307 research outputs found
Magnetic and structural properties of GeMn films: precipitation of intermetallic nanomagnets
We present a comprehensive study relating the nanostructure of Ge_0.95Mn_0.05
films to their magnetic properties. The formation of ferromagnetic nanometer
sized inclusions in a defect free Ge matrix fabricated by low temperature
molecular beam epitaxy is observed down to substrate temperatures T_S as low as
70 deg. Celsius. A combined transmission electron microscopy (TEM) and electron
energy-loss spectroscopy (EELS) analysis of the films identifies the inclusions
as precipitates of the ferromagnetic compound Mn_5Ge_3. The volume and amount
of these precipitates decreases with decreasing T_S. Magnetometry of the films
containing precipitates reveals distinct temperature ranges: Between the
characteristic ferromagnetic transition temperature of Mn_5Ge_3 at
approximately room temperature and a lower, T_S dependent blocking temperature
T_B the magnetic properties are dominated by superparamagnetism of the Mn_5Ge_3
precipitates. Below T_B, the magnetic signature of ferromagnetic precipitates
with blocked magnetic moments is observed. At the lowest temperatures, the
films show features characteristic for a metastable state.Comment: accepted for publication in Phys. Rev. B 74 (01.12.2006). High
resolution images ibide
Effects of Electron-Electron Scattering on Electron-Beam Propagation in a Two-Dimensional Electron-Gas
We have studied experimentally and theoretically the influence of
electron-electron collisions on the propagation of electron beams in a
two-dimensional electron gas for excess injection energies ranging from zero up
to the Fermi energy. We find that the detector signal consists of
quasiballistic electrons, which either have not undergone any electron-electron
collisions or have only been scattered at small angles. Theoretically, the
small-angle scattering exhibits distinct features that can be traced back to
the reduced dimensionality of the electron system. A number of nonlinear
effects, also related to the two-dimensional character of the system, are
discussed. In the simplest situation, the heating of the electron gas by the
high-energy part of the beam leads to a weakening of the signal of
quasiballistic electrons and to the appearance of thermovoltage. This results
in a nonmonotonic dependence of the detector signal on the intensity of the
injected beam, as observed experimentally.Comment: 9 pages, 7 figure
Microscopic View on Short-Range Wetting at the Free Surface of the Binary Metallic Liquid Gallium-Bismuth: An X-ray Reflectivity and Square Gradient Theory Study
We present an x-ray reflectivity study of wetting at the free surface of the
binary liquid metal gallium-bismuth (Ga-Bi) in the region where the bulk phase
separates into Bi-rich and Ga-rich liquid phases. The measurements reveal the
evolution of the microscopic structure of wetting films of the Bi-rich,
low-surface-tension phase along different paths in the bulk phase diagram. A
balance between the surface potential preferring the Bi-rich phase and the
gravitational potential which favors the Ga-rich phase at the surface pins the
interface of the two demixed liquid metallic phases close to the free surface.
This enables us to resolve it on an Angstrom level and to apply a mean-field,
square gradient model extended by thermally activated capillary waves as
dominant thermal fluctuations. The sole free parameter of the gradient model,
i.e. the so-called influence parameter, , is determined from our
measurements. Relying on a calculation of the liquid/liquid interfacial tension
that makes it possible to distinguish between intrinsic and capillary wave
contributions to the interfacial structure we estimate that fluctuations affect
the observed short-range, complete wetting phenomena only marginally. A
critical wetting transition that should be sensitive to thermal fluctuations
seems to be absent in this binary metallic alloy.Comment: RevTex4, twocolumn, 15 pages, 10 figure
Intrinsic phase separation in superconducting K0.8Fe1.6Se2 (Tc= 31.8 K) single crystals
Temperature dependent single-crystal x-ray diffraction (XRD) in transmission
mode probing the bulk of the newly discovered K0.8Fe1.6Se2 superconductor (Tc =
31.8 K) using synchrotron radiation is reported. A clear evidence of intrinsic
phase separation at 520 K between two competing phases, (i) a first majority
magnetic phase with a ThCr2Si2-type tetragonal lattice modulated by the iron
vacancy ordering and (ii) a minority non-magnetic phase having an in-plane
compressed lattice volume and a weak superstructure, is reported. The XRD peaks
due to the Fe vacancy ordering in the majority phase disappear by increasing
the temperature at 580 K, well above phase separation temperature confirming
the order-disorder phase transition. The intrinsic phase separation at 520K
between a competing first magnetic phase and a second non-magnetic phase in the
normal phase both having lattice superstructures (that imply different Fermi
surface topology reconstruction and charge density) is assigned to a
lattice-electronic instability of the K0.8Fe1.6Se2 system typical of a system
tuned at a Lifshitz critical point of an electronic topological transition that
gives a multi-gaps superconductor tuned a shape resonance.Comment: 10 pages, 4 figure
Unique features of a global human ectoparasite identified through sequencing of the bed bug genome
The bed bug, Cimex lectularius, has re-established itself as a ubiquitous
human ectoparasite throughout much of the world during the past two decades.
