Identification of some o-aminophenones as secondary metabolites of Saccharomyces cerevisiae

Research NoteDuring fermentation of a peculiar model medium a strain of Saccharomyces cerevisiae var. cerevisiae (S1C) yeast from our collection was able to produce o-aminoacetophenone as well as other metabolites tentatively identified as o-aminopropiophenone and 3-(o-aminophenyl)-prop-1-en-3-one

The Linear Behaviour of Pathogen Strain of Bacillus anthracis A0843 in Anthrax Subcutaneous Challenge on Rabbit Model

Background: The pathogen strain of Bacillus anthracis A0843, isolated during an anthrax outbreak occurred in Italy, belongs to the Cluster A1a genotype 3. The authors show its activity underlining that the regular behaviour could make it useful as a reference strain for subcutaneous challenge in rabbit model for anthrax vaccines efficacy test. Italy doesn't use Ames strain because the restrictive measures, imposed after the bioterroristic events occurred in October 2001 in USA, reduced the movements of pathogen agents between reference laboratories in the world. It is necessary to adopt new rules that favour the security and the regularity of the research. Method: This study was done, during 3 years, on 50 New Zeeland rabbits, males and females, with a weigh between 1.200 and 1500 grams. The site of injection was back in the space between the two scapulae. It was used 20 LD50 (about 40.000 spores) of the pathogen strain according to the European Pharmacopoeia. Results: It was observed that anthrax begins to kills after 48 hours from the infection. At 72 hours the percentage of survival is 56,66%; at 96 hours is 30%. It was observed that two animals that survived after 120 hours from infection didn't die. Conclusion: The LD50 of B. anthracis strain A0843 in rabbit is 2.000 spores, less virulent then Ames strain which is characterized of a LD50 of about 1.200 spores. The standard amount of 20 DL50 (about 40.000 spores) of B. anthracis strain A0843 injected in subcutaneous area in rabbits shows a linear behaviour. The higher mortality is observed between 72 and 96 hours. All the animals died within 120 hours from the infection. None of the infected animals survived over this time and we consider it the survival line of anthrax subcutaneous challenge in rabbit. Technical support: Angela Aceti and Nicola Nigro Founds: Ricerca Corrente 2005 of Ministry of Health of Italy This research was done in according to the Decreto legislativo n.116/92 on animal welfar

A construção de uma rede de promoção do benefício da fbn através dos inoculantes: uma proposta metodológica de parceria público privado.

No Brasil, a agricultura é responsável por cerca de 30% do PIB, sendo desejável reduzir os custos de produção, aumentar a produtividade e minimizar os impactos ambientais. Esse trabalho visa apresentar um trabalho de articulação de parceiras público privado sob uma ótica neoinstitucionalista da economia com o propósito de fomentar a adoção da tecnologia de ?inoculantes? nas culturas de so ja, feijão-comum, feijão-caupi e milho. Através desta articulação vem sendo estabelecida uma rede com o apoio de 14 Unidades Descentralizada da Embrapa, Embrapa Sede (SECOM), a ANPII e demais parceiros (agricultores empresariais e familiares, gestores públicos, agentes da assistência técnica e extensão rural - ATER) envidando esforços tanto na divulgação sobre os benefícios desta tecnologia quanto na efetiva adoção da mesma pelo agricultor. Neste contexto, não se pode negar que há um papel preponderante da Embrapa no cenário nacional quanto à promoção de uma tecnologia inovadora em tempos de Programa ABC, pois a mesma permite manter a agricultura nacional competitiva (manutenção de índices de produtividade e custos de produção mais baixos) além de conferir um componente de sustentabilidade por parte do produtor rural. Bom para o agricultor, bom para o Brasil, bom para o planeta

Association of increased levels of homocysteine and peripheral arterial disease in a Japanese-Brazilian population

Objectives. Our aim was to evaluate the possible association between homocysteine levels and peripheral arterial disease (PAD) in a population-based study of Japanese-Brazilians.Materials and methods. This cross-sectional study was derived from a population-based survey on the prevalence of diabetes and associated diseases conducted in Japanese-Brazilians. A total of 1330 male and female subjects aged >= 30 years were submitted to clinical examination and laboratory procedures including homocysteine measurement. the ankle-brachial index (ABI) was calculated, subjects with ABI values <0.9 were diagnosed with PAD. the evaluable population included 1008 subjects. Logistic regression was used taking PAD as the dependent variable.Results. Mean age of the population was 56.5 years and overall prevalence of PAD was 20%. A worse cardiovascular profile was found in male patients, including significantly higher homocysteine levels (11.9 +/- 1.8 vs. 9.1 +/- 1.1 mu mol/L, p < 0.001). Men with PAD had higher prevalence rates of hyperhomocysteinemia compared to women (22.7% vs 7.6%). Univariate analysis showed an odds ratio of hyperhomocysteinemia for PAD of 1.51 [1.02-2.25] in men and 1. 69 [1.06-2.68] in women. After adjustment for other cardiovascular risk factors, higher levels of homocysteine were only significantly related to PAD in men.Conclusion. in a Japanese-Brazilian population, elevated levels of homocysteine are associated with PAD in men. Prospective studies are necessary to confirm this finding.Universidade Federal de São Paulo, Dept Surg, Postgrad Course Cardiac Vasc & Thorac Surg, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Surg, Div Vasc Surg, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Pediat, São Paulo, BrazilUniv São Paulo, Sch Publ Hlth, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Surg, Postgrad Course Cardiac Vasc & Thorac Surg, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Surg, Div Vasc Surg, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Pediat, São Paulo, BrazilWeb of Scienc

