Los emigrados políticos españoles en la emergencia de la cuestión obrera argentina. Rosario 1895-1904

En los años del tránsito del siglo XIX al XX Argentina fue tierra de acogida de una porción sustantiva de la emigración política española. Algunos de ellos ocuparon lugares expectantes en el seno de las élites dominantes y se destacaron en la escena intelectual y política. Este trabajo analiza la incidencia que tuvieron la obra y las intervenciones públicas de Serafín Alvarez, Juan Bialet Massé y Emilio L. Rodríguez en el proceso de emergencia de la cuestión obrera argentina.Between the end of the XIX century and the beginning of the XX, a large proportion of spanish political emigrants arrived to Argentina. Some of them achieved taking part in the elite and they stood out in the political and intellectual stage. This paper analyses the incidence of the work and public interventions of Serafín Alvarez, Juan Bialet Massé y Emilio L. Rodríguez in the emergency process of the argentine labor question

Legados simbólicos de las primeras experiencias de conflicto social

Esta presentación analiza las proyecciones prácticas y simbólicas que tuvieron dos acontecimientos que compartieron escenario, la ciudad de Rosario, y el hecho de no haber tenido precedentes nacionales: la huelga general de 1896 y la muerte de un huelguista a manos de la policía, en octubre de 1901. De las proyecciones, mayormente locales, de la huelga grande de 1896 da cuenta su sensible impacto social, político e institucional. De las ideológicas, Los trabajadores en la Argentina, obra fundacional de Adrián Patroni que, al atribuir el fracaso del conflicto gremial de origen al carácter generalizado de la huelga rosarina, pone en locución los términos de la ríspida controversia sobre los medios que mantendrán las organizaciones obreras en los años venideros. Las proyecciones de la caída del primer mártir obrero fueron, ante todo, simbólicas. El antagonismo social adquirió carnadura dramática y se delinearon, en el plano de las tradiciones, los primeros trazos de una perdurable ritualidad de la muerte.Facultad de Humanidades y Ciencias de la Educació

Los emigrados políticos españoles en la emergencia de la cuestión obrera argentina. Rosario 1895-1904

En los años del tránsito del siglo XIX al XX Argentina fue tierra de acogida de una porción sustantiva de la emigración política española. Algunos de ellos ocuparon lugares expectantes en el seno de las élites dominantes y se destacaron en la escena intelectual y política. Este trabajo analiza la incidencia que tuvieron la obra y las intervenciones públicas de Serafín Alvarez, Juan Bialet Massé y Emilio L. Rodríguez en el proceso de emergencia de la cuestión obrera argentina.Between the end of the XIX century and the beginning of the XX, a large proportion of spanish political emigrants arrived to Argentina. Some of them achieved taking part in the elite and they stood out in the political and intellectual stage. This paper analyses the incidence of the work and public interventions of Serafín Alvarez, Juan Bialet Massé y Emilio L. Rodríguez in the emergency process of the argentine labor question

Multiscale modeling of prismatic heterogeneous structures based on a localized hyperreduced-order method

This work aims at deriving special types of one-dimensional Finite Elements (1D FE) for efficiently modeling heterogeneous prismatic structures, in the small strains regime, by means of reduced-order modeling (ROM) and domain decomposition techniques. The employed partitioning framework introduces “fictitious” interfaces between contiguous subdomains, leading to a formulation with both subdomain and interface fields. We propose a low-dimensional parameterization at both subdomain and interface levels by using reduced-order bases precomputed in an offline stage by applying the Singular Value Decomposition (SVD) on solution snapshots. In this parameterization, the amplitude of the fictitious interfaces play the role of coarse-scale displacement unknowns. We demonstrate that, with this partitioned framework, it is possible to arrive at a solution strategy that avoids solving the typical nested local/global problem of other similar methods (such as the FE method). Rather, in our approach, the coarse-grid cells can be regarded as special types of finite elements, whose nodes coincides with the centroids of the interfaces, and whose kinematics are dictated by the modes of the “fictitious” interfaces. This means that the kinematics of our coarse-scale FE are not pre-defined by the user, but extracted from the set of “training” computational experiments. Likewise, we demonstrate that the coarse-scale and fine-scale displacements are related by inter-scale operators that can be precomputed in the offline stage. Lastly, a hyperreduced scheme is considered for the evaluation of the internal forces, allowing us to deal with possible material nonlinearities.This work has received support from the Spanish Ministry of Economy and Competitiveness, through the “Severo Ochoa Programme for Centres of Excellence in R&D” (CEX2018-000797-S)”. A. Giuliodori also gratefully acknowledges the support of “Secretaria d’Universitats i Recerca de la Generalitat de Catalunya i del Fons Social Europeu” through the FI grant (00939/2020), and J.A. Hernández the support of, on the one hand, the European High-Performance Computing Joint Undertaking (JU) under grant agreement No. 955558 (the JU receives, in turn, support from the European Union’s Horizon 2020 research and innovation program and from Spain, Germany, France, Italy, Poland, Switzerland, Norway), and the European Union’s Horizon 2020 research and innovation program under Grant Agreement No. 952966 (project FIBREGY).Peer ReviewedPostprint (published version

