Nanoemulsion of Minthostachys verticillata essential oil. In-vitro evaluation of its antibacterial activity

Infectious diseases constitute a problem of great importance for animal and human health, as well as the increasing bacterial resistance to antibiotics. In this context, medicinal plants emerge as an effective alternative to replace the use antibiotics. The essential oil (EO) of Minthostachys verticillata (Griseb.) Epling (Lamiaceae) has demonstrated a strong antimicrobial activity. However, its instability and hydrophobicity under normal storage conditions are limitations to its use. Nanoemulsion technology is an excellent way to solubilize, microencapsulate, and protect this compound. This study aimed to obtain a nanoemulsion based on M. verticillata EO and evaluate its antibacterial activity against Staphylococcus aureus. The EO was obtained by steam distillation. Identification and quantification of their components were determined by GC-MS revealing that the dominated chemical group was oxygenated monoterpenes. Nanoemulsions (NE) were characterized by measuring pH, transmittance, separation percentage, release profile, and morphology. The effect of NE on the growth of S. aureus and cyto-compatibility was also evaluated. The results showed that NE containing a higher percentage of tween 20 exhibited higher stability with an approximated droplet size of 10 nm. The effect of encapsulation process was evaluated by GC-MS revealing that the volatile components in EO were no affected. After 24 h, 74.24 ± 0.75% of EO was released from NE and the antibacterial activity of EO was enhanced considerably by its encapsulation. The incubation of S. aureus with the NE and pure EO, show a bacterial growth inhibition of 58.87% ± 0.99 and 46.72% ± 3.32 (p < 0.05), respectively. In addition, nanoemulsión did not cause toxicity to porcine and equine red blood cells. The results obtained showed that NE could be a potential vehicle for M. verticillata EO with promissory properties to emerge as a tool for developing advanced therapies to control and combat infections.EEA Marcos JuárezFil: Cecchini, María Eugenia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Departamento de Microbiología e Inmunología. Laboratorio de Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología Ambiental y Salud. Universidad Nacional de Río Cuarto. Instituto de Biotecnología Ambiental y Salud; ArgentinaFil: Paoloni, C. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Anatomía Animal. Laboratorio de Biotecnología Animal; ArgentinaFil: Campra, Noelia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Departamento de Microbiología e Inmunología. Laboratorio de Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología Ambiental y Salud. Universidad Nacional de Río Cuarto. Instituto de Biotecnología Ambiental y Salud; ArgentinaFil: Picco, Natalia. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Anatomía Animal. Laboratorio de Biotecnología Animal; ArgentinaFil: Grosso, M. Carolina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Anatomía Animal. Laboratorio de Biotecnología Animal; ArgentinaFil: Soriano Perez, María Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; ArgentinaFil: Alustiza, Fabrisio Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; ArgentinaFil: Cariddi, Noelia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Departamento de Microbiología e Inmunología. Laboratorio de Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología Ambiental y Salud. Universidad Nacional de Río Cuarto. Instituto de Biotecnología Ambiental y Salud; ArgentinaFil: Bellingeri, Romina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Anatomía Animal. Laboratorio de Biotecnología Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados. Universidad Nacional de Río Cuarto. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; Argentin