Genotyping and antibiotic resistance traits in Campylobacter jejuni and coli from pigs and wild boars in Italy

The present study investigated the genomic constitution and antimicrobial resistance (AMR) of 238 Campylobacter from pigs and wild boars in Italy between 2012 and 2019. Campylobacter strains were genotyped using multilocus sequence typing (MLST) and whole genome MLST (wgMLST), screened for antimicrobial resistance genes, and tested for phenotypic susceptibility to six different antibiotics. C. coli was detected in 98.31% and 91.66% of pigs and wild boars, while C. jejuni was isolated in the remaining cases. MLST assigned 73 STs and 13 STs in pigs and wild boars, respectively, including 44 novel STs. The predominant ST in pigs was ST-854 (12.36%), followed by ST-9264 (6.18%). ST-1055 and ST-1417 were predominant in wild boars (30% and 13.33%, respectively). The minimum spanning tree using 1,121 global MLST profiles showed specific Italian clusters and a clear separation between pig and wild boar profiles. The wgMLST confirmed the MLST clustering and revealed a high genetic diversity within C. coli population in Italy. Minimum inhibitory concentrations (MIC) of six antibiotics revealed higher resistance in pigs to ciprofloxacin, nalidixic acid, streptomycin and tetracycline, compared to wild boar. In contrast, most strains were susceptible to gentamicin. Worrying levels of multidrug resistance (MDR) were observed mostly in pig isolates. Molecular screening of AMR mechanisms revealed the predominance of gyrA T86I substitution among fluoroquinolone- and quinolone-resistant isolates, and the 23S rRNA A2075G mutation among macrolide-resistant isolates. Other resistance determinants were observed: (i) tet(O) gene was present among tetracycline-resistant isolates; (ii) rpsL and aph(3’)-III genes conferring resistance to aminoglycosides, were identified only in streptomycin or gentamicin-resistant pig isolates; (iii) cmeA, cmeB, cmeC, cmeR genes responsible of pump efflux mechanisms, were observed in almost all the strains; (iv) OXA-61, encoding β-lactamase, was found in the half of the strains. Genotypic and phenotypic AMR profiling was fairly correlated for quinolones/fluoroquinolones. Campylobacter infection is common also in wild boar populations in Italy, suggesting that wild boars could be a reservoir of resistant and multi-resistant Campylobacter species, which may be of public health concern. The present study adds to our knowledge on the epidemiological and ecological traits of this pathogen in domesticated and wild swine

Antimicrobial resistance genotypes and phenotypes of Campylobacter jejuni isolated in Italy from humans, birds from wild and urban habitats, and poultry

Campylobacter jejuni, a common foodborne zoonotic pathogen, causes gastroenteritis worldwide and is increasingly resistant to antibiotics. We aimed to investigate the antimicrobial resistance (AMR) genotypes of C. jejuni isolated from humans, poultry and birds from wild and urban Italian habitats to identify correlations between phenotypic and genotypic AMR in the isolates. Altogether, 644 C. jejuni isolates from humans (51), poultry (526) and wild- and urban-habitat birds (67) were analysed. The resistance phenotypes of the isolates were determined using the microdilution method with EUCAST breakpoints, and AMR-associated genes and single nucleotide polymorphisms were obtained from a publicly available database. Antimicrobial susceptibility testing showed that C. jejuni isolates from poultry and humans were highly resistant to ciprofloxacin (85.55% and 76.47%, respectively), nalidixic acid (75.48% and 74.51%, respectively) and tetracycline (67.87% and 49.02%, respectively). Fewer isolates from the wild- and urban-habitat birds were resistant to tetracycline (19.40%), fluoroquinolones (13.43%), and quinolone and streptomycin (10.45%). We retrieved seven AMR genes (tet (O), cmeA, cmeB, cmeC, cmeR, blaOXA-61 and blaOXA- 184) and gyrA-associated point mutations. Two major B-lactam genes called blaOXA-61 and blaOXA-184 were prevalent at 62.93% and 82.08% in the poultry and the other bird groups, respectively. Strong correlations between genotypic and phenotypic resistance were found for fluoroquinolones and tetracycline. Compared with the farmed chickens, the incidence of AMR in the C. jejuni isolates from the other bird groups was low, confirming that the food-production birds are much more exposed to antimicrobials. The improper and overuse of antibiotics in the human population and in animal husbandry has resulted in an increase in antibiotic-resistant infections, particularly fluoroquinolone resistant ones. Better understanding of the AMR mechanisms in C. jejuni is necessary to develop new strategies for improving AMR programs and provide the most appropriate therapies to human and veterinary populations

