Gestión logística para la Pyme Distribuidora Diana Rocio Prieto del sector Floricultural.

El desarrollo de este proyecto se llevó a cabo con el fin de brindarle instrumentos que permitan realizar una gestión logística acorde a los requerimientos y necesidades, de la microempresa distribuidora Diana Rocío Prieto E.U. Por medio de un plan logístico que pueda lograr un incremento en la optimización de los recursos y mejorar la realización de los procesos. Inicialmente se realizó un diagnóstico del estado actual del sistema logístico de la microempresa, en donde se señalan y definen los actores, recursos, los procesos que se llevan a cabo dentro de la microempresa, el cual da como resultado el esquema logístico de la pyme y el diagrama de flujo de las operaciones que se realizan en la empresa. Posteriormente se aplicó una encuesta a los empleados de la empresa, tanto operarios como administrativos, para la obtención de información relevante que sirviera para la investigación. De esta encuesta, se encontraron las variables críticas del sistema logístico de la empresa, las cuales fueron sometidas a un estudio DOFA, las cuales se complementaron con la definición del esquema logístico y diagrama de operaciones actuales de la empresa. Una vez establecidas las variables críticas del sistema logístico, se realiza la toma de datos para el desarrollo de los indicadores establecidos para evaluar el desempeño de las actividades realizadas por la microempresa. Estos datos históricos se tomaron con un rango de un año. Dentro de los tipos de indicadores tomados para medir el desempeño se encuentran indicadores de productividad, calidad, tiempo y costos. Ya establecidos los actores e indicadores del sistema logístico se procede a la estructuración del plan logístico, que incluye la participación de proveedores, la empresa y clientes. Dentro de este plan se analizan variables de decisión, las cuales son calificadas de acuerdo al desempeño que tienen frente a la empresa. Herramientas como el MRP, planeación agregada, plan maestro logístico, sirvieron para la medición de las áreas de la empresa en donde no se tenían registros de desempeño de actividades, de esta manera se puede ejercer mayor control sobre los procesos de la microempresa Para finalizar todos los datos recogidos por medio de estas herramientas fueron resumidos en un sistema logístico creado en la plataforma de Excel, con la cual la empresa podrá medir su desempeño de manera fácil y ágil.The development of this project was carried out in order to provide tools to perform a logistics management according to the requirements and needs of the enterprise Diana Rocío Prieto E.U. Through a logistics plan that can achieve an increase in the optimizing resources and improving the performance of processes. Initially, it made a diagnosis of the current state of the logistics system in the enterprise to identify and defines the actors, resources, processes that take place inside the company, which results in the scheme and logistics flow diagram of the operations performed in the company. Subsequently it applies a survey to employees of the company, both as administrative workers, to obtain relevant information for research serve. In this survey, we found the critical variables of the logistics system of the company, which were subjected to a SWOT study, and were complemented by the definition of the logistical schema and current diagram business operations. Once established the critical variables of the logistics system, it was made the data collection for the development of the indicators set to evaluate the performance of the activities of microenterprises. These historical data were taken with a range of one year. Among the types of indicators taken to measure performance there are indicators of productivity, quality, time and cost. Once established actors and indicators logistics system, it proceeds to structuring the logistics plan, which includes the participation of suppliers, the company and customers. Within this plan decision variables are analyzed, which are scored according to the performance they have in the company. Tools such as MRP, aggregate planning, logistics master plan, were used to measure the areas of the company where they had no records of performance of activities, so it can have more control over the processes of the enterprise. To finish, all the data collected by these tools were summarized in a logistics system platform created in Excel, with which the company can measure its performance easily and fast

Time course of early metabolic changes following diffuse traumatic brain injury in rats as detected by 1H NMR spectroscopy