Escritos de Virginia Bolten

Escritos de Virginia Bolten reunidos, editados y publicados en el contexto del presente Dossier

Tras los pasos de Virginia Bolten

Este trabajo es una biografía política e intelectual de Virginia Bolten, una de las anarquistas más reconocidas en la historia del movimiento obrero de Argentina y Uruguay. Tanto en la memoria militante como en el relato historiográfico, la vida de Bolten surge como un conjunto de imágenes poderosas e insistentes. La audaz que arengó a las masas en 1890. La feminista redomada que concibió el periódico La Voz de la Mujer. La joven llamativa que descubrió Bialet Massé en su informe de 1904. La militante mujer detenida y deportada. Sobre esas imágenes sueltas trabajaron los autores de este artículo, con el objetivo de revisarlas y establecer puntos de referencia para dar cuenta de los tramos más significativos de la actuación de Bolten en la primera parte de su vida.  Para esta edición de Políticas de la Memoria han recuperado veinte intervenciones de Bolten en la prensa durante las primeras décadas de su militancia. Se incluyen, también, algunas fotos provenientes del archivo familiar y el acta de bautismo de 1880

Screening of CHO-K1 endogenous promoters for expressing recombinant proteins in mammalian cell cultures

For the production of recombinant protein therapeutics in mammalian cells, a high rate of gene expression is desired and hence strong viral-derived promoters are commonly used. However, they usually induce cellular stress and can be susceptible to epigenetic silencing. Endogenous promoters, which coordinates their activity with cellular and bioprocess dynamics while at the same time they maintain high expression levels, may help to avoid such drawbacks. In this work, new endogenous promoters were discovered based on high expression levels in RNA-seq data of CHO-K1 cells cultured in high density. The promoters of Actb, Ctsz, Hmox1, Hspa5, Vim and Rps18 genes were selected for generating new expression vectors for the production of recombinant proteins in mammalian cells. The in silico-derived promoter regions were experimentally verified and the majority showed transcriptional activity comparable or higher than CMV. Also, stable expression following a reduction of culture temperature was investigated. The characterized endogenous promoters (excluding Rps18) constitute a promising alternative to CMV promoter due to their high strength, long-term expression stability and integration into the regulatory network of the host cell. These promoters may also comprise an initial panel for designing cell engineering strategies and synthetic promoters, as well as for industrial cell line development.Fil: Tossolini, Ileana del Rosario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Gugliotta, Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: López Díaz, Fernando. Salk Institute for Biological Studies; Estados UnidosFil: Kratje, Ricardo Bertoldo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Prieto, Claudio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentin

Exopolysaccharide-producing Lacticaseibacillus paracasei strains isolated from kefir as starter for functional dairy products