The CLEAR 2007 Evaluation

Abstract. This paper is a summary of the 2007 CLEAR Evaluation on the Classification of Events, Activities, and Relationships which took place in early 2007 and culminated with a two-day workshop held in May 2007. CLEAR is an international effort to evaluate systems for the perception of people, their activities, and interactions. In its second year, CLEAR has developed a following from the computer vision and speech communities, spawning a more multimodal perspective of research eval-uation. This paper describes the evaluation tasks, including metrics and databases used, and discusses the results achieved. The CLEAR 2007 tasks comprise person, face, and vehicle tracking, head pose estimation, as well as acoustic scene analysis. These include subtasks performed in the visual, acoustic and audio-visual domains for meeting room and surveillance data.

The non-Verbal Structure of Patient Case Discussions in Multidisciplinary Medical Team Meetings

Meeting analysis has a long theoretical tradition in social psychology, with established practical rami?cations in computer science, especially in computer supported cooperative work. More recently, a good deal of research has focused on the issues of indexing and browsing multimedia records of meetings. Most research in this area, however, is still based on data collected in laboratories, under somewhat arti?cial conditions. This paper presents an analysis of the discourse structure and spontaneous interactions at real-life multidisciplinary medical team meetings held as part of the work routine in a major hospital. It is hypothesised that the conversational structure of these meetings, as indicated by sequencing and duration of vocalisations, enables segmentation into individual patient case discussions. The task of segmenting audio-visual records of multidisciplinary medical team meetings is described as a topic segmentation task, and a method for automatic segmentation is proposed. An empirical evaluation based on hand labelled data is presented which determines the optimal length of vocalisation sequences for segmentation, and establishes the competitiveness of the method with approaches based on more complex knowledge sources. The effectiveness of Bayesian classi?cation as a segmentation method, and its applicability to meeting segmentation in other domains are discusse

Conservação de ecossistemas e redução da pobreza.

Este capítulo aborda a contribuição da Empresa Brasileira de Pesquisa Agropecuária (Embrapa) na execução de políticas públicas para atingir a meta 15.9 do Objetivo de Desenvolvimento Sustentável 15 (Nações Unidas, 2018): Até 2020, integrar os valores dos ecossistemas e da biodiversidade ao planejamento nacional e local, nos processos de desenvolvimento, nas estratégias de redução da pobreza e nos sistemas de contas. A capacidade de geração e manutenção dos serviços ambientais (regulação do clima e da água; polinização; provisão de água, fibras, madeira e alimentos; controle de erosão, dentre outros) nos diversos ambientes pode se encontrar comprometida devido aos usos inadequados ou superestimados e às alterações climáticas. A Comissão Econômica para a América Latina e o Caribe (Cepal), em dezembro de 2017, lançou um informe em que menciona que os níveis de pobreza extrema na região aumentaram em 2015 e 2016, mantendo-se estáveis em 2017. Os números giram em torno de 10% da população, com predomínio de crianças, adolescentes, jovens, mulheres e populações em áreas rurais. Isso demonstra a grande importância do estabelecimento de políticas públicas para esse contingente. No que tange ao meio rural, algumas iniciativas têm sido adotadas, entre elas o pagamento por serviços ambientais (PSA) ou ecossistêmicos (PSE) e as estratégias de manejo associadas à diminuição dos impactos negativos de uma agricultura baseada em alta demanda de insumos sintéticos. Para Wunder (2017), o PSA é uma transação voluntária, na qual um serviço ambiental bem definido ou um uso da terra que possa garantir tal serviço, é comprado por, pelo menos, um comprador e fornecido por, no mínimo, um provedor, desde que o provedor garanta a provisão desse serviço. O PSA surge como uma maneira de conferir valor monetário aos serviços ambientais gerados, tornando sua oferta uma parte da decisão estratégica dos agentes, uma vez que os usuários recebem um incentivo direto ao tornar suas práticas mais sustentáveis (Pagiola et al., 2002)