Frequency-dependent mobilization of heterogeneous pools of synaptic vesicles shapes presynaptic plasticity

The segregation of the readily releasable pool of synaptic vesicles (RRP) in sub-pools that are differentially poised for exocytosis shapes short-term plasticity. However, the frequency-dependent mobilization of these sub-pools is poorly understood. Using slice recordings and modeling of synaptic activity at cerebellar granule cell to Purkinje cell synapses of mice, we describe two sub-pools in the RRP that can be differentially recruited upon ultrafast changes in the stimulation frequency. We show that at low frequency stimulations, a first sub-pool is gradually silenced, leading to full blockage of synaptic transmission. Conversely, a second pool of synaptic vesicles that cannot be released by a single stimulus is recruited within milliseconds by high-frequency stimulation and support an ultrafast recovery of neurotransmitter release after low-frequency depression. This frequency-dependent mobilization or silencing of sub-pools in the RRP in terminals of granule cells may play a role in the filtering of sensorimotor information in the cerebellum

The Stellar Population Histories of Early-Type Galaxies. II. Controlling Parameters of the Stellar Populations

We analyze single-stellar-population (SSP) equivalent parameters for 50 local elliptical galaxies as a function of their structural parameters. These galaxies fill a two-dimensional plane in the four-dimensional space of [Z/H], log t, log $\sigma$, and [E/Fe]. SSP age and velocity dispersion can be taken as the two independent parameters that specify a galaxy's location in this ``hyperplane.'' The hyperplane can be decomposed into two sub-relations: (1) a ``Z-plane,'' in which [Z/H] is a linear function of log $\sigma$ and log t; and (2) a relation between [E/Fe] and $\sigma$ in which [E/Fe] is larger in high-$\sigma$ galaxies. Cluster and field ellipticals follow the same hyperplane, but their ($\sigma$,t) distributions within it differ. Nearly all cluster galaxies are old; the field ellipticals span a large range in SSP age. The tight Mg--$\sigma$ relations of these ellipticals can be understood as two-dimensional projections of the metallicity hyperplane showing it edge-on; the tightness of these relations does not necessarily imply a narrow range of ages at fixed $\sigma$. The relation between [E/Fe] and $\sigma$ is consistent with a higher effective yield of Type II SNe elements at higher $\sigma$. The Z-plane is harder to explain and may be a powerful clue to star formation in elliptical galaxies if it proves to be general. Present data favor a ``frosting'' model in which low apparent SSP ages are produced by adding a small frosting of younger stars to an older base population. If the frosting abundances are close to or slightly greater than the base population, simple two-component models run along lines of constant $\sigma$ in the Z-plane, as required. This favors star formation from well-mixed pre-enriched gas rather than unmixed low-metallicity gas from an accreted object. (Abridged)Comment: To be published in the June 2000 issue of the Astronomical Journal. 28 pages, 13 figures, uses emulateap

Climate reconstruction based on archaeological bivalve shells

Several years of biogeochemical research on bivalve shells yielded in clear proxyrecords carrying potential for reconstruction of paleoseasonal trends in coastal environments. However, the interpretation of the proxy signals is still often problematic. Proxy concentrations can be influenced by several environmental parameters and by physiological processes. With more complex models these problems can be tackled. Two strategies are followed; (1) a statistical black-box model is being developed in parallel with (2) a physiological white-box model.The statistical black-box model can be described as a non-linear multi-proxy model. It is based on chemical measurements in modern bivalve shells and consists of the construction of a curve in a multi-dimensional space. The model describes the variations in the chemical signature of the shell during a full year cycle. The shortest distance from any other data point (e.g. a fossil shell) to the model will give a time point estimation in the annual cycle, which can further be linked to environmental parameters. At present our model approach achieves quite accurate SST reconstructions.A white box model is crucial for understanding the physiological processes and for an unambiguous interpretation of the proxy records. We investigated, in a first phase, in situ the influences of environmental parameters and physiology on the incorporation of proxies in Mytilus edulis at a well documented wave breaker site. In a second phase, in vitro culturing experiments under controlled laboratory conditions were carried out. Experiments were carried out at 8°C and 16°C and at salinities of 18‰ and 28‰. During these experiments mussels were fed under high and low supply regimes. By combining these in situ and in vitro approaches a white box multi-proxy model is generated for the reconstruction of SST and SSS

Optically variable active galactic nuclei in the 3 yr VST survey of the COSMOS field

