Clinical and pathogenic aspects of vibrational disease and comorbid pathologies.

Despite the tendency to decrease in the level of vibrational disease morbidity, this pathology is third in the structure of occupational diseases in Ukraine. According to research design, we have examined 178 vibrational disease patients of I and II degree due to local vibration. They have been divided into 3 groups: first — 66 patients with vibrational disease and chronic obstructive pulmonary disease; second — 60 patients with vibrational disease and arterial hypertension of II degree, and group of comparison – 52 patients with isolated course of vibrational disease. We have used clinical and physiological, ELISA methods to estimate cytokines levels in these patients. We have found that clinical signs of vibrational disease depend on vibrational disease progressing and comorbid pathologies. Overlay of chronic obstructive pulmonary disease to vibra­tional disease significantly worsens disease course, manifested by augmenting of clinical symptoms, neurovascular and trophic violations. Comorbid course of vibrational disease and arterial hypertension leads to respective worsening of cardio-vascular pathology. One of the mechanisms of development and progressing of vibrational disease is imbalance of cytokine chain of immunity — increase of the amount of inflammatory cytokine TNF-α and decrease of anti-inflammatory — IL-10. Overlay of chronic obstructive pulmonary disease enhances this trend. In patients with comorbid course of vibrational disease and arterial hypertension we have observed development of secondary dysregulatory state of immunocompetent system. This is proved by increased levels of inflammatory cytokines (TNF-α by 62.5 % and IL-8 by 46.5 %), and decreased activity of melatonin by 35.0 % in comparison with group of patients with isolated vibrational disease

Інтернет-комунікації у стратегіях цифровізації бізнесу підприємств сфери послуг

Digitalization of business in the sphere of services is proved to create new terms, forms and technologies of services rendering and to strengthen the enterprise competitive position, while undue considering the actual tendencies in strategies of Internet-communications development can bring the business of rendering the services of certain type to end in failure. The acceleration of processes of electronization of relations between suppliers and consumers of services is illustrated. Advantages of digital communications over the traditional ones are stated. These advantages are: freedom from territorial limitations for distribution of communication messages, considerably less charges per contact, possibility of feed-back, possibility of real-time monitoring of effeciency of every communication event, easy access of consumers to information regardless of time and place of the consumers residence.Dividing of facilities of Internet-communications into groups by possibility of applying them by the enterprises of service business is carried out. The actual trends of Internet-communications are analysed. It is shown that strategic prospects of chat-bot usage is predetermined by possibility to provide information and communication in 24/7 mode, by the comfort for a client as well as by the considerable economy of resources. Significance for Internet-communications of such trends as expansion of application of vocal search, complemented and virtual reality, and orientation on mobile devices with geotargetting is emphasized. The importance of business processes automation and of speeding-up the web-site loading is illustrated. The modern Internet user mainly gives preference to viewing the video data instead of re-reading the texts what makes investing in development of creative video-content to be very promising. As potential consumers give more credence to the content created by actual consumers, stimulating the users of services to express their opinions and ideas, to place the photo- and video data as well as systematic elimination of the defects indicated in reviews become important direction of Internet-communications development. The native advertising in the Internet and providing of “transparency” of enterprises-service providers for the real and potential clients gain in significance. It is reasoned that the enterprises of service business must take into account in their marketing strategies the objective trends related to the development of digital technologies as well as to the modern features of exceptionally communicative influence on clients; prerequisites for strengthening of this influence are created by possibilities of the Internet use.Обґрунтовано, що цифровізація бізнесу у сфері послуг створює нові умови, форми і технології їх надання, зміцнює конкурентні позиції підприємств, а несвоєчасне врахування актуальних трендів у стратегіях розвитку Інтернет-комунікацій може призвести до руйнації бізнесу з надання певного виду послуг. Проілюстровано прискорення процесів електронізації стосунків між надавачами і споживачами послуг. Сформульовано переваги цифрових комунікацій над традиційними: відсутність територіальних обмежень для розповсюдження комунікаційних повідомлень, значно менші витрати у розрахунку на один контакт, можливість зворотного зв’язку, можливість відстежувати результативність кожного комунікаційного заходу у режимі реального часу, легкий доступ споживачів до інформації незалежно від часу і місця їх перебування. Здійснено поділ засобів Iнтернет-комунікацій на групи стосовно можливості їх застосування підприємствами сфери послуг. Проаналізовано актуальні тренди Інтернет-комунікацій. Показано, що стратегічна перспективність використання чат-ботів зумовлюється можливістю надання інформації і спілкування у режимі 24/7, зручністю для клієнта, значною економією ресурсів. Підкреслено роль для Інтернет-комунікацій таких трендів, як розширення застосування голосового пошуку, доповненої і віртуальної реальності, орієнтації на мобільні пристрої з гео-таргетингом. Проілюстрована важливість автоматизації бізнес-процесів, підвищення швидкості завантаження сайтів. Сучасний користувач Інтернету переважно віддає перевагу переглядові відеоматеріалу замість перечитування тексту, що зумовлює перспективність інвестування у створення креативного відео-контенту. Оскільки підвищеною довірою потенційних споживачів користується контент, створений фактичними споживачами, важливим напрямом стає стимулювання користувачів послуг щодо висловлення думок, розміщення фото- і відеоматеріалів, а також систематична робота над виправленням недоліків, вказаних у відгуках. Підвищується роль нативної реклами в Інтернеті і забезпечення “прозорості” підприємств-надавачів послуг для своїх реальних і потенційних клієнтів. Обґрунтовано, що підприємства сфери послуг повинні враховувати у своїх маркетингових стратегіях об’єктивні тренди, пов’язані як з розвитком власне цифрових технологій, так і з сучасними особливостями суто комунікативного впливу на клієнтів, поштовх для посилення якого створюють можливості використання Інтернету