Experimental models of traumatic brain injury (TBI) provide a useful tool for understanding the cerebral metabolic changes induced by this pathological condition. Here, we report on the time course of changes in cerebral metabolites after TBI and its correlation with early brain morphological changes using a combination of high-resolution proton magnetic resonance spectroscopy ( 1H MRS) and magnetic resonance imaging (MRI). Adult male Sprague-Dawley rats were subjected to closed head impact and examined by MRI at 1, 9, 24, 48, and and 72 h after the injury. Extracts from funnel frozen rat brains were then obtained and analyzed quantitatively by high-resolution 1H MRS. Finally, statistical multivariate analysis was carried out to identify the combination of cerebral metabolites that best described the time evolution of diffuse TBI. The temporal changes observed in the concentration of cerebral metabolites followed three different patterns. The first pattern included taurine, threonine, and glycine, with concentrations peaking 24 h after the injury. The second pattern included glutamate, GABA, and alanine, with concentrations remaining elevated between 24 and 48 h post-injury. The third one involved creatine-phosphocreatine, N-acetylaspartate, and myo-inositol, with concentrations peaking 48 h after the injury. A multivariate stepwise discriminant analysis revealed that the combination of the organic osmolytes taurine and myo-inositol allowed optimal discrimination among the different time groups. Our findings suggest that the profile of some specific brain molecules that play a role as organic osmolytes can be used to follow-up the progression of the early diffuse brain edema response induced by TBI. © Mary Ann Liebert, Inc.This work was partly supported by Spanish Ministry of Education and Science (grants SAF 2001-224 and SAF 2004-03197 to J.M.R. and S.C.) and by Spanish Ministry of Health (grants FISss C03/08, C03/10, and G03/155 to J.M.R. and S.C.).Peer Reviewe

Sistemas dinámicos cardiacos en neonatos normales: Ley caótica cardiaca neonatal

Objetivo: caracterizar matemáticamente la dinámica cardiaca neonatal normal en cuatro estados: dormido quieto, dormido activo, despierto quieto y despierto activo, para desarrollar una generalización determinando todos los posibles atractores normales. Materiales y Métodos: Con base en la teoría de los sistemas dinámicos, se tomaron los intervalos RR mínimos y máximos de un neonato en cuatro estados comportamentales. Se realizó una simulación de la frecuencia de la dinámica cardiaca para cada estado construyendo atractores caóticos. Posteriormente se cuantificaron los espacios de ocupación y las dimensiones fractales de los atractores en el espacio generalizado de Box Counting, buscando igualdades y diferencias entre estos estados dinámicos. Finalmente se calcularon todos los posibles atractores normales, basado en una ley exponencial desarrollada previamente para la dinámica cardiaca de adultos. Resultados: Se encontraron diferencias entre los cuatro estados comportamentales, al comparar medidas de ocupación espacial, las cuales presentaron valores de 199 para S1, 131 para S2, 61 para S3 y 175 para S4 en la rejilla Kp. Así mismo se encontraron valores de dimensión fractal diferenciadores para cada uno de los estados. El número de atractores normales totales fue de 4602. Conclusión: Se desarrolló una nueva metodología físico- matemática acausal de la dinámica cardiaca neonatal, que permite la diferenciación de diferentes estados comportamentales y el establecimiento de la totalidad de dinámicas normales

Surgical treatment for recurrent cholangiocarcinoma: a single-center series

Purpose: The present study aims to assess the results obtained after surgical treatment of cholangiocarcinoma (CC) recurrences. Methods: We carried out a single-center retrospective study, including all patients with recurrence of CC. The primary outcome was patient survival after surgical treatment compared with chemotherapy or best supportive care. A multivariate analysis of variables affecting mortality after CC recurrence was performed. Results: Eighteen patients were indicated surgery to treat CC recurrence. Severe postoperative complication rate was 27.8% with a 30-day mortality rate of 16.7%. Median survival after surgery was 15 months (range 0-50) with 1- and 3-year patient survival rates of 55.6% and 16.6%, respectively. Patient survival after surgery or CHT alone, was significantly better than receiving supportive care (p< 0.001). We found no significant difference in survival when comparing CHT alone and surgical treatment (p=0.113). Time to recurrence of <1 year, adjuvant CHT after resection of the primary tumor and undergoing surgery or CHT alone versus best supportive care were independent factors affecting mortality after CC recurrence in the multivariate analysis. Conclusion: Surgery or CHT alone improved patient survival after CC recurrence compared to best supportive care. Surgical treatment did not improve patient survival compared to CHT alone

Investigaciones arqueométricas en las cuevas de Ardales y Las Palomas de Teba (Comarca del Guadalteba, Málaga)