The analysis of the variability of active galactic nuclei (AGNs) at different wavelengths and the study of possible correlations among different spectral windows are nowadays a major field of inquiry. Optical variability has been largely used to identify AGNs in multivisit surveys. The strength of a selection based on optical variability lies in the chance to analyze data from surveys of large sky areas by ground-based telescopes. However the effectiveness of optical variability selection, with respect to other multiwavelength techniques, has been poorly studied down to the depth expected from next generation surveys. Here we present the results of our r-band analysis of a sample of 299 optically variable AGN candidates in the VST survey of the COSMOS field, counting 54 visits spread over three observing seasons spanning > 3 yr. This dataset is > 3 times larger in size than the one presented in our previous analysis (De Cicco et al. 2015), and the observing baseline is ~8 times longer. We push towards deeper magnitudes (r(AB) ~23.5 mag) compared to past studies; we make wide use of ancillary multiwavelength catalogs in order to confirm the nature of our AGN candidates, and constrain the accuracy of the method based on spectroscopic and photometric diagnostics. We also perform tests aimed at assessing the relevance of dense sampling in view of future wide-field surveys. We demonstrate that the method allows the selection of high-purity (> 86%) samples. We take advantage of the longer observing baseline to achieve great improvement in the completeness of our sample with respect to X-ray and spectroscopically confirmed samples of AGNs (59%, vs. ~15% in our previous work), as well as in the completeness of unobscured and obscured AGNs. The effectiveness of the method confirms the importance to develop future, more refined techniques for the automated analysis of larger datasets.Comment: 21 pages, 10 figures; accepted for publication in A&

Aortic valve replacement with the minimal extracorporeal circulation (Jostra MECC System) versus standard cardiopulmonary bypass: A randomized prospective trial

AbstractBackgroundWe prospectively evaluated a newly introduced minimal extracorporeal circulation system (Jostra MECC System; Jostra AG, Hirrlingen, Germany) for aortic valve surgery.MethodIn a prospective, randomized study, 100 patients underwent aortic valve replacement either with standard cardiopulmonary bypass (n = 50, group B) or with the MECC System (n = 50, group B). The myocardial protection and the left vent were identical for the two groups. The intrapericardial suction device was never used (only the cell salvage device was used) to reduce the air-blood contact area.ResultsNo significant differences were noted in patient characteristics and operative data between groups. Operative mortality (<30 days) was 2% for group A and 4% for group B (difference not significant). From the preoperative period to the postoperative period, the increase in C-reactive protein was significantly higher for group B (P < .001). The postoperative troponin I level was significantly lower in group A (mean 4.65 ± 2.9 μg/L at 24 hours) than in group B (8.2 ± 4.4 μg/L, P < .03). On the other hand, the MECC System was associated with platelet preservation. Renal function was better preserved and the neurologic event rate was significantly lower for the MECC group (P < .02).ConclusionThe MECC System is safe and allows aortic valve replacement under the most favorable conditions. The system is more biocompatible than standard cardiopulmonary bypass and provides a good postoperative biologic profile and good clinical results, particularly for high-risk patients

The Age Difference between the Globular Cluster Sub-populations in NGC 4472

The age difference between the two main globular cluster sub-populations in the Virgo giant elliptical galaxy, NGC 4472 (M 49), has been determined using HST WFPC2 images in the F555W and F814W filters. Accurate photometry has been obtained for several hundred globular clusters in each of the two main sub-populations, down to more than one magnitude below the turn-over of their luminosity functions. This allows precise determinations of both the mean colors and the turn-over magnitudes of the two main sub-populations. By comparing the data with various population synthesis models, the age-metallicity pairs that fit both the observed colors and magnitudes have been identified. The metal-poor and the metal-rich globular clusters are found to be coeval within the errors ($\sim 3$ Gyr). If one accepts the validity of our assumptions, these errors are dominated by model uncertainties. A systematic error of up to 4 Gyr could affect this result if the blue and the red clusters have significantly different mass distributions. However, that one sub-population is half as old as the other is excluded at the 99% confidence level. The different globular cluster populations are assumed to trace the galaxy's major star-formation episodes. Consequently, the vast majority of globular clusters, and by implication the majority of stars, in NGC 4472 formed at high redshifts but by two distinct mechanisms or in two episodes.Comment: 32 pages, including 12 postscript figures, accepted for publication in the Astronomical Journal, December 1999 issu

