Enhanced Second Harmonic Generation in Femtosecond Laser Inscribed Double-Cladding Waveguide of Nd:GdCOB Crystal

We report on the fabrication of double-cladding waveguides in Nd:GdCOB crystals by using femtosecond laser inscription with scanning speed of 0.5 mm/s. This prototype consists of two concentric tubular structures with nearly circular cross sections with different diameters. The fabricated cladding waveguides possess relatively low propagation losses of less than 0.65 dB/cm. The micro photoluminescence and second harmonic confocal images of the fabricated waveguides, which were performed by using a confocal microscope, have revealed that neither the fluorescence nor the nonlinear properties of the constituent crystals have been deteriorated during femtosecond lasermicromachining procedure. Under the pulsed laser pumping at fundamental wavelength of 1064 nm, the guided wave second harmonic generation (SHG) at 532 nm has been realized from the single and double cladding waveguides. Compared to the single-claddings (2.3 or 4.7%, depending on the diverse cladding diameters), the double cladding architecture has been found to be with enhanced SHG conversion efficiency (∼5.1%).The work was supported by the National Natural Science Foundation of China under Grants 11274203 and 11111130200, the Spanish Ministerio de Ciencia e Innovación (MICINN) through Consolider Program SAUUL CSD2007-00013 and Project FIS2009-09522, and the Centro de Láseres Pulsados (CLPU)

Morphological diversity of single neurons in molecularly defined cell types.

Dendritic and axonal morphology reflects the input and output of neurons and is a defining feature of neuronal types1,2, yet our knowledge of its diversity remains limited. Here, to systematically examine complete single-neuron morphologies on a brain-wide scale, we established a pipeline encompassing sparse labelling, whole-brain imaging, reconstruction, registration and analysis. We fully reconstructed 1,741 neurons from cortex, claustrum, thalamus, striatum and other brain regions in mice. We identified 11 major projection neuron types with distinct morphological features and corresponding transcriptomic identities. Extensive projectional diversity was found within each of these major types, on the basis of which some types were clustered into more refined subtypes. This diversity follows a set of generalizable principles that govern long-range axonal projections at different levels, including molecular correspondence, divergent or convergent projection, axon termination pattern, regional specificity, topography, and individual cell variability. Although clear concordance with transcriptomic profiles is evident at the level of major projection type, fine-grained morphological diversity often does not readily correlate with transcriptomic subtypes derived from unsupervised clustering, highlighting the need for single-cell cross-modality studies. Overall, our study demonstrates the crucial need for quantitative description of complete single-cell anatomy in cell-type classification, as single-cell morphological diversity reveals a plethora of ways in which different cell types and their individual members may contribute to the configuration and function of their respective circuits

A multimodal cell census and atlas of the mammalian primary motor cortex

ABSTRACT We report the generation of a multimodal cell census and atlas of the mammalian primary motor cortex (MOp or M1) as the initial product of the BRAIN Initiative Cell Census Network (BICCN). This was achieved by coordinated large-scale analyses of single-cell transcriptomes, chromatin accessibility, DNA methylomes, spatially resolved single-cell transcriptomes, morphological and electrophysiological properties, and cellular resolution input-output mapping, integrated through cross-modal computational analysis. Together, our results advance the collective knowledge and understanding of brain cell type organization: First, our study reveals a unified molecular genetic landscape of cortical cell types that congruently integrates their transcriptome, open chromatin and DNA methylation maps. Second, cross-species analysis achieves a unified taxonomy of transcriptomic types and their hierarchical organization that are conserved from mouse to marmoset and human. Third, cross-modal analysis provides compelling evidence for the epigenomic, transcriptomic, and gene regulatory basis of neuronal phenotypes such as their physiological and anatomical properties, demonstrating the biological validity and genomic underpinning of neuron types and subtypes. Fourth, in situ single-cell transcriptomics provides a spatially-resolved cell type atlas of the motor cortex. Fifth, integrated transcriptomic, epigenomic and anatomical analyses reveal the correspondence between neural circuits and transcriptomic cell types. We further present an extensive genetic toolset for targeting and fate mapping glutamatergic projection neuron types toward linking their developmental trajectory to their circuit function. Together, our results establish a unified and mechanistic framework of neuronal cell type organization that integrates multi-layered molecular genetic and spatial information with multi-faceted phenotypic properties

