Potential modulating effects of Allium mongolicum regel ethanol extract on rumen fermentation and biohydrogenation bacteria of dairy cows in vitro

The objective of this study was to evaluate the potential modulating effects of Allium mongolicum regel ethanol extract (AME) on rumen fermentation and biohydrogenation (BH) bacteria in vitro. Four Holstein cows were used as donors for the rumen fluid used in this study. In experiment 1, five treatments (supplemented with 0 mg/g, 1 mg/g, 2 mg/g, 3 mg/g, and 4 mg/g of AME based on fermentation substrate, respectively) were conducted to evaluate the effects of different levels of AME on fermentation status in vitro. The results showed that after 24 h of fermentation, MCP was reduced with AME supplementation (p < 0.05), and the multiple combinations of different combinations index (MFAEI) value was the highest with 3 mg/g of AME. In experiment 2, six treatments were constructed which contained: control group (A1); the unsaturated fatty acid (UFA) mixture at 3% concentration (A2); the mixture of A2 and 3 mg/g of AME (A3); 3 mg/g of AME (A4); the UFA mixture at 1.5% concentration (A5); the mixture of A5 and 3 mg/g of AME (A6). The abundance of bacterial species involved in BH was measured to evaluate the potential modulating effect of AME on rumen BH in vitro. Compared with the A1 group, the A3, A4, and A6 groups both showed significant decreases in the abundance of rumen BH microbial flora including Butyrivibrio proteoclasticus, Butyrivibrio fibrisolvens, Ruminococcus albus and Clostridium aminophilum (p < 0.01). The A3 group was less inhibitory than A4 in the abundance of B. proteoclasticus, B. fibrisolvens, and R. albus, and the inhibitory effect of the A6 group was higher than that of A4. In conclusion, the supplementation with 3 mg/g of AME could modulate the rumen fermentation and affect BH key bacteria, which suggests that AME may have the potential to inhibit the rumen BH of dairy cows

Effects of the Dietary Inclusion of <i>Allium mongolicum</i> Regel Extract on Serum Index and Meat Quality in Small-Tailed Han Sheep

The aim of this study was to evaluate the effects of Allium mongolicum Regel ethanol extract (AME) on the serum index and meat quality of lambs. A total of 30 male Small-tailed Han sheep (3 months old) with an average weight of 33.60 ± 1.23 kg were divided randomly into one of two groups: the control group (CON) was offered a basal diet, and the AME group was offered a basal diet with supplementation 2.8 g·lamb−1·day−1 AME. The trial lasted for 75 days. AME supplementation significantly decreased the concentration of triglyceride and total cholesterol (p p p p p > 0.05). However, AME changed the composition of free amino acids and promoted MUFA and PUFA deposition in the LD muscle of the lambs. These findings indicate that a diet supplemented with AME may improve the lipid metabolic capacity and meat quality of lambs