Estimation of the in vivo recombination rate for a plant RNA virus

[EN] Phylogenomic evidence suggested that recombination is an important evolutionary force for potyviruses, one of the larger families of plant RNA viruses. However, mixed-genotype potyvirus infections are marked by low levels of cellular coinfection, precluding template switching and recombination events between virus genotypes during genomic RNA replication. To reconcile these conflicting observations, we evaluated the in vivo recombination rate (r(g)) of Tobacco etch virus (TEV; genus Potyvirus, family Potyviridae) by coinfecting plants with pairs of genotypes marked with engineered restriction sites as neutral markers. The recombination rate was then estimated using two different approaches: (i) a classical approach that assumed recombination between marked genotypes can occur in the whole virus population, rendering an estimate of r(g)=7.762x10(-8) recombination events per nucleotide site per generation, and (ii) an alternative method that assumed recombination between marked genotypes can occur only in coinfected cells, rendering a much higher estimate of r(g)=3.427x10(-8) recombination events per nucleotide site per generation. This last estimate is similar to the TEV mutation rate, suggesting that recombination should be at least as important as point mutation in creating variability. Finally, we compared our mutation and recombination rate estimates to those reported for animal RNA viruses. Our analysis suggested that high recombination rates may be an unavoidable consequence of selection for fast replication at the cost of low fidelity.We thank Francisca de la Iglesia and Angels Prosper for excellent technical assistance, Jose A. Dare's for methodological advice, Jose M. Cuevas for critical reading of the manuscript, and other lab members for helpful discussions. This work was supported by the Spanish Secretaria de Estado de Investigacion, Desarrollo e Innovacion (grants BFU2009-06993 and BFU2012-30805). N. T. was supported,by a pre-doctoral fellowship from the former Spanish Ministerio de Ciencia e Innovacion.Tromas, N.; Zwart, MP.; Poulain, M.; Elena Fito, SF. (2014). Estimation of the in vivo recombination rate for a plant RNA virus. Journal of General Virology. 95:724-732. https://doi.org/10.1099/vir.0.060822-0S7247329

Comparison of Gene Repertoires and Patterns of Evolutionary Rates in Eight Aphid Species That Differ by Reproductive Mode

In theory, the loss of sexual reproduction is expected to result in the accumulation of deleterious mutations. In aphids, two main types of life cycle, cyclic and obligate parthenogenesis, represent respectively “sexual” and “asexual” reproductive modes. We used the complete pea aphid genome and previously published expressed sequence tags (ESTs) from two other aphid species. In addition, we obtained 100,000 new ESTs from five more species. The final set comprised four sexual and four asexual aphid species and served to test the influence of the reproductive mode on the evolutionary rates of genes. We reconstructed coding sequences from ESTs and annotated these genes, discovering a novel peptide gene family that appears to be among the most highly expressed transcripts from several aphid species. From 203 genes found to be 1:1 orthologs among the eight species considered, we established a species tree that partly conflicted with taxonomy (for Myzus ascalonicus). We then used this topology to evaluate the dynamics of evolutionary rates and mutation accumulation in the four sexual and four asexual taxa. No significant increase of the nonsynonymous to synonymous ratio or of nonsynonymous mutation numbers was found in any of the four branches for asexual taxa. We however found a significant increase of the synonymous rate in the branch leading to the asexual species Rhopalosiphum maidis, which could be due to a change in the mutation rate or to an increased number of generations implied by its change of life cycle

Towards closing the gap between hygroscopic growth and CCN activation for secondary organic aerosols-Part 3: Influence of the chemical composition on the hygroscopic properties and volatile fractions of aerosols

The influence of varying levels of water mixing ratio,r during the formation of secondary organic aerosol (SOA) from the ozonolysis of α-pinene on the SOA hygroscopicity and volatility was investigated. The reaction proceeded and aerosols were generated in a mixing chamber and the hygroscopic characteristics of the SOA were determined with the Leipzig Aerosol Cloud Interaction Simulator (LACIS) and a Cloud Condensation Nuclei counter (CCNc). In parallel, a High-Resolution Time-of-Flight Aerodyne Aerosol Mass Spectrometer (HR-ToF-AMS) located downstream of a thermodenuder (TD) sampling from the mixing chamber, to collect mass spectra of particles from the volatile and less-volatile fractions of the SOA. Results showed that both hygroscopic growth and the volatile fraction of the SOA increased with increases in r inside the mixing chamber during SOA generation. An effective density of 1.40 g cm-3 was observed for the generated SOA when the reaction proceeded with <1 g kg-1. Changes in the concentrations of the fragment CO2+ and the sum of CxH+y(short name CHO) and CxH+y (short name CH) fragments as measured by the HR-ToF-AMS were used to estimate changes in the oxidation level of the SOA with reaction conditions, using the ratios CO2 + to CH and CHO to CH. Under humid conditions, both ratios increased, corresponding to the presence of more oxygenated functional groups (i.e., multifunctional carboxylic acids). This result is consistent with the α-pinene ozonolysis mechanisms which suggest that water interacts with the stabilized Criegee intermediate. The volatility and the hygroscopicity results show that SOA generation via ozonolysis of &alpha;-pinene in the presence of water vapour (r <16.9 g kg-1) leads to the formation of more highly oxygenated compounds that are more hygroscopic and more volatile than compounds formed under dry conditions. © 2010 Author(s)