Development and use of Сherenkov-type detectors for measurements of fast electrons in tokamaks

The paper reports on progress in design and use of novel detectors for experimental studies of fast (run-away and ripple-born) electrons in various experiments of the tokamak type. The idea of the use of a Cherenkov effect for direct on-line measurements of the fast electrons within tokamaks was presented by scientists from the NCBJ (former IPJ) several years ago. Successive efforts led to the development of prototype detector heads equipped with diamond or aluminium nitrate (AlN) crystals, which were shielded with very thin metal filters in order to eliminate the visible light from plasma and to enable a rough energy analysis of electrons. Those Cherenkov radiators were coupled through optical-fibre cables with fast photomultipliers. Those prototypes were applied for test measurements within the CASTOR experiment in Prague, and later in the ISSTOK device in Lisbon, but the main aim remained to develop the Cherenkov detectors for the TORE-SUPRA experiment in Cadarache.В этой статье представлен прогресс в разработке и использовании новаторских детекторов для экспериментального исследования быстрых (убегающих и генерируемых на неоднородностях) электронов в различных экспериментах на токамаке. Идея использования эффекта Черенкова для прямых измерений быстрых электронов в режиме реального времени в токамаке был представлен учеными из НЦЯП (бывший ИЯП) несколько лет назад. Последовательные усилия привели к разработке прототипа детекторных головок оснащенного кристаллами алмаза или нитрата алюминия (AlN), которые были покрыты очень тонкими металлическими фильтрами для исключения влияния видимого света из плазмы и позволяющими грубо оценить энергию электронов. Эти счетчики Черенкова были соединены через волоконно-оптические кабели с быстрыми ФЭУ. Эти прототипы были применены для тестовых измерений в экспериментах на CASTOR в Праге, а затем в Лиссабоне на установке ISSTOK, но главной целью остается разработка детекторов Черенкова для экспериментов на Tore-Supra в Кадараше.У цій статті йдеться про прогрес у розробці та використанні новаторських детекторів для експериментального дослідження швидких (утеклих і тих., що генеруються на неоднорідностях) електронів в різних експериментах на токамаці. Ідея використання ефекту Черенкова для прямих вимірювань швидких електронів в режимі реального часу в токамаке був представлений вченими з НЦЯП (колишній ІЯП) кілька років тому. Послідовні зусилля привели до розробки прототипу детекторних головок оснащеного кристалами алмазу або нітрату алюмінію (AlN), які були покриті дуже тонкими металевими фільтрами для виключення впливу видимого світла з плазми і дозволяють грубо оцінити енергію електронів. Ці лічильники Черенкова були з'єднані через волоконно-оптичні кабелі з швидкими ФЕУ. Ці прототипи були застосовані для тестових вимірювань в експериментах на CASTOR в Празі, а потім в Лісабоні на установці ISSTOK, але головною метою залишається розробка детекторів Черенкова для експериментів на Tore-Supra в Кадараші

No observed effect of homologous recombination on influenza C virus evolution

The occurrence of homologous recombination in influenza viruses has been under some debate recently. To determine the extent of homologous recombination in influenza C virus, recombination analyses of all available gene sequences of influenza C virus were carried out. No recombination signal was found. With the previous evidence in influenza A and B viruses, it seems that homologous recombination has minimal or no effect on influenza virus evolution

Production and characterization of a recombinant single-chain antibody against Hantaan virus envelop glycoprotein

Hantaan virus (HTNV) is the type of Hantavirus causing hemorrhagic fever with renal syndrome, for which no specific therapeutics are available so far. Cell type-specific internalizing antibodies can be used to deliver therapeutics intracellularly to target cell and thus, have potential application in anti-HTNV infection. To achieve intracellular delivery of therapeutics, it is necessary to obtain antibodies that demonstrate sufficient cell type-specific binding, internalizing, and desired cellular trafficking. Here, we describe the prokaryotic expression, affinity purification, and functional testing of a single-chain Fv antibody fragment (scFv) against HTNV envelop glycoprotein (GP), an HTNV-specific antigen normally located on the membranes of HTNV-infected cells. This HTNV GP-targeting antibody, scFv3G1, was produced in the cytoplasm of Escherichia coli cells as a soluble protein and was purified by immobilized metal affinity chromatography. The purified scFv possessed a high specific antigen-binding activity to HTNV GP and HTNV-infected Vero E6 cells and could be internalized into HTNV-infected cells probably through the clathrin-dependent endocytosis pathways similar to that observed with transferrin. Our results showed that the E. coli-produced scFv had potential applications in targeted and intracellular delivery of therapeutics against HTNV infections

Pathology of Puumala hantavirus infection in macaques

Peer reviewe

Geo-spatial Hotspots of Hemorrhagic Fever with Renal Syndrome and Genetic Characterization of Seoul Variants in Beijing, China

Hemorrhagic fever with renal syndrome (HFRS) is caused by Hantaviruses, the enzootic viruses with a worldwide distribution. In China, HFRS is a significant public health problem with more than 10,000 human cases reported annually and the endemic areas of the disease have extended from rural to urban areas and even to central cities in recent years. The HFRS incidence has increased recently and the morbidity seemed to be considerably diverse in different areas in Beijing, the capital of China. With the aim of gaining more information to control this disease, we carried out a spatial analysis of HFRS based on the data from human cases during 2004–2006 and investigated the genetic features of complete S and partial L segment sequences of Seoul virus from natural infected rodent hosts and patients. We found three geo-spatial clusters, i.e., “hotspots” of HFRS in Beijing, where intervention should be enhanced. Our data indicated that the genetic variation and recombination of SEOV might be related to the high risk areas of HFRS in Beijing, which was worthy of further investigation