Clinical and pathogenic aspects of vibrational disease and comorbid pathologies.

Despite the tendency to decrease in the level of vibrational disease morbidity, this pathology is third in the structure of occupational diseases in Ukraine. According to research design, we have examined 178 vibrational disease patients of I and II degree due to local vibration. They have been divided into 3 groups: first — 66 patients with vibrational disease and chronic obstructive pulmonary disease; second — 60 patients with vibrational disease and arterial hypertension of II degree, and group of comparison – 52 patients with isolated course of vibrational disease. We have used clinical and physiological, ELISA methods to estimate cytokines levels in these patients. We have found that clinical signs of vibrational disease depend on vibrational disease progressing and comorbid pathologies. Overlay of chronic obstructive pulmonary disease to vibra­tional disease significantly worsens disease course, manifested by augmenting of clinical symptoms, neurovascular and trophic violations. Comorbid course of vibrational disease and arterial hypertension leads to respective worsening of cardio-vascular pathology. One of the mechanisms of development and progressing of vibrational disease is imbalance of cytokine chain of immunity — increase of the amount of inflammatory cytokine TNF-α and decrease of anti-inflammatory — IL-10. Overlay of chronic obstructive pulmonary disease enhances this trend. In patients with comorbid course of vibrational disease and arterial hypertension we have observed development of secondary dysregulatory state of immunocompetent system. This is proved by increased levels of inflammatory cytokines (TNF-α by 62.5 % and IL-8 by 46.5 %), and decreased activity of melatonin by 35.0 % in comparison with group of patients with isolated vibrational disease

FINANCIAL ASPECTS OF ESTABLISHING THE INSTITUTION OF THE SELF-EMPLOYMENT

The concept of self-employment as a form of individual entrepreneurship has been considered. A detailed analysis of the experience of foreign countries in establishing the institution of the self-employment,common and distinctive features of the applied models of the development of self-employment in European countries, Australia and the USA, has been presented. Much attention has been paid both the characteristic of the category “self-employed citizens” and the review of their areas of activity in Russia today. Available mechanisms and tools for the development of self-employment have been analyzed. Particular attention has been paid to the analysis of the concept of self-employment and its forms, presented in legislative acts, which serve as the basis for effective regulation by the government. Conclusions and recommendations of the authors on the application of the new special tax treatment on the income of self-employed citizens, implemented as part of a pilot project in several regions of the Russian Federation, have been presented

