1,993 research outputs found
Metastatic Carcinoma of Unknown Primary Presenting as Jugular Venous Thrombosis
Jugular venous thrombosis is unusual and is associated with central venous catheterisation, intravenous drug abuse and head and neck sepsis. It is rarely associated with malignancy. We report a case of metastatic carcinoma of unknown primary in a forty year old female which presented with jugular venous thrombosis. The discussion includes investigation and treatment options for this condition
The Leucine-Rich Repeat Receptor-Like Kinase BRASSINOSTEROID INSENSITIVE1-ASSOCIATED KINASE1 and the Cytochrome P450 PHYTOALEXIN DEFICIENT3 Contribute to Innate Immunity to Aphids in Arabidopsis
The importance of pathogen-associated molecular pattern-triggered immunity (PTI) against microbial pathogens has been recently demonstrated. However, it is currently unclear if this layer of immunity mediated by surface-localized pattern recognition receptors (PRRs) also plays a role in basal resistance to insects, such as aphids. Here, we show that PTI is an important component of plant innate immunity to insects. Extract of the green peach aphid (GPA; Myzus persicae) triggers responses characteristic of PTI in Arabidopsis (Arabidopsis thaliana). Two separate eliciting GPA-derived fractions trigger induced resistance to GPA that is dependent on the leucine-rich repeat receptor-like kinase BRASSINOSTEROID INSENSITIVE1-ASSOCIATED KINASE1 (BAK1)/SOMATIC-EMBRYOGENESIS RECEPTOR-LIKE KINASE3, which is a key regulator of several leucine-rich repeat-containing PRRs. BAK1 is required for GPA elicitor-mediated induction of reactive oxygen species and callose deposition. Arabidopsis bak1 mutant plants are also compromised in immunity to the pea aphid (Acyrthosiphon pisum), for which Arabidopsis is normally a nonhost. Aphid-derived elicitors induce expression of PHYTOALEXIN DEFICIENT3 (PAD3), a key cytochrome P450 involved in the biosynthesis of camalexin, which is a major Arabidopsis phytoalexin that is toxic to GPA. PAD3 is also required for induced resistance to GPA, independently of BAK1 and reactive oxygen species production. Our results reveal that plant innate immunity to insects may involve early perception of elicitors by cell surface-localized PRRs, leading to subsequent downstream immune signaling
Habitat Standardization of CPUE Indices: Research Needs
Habitat standardization for billfish CPUE offers a potentially useful alternative to the statistical procedures used in the past. However, most of the assumptions of the current habitatstandardization methodology remain untested and some are not consistent with current knowledge about the behavior of billfish. This paper outlines research required to ensure the methods for habitat standardization produce robust estimates of CPUE
Infrared chemical imaging through nondegenerate two-photon absorption in silicon-based cameras
Chemical imaging based on mid-infrared (MIR) spectroscopic contrast is an
important technique with a myriad of applications, including biomedical imaging
and environmental monitoring. Current MIR cameras, however, lack in performance
and are much less affordable compared to mature Si-based devices, which operate
in the visible and near-infrared. Here we demonstrate fast MIR chemical imaging
through non-degenerate two-photon absorption (NTA) in a standard Si-based
charge-coupled device (CCD). We show that wide-field MIR images can be obtained
at 100 ms exposure times using picosecond pulse energies of only a few fJ per
pixel through NTA directly on the CCD chip. Because this on-chip approach does
not rely on phase-matching, it is alignment-free and does not necessitate
complex post-processing of the images. We emphasize the utility of this
technique through chemically selective MIR imaging of polymers and biological
samples, including MIR videos of moving targets, physical processes and live
nematodes
Tracing the Orphan Stream to 55 kpc with RR Lyrae Stars
We report positions, velocities and metallicities of 50 ab-type RR Lyrae
(RRab) stars observed in the vicinity of the Orphan stellar stream. Using about
30 RRab stars classified as being likely members of the Orphan stream, we study
the metallicity and the spatial extent of the stream. We find that RRab stars
in the Orphan stream have a wide range of metallicities, from -1.5 dex to -2.7
dex. The average metallicity of the stream is -2.1 dex, identical to the value
obtained by Newberg et al. (2010) using blue horizontal branch stars. We find
that the most distant parts of the stream (40-50 kpc from the Sun) are about
0.3 dex more metal-poor than the closer parts (within ~30 kpc), suggesting a
possible metallicity gradient along the stream's length. We have extended the
previous studies and have mapped the stream up to 55 kpc from the Sun. Even
after a careful search, we did not identify any more distant RRab stars that
could plausibly be members of the Orphan stream. If confirmed with other
tracers, this result would indicate a detection of the end of the leading arm
of the stream. We have compared the distances of Orphan stream RRab stars with