Regulation of plasma membrane calcium ATPase (PMCA) by actin cytoskeleton

The Plasma Membrane Calcium ATPase (PMCA) is a calmodulin-modulated P-type ATPaseresponsible for the maintenance of low intracellular concentrations of Ca2+ in mosteukaryotic cells. Our group have previously shown that purified actin can exert a dualmodulation on the activity of Ca2+-ATPase 4b isoform (hPMCA4b): F-actin inhibits it whileshort actin oligomers may contribute to its activation. These studies had to be performedwith purified proteins given the nature of the biophysical and biochemical approachesused.On the other hand, in HEK293 human cells that overexpressed PMCA2w/b isoform, theactin depolymerization upon Cytochalasin D (CytD) treatment significantly increasedPMCA2-mediated Ca2+ extrusion and when F-actin was stabilized using jasplakinolide,PMCA2w/b activity was completely abolished.In order to assess whether the functional interaction between the hPMCA4 isoform and theactin cytoskeleton may be of physiological relevance, we decided to further characterize itin the context of a living cell by monitoring in real-time the changes in the actinpolymerization and cytosolic Ca2+ concentration ([Ca2+]cyt). For this, hPMCA4 isoform wastransiently expressed in HEK293T cells. The dynamics of [Ca2+]cyt was performed usingthe fluorescent probe Fluo-4 and studying the alterations in [Ca2+]cyt generated by Ca2+release from the endoplasmic reticulum, and by extracellular Ca2+ entry through store-operated Ca2+ channels. The dynamics of actin polymerization was performed transientlyexpressing LifeAct-Ruby.Results show that the alteration of actin polymerization by CytD treatment significantlyincreased hPMCA4 activity (102%). On the other hand, in absent of CytD, actinpolymerization dynamics did not change after TG stimulus, while after Ca2+ stimulus, anactin reorganization was observed. This reorganization takes place at the same times thatthe hPMCA4 increases its activity, suggesting that hPMCA4 may be activated by actindepolymerization in the cells.Fil: Vigil, Maximiliano Angel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Picco, María Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; ArgentinaFil: Rinaldi, Debora Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Rossi, Rolando Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Rey, Osvaldo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; ArgentinaFil: Rossi, Juan Pablo Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Ferreira Gomes, Mariela Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaXLIX Reunión Anual de la Sociedad Argentina de BiofísicaArgentinaSociedad Argentina de Biofísic

Experiencia sobre el proceso de salud-enfermedad-atención en pacientes con artritis reumatoidea pertenecientes a la comunidad Wichí de Misión Chaqueña “El Algarrobal”, Salta

Introducción: se ha reportado que la prevalencia de artritis reumatoidea (AR) en la comunidad Wichí representa la más alta informada por el Grupo Latinoamericano para el Estudio de las Enfermedades Reumáticas en los Pueblos Originarios (GLADERPO). El objetivo de este estudio fue describir la experiencia sobre el proceso de salud- enfermedad-atención de pacientes con AR de la comunidad Wichí de Misión Chaqueña “El Algarrobal”, Salta. Materiales y métodos: estudio narrativo. Diseño de corte etnográfico. Se realizaron entrevistas semiestructuradas y observaciones registradas. Se utilizaron guías de entrevistas y observación. Los aspectos incluidos fueron: concepción del proceso salud-enfermedad, percepción de la AR en la vida diaria, el acceso al sistema de salud, utilización de recursos tradicionales y de medicina tradicional.

El aula inclusiva como respuesta autopoiética y el personalismo

This paper deals with the question of inclusive education in relation to the explanation offered by Niklas Luhmann from his theory of systems. In order to do this, the paper will start from an approximation to the characterization of the fundamental principles of this author's theory. In the light of this position, and from the perspective of Personalism, the impact of this theory on the structuring and organization of the educational system in its inclusive model is analyzed, considering it as an autopoietic response of the common education model system.El presente trabajo aborda la cuestión de la educación inclusiva en relación a la explicación que ofrece Niklas Luhmann desde su teoría de los sistemas. Para esto se partirá desde una aproximación a la caracterización de los principios fundamentales de la teoría de este autor. A la luz de esta posición, y desde la perspectiva del personalismo, se analiza el impacto de esta teoría en la estructuración y organización del sistema educativo en su modalidad inclusiva, considerándola como una respuesta autopoiética del mismo sistema de modalidad común

Tecnologías para la gestión del conocimiento organizacional

n.d.Fil: Picco, Alicia - Facultad Ciencias Económicas y Estadística - Universidad Nacional de Rosario - Argentin

Trait-Anxiety and Belief Updating: Exploring the predictions of the negativity bias and contrast avoidance model.

