Cx3cr1-deficient microglia exhibit a premature aging transcriptome.

CX3CR1, one of the highest expressed genes in microglia in mice and humans, is implicated in numerous microglial functions. However, the molecular mechanisms underlying Cx3cr1 signaling are not well understood. Here, we analyzed transcriptomes of Cx3cr1-deficient microglia under varying conditions by RNA-sequencing (RNA-seq). In 2-mo-old mice, Cx3cr1 deletion resulted in the down-regulation of a subset of immune-related genes, without substantial epigenetic changes in markers of active chromatin. Surprisingly, Cx3cr1-deficient microglia from young mice exhibited a transcriptome consistent with that of aged Cx3cr1-sufficient animals, suggesting a premature aging transcriptomic signature. Immunohistochemical analysis of microglia in young and aged mice revealed that loss of Cx3cr1 modulates microglial morphology in a comparable fashion. Our results suggest that CX3CR1 may regulate microglial function in part by modulating the expression levels of a subset of inflammatory genes during chronological aging, making Cx3cr1-deficient mice useful for studying aged microglia

The Grizzly, October 16, 1990

Olin Walkway Changes the Face of Ursinus • Congressional Candidate Lewis Du Pont Smith Spurs Debate • The Minority Student Union: A Step Toward Diversity • USEAC Conference Bolsters Environmental Activism • Father Changes • Reflecting on Rosen • New Hang-out • The Replacements • Upcoming Berman • Cross Country Runs On • Soccer Wins Two • Netters Upset • Hockey Splits • Grubb Predicts Again • Football Refuses To Give Up • Letters: Complain, but Don\u27t Steal; Positive Pledging; Wismer Food: Quick Service and Healthy Variety • A Contradiction in American Beliefs • This is America? • A Spectacular Devicehttps://digitalcommons.ursinus.edu/grizzlynews/1261/thumbnail.jp

Review of Curriculum Content and Programs for Anaerobic Digestion Education and Training

The development of the anaerobic digestion (AD) industry requires financial, physical and human resources. In New York State, financial and physical resources have been mobilized to finance and build ten anaerobic digesters on dairy farms. The objective of this report is to make recommendations for the development of human resources as the groundwork for the creation of technical AD coursework which can lead to the development of a robust and comprehensive anaerobic digestion industry

Perceptions of pharmacists on the quality of automated blood pressure devices: a national survey

A recent study found that only 23.8% of blood pressure (BP) devices available for purchase from Australian pharmacies were validated for accuracy. The extent to which pharmacists are aware of this, and other issues related to the accuracy of BP devices, is not known and gathering this information was the aim of this study. An online survey of Australian pharmacists was distributed via the Pharmaceutical Society of Australia between 1 October and 25 November 2020. Questions were focused on the views of pharmacists related to the accuracy of BP devices. Two hundred and ten pharmacists completed the survey. The accuracy of BP devices sold by pharmacists was considered ‘quite’ or ‘extremely important’ to most respondents (94%). However, most respondents (90%) were unaware that less than one-quarter of BP devices sold by Australian pharmacies were validated, and this was ‘quite’ or ‘extremely surprising’ to many (69%). Many respondents (64%) associated a particular brand of BP device with greater accuracy. There was low awareness on proper ways to identify accurate BP devices, such as checking reputable online databases (43%). BP devices were stocked in respondents’ pharmacies based on perceived quality (50%), accuracy (40%), or as determined by the pharmacy chain (36%). In conclusion, providing accurate BP devices to consumers is important to pharmacists, but they were generally unaware that most devices available from pharmacies were not validated for accuracy. Pharmacist education, alongside advocacy for policies including regulations and strategic action, is required to ensure only validated BP devices are sold in Australia

Testing Alternative Cultural Explanations of Managers’ Values across the U.S.-Canada Border

Research about the implications that cultural differences have for managers in different parts of Anglophone Canada and the United States, like management studies of regional culture throughout the world, have been based on varied, narrowly focused theories and have reached varied conclusions. Here, we identify and compare theories about immigrant group characteristics and contemporary socioeconomic characteristics that figure strongly in research comparing Canada, the United States, and their regions. We summarize the predictions that each immigration theory and each socioeconomic characteristic makes for the two implications of culture that are most prominent in regional studies of North America – self-reliance and deference to authority. We conclude with thoughts about the potential contributions of culture research about North American regions along with theories of culture emergence and change that need to be revised or added to them to advance research about cultural regions of Europe

Chlorhexidine-coated surgical gloves influence the bacterial flora of hands over a period of 3 hours

Abstract Background The risk of SSI increases in the presence of foreign materials and may be caused by organisms with low pathogenicity, such as skin flora derived from hands of surgical team members in the event of a glove breach. Previously, we were able to demonstrate that a novel antimicrobial surgical glove coated chlorhexidine-digluconate as the active ingredient on its inner surface was able to suppress surgeons’ hand flora during operative procedures by a magnitude of 1.7 log10 cfu/mL. Because of the clinical design of that study, we were not able to measure the full magnitude of the possible antibacterial suppression effect of antimicrobial gloves over a full 3 h period. Methods The experimental procedure followed the method for assessment of the 3-h effects of a surgical hand rub’s efficacy to reduce the release of hand flora as described in the European Norm EN 12791. Healthy volunteers tested either an antimicrobial surgical glove or non-antimicrobial surgical latex gloves in a standardized laboratory-based experiment over a wear time of 3 h. Results Wearing antimicrobial surgical glove after a surgical hand rub with 60% (v/v) n-propanol resulted in the highest 3-h reduction factor of 2.67 log10. Non-antimicrobial surgical gloves demonstrated significantly lower (p ≤ 0.01) 3-h reduction factors at 1.96 log10 and 1.68 log10, respectively. Antibacterial surgical gloves are able to maintain a sustainable bacterial reduction on finger tips in a magnitude of almost 3 log10 (log10 2.67 cfu) over 3 h wear time. Conclusion It was demonstrated that wear of an antibacterial surgical glove coated with chlorhexidine-digluconate is able to suppress resident hand flora significantly over a period of 3-h