Exopolysaccharides (EPS) produced by lactic acid bacteria are molecules of great interest for the dairy food industry. Lacticaseibacillus paracasei CIDCA 8339, CIDCA 83123, and CIDCA 83124 are potentially probiotic strains isolated from kefir grains whose EPS-production on MRS broth is dependent on incubation temperature. The aim of the present work is to evaluate the effect of fermentation temperature on the characteristics of EPS produced in milk by L. paracasei strains and the consequent impact on the rheological properties of the fermented products. Additionally, the protective effect of these EPS against Salmonella infection was evaluated in vitro. Acid gels with each strain were obtained by milk fermentation at 20°C, 30°C, and 37°C evidencing for all the strains a reduction in growth and acidification rate at lower temperature. Lacticaseibacillus paracasei CIDCA 83123 showed low fermentation rate at all temperatures requiring between 3 and 8 days to obtain acids gels, whereas CIDCA 8339 and 83124 needed between 24 and 48 h even when the temperature was 20°C. Fermentation temperature led to changes in crude EPS characteristics of the three strains, observing an increase in the relative amount of the high molecular weight fraction when the fermentation temperature diminished. Additionally, EPS83124 and EPS83123 presented modifications in monosaccharide composition, with a reduction of rhamnose and an increase of amino-sugars as temperature rise. These changes in the structure of EPS83124 resulted in an increase of the apparent viscosity of milks fermented at 20°C (223 mPa.s) and 30°C (217 mPa.s) with respect to acid gels obtained at 37°C (167 mPa.s). In order to deepen the knowledge on EPS characteristics, monosaccharide composition of low and high molecular weight EPS fractions were evaluated. Finally, it was evidenced that the preincubation of intestinal epithelial cells Caco-2/TC-7 with EPS8339 and EPS83124 partially inhibit the association and invasion of Salmonella. In light of these results, it can be concluded that the selection of the EPS-producing strain along with the appropriate fermentation conditions could be an interesting strategy to improve the technological properties of these L. paracasei fermented milks with potential protective effects against intestinal pathogens.The present work was supported by CONICET (PIP 2786), Universidad Nacional de La Plata (UNLP 18/X813), ANPCyT (PICT 2020-03973 and PICT 2020-3239), and the Basque Government (IT1662-22 and PIBA 2020_1_0032)

Numerical modeling of bare and polymer-covered braided stents using torsional and tensile springs connectors

Computational modeling of braided stents using the finite element (FE) method has become an essential tool in the design and development of these medical devices. One of the most challenging issues in such a task is representing in an accurate manner the interaction between the interlacing wires. With the goal of achieving a compromise between accuracy and computational affordability, we propose a new approach consisting in using 1D FE formulations equipped with torsional springs at the crossover points of the wires. In the case of covered braided stents, the model is enriched with a set of tensile springs (defined in the longitudinal direction), aimed at capturing the stiffening effect of the polymeric membrane. The predictive capabilities of the proposed model are evaluated using data of our own experimental tests, as well as data from other tests in the literature. The simulations demonstrate that the proposed model is able to predict the (markedly nonlinear) behavior of stents when subjected to radial and axial cycle loads, with errors at the end of the compression stage ranging from 0.5% to 10% in all cases.The research leading to these results has received funding from ANACONDA BIOMED S.L., Spain and the Spanish Ministry of Science, Innovation and Universities via grant RTC-2017–6749-1. The authors also acknowledges financial support from the Spanish Ministry of Economy and Competitiveness, through the ”Severo Ochoa Programme for Centres of Excellence in RD” (CEX2018-000797-S) and EMPLEA(EMP-TU-2016–5296), from CDTI (SNEO: 20161073), from ACCIÓ (NUCLIS RD17-1–0086) and from EIT Health (2018 HS PoC 2018-HS-0170). A.Giuliodori gratefully acknowledges as well the support of Secretaria d’Universitats i Recerca de la Generalitat de Catalunya i del Fons Social Europeu through the FI grant (00939/2020).Peer ReviewedPostprint (author's final draft

Biological response and developmental toxicity of zebrafish embryo and larvae exposed to multi-walled carbon nanotubes with different dimension

