Predicting local and non-local effects of resources on animal space use using a mechanistic step selection model

1. Predicting space use patterns of animals from their interactions with the environment is fundamental for understanding the effect of habitat changes on ecosystem functioning. Recent attempts to address this problem have sought to unify resource selection analysis, where animal space use is derived from available habitat quality, and mechanistic movement models, where detailed movement processes of an animal are used to predict its emergent utilization distribution. Such models bias the animal's movement towards patches that are easily available and resource-rich, and the result is a predicted probability density at a given position being a function of the habitat quality at that position. However, in reality, the probability that an animal will use a patch of the terrain tends to be a function of the resource quality in both that patch and the surrounding habitat. 2. We propose a mechanistic model where this non-local effect of resources naturally emerges from the local movement processes, by taking into account the relative utility of both the habitat where the animal currently resides and that of where it is moving. We give statistical techniques to parametrize the model from location data and demonstrate application of these techniques to GPS data of caribou in Newfoundland. 3. Steady-state animal probability distributions arising from the model have complex patterns that cannot be expressed simply as a function of the local quality of the habitat. In particular, large areas of good habitat are used more intensively than smaller patches of equal quality habitat, whereas isolated patches are used less frequently. 4. Whilst we focus on habitats in this study, our modelling framework can be readily used with any environmental covariates and therefore represents a unification of mechanistic modelling and step selection approaches to understanding animal space use

Ecological effects of experimental drought and prescribed fire in a southern California coastal grassland

How drought and fire disturbance influence different levels of biological organization is poorly understood but essential for robust predictions of the effects of environmental change. During a year of severe drought, we conducted a prescribed fire in a Mediterranean-type coastal grassland near Irvine, California. In the weeks following the fire we experimentally manipulated rainfall in burned and unburned portions of the grassland to determine how fire and drought interact to influence leaf physiological performance, community composition, aboveground net primary productivity (ANPP) and component fluxes of ecosystem CO2 exchange and evapotranspiration (ET). Fire increased leaf photosynthesis (A net) and transpiration (T) of the native perennial bunchgrass, Nassella pulchra and the non-native annual grass, Bromus diandrus but did not influence ANPP or net ecosystem CO2 exchange (NEE). Surprisingly, drought only weakly influenced A net and T of both species but strongly influenced ANPP and NEE. We conclude that despite increasing experimental drought severity, prescribed fire influenced leaf CO2 and H2O exchange but had little effect on the component fluxes of ecosystem CO2 exchange. The differential effects of prescribed fire on leaf and ecosystem processes with increasingly severe drought highlight the challenge of predicting the responses of biological systems to disturbance and resource limitation

Altered TMPRSS2 usage by SARS-CoV-2 Omicron impacts infectivity and fusogenicity

The SARS-CoV-2 Omicron BA.1 variant emerged in 20211 and has multiple mutations in its spike protein2. Here we show that the spike protein of Omicron has a higher affinity for ACE2 compared with Delta, and a marked change in its antigenicity increases Omicron’s evasion of therapeutic monoclonal and vaccine-elicited polyclonal neutralizing antibodies after two doses. mRNA vaccination as a third vaccine dose rescues and broadens neutralization. Importantly, the antiviral drugs remdesivir and molnupiravir retain efficacy against Omicron BA.1. Replication was similar for Omicron and Delta virus isolates in human nasal epithelial cultures. However, in lung cells and gut cells, Omicron demonstrated lower replication. Omicron spike protein was less efficiently cleaved compared with Delta. The differences in replication were mapped to the entry efficiency of the virus on the basis of spike-pseudotyped virus assays. The defect in entry of Omicron pseudotyped virus to specific cell types effectively correlated with higher cellular RNA expression of TMPRSS2, and deletion of TMPRSS2 affected Delta entry to a greater extent than Omicron. Furthermore, drug inhibitors targeting specific entry pathways3 demonstrated that the Omicron spike inefficiently uses the cellular protease TMPRSS2, which promotes cell entry through plasma membrane fusion, with greater dependency on cell entry through the endocytic pathway. Consistent with suboptimal S1/S2 cleavage and inability to use TMPRSS2, syncytium formation by the Omicron spike was substantially impaired compared with the Delta spike. The less efficient spike cleavage of Omicron at S1/S2 is associated with a shift in cellular tropism away from TMPRSS2-expressing cells, with implications for altered pathogenesis

Post-acute COVID-19 neuropsychiatric symptoms are not associated with ongoing nervous system injury

