229 research outputs found
Extremely low-frequency spectroscopy in low-field nuclear magnetic resonance
We demonstrate a new phenomenon in nuclear magnetic resonance spectroscopy, in which nuclear spin transitions are induced by radio frequency irradiation at extremely low frequencies (of the order of a few Hz). Slow Rabi oscillations are observed between spin states of different exchange symmetry. These âforbiddenâ transitions are rendered weakly allowed by differential electronic shielding effects on the radio frequency field. We generate coherence between the singlet and triplet states of 15N-labeled nitrous oxide in solution, and estimate the scalar coupling between the two 15N nuclei with a precision of a few mHz
Long-lived States in an intrinsically disordered protein domain
Long-lived states (LLS) are relaxation-favoured eigenstates of J-coupled magnetic nuclei. LLS were measured, along with classical 1H and 15 N relaxation rate constants, in aminoacids of the N-terminal Unique domain of the c-Src kinase (USrc), which is disordered in vitro under physiological conditions. The relaxation rates of LLS are a probe for motions and interactions in biomolecules. LLS of the aliphatic protons of glycines, with lifetimes ca. four times longer than their spin-lattice relaxation times, are reported for the first time in an intrinsically disordered protein domain (IDP). LLS relaxation experiments were integrated with 2D spectroscopy methods, further adapting them for studies on proteins
Singlet NMR methodology in two-spin-1/2 systems
This paper discusses methodology developed over the past 12 years in order to access and manipulate singlet order in systems comprising two coupled spin-1/2 nuclei in liquid-state nuclear magnetic resonance. Pulse sequences that are valid for different regimes are discussed, and fully analytical proofs are given using different spin dynamics techniques that include product operator methods, the single transition operator formalism, and average Hamiltonian theory. Methods used to filter singlet order from byproducts of pulse sequences are also listed and discussed analytically. The theoretical maximum amplitudes of the transformations achieved by these techniques are reported, together with the results of numerical simulations performed using custom-built simulation code
Direct enhancement of nuclear singlet order by dynamic nuclear polarization
Hyperpolarized singlet order is available immediately after dissolution DNP, avoiding need for additional preparation steps. We demonstrate this procedure on a sample of [1,2â13C2]pyruvic aci
A Simple Route to Strong Carbon-13 NMR Signals Detectable for Several Minutes
Nuclear magnetic resonance (NMR) and magnetic resonance imaging (MRI) suffer from low sensitivity and limited nuclear spin memory lifetimes. Although hyperpolarization techniques increase sensitivity, there is also a desire to increase relaxation times to expand the range of applications addressable by these methods. Here, we demonstrate a route to create hyperpolarized magnetization in 13C nuclear spin pairs that last much longer than normal lifetimes by storage in a singlet state. By combining molecular design and low-field storage with para-hydrogen derived hyperpolarization, we achieve more than three orders of signal amplification relative to equilibrium Zeeman polarization and an order of magnitude extension in state lifetime. These studies use a range of specifically synthesized pyridazine derivatives and dimethyl p-tolyl phenyl pyridazine is the most successful, achieving a lifetime of about 190s in low-field, which leads to a 13C-signal that is visible for 10 minutes
The long-lived nuclear singlet state of 15N-nitrous oxide in solution
A 15N nuclear singlet lifetime of over 26 min has been observed in a solution of 15N2O, by using a field-cycling NMR pulse sequence. This observation suggests applications of hyperpolarized 15N2O in medical imaging and for flow and diffusion studies
Prediction of low-field nuclear singlet lifetimes with molecular dynamics and quantum-chemical property surface
Correction: Substituent interference on supramolecular assembly in urea gelators: synthesis, structure prediction and NMR
Correction for âSubstituent interference on supramolecular assembly in urea gelators: synthesis, structure prediction and NMRâ by Francesca Piana et al., Soft Matter, 2016, 12, 4034â4043
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