909 research outputs found
Cross-scale Urban Land Cover Mapping: Empowering Classification through Transfer Learning and Deep Learning Integration
Urban land cover mapping is essential for effective urban planning and resource management. Thanks to its ability to extract intricate features from urban datasets, deep learning has emerged as a powerful technique for urban classification. The U-net architecture has achieved state-of-the-art land cover classification performance, highlighting its potential for mapping urban trees at different spatial scales. However, deep learning approaches often require large, labeled datasets, which are challenging to acquire for specific urban contexts. Transfer learning addresses this limitation by leveraging pre-trained deep learning models on extensive datasets and adapting them to smaller urban datasets with limited labeled samples. Transfer learning can enhance classification performance and generalization ability. In this study, we proposed a novel cross-scale framework that integrates transfer learning and deep learning for urban land cover mapping. The framework utilizes pre-trained deep learning models, trained on diverse urban datasets, as a foundation for classification. These models are then finetuned using transfer learning techniques on smaller urban datasets, tailoring them to the specific characteristics of the target urban context. To evaluate the effectiveness and feasibility of the proposed framework, extensive evaluations are conducted across different cities and years. Performance metrics such as accuracy and dice score are employed to assess the framework\u27s classification capabilities. The results of this study contribute to advancing the field of urban classification by demonstrating the effectiveness and feasibility of the cross-scale framework. By combining transfer learning and deep learning, the framework improves classification accuracy, efficiency, and scalability in urban land cover mapping tasks. Leveraging the strengths of transfer learning and deep learning holds great promise for accurate and efficient urban land cover mapping, providing valuable insights for urban planning and resource management decision-making
Matrix decomposition algorithms for the C(0)-quadratic finite element Galerkin method
Explicit expressions for the eigensystems of one-dimensional finite element Galerkin (FEG) matrices based on C-0 piecewise quadratic polynomials are determined. These eigensystems are then used in the formulation of fast direct methods, matrix decomposition algorithms (MDAs), for the solution of the FEG equations arising from the discretization of Poisson's equation on the unit square subject to several standard boundary conditions. The MDAs employ fast Fourier transforms and require O(N-2 log N) operations on an N x N uniform partition. Numerical results are presented to demonstrate the efficacy of these algorithms
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Oxidized phospholipids are proinflammatory and proatherogenic in hypercholesterolaemic mice.
Oxidized phospholipids (OxPL) are ubiquitous, are formed in many inflammatory tissues, including atherosclerotic lesions, and frequently mediate proinflammatory changes 1 . Because OxPL are mostly the products of non-enzymatic lipid peroxidation, mechanisms to specifically neutralize them are unavailable and their roles in vivo are largely unknown. We previously cloned the IgM natural antibody E06, which binds to the phosphocholine headgroup of OxPL, and blocks the uptake of oxidized low-density lipoprotein (OxLDL) by macrophages and inhibits the proinflammatory properties of OxPL2-4. Here, to determine the role of OxPL in vivo in the context of atherogenesis, we generated transgenic mice in the Ldlr-/- background that expressed a single-chain variable fragment of E06 (E06-scFv) using the Apoe promoter. E06-scFv was secreted into the plasma from the liver and macrophages, and achieved sufficient plasma levels to inhibit in vivo macrophage uptake of OxLDL and to prevent OxPL-induced inflammatory signalling. Compared to Ldlr-/- mice, Ldlr -/- E06-scFv mice had 57-28% less atherosclerosis after 4, 7 and even 12 months of 1% high-cholesterol diet. Echocardiographic and histologic evaluation of the aortic valves demonstrated that E06-scFv ameliorated the development of aortic valve gradients and decreased aortic valve calcification. Both cholesterol accumulation and in vivo uptake of OxLDL were decreased in peritoneal macrophages, and both peritoneal and aortic macrophages had a decreased inflammatory phenotype. Serum amyloid A was decreased by 32%, indicating decreased systemic inflammation, and hepatic steatosis and inflammation were also decreased. Finally, the E06-scFv prolonged life as measured over 15 months. Because the E06-scFv lacks the functional effects of an intact antibody other than the ability to bind OxPL and inhibit OxLDL uptake in macrophages, these data support a major proatherogenic role of OxLDL and demonstrate that OxPL are proinflammatory and proatherogenic, which E06 counteracts in vivo. These studies suggest that therapies inactivating OxPL may be beneficial for reducing generalized inflammation, including the progression of atherosclerosis, aortic stenosis and hepatic steatosis
Reed Parrotbill nest predation by tidal mudflat crabs: Evidence for an ecological trap?
