72 research outputs found
The zebrafish xenograft platform-A novel tool for modeling KSHV-associated diseases
Kaposi\u27s sarcoma associated-herpesvirus (KSHV, also known as human herpesvirus-8) is a gammaherpesvirus that establishes life-long infection in human B lymphocytes. KSHV infection is typically asymptomatic, but immunosuppression can predispose KSHV-infected individuals to primary effusion lymphoma (PEL); a malignancy driven by aberrant proliferation of latently infected B lymphocytes, and supported by pro-inflammatory cytokines and angiogenic factors produced by cells that succumb to lytic viral replication. Here, we report the development of the firs
The Vehicle, Spring 1985
Vol. 26, No. 2
Table of Contents
Beyond the FieldsKeila Tooleypage 3
Lonely Sculptor Accustomed to Living AloneMichelle Mitchellpage 4
Mona LisaBob Zordanipage 4
Poet Born in Pearl HarborAngelique Jenningspage 5
IntroductionsGraham Lewispage 6
Living InsideJennifer Soulepage 9
PictureKathy Greypage 10
Salvadore Dali in a Wheelchair on TVAngelique Jenningspage 11
Sonata in E FlatBecky Lawsonpage 12
Myopia and Wild KingdomMichelle Mitchellpage 12
On Becoming a GrandmotherKeila Tooleypage 13
A VisionJennifer D. Pringlepage 14
The Covered BridgeDebbie Woodleypage 14
Jacob\u27s LifeJoan Sebastianpage 15
ForgotGraham Lewispage 15
A Dozen and One TrainsongsAngelique Jenningspage 16
Women\u27s PlaceJennifer Soulepage 19
Night SailingKim Dumentatpage 20
She Isn\u27t There WhenMichelle Mitchellpage 20
A Case for the Common ColdMaggie Kennedypage 21
the cityTammy Batespage 22
The RattlesnakeEric S. McGeepage 22
New PictureKeila Tooleypage 23
Lewis and SinGraham Lewispage 24
Funny BarbecueBob Zordanipage 26
In a DreamF. Link Rapierpage 26
The Winter\u27s ColdJennifer Soulepage 27
Diary EntryTammy Batespage 27
Minor God and Patron Saint of Rabbits SpeaksAngelique Jenningspage 28
A MomentBrett Wilhelmpage 29
The Bishop SeatF. Link Rapierpage 30
The Thought of Being Rid of MyselfKeila Tooleypage 33
I Saw A ChildBea Cessnapage 33
Complacent gourmetGary Burrowspage 34
Night DreamsJennifer Soulepage 35
Changing ImagesAmy Callpage 35
Olsen Rug Co. Waterfall & ParkMaggie Kennedypage 36
Edge of the WildF. Link Rapierpage 37
DragonS. Hillpage 37
Harvests of CornBob Zordanipage 38
The Club JeromeGary Burrowspage 39
Tarzan And The CabPatrick Peterspage 39
The Rain That Never CameLynanne Feilenpage 40
Wonderment of the Far CrescentF. Link Rapierpage 40https://thekeep.eiu.edu/vehicle/1047/thumbnail.jp
CpG-free plasmids confer reduced inflammation and sustained pulmonary gene expression.