the best-fit orbits obtained by Newberg et al. (2010). We find that model 6 of
Newberg et al. (2010) cannot explain the distances of the most remote Orphan
stream RRab stars, and conclude that the best fit to distances of Orphan stream
RRab stars and to the local circular velocity is provided by potentials where
the total mass of the Galaxy within 60 kpc is M_{60}~2.7x10^{11} Msun, or about
60% of the mass found by previous studies. More extensive modelling that would
consider non-spherical potentials and the possibility of misalignment between
the stream and the orbit, is highly encouraged.Comment: Submitted to ApJ, 15 pages in emulateapj format, three tables in
machine-readable format (download "Source" from "Other formats"
Amphidromy Links a Newly Documented Fish Community of Continental Australian Streams, to Oceanic Islands of the West Pacific
BACKGROUND: Indo-Pacific high island streams experience extreme hydrological variation, and are characterised by freshwater fish species with an amphidromous life history. Amphidromy is a likely adaptation for colonisation of island streams following stochastic events that lead to local extirpation. In the Wet Tropics of north-eastern Australia, steep coastal mountain streams share similar physical characteristics to island systems. These streams are poorly surveyed, but may provide suitable habitat for amphidromous species. However, due to their ephemeral nature, common non-diadromous freshwater species of continental Australia are unlikely to persist. Consequently, we hypothesise that coastal Wet Tropics streams are faunally more similar, to distant Pacific island communities, than to nearby faunas of large continental rivers. METHODS/PRINCIPAL FINDINGS: Surveys of coastal Wet Tropics streams recorded 26 species, 10 of which are first records for Australia, with three species undescribed. This fish community is unique in an Australian context in that it contains mostly amphidromous species, including sicydiine gobies of the genera Sicyopterus, Sicyopus, Smilosicyopus and Stiphodon. Species presence/absence data of coastal Wet Tropics streams were compared to both Wet Tropics river networks and Pacific island faunas. ANOSIM indicated the fish fauna of north-eastern Australian coastal streams were more similar to distant Pacific islands (R = 0.76), than to nearby continental rivers (R = 0.98). MAIN CONCLUSIONS/SIGNIFICANCE: Coastal Wet Tropics streams are faunally more similar to distant Pacific islands (79% of species shared), than to nearby continental fauna due to two factors. First, coastal Wet Tropics streams lack many non-diadromous freshwater fish which are common in nearby large rivers. Second, many amphidromous species found in coastal Wet Tropics streams and Indo-Pacific islands remain absent from large rivers of the Wet Tropics. The evolutionary and conservation significance of this newly discovered Australian fauna requires clarification in the context of the wider amphidromous fish community of the Pacific
Amino Acid Repeats Cause Extraordinary Coding Sequence Variation in the Social Amoeba Dictyostelium discoideum
Protein sequences are normally the most conserved elements of genomes owing to purifying selection to maintain their functions. We document an extraordinary amount of within-species protein sequence variation in the model eukaryote Dictyostelium discoideum stemming from triplet DNA repeats coding for long strings of single amino acids. D. discoideum has a very large number of such strings, many of which are polyglutamine repeats, the same sequence that causes various human neurological disorders in humans, like Huntington's disease. We show here that D. discoideum coding repeat loci are highly variable among individuals, making D. discoideum a candidate for the most variable proteome. The coding repeat loci are not significantly less variable than similar non-coding triplet repeats. This pattern is consistent with these amino-acid repeats being largely non-functional sequences evolving primarily by mutation and drift
Costs of formal and informal care at home for people with dementia: ‘expert panel’ opinions from staff and informal carers
Effective home support in dementia is key in delaying nursing home admission. However, home support is frequently not tailored to the individual needs of people with dementia. Staff allocating home support services may not identify important care needs, which only be recognised by informal carers. The purpose of this study was to explore the balance of informal and formal home support and their associated costs from the perspectives of both informal carers and paid staff. Five case vignettes of people with dementia were designed based on an existing English data set from a European study into transition into long-term care (the RightTimePlaceCare programme), representing 42 per cent of the English sample. In total, 14 informal carers and 14 paid staff were consulted in separate groups, as expert panels, regarding their recommendations for home care services for each vignette. Care recommendations of carers and staff were costed based on nationally available unit costs and compared. Informal carers allocated fewer hours of care than staff. Personal and domestic home care and day care centres were the most frequently recommended formal services by both groups, and some vignettes of people with dementia were recommended for care home admission. The ratio of costs of informal versus formal support was relatively equal for paid staff, yet unbalanced from the perspectives of informal carers with a greater proportion of formal care costs. Recommendations from this study can help shape dementia care to be more tailored to the individual needs of people with dementia and their carers
