Knowledge and attitudes of men to prostate cancer

Objective: To ascertain the current level of understanding about prostate cancer (PCa), including treatment options and potential side effects of treatment, among older men. Design and Setting: Questionnaires administered by general practitioners (GPs) in 5 general practices in the Perth metropolitan and regional areas of Western Australia. Participants: Convenience sample of men aged 40-80 years (n=503) with or without prostate cancer presenting for routine consultations. Main outcome measures: Knowledge and attitudes of men to prostate cancer Results: Eighty percent of men did not know the function of the prostate and 48% failed to identify PCa as the most common internal cancer in men. Thirty-five percent had no knowledge of the treatments for PCa and 53% had no knowledge of the side effects of treatments. Asked how they would arrive at a decision about treatment, 70% stated they would ask the GP/specialist for all their options and then decide themselves. Conclusion: This study confirms a deficit in knowledge of the disease among men in the at risk age group. Lack of knowledge encompassed areas which could delay diagnosis and hence treatment. Overall the population preferred some GP/specialist involvement in treatment decision making

Influence of surgical resection no plasma Endoglin (CD105) level in non-small cell lung cancer patients

Background and Aim: Endoglin is a proliferation-associated antigen on endothelial cells and essential for angiogenesis. Soluble endoglin (s‑endoglin), formed by proteolytic cleavage of ectodomain of membrane receptor could be an indicator of tumor‑activated endothelium. The aim of present study was to analyze changes of s‑endoglin level in plasma of lung cancer patients following surgical resection and to estimate the correlation of s‑endoglin with other soluble receptors, sTie2 and sVEGF R1. Patients and Methods: The study group consisted of 37 patients with stage I of non-small cell lung cancer. Plasma concentrations of s‑endoglin, sTie2 and sVEGF R1 were evaluated by ELISA, three times: before surgical resection and on postoperative day 7 and 30. Results: The median of s‑endoglin concentration decreased significantly on postoperative day 7 when compared with preoperative level and next increased on 30th day and it was comparable with that before surgery. s-Endoglin correlated with another soluble receptors, with sTie2 both before surgery (r=0.44) and on postoperative day 7 (r=0.52) and on 30th day (r=0.58), with sVEGF R1 — only on postoperative day 7 (r=0.75). Conclusion: The increased level of serum endoglin in lung cancer patients compared to controls and its changes after surgical treatment suggest potential application of soluble form of endoglin as potential tumor marker

Generation of versatile ss-dsDNA hybrid substrates for single-molecule analysis.

Here, we describe a rapid and versatile protocol to generate gapped DNA substrates for single-molecule (SM) analysis using optical tweezers via site-specific Cas9 nicking and force-induced melting. We provide examples of single-stranded (ss) DNA gaps of different length and position. We outline protocols to visualize these substrates by replication protein A-enhanced Green Fluorescent Protein (RPA-eGFP) and SYTOX Orange staining using commercially available optical tweezers (C-TRAP). Finally, we demonstrate the utility of these substrates for SM analysis of bidirectional growth of RAD-51-ssDNA filaments. For complete details on the use and execution of this protocol, please refer to Belan et al. (2021)

Equivalent linearization technique in nonlinear stochastic dynamics of a cable-mass system with time-varying length

In this paper the transverse vibrations of a vertical cable carrying a concentrated mass at its lower end and moving slowly vertically within the host structure are considered. It is assumed that longitudinal inertia of the cable can be neglected, with the longitudinal motion of the concentrated mass coupled with the lateral motion of the cable. An expansion of the lateral displacement of a cable in terms of approximating functions is used. The excitation acting upon the cable-mass system is a base-motion excitation due to the sway motion of a host tall structure. Such a motion of a structure often results due to action of the wind, hence it may be adequately idealized as a narrow-band random process. The narrow-band process is represented as the output of a system of two linear filters to the input in a form of a Gaussian white noise process. The non-linear problem is dealt with by an equivalent linearization technique, where the original non-linear system is replaced with an equivalent linear one, whose coefficients are determined from the condition of minimization of a mean-square error between the non-linear and the linear systems. The mean value and variance of the transverse displacement of the cable as well as those of the longitudinal motion of the lumped mass are determined with the aid of an equivalent linear system and compared with the response of the original non-linear system subjected to the deterministic harmonic excitation

