Fully-digital low-frequency lock-in amplifier for photoluminescence measurements

Lock-in amplifiers, used in several experimental physics applications, are instruments performing quadrature demodulation, which is useful when signals are affected by much noise. Generally, commercially-available lock-in amplifiers are very accurate, but expensive, especially if their operating range includes radiofrequencies. In many applications, high precision is not necessary for the measurements, but it is preferable to have low-cost, low-weight, compactness and a user-friendly graphical unit interface. In this paper, we describe a new fully-digital low-frequency lock-in amplifier developed at ENEA C.R. Frascati Laboratories for photoluminescence experiments based on an innovative low-cost architecture and processing algorithms. The hardware, firmware and software developed for the whole photoluminescence measurement set-up is presented. The present lock-in was first characterized with synthetic electrical sine wave signals and white noise. A dynamic reserve of 43 dB and a noise figure in the range of 25–44 dB were estimated. These results show compatibility with several measurement applications, such as photoluminescence, and the adequacy of the resolutions with respect to the hardware costs. Finally, preliminary results of photoluminescence measurements are presented

Detection of heavy metals in water using graphene oxide quantum dots: an experimental and theoretical study

In this work, we investigate by ab initio calculations and optical experiments the sensitivity of graphene quantum dots in their use as devices to measure the presence, and concentration, of heavy metals in water. We demonstrate that the quenching or enhancement in the optical response (absorption, emission) depends on the metallic ion considered. In particular, two cases of opposite behaviour are considered in detail: Cd2+, where we observe an increase in the emission optical response for increasing concentration, and Pb2+ whose emission spectra, vice versa, are quenched along the concentration rise. The experimental trends reported comply nicely with the different hydration patterns suggested by the models that are also capable of reproducing the minor quenching/ enhancing effects observed in other ions. We envisage that quantum dots of graphene may be routinely used as cheap detectors to measure the degree of poisoning ions in water

Laser pulse effects on plasma-sprayed and bulk tungsten

Tungsten (W) is considered a promising plasma-facing material for protecting the divertor of the ITER (International Thermonuclear Experimental Reactor). The effects on W of transient thermal loads of high energy occurring in a tokamak under operative conditions have been simulated through a single laser pulse delivered by an Nd:YAG laser. Bulk and plasma-sprayed (PS) samples have been submitted to tests and successively examined via SEM (scanning electron microscopy) observations. In both types of materials, the laser pulse induces similar effects: (i) a crater forms in the spot central area; (ii) all around the area, the ejection and the movement of molten metal give rise to a ridge; (iii) in a more external area, the surface shows plates with jagged boundaries and cracks induced by thermal stresses; (iv) the pores present in the original material become preferred ablation sites. However, the affected surface area in PS samples is larger and asymmetric if compared to that of bulk material. Such a difference has been explained by considering how microstructural characteristics influence heat propagation from the irradiated spot, and it was found that grain size and shape play a decisive role

Colorimetric and fluorescent sensing of copper ions in water through o-phenylenediamine-derived carbon dots

Fluorescent nitrogen and sulfur co-doped carbon dots (NSCDs) were synthesized using a simple one-step hydrothermal method starting from o-phenylenediamine (OPD) and ammonium sulfide. The prepared NSCDs presented a selective dual optical response to Cu(II) in water through the arising of an absorption band at 660 nm and simultaneous fluorescence enhancement at 564 nm. The first effect was attributed to formation of cuprammonium complexes through coordination with amino functional groups of NSCDs. Alternatively, fluorescence enhancement can be explained by the oxidation of residual OPD bound to NSCDs. Both absorbance and fluorescence showed a linear increase with an increase of Cu(II) concentration in the range 1-100 mu M, with the lowest detection limit of 100 nM and 1 mu M, respectively. NSCDs were successfully incorporated in a hydrogel agarose matrix for easier handling and application to sensing. The formation of cuprammonium complexes was strongly hampered in an agarose matrix while oxidation of OPD was still effective. As a result, color variations could be perceived both under white light and UV light for concentrations as low as 10 mu M. Since these color changes were similarly perceived in tap and lake water samples, the present method could be a promising candidate for simple, cost-effective visual monitoring of copper onsite