We have performed a Raman miscroscopic analysis of a series of prehistoric artifacs found inside the Ardales Cave (Ardales, Málaga, Spain), such as a number of fixed and portable fat-burning stone lamps, some lithic tools and wall paintings, by means of either a portable Raman spectrometer for in situ studies in the field or bench-top Raman spectrometers and Raman microscopes in the laboratory of Vibrational Spectroscopy at the Central Building of Scientific Facilities of the University of Málaga (SCAI, UMA).We have performed a Raman miscroscopic analysis of a series of prehistoric artifacs found inside the Ardales Cave (Ardales, Málaga, Spain), such as a number of fixed and portable fat-burning stone lamps, some lithic tools and wall paintings, by means of either a portable Raman spectrometer for in situ studies in the field or bench-top Raman spectrometers and Raman microscopes in the laboratory of Vibrational Spectroscopy at the Central Building of Scientific Facilities of the University of Málaga (SCAI, UMA).Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

A Comparative Study of Cell Culture Conditions during Conversion from Primed to Naive Human Pluripotent Stem Cells

The successful reprogramming of human somatic cells into induced pluripotent stem cells (hiPSCs) represented a turning point in the stem cell research field, owing to their ability to differentiate into any cell type with fewer ethical issues than human embryonic stem cells (hESCs). In mice, PSCs are thought to exist in a naive state, the cell culture equivalent of the immature pre-implantation embryo, whereas in humans, PSCs are in a primed state, which is a more committed pluripotent state than a naive state. Recent studies have focused on capturing a similar cell stage in human cells. Given their earlier developmental stage and therefore lack of cell-of-origin epigenetic memory, these cells would be better candidates for further re-differentiation, use in disease modeling, regenerative medicine and drug discovery. In this study, we used primed hiPSCs and hESCs to evaluate the successful establishment and maintenance of a naive cell stage using three different naive-conversion media, both in the feeder and feeder-free cells conditions. In addition, we compared the directed differentiation capacity of primed and naive cells into the three germ layers and characterized these different cell stages with commonly used pluripotent and lineage-specific markers. Our results show that, in general, naive culture NHSM medium (in both feeder and feeder-free systems) confers greater hiPSCs and hESCs viability and the highest naive pluripotency markers expression. This medium also allows better cell differentiation cells toward endoderm and mesoderm.This work was supported by the Health Department of the Basque Government (Grant 2019111068, 2019/4703, 2020111058, 2020333032, 2021333057 and 2021333012), Merck-Salud Founda- tion (FSALUD17/004), Economic Development and Infrastructures Department of the Basque Govern- ment (KK-2020/00068), EITB Maratoia (BIO21/COV/030), Project “PI18/01299” and “PI21/01187”, funded by Instituto de Salud Carlos III and co-funded by European Union (ERDF) “A way to make Europe”, “ICI21/00095” funded by Instituto de Salud Carlos III and co-funded by European Union (NextGenerationEU), “Plan de Recuperación Transformación y Resiliencia” Investigación Clínica Independiente 2021–Acción Estratégica Salud 2017–2020, RICORS: (RD21/00017/0024) Red Española de Terapias Avanzadas TERAV ISCIII. Funded by Instituto de Salud Carlos III (ISCIII) and co-funded by European Union (NextGenerationEU) “Plan de Recuperación Transformación y Resiliencia” Redes de Investigación Cooperativa Orientadas a Resultados en Salud (RICORS) 2021–Acción Estratégica Salud 2017–2020. L.H. was supported by the Jesus Gangoiti Barrera Foundation and the Asociación Española contra el Cáncer (AECC) AECC16/501 and the Fundación Mutua Madrileña AP176182020. M.M-I was supported by Jesus Gangoiti Barrera Foundation. I.R was supported by Margarita Salas Grant “MARSA21/60” and the Jesus Gangoiti Barrera Foundation. M.I-F. was supported by Inocente Inocente Foundation FII18/003. J.R.P. has grant “RYC-2013-13450” funded by MCIN/AEI/10.13039/501100011033, by the European Social Fund “ESF investing in your future”

Activation of p21 limits acute lung injury and induces early senescence after acid aspiration and mechanical ventilation