Pulmonary delivery of plasmid DNA (pDNA)/cationic liposome complexes is associated with an acute unmethylated CG dinucleotide (CpG)-mediated inflammatory response and brief duration of transgene expression. We demonstrate that retention of even a single CpG in pDNA is sufficient to elicit an inflammatory response, whereas CpG-free pDNA vectors do not. Using a CpG-free pDNA expression vector, we achieved sustained (≥56 d) in vivo transgene expression in the absence of lung inflammation
The Decreasing Significance of Stigma in the Lives of Bisexual Men: Keynote Address, Bisexual Research Convention, London
This article is constructed around a keynote address given at the Bisexual Research Convention, held in London 2010. The keynote was delivered by sociologist Eric Anderson, on behalf of himself and the other authors of this article. The keynote reflected upon a body of ongoing research, funded by the American Institute of Bisexuality and collected by this team of researchers, into the changing relationship between men and homophobia. It first contextualizes 20th-century attitudes toward homo/bisexuality before showing a declining significance of biphobia and homophobia in men's lives today. In accordance with the keynote, this article draws from preliminary findings of multiple ongoing studies of bisexual men in the United States and the United Kingdom
Riparian Plant Litter Quality Increases With Latitude
Plant litter represents a major basal resource in streams, where its decomposition is partly regulated by litter traits. Litter-trait variation may determine the latitudinal gradient in decomposition in streams, which is mainly microbial in the tropics and detritivore-mediated at high latitudes. However, this hypothesis remains untested, as we lack information on large-scale trait variation for riparian litter. Variation cannot easily be inferred from existing leaf-trait databases, since nutrient resorption can cause traits of litter and green leaves to diverge. Here we present the first global-scale assessment of riparian litter quality by determining latitudinal variation (spanning 107°) in litter traits (nutrient concentrations; physical and chemical defences) of 151 species from 24 regions and their relationships with environmental factors and phylogeny. We hypothesized that litter quality would increase with latitude (despite variation within regions) and traits would be correlated to produce ‘syndromes’ resulting from phylogeny and environmental variation. We found lower litter quality and higher nitrogen:phosphorus ratios in the tropics. Traits were linked but showed no phylogenetic signal, suggesting that syndromes were environmentally determined. Poorer litter quality and greater phosphorus limitation towards the equator may restrict detritivore-mediated decomposition, contributing to the predominance of microbial decomposers in tropical streams
Riparian plant litter quality increases with latitude
Plant litter represents a major basal resource in streams, where its decomposition is partly regulated by litter traits. Litter-trait variation may determine the latitudinal gradient in decomposition in streams, which is mainly microbial in the tropics and detritivore-mediated at high latitudes. However, this hypothesis remains untested, as we lack information on large-scale trait variation for riparian litter. Variation cannot easily be inferred from existing leaf-trait databases, since nutrient resorption can cause traits of litter and green leaves to diverge. Here we present the first global-scale assessment of riparian litter quality by determining latitudinal variation (spanning 107 degrees) in litter traits (nutrient concentrations; physical and chemical defences) of 151 species from 24 regions and their relationships with environmental factors and phylogeny. We hypothesized that litter quality would increase with latitude (despite variation within regions) and traits would be correlated to produce 'syndromes' resulting from phylogeny and environmental variation. We found lower litter quality and higher nitrogen: phosphorus ratios in the tropics. Traits were linked but showed no phylogenetic signal, suggesting that syndromes were environmentally determined. Poorer litter quality and greater phosphorus limitation towards the equator may restrict detritivore-mediated decomposition, contributing to the predominance of microbial decomposers in tropical streams.We thank the many assistants who helped with field work (Ana Chara-Serna, Francisco Correa-Araneda, Juliana Franca, Lina Giraldo, Stephanie Harper, Samuel Kariuki, Sylvain Lamothe, Lily Ng, Marcus Schindler, etc.), Cristina Grela Docal for helping with leaf chemical analyses, and Fernando Hiraldo (former director of EBD-CSIC) for his support. The study was funded by start-up funds from the Donana Biological Station (EBD-CSIC, Spain) and from Ikerbasque to LB, the Fundacao para a Ciencia e Tecnologia (FCT) strategic project ID/MAR/04292/2013 granted to MARE (Portugal), the 'BIOFUNCTION' project (CGL2014-52779-P) from the Spanish Ministry of Economy and Competitiveness (MINECO) and FEDER to LB and J. Pozo, and Basque Government funds (IT302-10) to J. Pozo
Identification and Classification of Conserved RNA Secondary Structures in the Human Genome
The discoveries of microRNAs and riboswitches, among others, have shown functional RNAs to be biologically more important and genomically more prevalent than previously anticipated. We have developed a general comparative genomics method based on phylogenetic stochastic context-free grammars for identifying functional RNAs encoded in the human genome and used it to survey an eight-way genome-wide alignment of the human, chimpanzee, mouse, rat, dog, chicken, zebra-fish, and puffer-fish genomes for deeply conserved functional RNAs. At a loose threshold for acceptance, this search resulted in a set of 48,479 candidate RNA structures. This screen finds a large number of known functional RNAs, including 195 miRNAs, 62 histone 3′UTR stem loops, and various types of known genetic recoding elements. Among the highest-scoring new predictions are 169 new miRNA candidates, as well as new candidate selenocysteine insertion sites, RNA editing hairpins, RNAs involved in transcript auto regulation, and many folds that form singletons or small functional RNA families of completely unknown function. While the rate of false positives in the overall set is difficult to estimate and is likely to be substantial, the results nevertheless provide evidence for many new human functional RNAs and present specific predictions to facilitate their further characterization
- …