Development of cryopreservation for Loxocarya cinerea - an endemic Australian plant species important for post-mining restoration

We report the development of a cryopreservation protocol for the endemic Western Australian plant species Loxocarya cinerea (Restionaceae). Shoot tips from two genotypes, SXH404 and SXH804, were cryopreserved using the droplet-vitrification technique. Control explants, which were cryoprotected, but not cooled, showed regeneration for both genotypes (SXH404, 22.1 ± 5.9%; SXH804, 67.7 ± 9.6%). Extension of incubation in PVS2 from 30 to 60 min did not lead to survival after cryopreservation. Thermal analysis using differential scanning calorimetry confirmed the beneficial effect of a loading phase but also revealed no or very little ice formation after cryoprotection of shoot tips in other treatments. Regeneration following cryopreservation was obtained for genotype SXH804 (4.3 ± 2.1%) but not for SXH404. Regenerated explants of L. cinerea SXH804 were morphologically identical to tissue-cultured plants. As an alternative to shoot tips, callus tissues of clone SXH404 were successfully cryopreserved (>66.7% post LN survival) using the same protocol

Non-linear dynamic response of a cable system with a tuned mass damper to stochastic base excitation via equivalent linearization technique

Abstract: Non-linear dynamic model of a cable–mass system with a transverse tuned mass damper is considered. The system is moving in a vertical host structure therefore the cable length varies slowly over time. Under the time-dependent external loads the sway of host structure with low frequencies and high amplitudes can be observed. That yields the base excitation which in turn results in the excitation of a cable system. The original model is governed by a system of non-linear partial differential equations with corresponding boundary conditions defined in a slowly time-variant space domain. To discretise the continuous model the Galerkin method is used. The assumption of the analysis is that the lateral displacements of the cable are coupled with its longitudinal elastic stretching. This brings the quadratic couplings between the longitudinal and transverse modes and cubic nonlinear terms due to the couplings between the transverse modes. To mitigate the dynamic response of the cable in the resonance region the tuned mass damper is applied. The stochastic base excitation, assumed as a narrow-band process mean-square equivalent to the harmonic process, is idealized with the aid of two linear filters: one second-order and one first-order. To determine the stochastic response the equivalent linearization technique is used. Mean values and variances of particular random state variable have been calculated numerically under various operational conditions. The stochastic results have been compared with the deterministic response to a harmonic process base excitation

A multivariate complexity analysis of the material consumption scheduling problem

The NP-hard problem Material Consumption Scheduling and related problems have been thoroughly studied since the 1980’s. Roughly speaking, the problem deals with scheduling jobs that consume non-renewable resources—each job has individual resource demands. The goal is to minimize the makespan. We focus on the single-machine case without preemption: from time to time, the resources of the machine are (partially) replenished, thus allowing for meeting a necessary precondition for processing further jobs. We initiate a systematic exploration of the parameterized computational complexity landscape of Material Consumption Scheduling , providing parameterized tractability as well as intractability results. Doing so, we mainly investigate how parameters related to the resource supplies influence the problem’s computational complexity. This leads to a deepened understanding of this fundamental scheduling problem

A Multivariate Complexity Analysis of the Material Consumption Scheduling Problem

The NP-hard MATERIAL CONSUMPTION SCHEDULING Problem and closely related problems have been thoroughly studied since the 1980's. Roughly speaking, the problem deals with minimizing the makespan when scheduling jobs that consume non-renewable resources. We focus on the single-machine case without preemption: from time to time, the resources of the machine are (partially) replenished, thus allowing for meeting a necessary pre-condition for processing further jobs, each of which having individual resource demands. We initiate a systematic exploration of the parameterized (exact) complexity landscape of the problem, providing parameterized tractability as well as intractability results. Doing so, we mainly investigate how parameters related to the resource supplies influence the computational solvability. Thereby, we get a deepened understanding of the algorithmic complexity of this fundamental scheduling problem.Comment: Accepted for publication in The Thirty-Fifth AAAI Conference on Artificial Intelligence (AAAI-21