The p53/p21 pathway is activated in response to cell stress. However, its role in acute lung injury has not been elucidated. Acute lung injury is associated with disruption of the alveolo-capillary barrier leading to acute respiratory distress syndrome (ARDS). Mechanical ventilation may be necessary to support gas exchange in patients with ARDS, however, high positive airway pressures can cause regional overdistension of alveolar units and aggravate lung injury. Here, we report that acute lung injury and alveolar overstretching activate the p53/p21 pathway to maintain homeostasis and avoid massive cell apoptosis. A systematic pooling of transcriptomic data from animal models of lung injury demonstrates the enrichment of specific p53- and p21-dependent gene signatures and a validated senescence profile. In a clinically relevant, murine model of acid aspiration and mechanical ventilation, we observed changes in the nuclear envelope and the underlying chromatin, DNA damage and activation of the Tp53/p21 pathway. Absence of Cdkn1a decreased the senescent response, but worsened lung injury due to increased cell apoptosis. Conversely, treatment with lopinavir/ritonavir led to Cdkn1a overexpression and ameliorated cell apoptosis and lung injury. The activation of these mechanisms was associated with early markers of senescence, including expression of senescence-related genes and increases in senescence-associated heterochromatin foci in alveolar cells. Autopsy samples from lungs of patients with ARDS revealed increased senescence-associated heterochromatin foci. Collectively, these results suggest that acute lung injury activates p53/p21 as an anti-apoptotic mechanism to ameliorate damage, but with the side effect of induction of senescence

HIV-reservoir size is not affected either by HCV coinfection or by direct acting antivirals (DAAs) therapy

The role of HCV on the HIV reservoir is controversial since the reduction on HIV-DNA levels after HCV eradication with IFNα/RBV treatment seems to be the result of drugs instead of HCV clearance. We assessed whether HCV eradication can decrease HIV-DNA content in HIV/HCV-coinfected patients treated with direct-acting antivirals, DAAs (IFNα/RBV-free regimens). Cell-associated HIV-DNA was measured by ddPCR in 25 HIV-monoinfected and 25 HIV/HCV-coinfected patients. There were no differences in HIV-DNA levels between groups neither at baseline nor at 12 weeks after DAAs treatment completion. Our results indicate that HCV does not appear to influence the HIV reservoir size and suggest the lack of an anti-HIV action for DAAs.This work was supported by projects PI14/00518, RD16/0025/0013 integrated into the State Plan for Scientific and Technical Research and Innovation from the General Sub-Directorate for research assessment and promotion, Spanish Carlos III Institute of Health (ISCIII) co-funded by the European Regional Development Fund (ERDF). Maria A Navarrete-Muñoz was funded by the Spanish Directorate General for Research and Technological of the “Comunidad de Madrid” [grant: IND2018/BMD9651]. Norma Rallón is supported by the Miguel Servet program funded by the Spanish Health Institute Carlos III [grant: CPII19/00025].S

The young Millennial generation and their socially responsible consumption in Bogotá, Colombia

El presente trabajo es un avance de una investigación titulada “Medición y caracterización del consumo socialmente responsable en Colombia”. El objetivo de este documento consiste en exponer el panorama general sobre el significado y la actitud que tienen los bogotanos de estratos socioeconómicos 3 y 4, frente al tema del consumo socialmente responsable; de acuerdo con las estrategias de comunicación desarrolladas en pro de una actitud positiva con la temática abordada, en jóvenes de 18- 25 años “Millennials”, de la ciudad de Bogotá. En cuanto a la metodología implementada, se realizó una investigación exploratoria, con alcance cualitativo; para el trabajo de campo se desarrolló una sesión de grupo como técnica directa para finalmente analizar la información recopilada con el software Atlas ti.This paper is an advance of a research entitled “Measurement and characterization of socially responsible consumption in Colombia". The purpose of this paper is to present an overview of the meaning and attitude of bogotanos from socioeconomic strata 3 and 4 on the issue of socially responsible consumption; according to the communication strategies developed towards a positive attitude to the topics addressed at young people aged 18-25 "Millennials" of the city of Bogota. Regarding the methodology implemented, an exploratory research was conducted with a qualitative range; for fieldwork, a group session as a direct technique was conducted to finally analyze the information collected with the Atlas ti software