Zwicky Transient Facility constraints on the optical emission from the nearby repeating FRB 180916.J0158+65
The discovery rate of fast radio bursts (FRBs) is increasing dramatically
thanks to new radio facilities. Meanwhile, wide-field instruments such as the
47 deg Zwicky Transient Facility (ZTF) survey the optical sky to study
transient and variable sources. We present serendipitous ZTF observations of
the CHIME repeating source FRB 180916.J0158+65, that was localized to a spiral
galaxy 149 Mpc away and is the first FRB suggesting periodic modulation in its
activity. While 147 ZTF exposures corresponded to expected high-activity
periods of this FRB, no single ZTF exposure was at the same time as a CHIME
detection. No optical source was found at the FRB location in 683
ZTF exposures, totalling 5.69 hours of integration time. We combined ZTF upper
limits and expected repetitions from FRB 180916.J0158+65 in a statistical
framework using a Weibull distribution, agnostic of periodic modulation priors.
The analysis yielded a constraint on the ratio between the optical and radio
fluences of , corresponding to an optical energy erg for a fiducial 10 Jy ms FRB (90%
confidence). A deeper (but less statistically robust) constraint of can be placed assuming a rate of Jy ms)= hr and
FRB occurring during exposures taken in high-activity windows. The
constraint can be improved with shorter per-image exposures and longer
integration time, or observing FRBs at higher Galactic latitudes. This work
demonstrated how current surveys can statistically constrain multi-wavelength
counterparts to FRBs even without deliberately scheduled simultaneous radio
observation.Comment: Accepted for publication in ApJL, 9 pages, 4 figures, 1 tabl
Costs of reproduction are present but latent in eusocial bumblebee queens
Background: The standard evolutionary theory of ageing proposes that ageing occurs because of a trade-off between reproduction and longevity. Eusocial insect queens exhibit positive fecundity-longevity associations and so have been suggested to be counter-examples through not expressing costs of reproduction and through remodelling conserved genetic and endocrine networks regulating ageing and reproduction. If so, eusocial evolution from solitary ancestors with negative fecundity-longevity associations must have involved a stage at which costs of reproduction were suppressed and fecundity and longevity became positively associated. Using the bumblebee (Bombus terrestris), we experimentally tested whether queens in annual eusocial insects at an intermediate level of eusocial complexity experience costs of reproduction, and, using mRNA-seq, the extent to which they exhibit a remodelling of relevant genetic and endocrine networks. Specifically, we tested whether costs of reproduction are present but latent, or whether a remodelling of relevant genetic and endocrine networks has already occurred allowing queens to reproduce without costs. Results: We experimentally increased queens’ costs of reproduction by removing their eggs, which caused queens to increase their egg-laying rate. Treatment queens had significantly reduced longevity relative to control queens whose egg-laying rate was not increased. Reduced longevity in treatment queens was not caused by increased worker-to-queen aggression or by increased overall activity in queens. In addition, treatment and control queens differed in age-related gene expression based on mRNA-seq in both their overall expression profiles and the expression of ageing-related genes. Remarkably, these differences appeared to occur principally with respect to relative age, not chronological age. Conclusions: This study represents the first simultaneously phenotypic and transcriptomic experimental test for a longevity cost of reproduction in eusocial insect queens. The results support the occurrence of costs of reproduction in annual eusocial insects of intermediate social complexity and suggest that reproductive costs are present but latent in queens of such species, i.e. that these queens exhibit condition-dependent positive fecundity-longevity associations. They also raise the possibility that a partial remodelling of genetic and endocrine networks underpinning ageing may have occurred in intermediately eusocial species such that, in unmanipulated conditions, age-related gene expression depends more on chronological than relative age
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