum average report lengths (60.88 v 17.83 words) was demonstrated. Statistically significant differences (p ConclusionVariation in report structure and length, as well as word length, was seen, comparable to studies of radiologist reports. Further research is required to investigate the drivers of this variation, and determine if there is any clinical significance

Defining the Realized Niche of the Two Major Clades of \u3ci\u3eTrichodesmium\u3c/i\u3e: A Study on the West Florida Shelf

The cyanobacterium Trichodesmium plays an essential role supporting ocean productivity by relieving nitrogen limitation via dinitrogen (N-2) fixation. The two common Trichodesmium clades, T. erythraeum and T. thiebautii, are both observed in waters along the West Florida Shelf (WFS). We hypothesized that these taxa occupy distinct realized niches, where T. thiebautii is the more oceanic clade. Samples for DNA and water chemistry analyses were collected on three separate WFS expeditions (2015, 2018, and 2019) spanning multiple seasons; abundances of the single copy housekeeping gene rnpB from both clades were enumerated via quantitative PCR. We conducted a suite of statistical analyses to assess Trichodesmium clade abundances in the context of the physicochemical data. We observed a consistent coastal vs. open ocean separation of the two clades: T. erythraeum was found in shallow waters where the concentrations of dissolved iron (dFe) and the groundwater tracer Ba were significantly higher, while T. thiebautii abundance was positively correlated with water column depth. The Loop Current intrusion in 2015 with entrained Missisippi River water brought higher dFe and elevated abundance of both clades offshore of the 50 m isobath, suggesting that both clades are subject to Fe limitation on the outer shelf. Whereas, previous work has observed that T. thiebautii is more abundant than T. erythraeum in open ocean surface waters, this is the first study to examine Trichodesmium niche differentiation in a coastal environment. Understanding the environmental niches of these two key taxa bears important implications for their contributions to global nitrogen and carbon cycling and their response to global climate change

Ice sheet and palaeoclimate controls on drainage network evolution: an example from Dogger Bank, North Sea

Submerged landscapes on continental shelves archive drainage networks formed during periods of sea-level lowstand. The evolution of these postglacial drainage networks also reveals how past climate changes affected the landscape. Ice-marginal and paraglacial drainage networks on low-relief topography are susceptible to reorganisation of water supply, forced by ice-marginal rearrangement, precipitation and temperature variations, and marine inundation. A rare geological archive of climate-driven landscape evolution during the transition from ice-marginal (ca. 23 ka) to a fully submerged marine environment (ca. 8 ka) is preserved at Dogger Bank, in the southern North Sea. In this study, our analysis of high-resolution seismic reflection and cone penetration test data reveal a channel network over a 1330 km2 area that incised glacial and proglacial lake-fill sediments. The channel network sits below coastal and shallow marine sediments and is therefore interpreted to represent a terrestrial drainage network. When mapped out, the channel form morphology reveals two distinct sets. The first set comprises two low-sinuosity, wide (>400 m) channels that contain macroforms of braid and side bars. These channels are interpreted to have originated as proglacial rivers, which drained the ice-sheet margin to the north. The second set of channels (75–200 m wide, with one larger, ∼400 m wide) has higher sinuosity and forms a subdendritic network of tributaries to the proglacial channels. The timing of channel formation lacks chronostratigraphic control. However, the proglacial rivers must have formed as the ice sheet was still on Dogger Bank, before 23 ka, to supply meltwater to the rivers. Ice-sheet retreat from Dogger Bank led to reorganisation of meltwater drainage and abandonment of the proglacial rivers. Palaeoclimate simulations show a cold and dry period at Dogger Bank between 23 and 17 ka. After 17 ka, precipitation increased, and drainage of precipitation formed the second set of channels. The second set of rivers remained active until marine transgression of Dogger Bank at ca. 8.5–8 ka. Overall, this study provides a detailed insight into the evolution of river networks across Dogger Bank and highlights the interplay between external (climate) and internal (local) forcings in drainage network evolution

Phased occupation and retreat of the last British–Irish Ice Sheet in the southern North Sea: geomorphic and seismostratigraphic evidence of a dynamic ice lobe

