Stable isotope compositions of fossil mollusks from southern California: Evidence for a cool last interglacial ocean

Stable isotope compositions have been determined for modem mullusks and fossil mollusks collected from uplied marine terraces at three l d t i e s in southern California. By using a paleocliatic model that decouples the temperature and ice-volume signals in ocean water, ocean-water temperatures off southern California are estimated to have been -3.8 °C at ~85 ka, -3.0 °C at ~107 ka, and -2.2 °C at ~125 ka relative to present temperature. These results indicate rather cool conditions during the peak of the last interglacial stage at 125 ka and conflict with results from terrace faunal studies that suggest water temperatures were warm or warmer than at present

Constraints on the timing of crustal imbrication in the central Trans-Hudson Orogen from single zircon 207Pb/206Pb ages of granitoid rocks from the Pelican thrust zone, Saskatchewan

The Pelican thrust is a major ductile high-strain zone in the Reindeer Zone, Trans-Hudson Orogen, northern Saskatchewan. It is interpreted as the main sole thrust separating stacked juvenile Paleoproterozoic allochthons and underlying Archean microcontinental crust in this central part of the orogen. Exposed nonmylonitic rocks in the footwall of the thrust consist of the Sahli monzocharnockite and the smaller, more highly retrograded MacMillan Point granite. Protomylonitic to ultramylonitic gneisses in the thrust zone derive from a variety of prethrust protoliths. A footwall "internal suite" mainly comprises quartzofeldspathic orthogneisses ("Q" gneisses) and high-grade migmatitic paragneisses. Hanging-wall "external suite" mylonitic gneisses include feldspar-porphyroclastic hornblendic grey gneisses probably derived from arc plutons, and laminated amphibolites derived from volcanic rocks. The overlying allochthon mainly comprises protoliths equivalent to those of the porphyroclastic orthogneisses and laminated amphibolites, together with interfolded and overlying Paleoproterozoic paragneisses of the Kisseynew domain. The Sahli monzocharnockite yields 207Pb/206Pb zircon and whole-rock Rb-Sr ages of ca. 2500 Ma, and the "Q" gneisses give 207Pb/206Pb zircon ages of up to ca. 2900 Ma, implying that most of the internal suite (footwall) mylonite protoliths are Archean. In contrast, external suite (hanging wall) porphyroclastic orthogneisses yield ca. 1880-1840 Ma 207Pb/206Pb zircon ages. Main, peak-metamorphic displacement on the Pelican thrust is interpreted to have occurred mainly between 1840 and 1820 Ma, as indicated by 207Pb/206Pb zircon ages from small, highly deformed synthrusting granite-pegmatite neosomal bodies in the thrust zone. Undeformed postcollisional granites and pegmatites were emplaced ∼1789 Ma. Total duration from arc development to completion of arc-continent collision was ∼100 Ma. The Pelican thrust zone may be similar in significance and style to younger, major, ocean closure related thrusts such as the Frontal Pennine thrust of the western Alps and the Main Mantle, Main Boundary, and Main Central thrusts of the Himalayas. As for the Pelican thrust, these displace oceanic rocks over older basement.published_or_final_versio

Semicontinuous Bioreactor Production of Recombinant Butyrylcholinesterase in Transgenic Rice Cell Suspension Cultures.

An active and tetrameric form of recombinant butyrylcholinesterase (BChE), a large and complex human enzyme, was produced via semicontinuous operation in a transgenic rice cell suspension culture. After transformation of rice callus and screening of transformants, the cultures were scaled up from culture flask to a lab scale bioreactor. The bioreactor was operated through two phases each of growth and expression. The cells were able to produce BChE during both expression phases, with a maximum yield of 1.6 mg BChE/L of culture during the second expression phase. Cells successfully regrew during a 5-day growth phase. A combination of activity assays and Western blot analysis indicated production of an active and fully assembled tetramer of BChE

Plant cell culture platforms for production of bioscavengers for biodefense

There is a critical need for flexible, rapid, cost effective biomanufacturing platforms for medical countermeasures. Our team has developed plant cell culture-based manufacturing platforms for production of recombinant protein bioscavengers against organophosphate (OP) nerve agents and anthrax toxins using both stable transgenic cell cultures for known chemical and biological threats, as well as transient production for rapid response to new and/or unanticipated threats. Plant cells offer several advantages over other hosts for production of medical countermeasures, particularly their ability to produce complex biologics and perform post-translational modification, inherent biosafety since they don\u27t harbor or propagate mammalian viruses thereby simplifying and/or eliminating viral clearance steps required for mammalian production systems. Plant cells are robust, have minimal nutrient requirements (grow in simple, chemically defined media containing sucrose, salts and plant hormones), and are relatively insensitive to changes in environmental conditions. These characteristics, robustness of upstream cultivation/use and reduced downstream purification requirements, make plant cells an ideal choice for field-deployable production of medical countermeasures. Here we present results for the production of functional recombinant butyrylcholinesterase (BChE), an OP nerve agent bioscavenger, in transgenic rice cell suspension cultures in different bioreactor configurations, and transient production of a bioscavenger against an anthrax toxin in N. benthamiana cell cultures. Techno-economic models for scaled-up versions of these plant cell culture production systems will also be presented

On the Distortion and Warpage of 7249 Aluminum Alloy After Quenching and Machining