Colorimetric detection of chromium(VI) ions in water using unfolded-fullerene carbon nanoparticles

Water pollution caused by hexavalent chromium (Cr(VI)) ions represents a serious hazard for human health due to the high systemic toxicity and carcinogenic nature of this metal species. The optical sensing of Cr(VI) through specifically engineered nanomaterials has recently emerged as a versatile strategy for the application to easy-to-use and cheap monitoring devices. In this study, a one-pot oxidative method was developed for the cage opening of C60 fullerene and the synthesis of stable suspensions of N-doped carbon dots in water–THF solutions (N-CDs-W-THF). The N-CDs-W-THF selectively showed variations of optical absorbance in the presence of Cr(VI) ions in water through the arising of a distinct absorption band peaking at 550 nm, i.e., in the transparency region of pristine material. Absorbance increased linearly, with the ion concentration in the range 1–100 µM, thus enabling visual and ratiometric determination with a limit of detection (LOD) of 300 nM. Selectivity and possible interference effects were tested over the 11 other most common heavy metal ions. The sensing process occurred without the need for any other reactant or treatment at neutral pH and within 1 min after the addition of chromium ions, both in deionized and in real water sam-ples

Phospho-mTOR expression in human glioblastoma microglia-macrophage cells

The glioblastoma (GBM) immune microenvironment is highly heterogeneous, and microglia may represent 30–70% of the entire tumor. However, the role of microglia and other specific immune populations is poorly characterized. Activation of mTOR signaling occurs in numerous human cancers and has roles in microglia-glioma cell interactions. We now show in human tumor specimens (42 patients), that 39% of tumor-associated microglial (TAM) cells express mTOR phosphorylated at Ser-2448; and similar mTOR activation is observed using a human microglia-glioma interaction paradigm. In addition, we confirm previous studies that microglia express urea and ARG1 (taken as M2 marker) in the presence of glioma cells, and this phenotype is down-regulated in the presence of a mTOR inhibitor. These results suggest that mTOR suppression in GBM patients might induce a reduction of the M2 phenotype expression in up to 40% of all TAMs. Since the M2 profile of microglial activation is believed to be associated with tumor progression, reductions in that phenotype may represent an additional anti-tumor mechanism of action of mTOR inhibitors, along with direct anti-proliferative activities

Designing cascades of electron transfer processes in multicomponent graphene conjugates

A novel family of nanocarbon-based materials was designed, synthesized, and probed within the context of charge-transfer cascades. We integrated electron-donating ferrocenes with light-harvesting/electron-donating (metallo)porphyrins and electron-accepting graphene nanoplates (GNP) into multicomponent conjugates. To control the rate of charge flow between the individual building blocks, we bridged them via oligo-p-phenyleneethynylenes of variable lengths by β-linkages and the Prato-Maggini reaction. With steady-state absorption, fluorescence, Raman, and XPS measurements we realized the basic physico-chemical characterization of the photo- and redox-active components and the multicomponent conjugates. Going beyond this, we performed transient absorption measurements and corroborated by single wavelength and target analyses that the selective (metallo)porphyrin photoexcitation triggers a cascade of charge transfer events, that is, charge separation, charge shift, and charge recombination, to enable the directed charge flow. The net result is a few nanosecond-lived charge-separated state featuring a GNP-delocalized electron and a one-electron oxidized ferrocenium

Plastic fiber design for THz generation through wavelength translation

We report on an all-fiber Terahertz (THz) radiation source by exploiting nonlinear parametric process in a theoretically designed microstructured-core double clad plastic fiber (MC-DCPF). The required phase-matching condition is satisfied through suitable tailoring of the fiber dispersion and nonlinear properties at the pump wavelength of a high power CO2 laser, with a CO laser of much lower power acting as a seed concomitantly. Our simulated results reveal that a THz radiation source at the frequency of ~ 3 THz could be realized with a 3-dB phase-matching band-width of 2.13 GHz in a 65 m long optimized MC-DCPF. Maximum power conversion efficiency >1% is realizable even after including the material loss