Along the terrestrial margin of the southern North Sea, previous studies of the MIS 2 glaciation impacting eastern Britain have played a significant role in the development of principles relating to ice sheet dynamics (e.g. deformable beds), and the practice of reconstructing the style, timing, and spatial configuration of palaeo-ice sheets. These detailed terrestrially-based findings have however relied on observations made from only the outer edges of the former ice mass, as the North Sea Lobe (NSL) of the British-Irish Ice Sheet (BIIS) occupied an area that is now almost entirely submarine (c.21–15 ka). Compounded by the fact that marine-acquired data have been primarily of insufficient quality and density, the configuration and behaviour of the last BIIS in the southern North Sea remains surprisingly poorly constrained. This paper presents analysis of a new, integrated set of extensive seabed geomorphological and seismo-stratigraphic observations that both advances the principles developed previously onshore (e.g. multiple advance and retreat cycles), and provides a more detailed and accurate reconstruction of the BIIS at its southern-most extent in the North Sea. A new bathymetry compilation of the region reveals a series of broad sedimentary wedges and associated moraines that represent several terminal positions of the NSL. These former still-stand ice margins (1–4) are also found to relate to newly-identified architectural patterns (shallow stacked sedimentary wedges) in the region's seismic stratigraphy (previously mapped singularly as the Bolders Bank Formation). With ground-truthing constraint provided by sediment cores, these wedges are interpreted as sub-marginal till wedges, formed by complex subglacial accretionary processes that resulted in till thickening towards the former ice-sheet margins. The newly sub-divided shallow seismic stratigraphy (at least five units) also provides an indication of the relative event chronology of the NSL. While there is a general record of south-to-north retreat, seismic data also indicate episodes of ice-sheet re-advance suggestive of an oscillating margin (e.g. MIS 2 maximum not related to first incursion of ice into region). Demonstrating further landform interdependence, geographically-grouped sets of tunnel valleys are shown to be genetically related to these individual ice margins, providing clear insight into how meltwater drainage was organised at the evolving termini of this dynamic ice lobe. The newly reconstructed offshore ice margins are found to be well correlated with previously observed terrestrial limits in Lincolnshire and E. Yorkshire (Holderness) (e.g. MIS 2 maximum and Withernsea Till). This reconstruction will hopefully provide a useful framework for studies targeting the climatic, mass-balance, and external glaciological factors (i.e. Fennoscandian Ice Sheet) that influenced late-stage advance and deglaciation, important for accurately characterising both modern and palaeo-ice sheets

CARD14 Gain-of-Function Mutation Alone Is Sufficient to Drive IL-23/IL-17-Mediated Psoriasiform Skin Inflammation In Vivo.

Rare autosomal dominant mutations in the gene encoding the keratinocyte signaling molecule CARD14, have been associated with an increased susceptibility to psoriasis, but the physiological impact of CARD14 gain-of-function mutations remains to be fully determined in vivo. Here, we report that heterozygous mice harboring a CARD14 gain-of-function mutation (Card14ΔE138) spontaneously develop a chronic psoriatic phenotype with characteristic scaling skin lesions, epidermal thickening, keratinocyte hyperproliferation, hyperkeratosis, and immune cell infiltration. Affected skin of these mice is characterized by elevated expression of anti-microbial peptides, chemokines, and cytokines (including T helper type 17 cell-signature cytokines) and an immune infiltrate rich in neutrophils, myeloid cells, and T cells, reminiscent of human psoriatic skin. Disease pathogenesis was driven by the IL-23/IL-17 axis, and neutralization of IL-23p19, the key cytokine in maintaining T helper type 17 cell polarization, significantly reduced skin lesions and the expression of antimicrobial peptides and proinflammatory cytokines. Therefore, hyperactivation of CARD14 alone is sufficient to orchestrate the complex immunopathogenesis that drives T helper type 17-mediated psoriasis skin disease in vivo

Acute cardiometabolic effects of brief active breaks in sitting for patients with rheumatoid arthritis

Exercise is a treatment in rheumatoid arthritis, but participation in moderate-to-vigorous exercise is challenging for some patients. Light-intensity breaks in sitting could be a promising alternative. We compared the acute effects of active breaks in sitting with those of moderate-to-vigorous exercise on cardiometabolic risk markers in patients with rheumatoid arthritis. In a crossover fashion, 15 women with rheumatoid arthritis underwent three 8-h experimental conditions: prolonged sitting (SIT), 30-min bout of moderate-to-vigorous exercise followed by prolonged sitting (EX), and 3-min bouts of light-intensity walking every 30 min of sitting (BR). Postprandial glucose, insulin, c-peptide, triglycerides, cytokines, lipid classes/subclasses (lipidomics), and blood pressure responses were assessed. Muscle biopsies were collected following each session to assess targeted proteins/genes. Glucose [−28% in area under the curve (AUC), P = 0.036], insulin (−28% in AUC, P = 0.016), and c-peptide (−27% in AUC, P = 0.006) postprandial responses were attenuated in BR versus SIT, whereas only c-peptide was lower in EX versus SIT (−20% in AUC, P = 0.002). IL-1β decreased during BR, but increased during EX and SIT (P = 0.027 and P = 0.085, respectively). IL-1ra was increased during EX versus BR (P = 0.002). TNF-α concentrations decreased during BR versus EX (P = 0.022). EX, but not BR, reduced systolic blood pressure (P = 0.013). Lipidomic analysis showed that 7 of 36 lipid classes/subclasses were significantly different between conditions, with greater changes being observed in EX. No differences were observed for protein/gene expression. Brief active breaks in sitting can offset markers of cardiometabolic disturbance, which may be particularly useful for patients who may find it difficult to adhere to exercise. NEW & NOTEWORTHY Exercise is a treatment in rheumatoid arthritis but is challenging for some patients. Light-intensity breaks in sitting could be a promising alternative. Our findings show beneficial, but differential, cardiometabolic effects of active breaks in sitting and exercise in patients with rheumatoid arthritis. Breaks in sitting mainly improved glycemic and inflammatory markers, whereas exercise improved lipidomic and hypotensive responses. Breaks in sitting show promise in offsetting aspects of cardiometabolic disturbance associated with prolonged sitting in rheumatoid arthritis