Immunological Mechanisms Mediating Hantavirus Persistence in Rodent Reservoirs

Hantaviruses, similar to several emerging zoonotic viruses, persistently infect their natural reservoir hosts, without causing overt signs of disease. Spillover to incidental human hosts results in morbidity and mortality mediated by excessive proinflammatory and cellular immune responses. The mechanisms mediating the persistence of hantaviruses and the absence of clinical symptoms in rodent reservoirs are only starting to be uncovered. Recent studies indicate that during hantavirus infection, proinflammatory and antiviral responses are reduced and regulatory responses are elevated at sites of increased virus replication in rodents. The recent discovery of structural and non-structural proteins that suppress type I interferon responses in humans suggests that immune responses in rodent hosts could be mediated directly by the virus. Alternatively, several host factors, including sex steroids, glucocorticoids, and genetic factors, are reported to alter host susceptibility and may contribute to persistence of hantaviruses in rodents. Humans and reservoir hosts differ in infection outcomes and in immune responses to hantavirus infection; thus, understanding the mechanisms mediating viral persistence and the absence of disease in rodents may provide insight into the prevention and treatment of disease in humans. Consideration of the coevolutionary mechanisms mediating hantaviral persistence and rodent host survival is providing insight into the mechanisms by which zoonotic viruses have remained in the environment for millions of years and continue to be transmitted to humans

Replicating viral vector platform exploits alarmin signals for potent CD8⁺ T cell-mediated tumour immunotherapy.

Viral infections lead to alarmin release and elicit potent cytotoxic effector T lymphocyte (CTL <sup>eff</sup> ) responses. Conversely, the induction of protective tumour-specific CTL <sup>eff</sup> and their recruitment into the tumour remain challenging tasks. Here we show that lymphocytic choriomeningitis virus (LCMV) can be engineered to serve as a replication competent, stably-attenuated immunotherapy vector (artLCMV). artLCMV delivers tumour-associated antigens to dendritic cells for efficient CTL priming. Unlike replication-deficient vectors, artLCMV targets also lymphoid tissue stroma cells expressing the alarmin interleukin-33. By triggering interleukin-33 signals, artLCMV elicits CTL <sup>eff</sup> responses of higher magnitude and functionality than those induced by replication-deficient vectors. Superior anti-tumour efficacy of artLCMV immunotherapy depends on interleukin-33 signalling, and a massive CTL <sup>eff</sup> influx triggers an inflammatory conversion of the tumour microenvironment. Our observations suggest that replicating viral delivery systems can release alarmins for improved anti-tumour efficacy. These mechanistic insights may outweigh safety concerns around replicating viral vectors in cancer immunotherapy

Photochemistry Of Monochloro Complexes Of Copper(ii) In Methanol Probed By Ultrafast Transient Absorption Spectroscopy

Ultrafast transient absorption spectra in the deep to near UV range (212-384 nm) were measured for the [Cu-II(MeOH)(5)Cl](+) complexes in methanol following 255-nm excitation of the complex into the ligand-to-metal charge-transfer excited state. The electronically excited complex undergoes sub-200 fs radiationless decay, predominantly via back electron transfer, to the hot electronic ground state followed by fast vibrational relaxation on a 0.4-4 Ps time scale. A minor photochemical channel is Cu-Cl bond dissociation, leading to the reduction of copper(H) to copper(I) and the formation of MeOH center dot Cl charge-transfer complexes. The depletion of ground-state [Cu-II(MeOH)(5)Cl](+) perturbs the equilibrium between several forms of copper(II) complexes present in solution. Complete re-equilibration between [Cu-II(MeOH)(5)Cl](+) and [Cu-II(MeOH)(4)Cl-2] is established on a 10-500 ps time scale, slower than methanol diffusion, suggesting that the involved ligand exchange mechanism is dissociative

ICRF Wall Conditioning: Present Status and Developments for Future Superconducting Fusion Machines

. 1 ABSTRACT. ITER and future superconducting fusion machines need efficient wall conditioning techniques for routine operation in between shots in the presence of permanent high magnetic field for wall cleaning, surface isotope exchange and to control the in-vessel long term tritium retention. Ion Cyclotron Wall Conditioning (ICWC) based on the ICRF discharge is fully compatible and needs the presence of the magnetic field. The present paper focuses on the principal aspects of the ICWC discharge performance in large-size fusion machines: (i) neutral gas RF breakdown with conventional ICRF heating antennas, (ii) antenna coupling with low density (~10 17 m -3 ) RF plasmas and (iii) ICWC scenarios with improved RF plasma homogeneity in the radial and poloidal directions. All these factors were identified as crucial to achieve an enhanced conditioning effect (e.g. removal rates of selected "marker" masses). All the observed effects are analyzed in terms of RF plasma wave excitation/absorption and compared with the predictions from 1-D RF full wave and 0-D RF plasma codes. Numerical modeling and empirical extrapolation from the existing machines give good evidence for the feasibility of using ICWC in ITER with the main ICRF antenna

Modelling of the effect of ELMs on fuel retention at the bulk W divertor of JET

Effect of ELMs on fuel retention at the bulk W target of JET ITER-Like Wall was studied with multi-scale calculations. Plasma input parameters were taken from ELMy H-mode plasma experiment. The energetic intra-ELM fuel particles get implanted and create near-surface defects up to depths of few tens of nm, which act as the main fuel trapping sites during ELMs. Clustering of implantation-induced vacancies were found to take place. The incoming flux of inter-ELM plasma particles increases the different filling levels of trapped fuel in defects. The temperature increase of the W target during the pulse increases the fuel detrapping rate. The inter-ELM fuel particle flux refills the partially emptied trapping sites and fills new sites. This leads to a competing effect on the retention and release rates of the implanted particles. At high temperatures the main retention appeared in larger vacancy clusters due to increased clustering rate