Paper-based microfluidic devices on the crime scene: a simple tool for rapid estimation of post-mortem interval using vitreous humour

CAPES - COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIORCNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE GOIÁS - FAPEGFAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DEThis paper describes for the first time the use of paper-based analytical devices at crime scenes to estimate the post-mortem interval (PMI), based on the colorimetric determination of Fe2+ in vitreous humour (VH) samples. Experimental parameters such as9746974CAPES - COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIORCNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE GOIÁS - FAPEGFAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DECAPES - COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIORCNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE GOIÁS - FAPEGFAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE3363/201488881.062204/2014-01448089/2014-9308140/2016-8sem informação2016/07384-7This study was supported by CAPES (grants 3363/2014 and 88881.062204/2014-01), CNPq (grants 448089/2014-9 and 308140/2016-8), FAPEG, FAPESP (grant 2016/07384-7) and INCTBio (grant 465389/2014-7). CAPES and CNPq are also acknowledged for the scholarships

Mammals, Serra da Concórdia, state of Rio de Janeiro, Brazil.

The Atlantic Forest in the state of Rio de Janeiro is extremely fragmented and reduced in its originalextension. Although there are a great number of studies related to the biome, few lists are available for this region. Thepresent study seeks to get information on the mammals of a remnant forest in Serra da Concórdia, an area from whichno information is currently available. The rapid mammal's survey was accomplished by use of pitfalls, live traps,transects, mist nets, and camera traps. We recorded 37 terrestrial species that represent 22.3 % of Rio de Janeiro statemammal species. Among the recorded species, six are endemic of the Atlantic Forest, one is endemic of the state, andeight are in the state list of threatened or presumably threatened species. The area presents great mammal richness andan increasing effort will probably reveal the presence of additional species

In vitro induced pluripotency from urine-derived cells in porcine.

Methods: The UDCs were reprogrammed in vitro using human or murine octamer-binding transcription factor 4 (OCT4), SRY-box2 (SOX2), Kruppel-like factor 4 (KLF4), and C-MYC, and cultured with basic fibroblast growth factor (bFGF) supplementation. To characterize the putative porcine iPSCs three clonal lineages were submitted to immunocytochemistry for alkaline phosphatase (AP), OCT4, SOX2, NANOG, TRA1 81 and SSEA 1 detection. Endogenous transcripts related to the pluripotency (OCT4, SOX2 and NANOG) were analyzed via reverse transcription quantitative real-time polymerase chain reaction in different time points during the culture, and all three lineages formed embryoid bodies (EBs) when cultured in suspension without bFGF supplementation. Results: The UDCs were isolated from swine urine samples and when at passage 2 submitted to in vitro reprogramming. Colonies of putative iPSCs were obtained only from UDCs transduced with the murine factors (mOSKM), but not from human factors (hOSKM). Three clonal lineages were isolated and further cultured for at least 28 passages, all the lineages were positive for AP detection, the OCT4, SOX2, NANOG markers, albeit the immunocytochemical analysis also revealed heterogeneous phenotypic profiles among lineages and passages for NANOG and SSEA1, similar results were observed in the abundance of the endogenous transcripts related to pluripotent state. All the clonal lineages when cultured in suspension without bFGF were able to form EBs expressing ectoderm and mesoderm layers transcripts. Conclusion: For the first time UDCs were isolated in the swine model and reprogrammed into a pluripotent-like state, enabling new numerous applications in both human or veterinary regenerative medicine

Amazonian forest degradation must be incorporated into the COP26 agenda

Nations will reaffirm their commitment to reducing greenhouse gas (GHG) emissions during the 26th United Nations Climate Change Conference (COP26; www.ukcop26.org), in Glasgow, Scotland, in November 2021. Revision of the national commitments will play a key role in defining the future of Earth’s climate. In past conferences, the main target of Amazonian nations was to reduce emissions resulting from land-use change and land management by committing to decrease deforestation rates, a well-known and efficient strategy1,2. However, human-induced forest degradation caused by fires, selective logging, and edge effects can also result in large carbon dioxide (CO2) emissions1,2,3,4,5, which are not yet explicitly reported by Amazonian countries. Despite its considerable impact, forest degradation has been largely overlooked in previous policy discussions5. It is vital that forest degradation is considered in the upcoming COP26 discussions and incorporated into future commitments to reduce GHG emissions

Synthesis and prospective study of the use of thiophene thiosemicarbazones as signalling scaffolding for the recognition of anions

A family of phenyl-thiosemicarbazone dyes have been prepared and their interactions with anions monitorized via UV-Vis, fluorescence and 1H NMR titrations. Additionally quantum chemical calculations and electrochemical studies completed the studies carried out. The phenyl-thiosemicarbazone dyes show a modulation of their hydrogen-bonding and electron-donating capabilities as a function of the chemical groups attached and display two different chromo-fluorogenic responses towards anions in acetonitrile solutions. The more basic anions fluoride and cyanide are able to induce the dual coordination-deprotonation processes for all the receptors studied, whereas acetate only interacts with receptors 2, 3, 6, 7, 8, 9 and dihydrogen phosphate displays sensing features only with the more acidic receptors 6. Coordinative hydrogen bonding interactions is indicated by a small bathochromic shift, whilst deprotonation results in the appearance of a new band at ca. 400-450 nm corresponding to a colour change from colourless-yellow to yellow-red depending on the receptor. In the emission fluorescence, hydrogen bonding interaction is visible through the enhancement of the emission band, whereas deprotonation induced the growth of a new red-shifted emission. The chromo-fluorogenic behaviour could be explained on the basis of the deprotonation tendency of the binding sites and the proton affinity of the anions. PM3 and 1H NMR calculations are in agreement with the existence of the dual complexation-deprotonation process, whereas both studies are in discrepancy in relation to which is the proton involved in the deprotonation. Electrochemical studies carried with receptor 3 showed a quite complex redox behaviour and anodic shifts of the reduction peaks in the presence of the basic anions fluoride, cyanide and acetate.Fundação para a Ciência e a Tecnologia (FCT