ВЛИЯНИЕ АБСЦИЗОВОЙ КИСЛОТЫ НА ФОРМИРОВАНИЕ НЕКРОТИЧЕСКОЙ И ХЛОРОЗНОЙ ЗАЩИТНЫХ РЕАКЦИЙ У РАСТЕНИЙ РЖИ (SECALE CEREALE L.) ОТ РЖАВЧИННОЙ ИНФЕКЦИИ

The changes in the ABA contents in rye plants are investigated in the development of different protective reactions against rust infection. A temporary significant increase in the ABA content both in resistant (Secale sp. derzhavinii – Puccinia dispersa), and in incompatible (Secale sereale grade Igumenskaja – Puccinia coronifera) combinations can be a signal to involve antistressful programs in infected tissues directed against pathogen suppression, whereas a high ABA concentration in all pathogenesis stages in a susceptible pathosystem reduces the efficacy of plant antistressful programs and results in the development of disease.В работе исследованы изменения эндогенного содержания АБК в динамике развития хлорозной и некротической защитных реакций в листьях устойчивой и восприимчивой форм ржи. Исходя из полученных данных, можно сделать вывод о том, что временное, значительное увеличение содержания АБК на начальных этапах взаимодействия растения с патогенами как в устойчивой (рожь Державина – Puccinia dispersa), так и в несовместимой (рожь сорта Игуменская – Puccinia coronifera) комбинациях может служить сигналом для включения антистрессовых программ в инфицированных тканях, направленных на подавление активности возбудителя болезни. Тогда как долговременное поддержание высокой концентрации АБК на всех стадиях патогенеза в восприимчивой патосистеме (рожь сорта Игуменская – Puccinia dispersa) приводит к снижению эффективности защитных систем и развитию болезни

Respiratory Syncytial Virus (RSV) RNA loads in peripheral blood correlates with disease severity in mice

<p>Abstract</p> <p>Background</p> <p>Respiratory Syncytial Virus (RSV) infection is usually restricted to the respiratory epithelium. Few studies have documented the presence of RSV in the systemic circulation, however there is no consistent information whether virus detection in the blood correlates with disease severity.</p> <p>Methods</p> <p>Balb/c mice were inoculated with live RSV, heat-inactivated RSV or medium. A subset of RSV-infected mice was treated with anti-RSV antibody 72 h post-inoculation. RSV RNA loads were measured by PCR in peripheral blood from day 1-21 post-inoculation and were correlated with upper and lower respiratory tract viral loads, the systemic cytokine response, lung inflammation and pulmonary function. Immunohistochemical staining was used to define the localization of RSV antigens in the respiratory tract and peripheral blood.</p> <p>Results</p> <p>RSV RNA loads were detected in peripheral blood from day 1 to 14 post-inoculation, peaked on day 5 and significantly correlated with nasal and lung RSV loads, airway obstruction, and blood CCL2 and CXCL1 expression. Treatment with anti-RSV antibody reduced blood RSV RNA loads and improved airway obstruction. Immunostaining identified RSV antigens in alveolar macrophages and peripheral blood monocytes.</p> <p>Conclusions</p> <p>RSV RNA was detected in peripheral blood upon infection with live RSV, followed a time-course parallel to viral loads assessed in the respiratory tract and was significantly correlated with RSV-induced airway disease.</p

Observation of an Excited Bc+ State

Using pp collision data corresponding to an integrated luminosity of 8.5 fb-1 recorded by the LHCb experiment at center-of-mass energies of s=7, 8, and 13 TeV, the observation of an excited Bc+ state in the Bc+π+π- invariant-mass spectrum is reported. The observed peak has a mass of 6841.2±0.6(stat)±0.1(syst)±0.8(Bc+) MeV/c2, where the last uncertainty is due to the limited knowledge of the Bc+ mass. It is consistent with expectations of the Bc∗(2S31)+ state reconstructed without the low-energy photon from the Bc∗(1S31)+→Bc+γ decay following Bc∗(2S31)+→Bc∗(1S31)+π+π-. A second state is seen with a global (local) statistical significance of 2.2σ (3.2σ) and a mass of 6872.1±1.3(stat)±0.1(syst)±0.8(Bc+) MeV/c2, and is consistent with the Bc(2S10)+ state. These mass measurements are the most precise to date

