A novel method for spectrophotometric determination of pregabalin in pure form and in capsules

<p>Abstract</p> <p>Background</p> <p>Pregabalin, a γ-amino-n-butyric acid derivative, is an antiepileptic drug not yet official in any pharmacopeia and development of analytical procedures for this drug in bulk/formulation forms is a necessity. We herein, report a new, simple, extraction free, cost effective, sensitive and reproducible spectrophotometric method for the determination of the pregabalin.</p> <p>Results</p> <p>Pregabalin, as a primary amine was reacted with ninhydrin in phosphate buffer pH 7.4 to form blue violet colored chromogen which could be measured spectrophotometrically at λ_max402.6 nm. The method was validated with respect to linearity, accuracy, precision and robustness. The method showed linearity in a wide concentration range of 50-1000 μg mL^-1with good correlation coefficient (0.992). The limits of assays detection was found to be 6.0 μg mL^-1and quantitation limit was 20.0 μg mL^-1. The suggested method was applied to the determination of the drug in capsules. No interference could be observed from the additives in the capsules. The percentage recovery was found to be 100.43 ± 1.24.</p> <p>Conclusion</p> <p>The developed method was successfully validated and applied to the determination of pregabalin in bulk and pharmaceutical formulations without any interference from common excipients. Hence, this method can be potentially useful for routine laboratory analysis of pregabalin.</p

EFFECT OF TETRA-ATAXIOMETRIC POSTUROGRAPHY PROGRAMS (TETRAX) ON STROKE PATIENTS WITH IMPAIRED STANDING BALANCE

Patients are suffering from stroke experience balance dysfunction leading to poor weight shifts and reduced weight bearing on the affected lower limb. The Tetrax biofeedback system is a Centre of Pressure-controlled, video game-based exercise system designed for patients with balance dysfunction. Objective: This study aimed to investigate the effects of using Tetrax balance games and conventional rehabilitation on the balance and mobility of subjects with impaired standing balance due to stroke. Design: Pretest-posttest Randomized Clinical Trial Method: A total of thirty patients (including male and female) who have suffered a stroke at least six months back, diagnosed with hemiplegia within the age group of 35-65 years with MMSE Score = 24 were recruited. Six subjects did not give their consent. Subjects were assigned to Group 1 (test group) and Group 2 (control group), using Convenience sampling. Group 1 (test group) consisted of 12 subjects (both male and female) and received conventional therapy and tetrax sessions. Group 2 (control group) consisted of 12 subjects (both male and female) and received only conventional therapy. All participants received conventional outpatient rehabilitation therapy (which accounted for an average of 60 minutes of physiotherapy for the control group and 40 minutes for the test group per day) three days a week in an outpatient setting. The test group received additional Tetrax biofeedback balance training three days per week (20 minutes per day) for four weeks in the same outpatient setting. The Tetrax system was equipped with four independent force plates under the toes and heels. The outcome measures of the study were BBs and FIMS. All outcome measures were evaluated pretest and posttest. Result: The means standard deviations of Improvement in BBS in test and control groups are 3.83 4.64 and 1.25 1.28, respectively. On applying the t-Test, we found the mean difference of 0.077 of Improvement in BBS in the test and control group is not significant. The means standard deviations of Improvement in FIMS in test and control groups are 2.83 2.24 and 1.75 1.21, respectively. On applying the t-Test, we found the mean difference of 0.156 of Improvement in FIMS in the test and control group is not significant. Conclusion: The current study found that both groups those receiving only conventional therapy and those receiving a combination of tetrax and conventional therapy improved their balance significantly, although there was no significant difference between them

Acquisition and Homeostasis of Iron in Higher Plants and Their Probable Role in Abiotic Stress Tolerance

Iron (Fe) is a micronutrient that plays an important role in agriculture worldwide because plants require a small amount of iron for its growth and development. All major functions in a plant's life from chlorophyll biosynthesis to energy transfer are performed by Fe (Brumbarova et al., 2008; Gill and Tuteja, 2011). Iron also acts as a major constituent of many plant proteins and enzymes. The acquisition of Fe in plants occurs through two strategies, i.e., strategy I and strategy II (Marschner and Römheld, 1994). Under various stress conditions, Nramp and the YSL gene families help in translocation of Fe, which further acts as a mineral regulatory element and defends plants against stresses. Iron plays an irreplaceable role in alleviating stress imposed by salinity, drought, and heavy metal stress. This is because it activates plant enzymatic antioxidants like catalase (CAT), peroxidase, and an isoform of superoxide dismutase (SOD) that act as a scavenger of reactive oxygen species (ROS) (Hellin et al., 1995). In addition to this, their deficiency as well as their excess amount can disturb the homeostasis of a plant's cell and result in declining of photosynthetic rate, respiration, and increased accumulation of Na+ and Ca− ions which culminate in an excessive formation of ROS. The short-range order hydrated Fe oxides and organic functional groups show affinities for metal ions. Iron plaque biofilm matrices could sequester a large amount of metals at the soil–root interface. Hence, it has attracted the attention of plant physiologists and agricultural scientists who are discovering more exciting and hidden applications of Fe and its potential in the development of bio-factories. This review looks into the recent progress made in putting forward the role of Fe in plant growth, development, and acclimation under major abiotic stresses, i.e., salinity, drought, and heavy metals

Proceedings of International Conference on Women Researchers in Electronics and Computing

This proceeding contains articles on the various research ideas of the academic community and practitioners presented at the international conference, “Women Researchers in Electronics and Computing” (WREC’2021). WREC'21 was organized in online mode by Dr. B R Ambedkar National Institute of Technology, Jalandhar (Punjab), INDIA during 22 – 24 April 2021. This conference was conceptualized with an objective to encourage and motivate women engineers and scientists to excel in science and technology and to be the role models for young girls to follow in their footsteps. With a view to inspire women engineers, pioneer and successful women achievers in the domains of VLSI design, wireless sensor networks, communication, image/ signal processing, machine learning, and emerging technologies were identified from across the globe and invited to present their work and address the participants in this women oriented conference. Conference Title: International Conference on Women Researchers in Electronics and ComputingConference Acronym: WREC'21Conference Date: 22–24 April 2021Conference Location: Online (Virtual Mode)Conference Organizers: Department of Electronics and Communication Engineering, Dr. B. R. Ambedkar National Institute of Technology, Jalandhar, Punjab, INDI