Beliefs play a crucial role in shaping our behaviors and mental-health outcomes. Asymmetric belief updating refers to the phenomenon where desirable information is updated more readily than undesirable information. An essential feature of anxiety is threat-overestimation and a tendency to focus on the negative aspects of experience while avoiding sharp negative emotional contrasts. These two characteristics lead to different predictions concerning belief updating. One scenario would suggest a reduction in asymmetric update behavior, indicating negativity bias, while the other would indicate an increase in asymmetric update, indicating contrast avoidance. To test these two rival predictions, participants (n = 44) first completed trait-measures and then performed a belief update task. Moreover, memory for the information presented was assessed in the short-term and long-term. Skin conductance response was measured to assess arousal levels during the task. Overall, our findings revealed that higher levels of trait-anxiety predicted a greater integration of desirable information but not undesirable information. In contrast, trait-intolerance of uncertainty did not exhibit an association with update behavior. Skin conductance and memory were not associated with trait-measures. We discuss these results in line with the Contrast Avoidance Model of anxiety, in terms of avoidance of unexpected negative contrast and positive contrast induced by relief during belief updating

Oxidative damage and DNA damage in catle with hypocuprosis

La hipocuprosis bovina es una de las principales enfermedades nutricionales que afectan al ganado en pastoreo. Observaciones preliminares realizadas en bovinos hipocuprosos sugieren que el daño en la molécula de ADN podría ser una de las primeras consecuencias de la enfermedad. Dicho efecto podría verse relacionado con una disminución de la capacidad orgánica antioxidante. El presente trabajo pretende establecer la existencia y precocidad de aparición del daño en el ADN (medido mediante el empleo del ensayo cometa), y su vinculación con la capacidad orgánica antioxidante, valorada a través de la actividad Cu/Zn-superoxido dismutasa y a través del índice de peroxidación (IP) y composición de ácidos grasos de eritrocitos Cu-deficientes. Los resultados obtenidos demuestran un alto grado de asociación entre cupremia y daño en el ADN, concomitantes con un incremento en el porcentaje de ácidos grasos saturados en glóbulos rojos, indicando una alteración oxidativa. La misma no sería causada por la Cu/Zn-Superoxido dismutasa dada la lentitud en su afectación. En conclusión, la deficiencia de Cu provoca la producción precoz de daño en la molécula de ADN, participando probablemente en la génesis de dicho daño la alteración de la capacidad orgánica antioxidante, la cual no estaría directamente relacionada con la actividad SOD.Hypocuprosis is the most widespread mineral deficiency affecting grazing cattle. Preliminary reports suggest that DNA damage is one of the most precocious consecuences of copper deficiency. This effect might be related to a decrease in the organic antioxidant capacity. The present study was carried out to estabilish the existence and speed of DNA damage measured by the comet assay. Besides, its relation to the organic antioxidant capacity (measured by the activity of Cu/Zn- superoxide dismutase), the peroxidative index and fatty acid composition of eritrocytes were investigated. The results showed a high correlation between cupremia and DNA damage, with an increase in the saturated fatty acid composition of eritrocytes, suggesting the oxidative origin of the damage. However, the activity of Cu/Zn superoxide dismutase may not directly related to oxidative damage because a low diminishing. In conclusion, copper deficiency give rise to a precocious DNA damage, related to an alteration in the antioxidant capacity, which was not directly associated to a SOD activity.Facultad de Ciencias Veterinaria