This global resurgence is likely linked to increased international travel and
commerce in addition to widespread insecticide resistance. Analyses of the C.
lectularius sequenced genome (650âMb) and 14,220 predicted protein-coding
genes provide a comprehensive representation of genes that are linked to
traumatic insemination, a reduced chemosensory repertoire of genes related to
obligate hematophagy, hostâsymbiont interactions, and several mechanisms of
insecticide resistance. In addition, we document the presence of multiple
putative lateral gene transfer events. Genome sequencing and annotation
establish a solid foundation for future research on mechanisms of insecticide
resistance, humanâbed bug and symbiontâbed bug associations, and unique
features of bed bug biology that contribute to the unprecedented success of C.
lectularius as a human ectoparasite
A large population of diverse neurons in the Drosophila central nervous system expresses short neuropeptide F, suggesting multiple distributed peptide functions
<p>Abstract</p> <p>Background</p> <p>Insect neuropeptides are distributed in stereotypic sets of neurons that commonly constitute a small fraction of the total number of neurons. However, some neuropeptide genes are expressed in larger numbers of neurons of diverse types suggesting that they are involved in a greater diversity of functions. One of these widely expressed genes, <it>snpf</it>, encodes the precursor of short neuropeptide F (sNPF). To unravel possible functional diversity we have mapped the distribution of transcript of the <it>snpf </it>gene and its peptide products in the central nervous system (CNS) of <it>Drosophila </it>in relation to other neuronal markers.</p> <p>Results</p> <p>There are several hundreds of neurons in the larval CNS and several thousands in the adult <it>Drosophila </it>brain expressing <it>snpf </it>transcript and sNPF peptide. Most of these neurons are intrinsic interneurons of the mushroom bodies. Additionally, sNPF is expressed in numerous small interneurons of the CNS, olfactory receptor neurons (ORNs) of the antennae, and in a small set of possibly neurosecretory cells innervating the corpora cardiaca and aorta. A sNPF-Gal4 line confirms most of the expression pattern. None of the sNPF immunoreactive neurons co-express a marker for the transcription factor DIMMED, suggesting that the majority are not neurosecretory cells or large interneurons involved in episodic bulk transmission. Instead a portion of the sNPF producing neurons co-express markers for classical neurotransmitters such as acetylcholine, GABA and glutamate, suggesting that sNPF is a co-transmitter or local neuromodulator in ORNs and many interneurons. Interestingly, sNPF is coexpressed both with presumed excitatory and inhibitory neurotransmitters. A few sNPF expressing neurons in the brain colocalize the peptide corazonin and a pair of dorsal neurons in the first abdominal neuromere coexpresses sNPF and insulin-like peptide 7 (ILP7).</p> <p>Conclusion</p> <p>It is likely that sNPF has multiple functions as neurohormone as well as local neuromodulator/co-transmitter in various CNS circuits, including olfactory circuits both at the level of the first synapse and at the mushroom body output level. Some of the sNPF immunoreactive axons terminate in close proximity to neurosecretory cells producing ILPs and adipokinetic hormone, indicating that sNPF also might regulate hormone production or release.</p
Neuroarchitecture of Aminergic Systems in the Larval Ventral Ganglion of Drosophila melanogaster
Biogenic amines are important signaling molecules in the central nervous system of both vertebrates and invertebrates. In the fruit fly Drosophila melanogaster, biogenic amines take part in the regulation of various vital physiological processes such as feeding, learning/memory, locomotion, sexual behavior, and sleep/arousal. Consequently, several morphological studies have analyzed the distribution of aminergic neurons in the CNS. Previous descriptions, however, did not determine the exact spatial location of aminergic neurite arborizations within the neuropil. The release sites and pre-/postsynaptic compartments of aminergic neurons also remained largely unidentified. We here used gal4-driven marker gene expression and immunocytochemistry to map presumed serotonergic (5-HT), dopaminergic, and tyraminergic/octopaminergic neurons in the thoracic and abdominal neuromeres of the Drosophila larval ventral ganglion relying on Fasciclin2-immunoreactive tracts as three-dimensional landmarks. With tyrosine hydroxylase- (TH) or tyrosine decarboxylase 2 (TDC2)-specific gal4-drivers, we also analyzed the distribution of ectopically expressed neuronal compartment markers in presumptive dopaminergic TH and tyraminergic/octopaminergic TDC2 neurons, respectively. Our results suggest that thoracic and abdominal 5-HT and TH neurons are exclusively interneurons whereas most TDC2 neurons are efferent. 