The development and use of nanomaterials are increasing significantly. Among nanomaterials, carbon nanotubes are of particular interest due to its distinctive physicochemical properties. This material composed of sheets of graphite has very high thermal conductivity, metallic-type electrical conductivity, stiffness, toughness and unique ability to bond to itself in an extended network with extraordinary strength. Its application in the industry is continuously growing, which could lead to the accumulation in the environment and a consequent impact on both humans and ecosystems. Considering that environmental systems are dynamic, it is difficult to predict the risks associated with the release of nanomaterials to the environment. Bioindicators are useful tools as primary signals of environmental risk, and their responses reveal the organism and ecosystem health. In the present study, we evaluated the impact of multi-walled carbon nanotubes with different dimensions and agglomeration pattern on zebrafish embryo and larvae; mainly, studies were focused on physiological and behavioral responses. In embryos, measurements were hatching rate, morphology changes, and viability. In larvae, locomotor activity, heart rate, innate inflammatory response, general and tissue-specific morphology were measured. MWCNT-S (short, wide and mostly dispersed) caused depression of the locomotor activity of larvae, indicating an alteration of the central nervous system, and depression of neutrophil migration activity. MWCNT-L (long, thin and agglomerated) caused malformations during larval development, a decrease of neutrophil migration and alteration of cardiac rhythm. Results obtained for both carbon nanotubes were different, highlighting the importance of dimensions of the same nanomaterial, and also the kind of agglomeration and shape adopted, for the toxic effects on organisms.Fil: Martinez, Carolina Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Grupo Vinculado al IMBICE - Grupo de Biología Estructural y Biotecnología - Universidad Nacional de Quilmes - GBEyB | Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Grupo Vinculado al IMBICE - Grupo de Biología Estructural y Biotecnología - Universidad Nacional de Quilmes - GBEyB | Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular. Grupo Vinculado al IMBICE - Grupo de Biología Estructural y Biotecnología - Universidad Nacional de Quilmes - GBEyB; ArgentinaFil: Igartúa, Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Grupo Vinculado al IMBICE - Grupo de Biología Estructural y Biotecnología - Universidad Nacional de Quilmes - GBEyB | Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Grupo Vinculado al IMBICE - Grupo de Biología Estructural y Biotecnología - Universidad Nacional de Quilmes - GBEyB | Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular. Grupo Vinculado al IMBICE - Grupo de Biología Estructural y Biotecnología - Universidad Nacional de Quilmes - GBEyB; ArgentinaFil: Czarnowski, Ian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Grupo Vinculado al IMBICE - Grupo de Biología Estructural y Biotecnología - Universidad Nacional de Quilmes - GBEyB | Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Grupo Vinculado al IMBICE - Grupo de Biología Estructural y Biotecnología - Universidad Nacional de Quilmes - GBEyB | Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular. Grupo Vinculado al IMBICE - Grupo de Biología Estructural y Biotecnología - Universidad Nacional de Quilmes - GBEyB; ArgentinaFil: Feas, Daniela Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Grupo Vinculado al IMBICE - Grupo de Biología Estructural y Biotecnología - Universidad Nacional de Quilmes - GBEyB | Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Grupo Vinculado al IMBICE - Grupo de Biología Estructural y Biotecnología - Universidad Nacional de Quilmes - GBEyB | Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular. Grupo Vinculado al IMBICE - Grupo de Biología Estructural y Biotecnología - Universidad Nacional de Quilmes - GBEyB; ArgentinaFil: Alonso, Silvia del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Grupo Vinculado al IMBICE - Grupo de Biología Estructural y Biotecnología - Universidad Nacional de Quilmes - GBEyB | Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Grupo Vinculado al IMBICE - Grupo de Biología Estructural y Biotecnología - Universidad Nacional de Quilmes - GBEyB | Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular. Grupo Vinculado al IMBICE - Grupo de Biología Estructural y Biotecnología - Universidad Nacional de Quilmes - GBEyB; ArgentinaFil: Prieto, Maria Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Grupo Vinculado al IMBICE - Grupo de Biología Estructural y Biotecnología - Universidad Nacional de Quilmes - GBEyB | Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Grupo Vinculado al IMBICE - Grupo de Biología Estructural y Biotecnología - Universidad Nacional de Quilmes - GBEyB | Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular. Grupo Vinculado al IMBICE - Grupo de Biología Estructural y Biotecnología - Universidad Nacional de Quilmes - GBEyB; Argentin