Understanding the relationships between nesting habitat quality and predation risk is
essential for developing appropriate conservation management for threatened species. This is particularly
relevant where anthropogenic pressures could decouple the environmental cues used by birds to assess
nesting habitat quality from increased predation risk. In this study, we conducted a series of surveys and
nest experiments to examine the nest predation rates of Reed Parrotbill (Paradoxornis heudei ) a passerine
bird between inland and tidal reed-bed wetland habitats, at Yellow River Delta National Nature Reserve,
Eastern China during 2008, and 2010â2012. We found significant differences in the habitat structural
characteristics between the two adjacent wetland habitats that are critical for Reed Parrotbill nest-site
selection. Experimental trials using recently constructed and abandoned Reed Parrotbill nests as âartificial
nests, quail eggs and predator-exclusion measures, revealed that tidal mudflat crab (Helice tientsinensis)
was the primary cause of Reed Parrotbill egg predation in tidal reed-bed habitat. Annual predation rates of
real nests from inland reed-bed habitat varied from 35% to 68%, and predation rates of artificial nests were
much lower than those from real nests. Pitfall sampling revealed that the abundance of tidal mudflat crabs
was significantly higher in tidal reed-bed habitat. Our data suggested that Reed Parrotbills breeding in
tidal reed-bed habitats may be highly vulnerable due to extremely high rates of nest predation (up to
100%), caused primarily by the high density of tidal mudflat crabs. The incongruence between nest-site
habitat preference and nest survival indicated an ecological trap scenario, which requires further studies on
its proximate and ultimate causes as well as the development of effective conservation management for the
Reed Parrotbill
Concomitant Retrograde Coronary Venous Infusion of Basic Fibroblast Growth Factor Enhances Engraftment and Differentiation of Bone Marrow Mesenchymal Stem Cells for Cardiac Repair after Myocardial Infarction.
AIM: Basic fibroblast growth factor (bFGF) increases the migration and viability of bone marrow mesenchymal stem cells (MSCs) in vitro. Retrograde coronary venous infusion can provide both increased regional bFGF concentrations and homogeneous cell dissemination. We determined whether retrograde delivery of bFGF enhances the potency of transplanted MSCs for cardiac repair in a canine infarct model.
METHODS AND RESULTS: Under hypoxic conditions, cellular migration was significantly increased in MSCs co-cultured with bFGF compared to vascular endothelial growth factor or insulin-like growth factor, and bFGF promoted MSCs differentiation into a cardiomyocyte phenotype. A canine infarct model was employed by coronary ligation. One week later, animals were subjected to retrograde infusion of combination bFGF (200ng/mL) and MSCs (1Ă10(8) cells) (n=5), MSCs (1Ă10(8) cells, n=5), bFGF (200ng/mL, n=5), or placebo (phosphate-buffered saline, n=3). Four weeks after infusion, only the bFGF+MSCs therapy exhibited significantly increased left ventricular ejection fraction (LVEF) by echocardiography (p
CONCLUSIONS: Retrograde coronary venous bFGF infusion augments engraftment and differentiation capacity of transplanted MSCs, recovering cardiac function and preventing adverse remodeling. This novel combined treatment and delivery method is a promising strategy for cardiac repair after ischemic injury
Sox2 Cooperates with Inflammation-Mediated Stat3 Activation in the Malignant Transformation of Foregut Basal Progenitor Cells
SummarySox2 regulates the self-renewal of multiple types of stem cells. Recent studies suggest it also plays oncogenic roles in the formation of squamous carcinoma in several organs, including the esophagus where Sox2 is predominantly expressed in the basal progenitor cells of the stratified epithelium. Here, we use mouse genetic models to reveal a mechanism by which Sox2 cooperates with microenvironmental signals to malignantly transform epithelial progenitor cells. Conditional overexpression of Sox2 in basal cells expands the progenitor population in both the esophagus and forestomach. Significantly, carcinoma only develops in the forestomach, where pathological progression correlates with inflammation and nuclear localization of Stat3 in progenitor cells. Importantly, co-overexpression of Sox2 and activated Stat3 (Stat3C) also transforms esophageal basal cells but not the differentiated suprabasal cells. These findings indicate that basal stem/progenitor cells are the cells of origin of squamous carcinoma and that cooperation between Sox2 and microenvironment-activated Stat3 is required for Sox2-driven tumorigenesis