5-HT and TH neurons are ideally positioned to integrate sensory information and to modulate neuronal transmission within the ventral ganglion, while most TDC2 neurons appear to act peripherally. In contrast to 5-HT neurons, TH and TDC2 neurons each comprise morphologically different neuron subsets with separated in- and output compartments in specific neuropil regions. The three-dimensional mapping of aminergic neurons now facilitates the identification of neuronal network contacts and co-localized signaling molecules, as exemplified for DOPA decarboxylase-synthesizing neurons that co-express crustacean cardioactive peptide and myoinhibiting peptides
Unravelling the evolution of the Allatostatin-Type A, KISS and Galanin Peptide-Receptor gene families in Bilaterians: insights from Anopheles Mosquitoes
Allatostatin type A receptors (AST-ARs) are a group of G-protein coupled receptors activated by members of the FGL-amide (AST-A) peptide family that inhibit food intake and development in arthropods. Despite their physiological importance the evolution of the AST-A system is poorly described and relatively few receptors have been isolated and functionally characterised in insects. The present study provides a comprehensive analysis of the origin and comparative evolution of the AST-A system. To determine how evolution and feeding modified the function of AST-AR the duplicate receptors in Anopheles mosquitoes, were characterised. Phylogeny and gene synteny suggested that invertebrate AST-A receptors and peptide genes shared a common evolutionary origin with KISS/GAL receptors and ligands. AST-ARs and KISSR emerged from a common gene ancestor after the divergence of GALRs in the bilaterian genome. In arthropods, the AST-A system evolved through lineage-specific events and the maintenance of two receptors in the flies and mosquitoes (Diptera) was the result of a gene duplication event. Speciation of Anophelesmosquitoes affected receptor gene organisation and characterisation of AST-AR duplicates (GPRALS1 and 2) revealed that in common with other insects, the mosquito receptors were activated by insect AST-A peptides and the iCa(2+)-signalling pathway was stimulated. GPRALS1 and 2 were expressed mainly in mosquito midgut and ovaries and transcript abundance of both receptors was modified by feeding. A blood meal strongly up-regulated expression of both GPRALS in the midgut (p < 0.05) compared to glucose fed females. Based on the results we hypothesise that the AST-A system in insects shared a common origin with the vertebrate KISS system and may also share a common function as an integrator of metabolism and reproduction. Highlights: AST-A and KISS/GAL receptors and ligands shared common ancestry prior to the protostome-deuterostome divergence. Phylogeny and gene synteny revealed that AST-AR and KISSR emerged after GALR gene divergence. AST-AR genes were present in the hemichordates but were lost from the chordates. In protostomes, AST-ARs persisted and evolved through lineage-specific events and duplicated in the arthropod radiation. Diptera acquired and maintained functionally divergent duplicate AST-AR genes.Foundation for Science and Technology, Portugal (FCT) [PTDC/BIA-BCM/114395/2009]; European Regional Development Fund (ERDF) COMPETE - Operational Competitiveness Programme; Portuguese funds through FCT Foundation for Science and Technology [PEst-C/MAR/LA0015/2013, UID/Multi/04326/2013, PEst-OE/SAU/LA0018/2013]; FCT [SFRH/BPD/89811/2012, SFRH/BPD/80447/2011, SFRH/BPD/66742/2009]; auxiliary research contract FCT Pluriannual funds [PEst-C/MAR/LA0015/2013, UID/Multi/04326/2013]info:eu-repo/semantics/publishedVersio
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