A proportion of patients infected with severe acute respiratory syndrome coronavirus 2 experience a range of neuropsychiatric symptoms months after infection, including cognitive deficits, depression and anxiety. The mechanisms underpinning such symptoms remain elusive. Recent research has demonstrated that nervous system injury can occur during COVID-19. Whether ongoing neural injury in the months after COVID-19 accounts for the ongoing or emergent neuropsychiatric symptoms is unclear. Within a large prospective cohort study of adult survivors who were hospitalized for severe acute respiratory syndrome coronavirus 2 infection, we analysed plasma markers of nervous system injury and astrocytic activation, measured 6 months post-infection: neurofilament light, glial fibrillary acidic protein and total tau protein. We assessed whether these markers were associated with the severity of the acute COVID-19 illness and with post-acute neuropsychiatric symptoms (as measured by the Patient Health Questionnaire for depression, the General Anxiety Disorder assessment for anxiety, the Montreal Cognitive Assessment for objective cognitive deficit and the cognitive items of the Patient Symptom Questionnaire for subjective cognitive deficit) at 6 months and 1 year post-hospital discharge from COVID-19. No robust associations were found between markers of nervous system injury and severity of acute COVID-19 (except for an association of small effect size between duration of admission and neurofilament light) nor with post-acute neuropsychiatric symptoms. These results suggest that ongoing neuropsychiatric symptoms are not due to ongoing neural injury

Ecological effects of experimental drought and prescribed fire in a southern California coastal grassland

Howdroughtandfiredisturbance influence different levelsofbiological organization is poorly understood but essential for robust predictionsoftheeffectsofenvironmental change. Duringayearofseveredrought, we conductedaprescribedfireinaMediterranean-typecoastalgrasslandnear Irvine,California.Inthe weeks following thefirewe experimentally manipulated rainfallinburned and unburned portionsofthegrasslandto determine howfireanddroughtinteract to influence leaf physiological performance, community composition, aboveground net primary productivity (ANPP) and component fluxesofecosystem CO2 exchange and evapotranspiration (ET).Fireincreased leaf photosynthesis (A(net)) and transpiration (T)ofthe native perennial bunchgrass, Nassella pulchra and the non-native annual grass, Bromus diandrus but did not influence ANPP or net ecosystem CO2 exchange (NEE). Surprisingly,droughtonly weakly influencedA(net) and Tofboth species but strongly influenced ANPP and NEE. We conclude that despite increasingexperimentaldroughtseverity,prescribedfireinfluenced leaf CO2 and H2O exchange but had little effect on the component fluxesofecosystem CO2 exchange. The differentialeffectsofprescribedfireon leaf and ecosystem processes with increasingly severedroughthighlight the challengeofpredicting the responsesofbiological systems to disturbance and resource limitation

Acetamiprid fate in a sandy loam with contrasting soil organic matter contents: A comparison of the degradation, sorption and leaching of commercial neonicotinoid formulations

The impacts of neonicotinoids have generally focussed on the responses of the pure active ingredient. Using a selection of two commercial formulations and the active ingredient, we ran three laboratory studies using 14C-labelled acetamiprid to study the leaching, sorption and mineralisation behaviours of the commercially available neonicotinoid formulations compared to the pure active ingredient. We added 14C-spiked acetamiprid to a sandy loam soil that had received long-term additions of farmyard manure at two rates (10 t/ha/yr and 25 t/ha/yr) and mineral fertilisers, as a control. We found significant differences in acetamiprid mineralisation across both the SOM and chemical treatments. Sorption was primarily impacted by changes in SOM and any differences in leachate recovery were much less significant across both treatment types. The mineralisation of all pesticide formulations was comparatively slow, with <23 % of any given chemical/soil organic matter combination being mineralised over the experimental period. The highest mineralisation rates occurred in samples with the highest soil organic matter levels. The results also showed that 82.9 % ± 1.6 % of the acetamiprid applied was leached from the soil during repeated simulated rainfall events. This combined with the low sorption values, and the low rates of mineralisation, implies that acetamiprid is highly persistent and mobile within sandy soils. As a highly persistent neurotoxin with high invertebrate selectivity, the presence of neonicotinoids in soil presents a high toxicology risk to various beneficial soil organisms, including earthworms, as well as being at high risk of transfer to surrounding watercourses

Da menina meiga à heroína superpoderosa: infância, gênero e poder nas cenas da ficção e da vida

Este artigo pretende discutir o desenho animado As meninas superpoderosas como um texto midiático que traz à tona a imagem da criança como herói. Analisamos os modos como crianças de uma turma de educação infantil interpretam episódios do desenho animado e, a partir dessas referências simbólicas, produzem sentidos sobre ser criança na contemporaneidade. Com base no diálogo entre os discursos das crianças e aqueles que compõem a narrativa da animação, discutimos os novos mapeamentos das fronteiras entre infância e vida adulta e das experiências masculina e feminina. Destacamos, também, os modos como as meninas produzem uma cultura lúdica, atravessada por referências advindas de outros textos midiáticos, nos quais a imagem da menina poderosa aparece associada à sedução. Por fim, refletimos sobre as contradições da infância contemporânea, situadas entre a desconstrução da imagem da criança frágil e inocente e outras formas de controle adultas, circunscritas pela cultura do consumo