Oxidative damage and DNA damage in catle with hypocuprosis

La hipocuprosis bovina es una de las principales enfermedades nutricionales que afectan al ganado en pastoreo. Observaciones preliminares realizadas en bovinos hipocuprosos sugieren que el daño en la molécula de ADN podría ser una de las primeras consecuencias de la enfermedad. Dicho efecto podría verse relacionado con una disminución de la capacidad orgánica antioxidante. El presente trabajo pretende establecer la existencia y precocidad de aparición del daño en el ADN (medido mediante el empleo del ensayo cometa), y su vinculación con la capacidad orgánica antioxidante, valorada a través de la actividad Cu/Zn-superoxido dismutasa y a través del índice de peroxidación (IP) y composición de ácidos grasos de eritrocitos Cu-deficientes. Los resultados obtenidos demuestran un alto grado de asociación entre cupremia y daño en el ADN, concomitantes con un incremento en el porcentaje de ácidos grasos saturados en glóbulos rojos, indicando una alteración oxidativa. La misma no sería causada por la Cu/Zn-Superoxido dismutasa dada la lentitud en su afectación. En conclusión, la deficiencia de Cu provoca la producción precoz de daño en la molécula de ADN, participando probablemente en la génesis de dicho daño la alteración de la capacidad orgánica antioxidante, la cual no estaría directamente relacionada con la actividad SOD.Hypocuprosis is the most widespread mineral deficiency affecting grazing cattle. Preliminary reports suggest that DNA damage is one of the most precocious consecuences of copper deficiency. This effect might be related to a decrease in the organic antioxidant capacity. The present study was carried out to estabilish the existence and speed of DNA damage measured by the comet assay. Besides, its relation to the organic antioxidant capacity (measured by the activity of Cu/Zn- superoxide dismutase), the peroxidative index and fatty acid composition of eritrocytes were investigated. The results showed a high correlation between cupremia and DNA damage, with an increase in the saturated fatty acid composition of eritrocytes, suggesting the oxidative origin of the damage. However, the activity of Cu/Zn superoxide dismutase may not directly related to oxidative damage because a low diminishing. In conclusion, copper deficiency give rise to a precocious DNA damage, related to an alteration in the antioxidant capacity, which was not directly associated to a SOD activity.Facultad de Ciencias Veterinaria

Vol. 1, n. 1 (2019): Persona, Cura, Territorio. Nuovi paradigmi educativi

Vol. 1, n. 1 (2019): Indirizzo di saluto della Senatrice Liliana Segre, P. 1 ; Mannese, E., Presentazione, P. 1 ; Iori, V. ; Editoriale-Il territorio e l’abitare: prendersi cura delle relazioni educative, P. 2 ; Balzano, V., Il volontariato come pratica di dono e relazione d’aiuto nella costruzione del progetto di vita, P. 10 ; Birbes, C., Verso un’abitanza sostenibile, P. 16 ; Castaldi, M.C., Progettare l’educativo nei contesti territoriali: premesse e operatività, P. 24 ; De Simone, M., Paesaggi di vita e paesaggi dell’educativo: un laboratorio clinico pedagogico con gli studenti universitari, P. 30 ; Gherardi, V., Leonardi, G., Scuola e territorio. Un progetto partecipato didattico culturale, P. 40 ; Giovannazzi, T., Sviluppo umano, cura, ambiente. Alcune riflessioni pedagogiche, P. 46 ; Iavarone, M.L., Varriale, L., Girardi, F., Ambra, F.V., Un esperimento di pedagogia civile. Il Master dell’Università Parthenope per “Educatori sportivi per la prevenzione del rischio”. P. 56 ; Lo Piccolo, A., Mingrino, M., Passaniti, V., Formazione umana e pedagogia della corporeità nell’era digitale, P. 68 ; Lombardi, M.G., Educare al territorio tra competenze e responsabilità, P. 78 ; Orlandini, L., Giunti, C., Educare alla territorialità attraverso l’approccio pedagogico del Service Learning, P. 82 ; Picco, M.E., Pedagogía y Personalismo, P. 95 ; Pignalberi, C., Direzione 2030: esperienze di cooperazione e partecipazione attiva alla rigenerazione educativa di comunità, P. 102 ; Pistillo, G., Alle origini della cura. Il sistema madre-bambino come paradigma del cambiamento e la dinamica del contatto, P. 116 ; Ricciardi, M., La pedagogia del lavoro: apprendimento generativo, innovazione e territorio per la formazione dei talenti, P. 126 ; Sirignano, F.M., Maddalena, S., El metodo autobiografico y la circularidad formativa, entre narraciones, emociones, contaminaciones, P. 135 ; Sottocorno, M., La Questione della Povertà Educativa. Prospettive Pedagogiche di Riflessione e di Intervento, P. 145 ; Vacchelli, O., Educazione alla territorialità ed etica della responsabilità, P. 152

