Plastination Procedure @ PCOM: Current Practice and Future Uses

Introduction: Since its invention by German anatomist Gunther von Hagens, the process of forced-impregnation plastination of organic specimens has become the standard for the preservation of biological tissue specimens. This practice serves as the most practical method to preserve these specimens for study and is utilized at PCOM regularly for this purpose. During the steps of plastination, aqueous and lipid tissues are replaced by a curable polymer to produce plastinates that do not decompose, can be handled without gloves, and retain most characteristics of the original specimens. For decades, PCOM used this method to prepare a permanent teaching collection in support of medical education. In the last five years, the lab has been reactivated and prepares specimens for allied health professional education, enrichment of the Doctor of Osteopathy curriculum, and outreach at regional institutions (e.g., The Franklin Institute, The Nebinger School, etc.). Objectives: The purpose of this poster is to inform the PCOM community of current plastination practices and suggest future uses. Methods: To prepare specimens for plastination, they must be preserved in fixative. We currently dissect and stage all tissues after the fixative process. Dissections are prepared by work-study students at PCOM who have completed the relevant anatomy course (interested students please contact Dr. Claeson). After fixation, tissues are dehydrated in progressively more concentrated washes of cold-temperature acetone (-20ºC) until concentration is between 98-100%. After dehydration, they are placed into a silicone polymer bath and brought to room temperature. The room temperature bath technique is the primary deviation from von Hagen (1977). Once at room temperature in the bath, vacuum pressure is used to replace the acetone that fills each cell with silicone. A hardening agent is then administered to finish the process. Results & Conclusions: Current initiatives have included building a collection of heart specimens to support a cardiac workshop which pairs anatomy and physiology. Most recently, a brain anatomy collection is being built. Brain specimens include axial cross sections, whole and half brains, and pathological specimens. These specimens are currently used at many outreach events and will be incorporated into a featured anatomy series as part of the medical school curriculum. Future research initiatives may also begin via current practices

Optimisation of the guided wave technology for inspection and health monitoring of polymer matrix composites

Polymer Matrix Composites (PMCs) are widely used in weight sensitive sectors (such as aircraft and wind turbine blades) where sudden failure of the structures can have a significant impact on the economy and more importantly, can cause life threatening situations. PMCs fail due to various defects, which can arise during the manufacturing stage and can continue growing during the component’s service life until its often unexpected failure. Delaminations are the most common defects in fibre-composites and several Non-destructive testing (NDT) techniques are available for their detection, however they generally require having access to all parts of the components to guarantee defect detection. The Guided Wave Testing (GWT) method can be used to inspect the component without requiring access to all that component however, it still needs further development before it can be accepted as a standard inspection and health monitoring technique for PMCs. The main goal of this thesis is to assess the defect detection capability of GWT for delamination and especially during three-point loading by validating experimental protocols designed for a specific GFRP sample. One of these protocols will also help to identify the potential precursors or warning signs of an imminent failure in composites

Changes in serogroup and genotype prevalence among carried meningococci in the United Kingdom during vaccine implementation.

BACKGROUND: Herd immunity is important in the effectiveness of conjugate polysaccharide vaccines against encapsulated bacteria. A large multicenter study investigated the effect of meningococcal serogroup C conjugate vaccine introduction on the meningococcal population. METHODS: Carried meningococci in individuals aged 15-19 years attending education establishments were investigated before and for 2 years after vaccine introduction. Isolates were characterized by multilocus sequence typing, serogroup, and capsular region genotype and changes in phenotypes and genotypes assessed. RESULTS: A total of 8462 meningococci were isolated from 47 765 participants (17.7%). Serogroup prevalence was similar over the 3 years, except for decreases of 80% for serogroup C and 40% for serogroup 29E. Clonal complexes were associated with particular serogroups and their relative proportions fluctuated, with 12 statistically significant changes (6 up, 6 down). The reduction of ST-11 complex serogroup C meningococci was probably due to vaccine introduction. Reasons for a decrease in serogroup 29E ST-254 meningococci (from 1.8% to 0.7%) and an increase in serogroup B ST-213 complex meningococci (from 6.7% to 10.6%) were less clear. CONCLUSIONS: Natural fluctuations in carried meningococcal genotypes and phenotypes a can be affected by the use of conjugate vaccines, and not all of these changes are anticipatable in advance of vaccine introduction

Belief in conspiracy theories and intentions to engage in everyday crime

Belief in conspiracy theories is associated with negative outcomes such as political disengagement, prejudice, and environmental inaction. The current studies - one cross-sectional (N = 253) and one experimental (N = 120) - tested the hypothesis that belief in conspiracy theories would increase intentions to engage in everyday crime. Study 1 demonstrated that belief in conspiracy theories predicted everyday crime behaviours when controlling for other known predictors of everyday crime (e.g., Honesty-Humility). Study 2 demonstrated that exposure to conspiracy theories (vs. control) increased intentions to engage in everyday crime in the future, through an increased feeling of anomie. The perception that others have conspired may therefore in some contexts lead to negative action rather than inaction

Discrimination, HIV conspiracy theories and pre-exposure prophylaxis acceptability in gay men

Background: Combination prevention, which includes PrEP, is essential for achieving the zero HIV infections target in the UK by 2030. It is important to assess attitudes towards PrEP in at risk-populations. This study focuses on the impact of discrimination and HIV conspiracy theorising on attitudes towards PrEP in gay men in the UK. Methods: 244 White British gay men completed a survey that included demographic questions, and measures of sexual health screening, hypervigilance, sexual orientation discrimination, quality of contact with healthcare professionals, belief in conspiracy theories, and attitudes towards PrEP. Data were analysed using multiple linear regression and mediation analysis. Results: Discrimination was positively correlated with HIV conspiracy beliefs and negatively correlated with PrEP acceptance. Mediation analyses demonstrated that the relationship between discrimination and attitudes towards PrEP was explained by HIV conspiracy theorising. Gay men who had attended a sexual health screen (vs. never attended) reported higher belief in HIV conspiracy theories. A further mediation analysis showed that reported poor contact with a healthcare professional was associated with an increased belief in HIV conspiracy theories, which was associated with negative attitudes towards PrEP. Both perceived discrimination and poor contact with a healthcare professional were exacerbated by hypervigilance. Conclusions: HIV conspiracy theorising is an important variable in understanding attitudes towards PrEP among gay men. Its roots are in adverse social experiences (e.g. discrimination, poor contact with healthcare professionals) and its consequences may be the rejection of PrEP. HIV prevention and PrEP campaigns must focus on prejudice reduction and challenging conspiracy beliefs