In vitro kinetic study of growth and mineralization of osteoblast-like cells (Saos-2) on titanium surface coated with a RGD functionalized bisphosphonate

Osteoconduction and osseointegration are the critical stages for implantation success. Peptides containing RGD (Arg-Gly-Asp) adhesive sequence are known to promote cell adhesion and consequently to favor osseointegration of medical devices. In this study, RGD peptides were coupled to a bisphosphonate used as an anchor system and chemically adsorbed on polished titanium discs. Two different concentrations, 10−10 mol/L (RGD 10−10) and 10−4 mol/L (RGD 10−4) were compared to non coated discs (RGD 0). Adhesion, spreading, and mineralization of osteoblast-like cells (Saos-2) were assessed. Mineralization kinetic was done at 3, 6, 10, 14, and 18 days of culture; the extent of mineral deposits was quantified by image analysis. Histogram repartitions of nuclear area, characterizing cell spreading, showed a shift to higher values in cells cultured on RGD coated titanium disks. Mineralization started at day 3 in the three groups, but had a faster development in the RGD 10−10 group from day 6 to day 18 compared to RGD 0 and RGD 10−4. At day 18, the percentage of mineralized area was significantly higher for RGD 10−10 compared to RGD 0 (p < 0.05). In the present study, this new method was found suitable to anchor RGD containing species on titanium: this favored adhesion and spreading of osteoblast-like cells and mineralization compared to noncoated titanium

Elaboration of modelized surfaces with well defined microtopochemistry-localization of adsorbed proteins

Using microphotolithographic techniques, we have been able to devise silica surfaces bearing two types of microdomains with an average width of 40\u3bcm. One of the microdomain types was silanized in order to make them hydrophobic. Fourier transform-infrared (FTIR) and Auger electron spectroscopies (AES) were used to check the presence of the expected chemical groups on the modified silica surface. Silica and silanized surface morphology was studied using Scanning Force Microscopy (SFM). Wetting properties were investigated. Protein adsorption studies were performed using diluted human decalcified plasma. Attention was focused on the topographic distribution of the adsorbed molecules in order to establish a correlation between the protein behaviour and the specific chemistry of each type of microdomain. Copyright (C) 2000 Elsevier Science B.V

A FTIR and SEM study of PS radiation grafted fluoropolymers: influence of the nature of the ionizing radiation on the film structure

International audienc

Synthesis of biomaterials by swift heavy ion grafting: Preliminary results of haemocompatibility

International audienc

Cyclo-(DfKRG) peptide grafting onto Ti–6Al–4V: physical characterization and interest towards human osteoprogenitor cells adhesion

In the present paper, specific interest has been devoted to the design of new hybrid materials associating Ti–6Al–4V alloy and osteoprogenitor cells through the grafting of two RGD containing peptides displaying a different conformation (linear RGD and cyclo-DfKRG) onto titanium surface. Biomimetic modification was performed by means of a three-step reaction procedure: silanization with APTES, cross-linking with SMP and finally immobilization of peptides thanks to thiol bonding..