Second harmonic generation of violet light in femtosecond-laser-inscribed BiB3O6 cladding waveguides

Depressed cladding waveguide structures have been fabricated in BiB3O6 nonlinear crystal by using femtosecond (fs) laser inscription. The nonlinear properties of original BiB3O6 crystal have been well preserved within the waveguide volume. Under 800 nm continuous wave laser pumping, the guided-wave second harmonic generation of violet light at 400 nm has been realized, with a maximum power of ~1.05 mW and a conversion efficiency of ~0.98%/W

Femtosecond-Laser-Inscribed BiB3_{3}O6_{6} Nonlinear Cladding Waveguide for Second-Harmonic Generation

We report on the fabrication of a nonlinear cladding waveguide in BiB3O6 crystal by using femtosecond laser inscription. The waveguide (with a nearly circular cross section of 150 µm diameter) shows good guiding properties in two transverse polarizations. The guided-wave second-harmonic generation (SHG) at 532 nm green light has been realized under CW and pulsed wave pump at 1064 nm, based on the Type I birefringent phase matching configuration. The conversion efficiencies for CW and pulsed green laser SHG are 0.083 and 25%, respectively.This work was supported by the National Natural Science Foundation of China (No. 11111130200), the Spanish Ministerio de Ciencia e Innovación (MICINN) through Consolider Program SAUUL CSD2007-00013, and Project FIS2009-09522. Support from the Centro de LáseresPulsados (CLPU) is also acknowledged

Efficient Optical Waveguiding Enabled by Focused Proton Beam Writing in Nd:YCOB Crystal

We report on microfabrication and optical characterization of buried channel waveguides defined in Nd:YCOB crystal by focused proton beam writing (PBW). In the fabrication process, the focused proton beam irradiation creates a local material modification region with geometrically symmetric positive index changes at the end of the proton trajectory, where efficient optical waveguiding can be locally supported within a fiber-like channel structure. The impact of the proton fluence (with different values ranging from 1015 to 1016 cm−2) on the optical waveguiding performance is well studied. The experimental results of the optical waveguide properties are in fairly good agreement with the simulation results

A new osteichthyan from the late Silurian of Yunnan, China.

Our understanding of early gnathostome evolution has been hampered by a generally scant fossil record beyond the Devonian. Recent discoveries from the late Silurian Xiaoxiang Fauna of Yunnan, China, have yielded significant new information, including the earliest articulated osteichthyan fossils from the Ludlow-aged Kuanti Formation. Here we describe the partial postcranium of a new primitive bony fish from the Kuanti Formation that represents the second known taxon of pre-Devonian osteichthyan revealing articulated remains. The new form, Sparalepis tingi gen. et sp. nov., displays similarities with Guiyu and Psarolepis, including a spine-bearing pectoral girdle and a placoderm-like dermal pelvic girdle, a structure only recently identified in early osteichthyans. The squamation with particularly thick rhombic scales shares an overall morphological similarity to that of Psarolepis. However, the anterior flank scales of Sparalepis possess an unusual interlocking system of ventral bulges embraced by dorsal concavities on the outer surfaces. A phylogenetic analysis resolves Sparalepis within a previously recovered cluster of stem-sarcopterygians including Guiyu, Psarolepis and Achoania. The high diversity of osteichthyans from the Ludlow of Yunnan strongly contrasts with other Silurian vertebrate assemblages, suggesting that the South China block may have been an early center of diversification for early gnathostomes, well before the advent of the Devonian "Age of Fishes"

Second Harmonic Generation of Violet Light in Femtosecond-Laser-Inscribed BiB

We report on the second harmonic generation of violet light of a nonlinear cladding waveguide in BiB3O6 crystal produced by femtosecond laser inscription. Under continuous-wave pump laser at 800 nm, the guided second harmonic wave at 400 nm with a conversion efficiency of ~0.32% has been realized through the Type I birefringence phase matching configuration

Second Harmonic Generation of Violet Light in Femtosecond-Laser-Inscribed BiB3O6 Cladding Waveguides

We report on the second harmonic generation of violet light of a nonlinear cladding waveguide in BiB3O6 crystal produced by femtosecond laser inscription. Under continuous-wave pump laser at 800 nm, the guided second harmonic wave at 400 nm with a conversion efficiency of ~0.32% has been realized through the Type I birefringence phase matching configuration