Environmental and societal factors associated with COVID-19-related death in people with rheumatic disease: an observational study

Published by Elsevier Ltd.Background: Differences in the distribution of individual-level clinical risk factors across regions do not fully explain the observed global disparities in COVID-19 outcomes. We aimed to investigate the associations between environmental and societal factors and country-level variations in mortality attributed to COVID-19 among people with rheumatic disease globally. Methods: In this observational study, we derived individual-level data on adults (aged 18-99 years) with rheumatic disease and a confirmed status of their highest COVID-19 severity level from the COVID-19 Global Rheumatology Alliance (GRA) registry, collected between March 12, 2020, and Aug 27, 2021. Environmental and societal factors were obtained from publicly available sources. The primary endpoint was mortality attributed to COVID-19. We used a multivariable logistic regression to evaluate independent associations between environmental and societal factors and death, after controlling for individual-level risk factors. We used a series of nested mixed-effects models to establish whether environmental and societal factors sufficiently explained country-level variations in death. Findings: 14 044 patients from 23 countries were included in the analyses. 10 178 (72·5%) individuals were female and 3866 (27·5%) were male, with a mean age of 54·4 years (SD 15·6). Air pollution (odds ratio 1·10 per 10 μg/m3 [95% CI 1·01-1·17]; p=0·0105), proportion of the population aged 65 years or older (1·19 per 1% increase [1·10-1·30]; p<0·0001), and population mobility (1·03 per 1% increase in number of visits to grocery and pharmacy stores [1·02-1·05]; p<0·0001 and 1·02 per 1% increase in number of visits to workplaces [1·00-1·03]; p=0·032) were independently associated with higher odds of mortality. Number of hospital beds (0·94 per 1-unit increase per 1000 people [0·88-1·00]; p=0·046), human development index (0·65 per 0·1-unit increase [0·44-0·96]; p=0·032), government response stringency (0·83 per 10-unit increase in containment index [0·74-0·93]; p=0·0018), as well as follow-up time (0·78 per month [0·69-0·88]; p<0·0001) were independently associated with lower odds of mortality. These factors sufficiently explained country-level variations in death attributable to COVID-19 (intraclass correlation coefficient 1·2% [0·1-9·5]; p=0·14). Interpretation: Our findings highlight the importance of environmental and societal factors as potential explanations of the observed regional disparities in COVID-19 outcomes among people with rheumatic disease and lay foundation for a new research agenda to address these disparities.MAG is supported by the National Institute of Arthritis and Musculoskeletal and Skin Diseases (grant numbers K01 AR070585 and K24 AR074534 [JY]). KDW is supported by the Department of Veterans Affairs and the Rheumatology Research Foundation Scientist Development award. JAS is supported by the National Institute of Arthritis and Musculoskeletal and Skin Diseases (grant numbers K23 AR069688, R03 AR075886, L30 AR066953, P30 AR070253, and P30 AR072577), the Rheumatology Research Foundation (K Supplement Award and R Bridge Award), the Brigham Research Institute, and the R. Bruce and Joan M. Mickey Research Scholar Fund. NJP is supported by the National Institute of Arthritis and Musculoskeletal and Skin Diseases (T32-AR-007258). AD-G is supported by grants from the Centers for Disease Control and Prevention and the Rheumatology Research Foundation. RH was supported by the Justus-Liebig University Giessen Clinician Scientist Program in Biomedical Research to work on this registry. JY is supported by grants from the National Institutes of Health (K24 AR074534 and P30 AR070155).info:eu-repo/semantics/publishedVersio