Antibiotic mediated synthesis of gold nanoparticles with potent antimicrobial activity and their application in antimicrobial coatings
We report a one-pot synthesis of spherical gold nanoparticles (52-22 nm) and their capping with cefaclor, a second-generation antibiotic, without use of other chemicals. The differently sized gold nanoparticles were fabricated by controlling the rate of reduction of gold ions in aqueous solution by varying the reaction temperature (20-70 C). The primary amine group of cefaclor acted as both the reducing and capping agent for the synthesis of gold nanoparticles leaving the b-lactam ring of cefaclor available for activity against microbes. Antimicrobial testing showed that cefaclor reduced gold nanoparticles have potent antimicrobial activity against both Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria as compared to cefaclor or gold nanoparticles alone. The minimum inhibition concentrations (MICs) of cefaclor reduced gold nanoparticles were 10m gmL1 and 100m gmL1 for S. aureus and E. coli respectively. The cefaclor reduced gold nanoparticles were further coated onto poly(ethyleneimine) (PEI) modified glass surfaces to obtain antimicrobial coatings suitable for biomedical applications and were tested against E. coli as an exemplar of activity. The antimicrobial coatings were very robust under adverse conditions (pH 3 and 10), inhibited the growth of E. coli on their surfaces, and could be used many times with retained activity. Results from a combined spectroscopic (FTIR) and microscopic study (AFM) suggest that the action of these novel particles is through the combined action of cefaclor inhibiting the synthesis of the peptidoglycan layer and gold nanoparticles generating "holes" in bacterial cell walls thereby increasing the permeability of the cell wall, resulting in the leakage of cell contents and eventually cell death
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Loss of CD103+ DCs and Mucosal IL-17+ and IL-22+ Lymphocytes is Associated with Mucosal Damage in SIV Infection
HIV/SIV disease progression is associated with multifocal damage to the GI tract epithelial barrier that correlates with microbial translocation and persistent pathological immune activation but the underlying mechanisms remain unclear. Investigating alterations in mucosal immunity during SIV infection, we found that damage to the colonic epithelial barrier was associated with loss of multiple lineages of IL-17-producing lymphocytes, cells that microarray analysis showed express genes important for enterocyte homeostasis, including IL-22. IL-22-producing lymphocytes were also lost after SIV infection. Potentially explaining coordinate loss of these distinct populations, we also observed loss of CD103+ DCs after SIV infection which associated with loss of IL-17 and IL-22-producing lymphocytes. CD103+ DCs expressed genes associated with promotion of IL-17/IL-22+ cells, and co-culture of CD103+ DCs and naĂŻve T-cells led to increased IL17A and RORc expression in differentiating T-cells. These results reveal complex interactions between mucosal immune cell subsets providing potential mechanistic insights into mechanisms of mucosal immune dysregulation during HIV/SIV infection, and offer hints for development of novel therapeutic strategies to address this aspect of AIDS virus pathogenesis
Partial Wave Analysis of
BES data on are presented. The
contribution peaks strongly near threshold. It is fitted with a
broad resonance with mass MeV, width MeV. A broad resonance peaking at 2020 MeV is also required
with width MeV. There is further evidence for a component
peaking at 2.55 GeV. The non- contribution is close to phase
space; it peaks at 2.6 GeV and is very different from .Comment: 15 pages, 6 figures, 1 table, Submitted to PL
Measurement of the Inclusive Charm Cross Section at 4.03 GeV and 4.14 GeV
The cross section for charmed meson production at and 4.14
GeV has been measured with the Beijing Spectrometer. The measurement was made
using 22.3 of data collected at 4.03 GeV and 1.5
of data collected at 4.14 GeV. Inclusive observed cross sections for
the production of charged and neutral D mesons and momentum spectra are
presented. Observed cross sections were radiatively corrected to obtain tree
level cross sections. Measurements of the total hadronic cross section are
obtained from the charmed meson cross section and an extrapolation of results
from below the charm threshold.Comment: 11 pages, 13 figures. The top level tex file is paper.tex. It builds
the paper from other tex files in this .tar and the .eps file
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