The objective of this study is to determine the effect of solution treatment temperature, quenching media, and various machining sequences on the warpage behavior of aluminum 7249 alloy aged to T6 and T7\u27 tempers. Large extrusions of 7249 aluminum alloy with fins were cut into 108 T sections. The samples were solution-treated, aged, and machined. Three solution temperatures (445, 474, and 505 A degrees C), two quenching media (water and 20% polyalkylene glycol), two aging treatments (T6 and T7\u27), and three machine sequences were used. The flatness of the samples was measured on the surfaces orthogonal to the z-axis. Three points were on top of both shoulders (six total), six were at the bottom of the sample, and six were on the top of the fin, in the cases where the fin was not milled off. They were then averaged together by surface to represent the overall warpage of each sample

Stable isotopes can be used to infer the overwintering locations of prebreeding marine birds in the Canadian Arctic

Although assessments of winter carryover effects on fitness-related breeding parameters are vital for determining the links between environmental variation and fitness, direct methods of determining overwintering distributions (e.g., electronic tracking) can be expensive, limiting the number of individuals studied. Alternatively, stable isotope analysis in specific tissues can be used as an indirect means of determining individual overwintering areas of residency. Although increasingly used to infer the overwintering distributions of terrestrial birds, stable isotopes have been used less often to infer overwintering areas of marine birds. Using Arctic-breeding common eiders, we test the effectiveness of an integrated stable isotope approach (13-carbon, 15-nitrogen, and 2-hydrogen) to infer overwintering locations. Knowing the overwinter destinations of eiders from tracking studies at our study colony at East Bay Island, Nunavut, we sampled claw and blood tissues at two known overwintering locations, Nuuk, Greenland, and Newfoundland, Canada. These two locations yielded distinct tissue-specific isotopic profiles. We then compared the isotope profiles of tissues collected from eiders upon their arrival at our breeding colony, and used a k-means cluster analysis approach to match arriving eiders to an overwintering group. Samples from the claws of eiders were most effective for determining overwinter origin, due to this tissue\u27s slow growth rate relative to the 40-day turnover rate of blood. Despite taking an integrative approach using multiple isotopes, k-means cluster analysis was most effective when using 13-carbon alone to assign eiders to an overwintering group. Our research demonstrates that it is possible to use stable isotope analysis to assign an overwintering location to a marine bird. There are few examples of the effective use of this technique on a marine bird at this scale; we provide a framework for applying this technique to detect changes in the migration phenology of birds\u27 responses to rapid changes in the Arctic

Petrographical and geochemical evidences for paragenetic sequence interpretation of diagenesis in mixed siliciclastic–carbonate sediments: Mozduran Formation (Upper Jurassic), south of Agh-Darband, NE Iran

The Upper Jurassic Mozduran Formation with a thickness of 420 m at the type locality is the most important gas-bearing reservoir in NE Iran. It is mainly composed of limestone, dolostone with shale and gypsum interbeds that grade into coarser siliciclastics in the easternmost part of the basin. Eight stratigraphic sections were studied in detail in south of the Agh-Darband area. These analyses suggest that four carbonate facies associations and three siliciclastic lithofacies were deposited in shallow marine to shoreline environments, respectively. Cementation, compaction, dissolution, micritization, neomorphism, hematitization, dolomitization and fracturing are diagenetic processes that affected these sediments.Stable isotope variations of δ18O and δ13C in carbonate rocks show two different trends. High depletion of δ18O and low variation of δ13C probably reflect increasing temperatures during burial diagenesis, while the higher depletion in carbon isotope values with low variations in oxygen isotopes are related to fresh water flushing during meteoric diagenesis. Negative values of carbon isotopes may have also resulted from organic matter alteration during penetration of meteoric water. Fe and Mn enrichment with depletion of δ18O also supports the contention that alteration associated with higher depletion in carbon isotope values with low variations in oxygen isotopes took place during meteoric diagenesis. The presence of bright luminescence indicates redox conditions during precipitation of calcite cement

Eastern Mediterranean hydroclimate over the late glacial and Holocene, reconstructed from the sediments of Nar lake, central Turkey, using stable isotopes and carbonate mineralogy

There is a lack of high-resolution records of hydroclimate variability in the Eastern Mediterranean from the late glacial and early Holocene. More knowledge of the speed of climate shifts and the degree to which they were synchronous with changes in the North Atlantic or elsewhere is required to understand better the controls on Eastern Mediterranean climate. Using endogenic carbonate from a sediment sequence from Nar Gölü, a maar lake in central Turkey, dated by varve counting and uranium-thorium methods, we present high-resolution (∼25 years) oxygen (δ18O) and carbon isotope records, supported by carbonate mineralogy data, spanning the late glacial and Holocene. δ18Ocarbonate at Nar Gölü has been shown previously to be a strong proxy for regional water balance. After a dry period (i.e. evaporation far exceeding precipitation) in the Younger Dryas, the data show a transition into the relatively wetter early Holocene. In the early Holocene there are two drier periods that appear to peak at ∼9.3 ka and ∼8.2 ka, coincident with cooling ‘events’ seen in North Atlantic records. After this, and as seen in other records from the Eastern Mediterranean, there is a millennial-scale drying trend through the Mid Holocene Transition. The relatively dry late Holocene is punctuated by centennial-scale drought intervals, at the times of 4.2 ka ‘event’ and Late Bronze Age societal ‘collapse’. Overall, we show that central Turkey is drier when the North Atlantic is cooler, throughout this record and at multiple timescales, thought to be due to a weakening of the westerly storm track resulting from reduced cyclogenesis in the North Atlantic. However, some features, such as the Mid Holocene Transition and the fact the early Holocene dry episodes at Nar Gölü are of a longer duration than the more discrete ‘events’ seen in North Atlantic records, imply there are additional controls on Eastern Mediterranean hydroclimate