11 research outputs found
Electrospun Thymosin Beta-4 Loaded PLGA/PLA Nanofiber/ Microfiber Hybrid Yarns for Tendon Tissue Engineering Application
Microfiber yarns (MY) have been widely employed to construct tendon tissue grafts. However, suboptimal ultrastructure and inappropriate environments for cell interactions limit their clinical application. Herein, we designed a modified electrospinning device to coat poly(lactic-co-glycolic acid) PLGA nanofibers onto polylactic acid (PLA) MY to generate PLGA/PLA hybrid yarns (HY), which had a well-aligned nanofibrous structure, resembling the ultrastructure of native tendon tissues and showed enhanced failure load compared to PLA MY. PLGA/PLA HY significantly improved the growth, proliferation, and tendon-specific gene expressions of human adipose derived mesenchymal stem cells (HADMSC) compared to PLA MY. Moreover, thymosin beta-4 (TĪ²4) loaded PLGA/PLA HY presented a sustained drug release manner for 28 days and showed an additive effect on promoting HADMSC migration, proliferation, and tenogenic differentiation. Collectively, the combination of TĪ²4 with the nano-topography of PLGA/PLA HY might be an efficient strategy to promote tenogenesis of adult stem cells for tendon tissue engineering
Prospective monitoring improves outcomes of primary total hip replacement: a cohort study
This is an Open Access article distributed under the terms of the Creative Commons Attribution Licens
3D printing of multilayered scaļ¬olds for rotator cuļ¬ tendon regeneration
Repairing massive rotator cuļ¬ tendon defects remains a challenge due to the high retear rate after surgical intervention. 3D printing has emerged as a promising technique that enables the fabrication of engineered tissues with heterogeneous structures and mechanical properties, as well as controllable microenvironments for tendon regeneration. In this study, we developed a new strategy for rotator cuļ¬ tendon repair by combining a 3D printed scaļ¬old of polylactic-co-glycolic acid (PLGA) with cell-laden collagen-ļ¬brin hydrogels. We designed and fabricated two types of scaļ¬olds: one featuring a separate layer-by-layer structure and another with a tri-layered structure as a whole. Uniaxial tensile tests showed that both types of scaļ¬olds had improved mechanical properties compared to single-layered PLGA scaļ¬olds. The printed scaļ¬old with collagen-ļ¬brin hydrogels effectively supported the growth, proliferation, and tenogenic diļ¬erentiation of human adipose-derived me-senchymal stem cells. Subcutaneous implantation of the multilayered scaļ¬olds demonstrated their excellent in vivo biocompatibility. This study demonstrates the feasibility of 3D printing multilayered scaļ¬olds for application in rotator cuļ¬ tendon regeneration
Adult Proximal Humerus Locking Plate for the Treatment of a Pediatric Subtrochanteric Femoral Nonunion: A Case Report
Nonunions of pediatric subtrochanteric femur fractures are exceedingly rare and have to date not been reported in the literature. We present the case of an 11-year-old boy who developed such a nonunion after open reduction internal fixation using a pediatric locked proximal femur plate. Using an adult proximal humerus locking plate, adequate proximal fixation of the nonunion was obtained. Furthermore, previously placed distal screw holes were safely bridged and the biomechanical environment around the nonunion site improved. Uneventful healing was possible with the use of adjuvant bone grafting. No short- or midterm complications occurred. Although other implants can certainly be adapted to a use different than that of its original design, the present case suggests that adult proximal humerus locking plates may be a safe option for revision surgery of the proximal pediatric femur
3D printing of multilayered scaļ¬olds for rotator cuļ¬ tendon regeneration
Repairing massive rotator cuļ¬ tendon defects remains a challenge due to the high retear rate after surgical intervention. 3D printing has emerged as a promising technique that enables the fabrication of engineered tissues with heterogeneous structures and mechanical properties, as well as controllable microenvironments for tendon regeneration. In this study, we developed a new strategy for rotator cuļ¬ tendon repair by combining a 3D printed scaļ¬old of polylactic-co-glycolic acid (PLGA) with cell-laden collagen-ļ¬brin hydrogels. We designed and fabricated two types of scaļ¬olds: one featuring a separate layer-by-layer structure and another with a tri-layered structure as a whole. Uniaxial tensile tests showed that both types of scaļ¬olds had improved mechanical properties compared to single-layered PLGA scaļ¬olds. The printed scaļ¬old with collagen-ļ¬brin hydrogels effectively supported the growth, proliferation, and tenogenic diļ¬erentiation of human adipose-derived me-senchymal stem cells. Subcutaneous implantation of the multilayered scaļ¬olds demonstrated their excellent in vivo biocompatibility. This study demonstrates the feasibility of 3D printing multilayered scaļ¬olds for application in rotator cuļ¬ tendon regeneration
Differential fracture healing resulting from fixation stiffness variability: a mouse model
Minimally invasive plate osteosynthesis for humeral shaft fractures: are results reproducible?
Minimally invasive plate osteosynthesis (MIPO) has been advocated as a safe approach to humeral shaft fracture management. We evaluated the reproducibility of this technique in a regional hospital. Thirty-five patients underwent MIPO of humerus shaft fractures. Fifteen patients had an open fracture, six a preoperative radial nerve palsy, and nine a concomitant thoracic, musculoskeletal or vascular injury. At an average 12-month follow-up, 91% of fractures healed after a mean of 12Ā weeks (range, 8ā16). Two infections occurred. Final alignment averaged 4Ā° of varus (range, 5Ā° of valgus to 20Ā° of varus). Active elbow ROM averaged 114Ā° (range, 60ā135Ā°) and was less than 100Ā° in nine elbows. Five of six preoperative radial nerve injuries recovered spontaneously. Healing and infection rates in this study are consistent with those reported in the literature. Lower elbow ROM and higher fracture angulation at healing were nevertheless found. MIPO is technically demanding and requires adequate intraoperative imaging and surgical experience in order to obtain adequate fracture alignment. Brachialis muscle scarring and inadequate postoperative rehabilitation may be involved in limited elbow range of motion