BPAG1a and b Associate with EB1 and EB3 and Modulate Vesicular Transport, Golgi Apparatus Structure, and Cell Migration in C2.7 Myoblasts

BPAG1a and BPAG1b (BPAG1a/b) constitute two major isoforms encoded by the dystonin (Dst) gene and show homology with MACF1a and MACF1b. These proteins are members of the plakin family, giant multi-modular proteins able to connect the intermediate filament, microtubule and microfilament cytoskeletal networks with each other and to distinct cell membrane sites. They also serve as scaffolds for signaling proteins that modulate cytoskeletal dynamics. To gain better insights into the functions of BPAG1a/b, we further characterized their C-terminal region important for their interaction with microtubules and assessed the role of these isoforms in the cytoskeletal organization of C2.7 myoblast cells. Our results show that alternative splicing does not only occur at the 5′ end of Dst and Macf1 pre-mRNAs, as previously reported, but also at their 3′ end, resulting in expression of additional four mRNA variants of BPAG1 and MACF1. These isoform-specific C-tails were able to bundle microtubules and bound to both EB1 and EB3, two microtubule plus end proteins. In the C2.7 cell line, knockdown of BPAG1a/b had no major effect on the organization of the microtubule and microfilament networks, but negatively affected endocytosis and maintenance of the Golgi apparatus structure, which became dispersed. Finally, knockdown of BPAG1a/b caused a specific decrease in the directness of cell migration, but did not impair initial cell adhesion. These data provide novel insights into the complexity of alternative splicing of Dst pre-mRNAs and into the role of BPAG1a/b in vesicular transport, Golgi apparatus structure as well as in migration in C2.7 myoblasts

Observation of the Decay Λ0b→Λ+cτ−¯ν

The first observation of the semileptonic b-baryon decay Λb0→Λc+τ-ν¯τ, with a significance of 6.1σ, is reported using a data sample corresponding to 3 fb-1 of integrated luminosity, collected by the LHCb experiment at center-of-mass energies of 7 and 8 TeV at the LHC. The τ- lepton is reconstructed in the hadronic decay to three charged pions. The ratio K=B(Λb0→Λc+τ-ν¯τ)/B(Λb0→Λc+π-π+π-) is measured to be 2.46±0.27±0.40, where the first uncertainty is statistical and the second systematic. The branching fraction B(Λb0→Λc+τ-ν¯τ)=(1.50±0.16±0.25±0.23)% is obtained, where the third uncertainty is from the external branching fraction of the normalization channel Λb0→Λc+π-π+π-. The ratio of semileptonic branching fractions R(Λc+)B(Λb0→Λc+τ-ν¯τ)/B(Λb0→Λc+μ-ν¯μ) is derived to be 0.242±0.026±0.040±0.059, where the external branching fraction uncertainty from the channel Λb0→Λc+μ-ν¯μ contributes to the last term. This result is in agreement with the standard model prediction

Measurement of antiproton production from antihyperon decays in pHe collisions at √sNN=110GeV

The interpretation of cosmic antiproton flux measurements from space-borne experiments is currently limited by the knowledge of the antiproton production cross-section in collisions between primary cosmic rays and the interstellar medium. Using collisions of protons with an energy of 6.5 TeV incident on helium nuclei at rest in the proximity of the interaction region of the LHCb experiment, the ratio of antiprotons originating from antihyperon decays to prompt production is measured for antiproton momenta between 12 and 110GeV\!/c . The dominant antihyperon contribution, namely Λ¯ → p¯ π+ decays from promptly produced Λ¯ particles, is also exclusively measured. The results complement the measurement of prompt antiproton production obtained from the same data sample. At the energy scale of this measurement, the antihyperon contributions to antiproton production are observed to be significantly larger than predictions of commonly used hadronic production models