Drilling-induced and logging-related features illustrated from IODP-ICDP Expedition 364 downhole logs and borehole imaging tools

Expedition 364 was a joint IODP and ICDP mission-specific platform (MSP) expedition to explore the Chicxulub impact crater buried below the surface of the Yucatán continental shelf seafloor. In April and May 2016, this expedition drilled a single borehole at Site M0077 into the crater's peak ring. Excellent quality cores were recovered from ~ 505 to ~1335m below seafloor (m b.s.f.), and high-resolution open hole logs were acquired between the surface and total drill depth. Downhole logs are used to image the borehole wall, measure the physical properties of rocks that surround the borehole, and assess borehole quality during drilling and coring operations. When making geological interpretations of downhole logs, it is essential to be able to distinguish between features that are geological and those that are operation-related. During Expedition 364 some drilling-induced and logging-related features were observed and include the following: effects caused by the presence of casing and metal debris in the hole, logging-tool eccentering, drilling-induced corkscrew shape of the hole, possible re-magnetization of low-coercivity grains within sedimentary rocks, markings on the borehole wall, and drilling-induced changes in the borehole diameter and trajectory

Early paleocene paleoceanography and export productivity in the Chicxulub crater

The Chicxulub impact caused a crash in productivity in the world''s oceans which contributed to the extinction of ~75% of marine species. In the immediate aftermath of the extinction, export productivity was locally highly variable, with some sites, including the Chicxulub crater, recording elevated export production. The long-term transition back to more stable export productivity regimes has been poorly documented. Here, we present elemental abundances, foraminifer and calcareous nannoplankton assemblage counts, total organic carbon, and bulk carbonate carbon isotope data from the Chicxulub crater to reconstruct changes in export productivity during the first 3 Myr of the Paleocene. We show that export production was elevated for the first 320 kyr of the Paleocene, declined from 320 kyr to 1.2 Myr, and then remained low thereafter. A key interval in this long decline occurred 900 kyr to 1.2 Myr post impact, as calcareous nannoplankton assemblages began to diversify. This interval is associated with fluctuations in water column stratification and terrigenous flux, but these variables are uncorrelated to export productivity. Instead, we postulate that the turnover in the phytoplankton community from a post-extinction assemblage dominated by picoplankton (which promoted nutrient recycling in the euphotic zone) to a Paleocene pelagic community dominated by relatively larger primary producers like calcareous nannoplankton (which more efficiently removed nutrients from surface waters, leading to oligotrophy) is responsible for the decline in export production in the southern Gulf of Mexico. © 2021. American Geophysical Union. All Rights Reserved

Glucagon-like peptide-1 (GLP-1) and the regulation of human invariant natural killer T cells: lessons from obesity, diabetes and psoriasis

Aims/hypothesis The innate immune cells, invariant natural killer T cells (iNKT cells), are implicated in the pathogenesis of psoriasis, an inflammatory condition associated with obesity and other metabolic diseases, such as diabetes and dyslipidaemia. We observed an improvement in psoriasis severity in a patient within days of starting treatment with an incretin-mimetic, glucagon-like peptide-1 (GLP-1) receptor agonist. This was independent of change in glycaemic control. We proposed that this unexpected clinical outcome resulted from a direct effect of GLP-1 on iNKTcells. Methods We measured circulating and psoriatic plaque iNKT cell numbers in two patients with type 2 diabetes and psoriasis before and after commencing GLP-1 analogue therapy. In addition, we investigated the in vitro effects of GLP-1 on iNKT cells and looked for a functional GLP-1 receptor on these cells. Results The Psoriasis Area and Severity Index improved in both patients following 6 weeks of GLP-1 analogue therapy. This was associated with an alteration in iNKT cell number, with an increased number in the circulation and a decreased number in psoriatic plaques. The GLP-1 receptor was expressed on iNKT cells, and GLP-1 induced a dose-dependent inhibition of iNKT cell cytokine secretion, but not cytolytic degranulation in vitro. Conclusions/interpretation The clinical effect observed and the direct interaction between GLP-1 and the immune system raise the possibility of therapeutic applications for GLP-1 in inflammatory conditions such as psoriasis