THE EFFECT OF HEAVY METAL CONTAMINATION ON ESTUARINE BENTHIC FAUNA AT VARYING LEVELS OF BIOLOGICAL ORGANISATION

Biological monitoring techniques, selected to cover increasing levels of biological organisation (cellular, individual, population and community), were compared and contrasted to determine the relative sensitivities of these approaches to heavy metal contammation. The study was centred on the Fal Estuary in Cornwall, an ideal experimental field site-with well documented heavy metal contamination resulting firom the long-term discharge of waste from a local tin mine. Five creeks leadmg from the estuary differ dramatically in the concentrations of heavy metals in the sediments, but little in natural environmental variables (sediment granulometry, salinity etc.). The contaminant induced damage was evaluated at the cellular level in Mytilus edulis by the Neutral Red Assay to detect lysosomal membrane damage. Parameters of the energy budget of this species were determined to estmiate 'Scope for Growth' of mdividuals. along with a Larval Survival assay to estabUsh their reproductive and developmental success. At the population level. Cohort Growth studies were undertaken using the polychaete Nephtys hombergi. At the community level, data firom an extensive three year survey of benthic macrofauna structure were analysed. In addition to investigating biological parameters, contammant levels in sedunent and tissue were determuied using Atomic Absorption Spectrommetry. All techniques, except the population level, detected the extreme pollution of the most contaminated creek, Restronguet, but did not accurately reflect the known metal gradient. However, community studies established distinct macrofaunal assemblages between both sites and years. Techniques were compared and validated in a novel approach using the program BIOENV, with the multivariate analysis package PRIMER (Plymouth Routines in Multivariate Ecological Research). The cellular assay correlated reasonably well agamst the heavy metal levels, the larval survival assay correlated well to the community structure. However, the community structure approach was the most sensitive and relevant monitoring method to determme the long-term contamination of the Fal Estuary.Plymouth Marine Laborator

Identifying Very Metal-Rich Stars with Low-Resolution Spectra: Finding Planet-Search Targets

We present empirical calibrations that estimate stellar metallicity, effective temperature and surface gravity as a function of Lick/IDS indices. These calibrations have been derived from a training set of 261 stars for which (1) high-precision measurements of [Fe/H], T_eff and log g have been made using spectral-synthesis analysis of HIRES spectra, and (2) Lick indices have also been measured. Our [Fe/H] calibration, which has precision 0.07 dex, has identified a number of bright (V < 9) metal-rich stars which are now being screened for hot Jupiter-type planets. Using the Yonsei-Yale stellar models, we show that the calibrations provide distance estimates accurate to 20% for nearby stars. This paper outlines the second tier of the screening of planet-search targets by the N2K Consortium, a project designed to identify the stars most likely to harbor extrasolar planets. Discoveries by the N2K Consortium include the transiting hot Saturn HD 149026 b (Sato et al. 2005, astro-ph/0507009) and HD 88133 b (Fischer et al. 2005). See Ammons et al. (2005, In Press) for a description of the first tier of N2K metallicity screening, calibrations using broadband photometry.Comment: Accepted for publication in the Astrophysical Journa

Communicating the uncertainty in estimated greenhouse gas emissions from agriculture

In an effort to mitigate anthropogenic effects on the global climate system, industrialised countries are required to quantify and report, for various economic sectors, the annual emissions of greenhouse gases from their several sources and the absorption of the same in different sinks. These estimates are uncertain, and this uncertainty must be communicated effectively, if government bodies, research scientists or members of the public are to draw sound conclusions. Our interest is in communicating the uncertainty in estimates of greenhouse gas emissions from agriculture to those who might directly use the results from the inventory. We tested six methods of communication. These were: a verbal scale using the IPCC calibrated phrases such as ‘likely’ and ‘very unlikely’; probabilities that emissions are within a defined range of values; confidence intervals for the expected value; histograms; box plots; and shaded arrays that depict the probability density of the uncertain quantity. In a formal trial we used these methods to communicate uncertainty about four specific inferences about greenhouse gas emissions in the UK. Sixty four individuals who use results from the greenhouse gas inventory professionally participated in the trial, and we tested how effectively the uncertainty about these inferences was communicated by means of a questionnaire. Our results showed differences in the efficacy of the methods of communication, and interactions with the nature of the target audience. We found that, although the verbal scale was thought to be a good method of communication it did not convey enough information and was open to misinterpretation. Shaded arrays were similarly criticised for being open to misinterpretation, but proved to give the best impression of uncertainty when participants were asked to interpret results from the greenhouse gas inventory. Box plots were most favoured by our participants largely because they were particularly favoured by those who worked in research or had a stronger mathematical background. We propose a combination of methods should be used to convey uncertainty in emissions and that this combination should be tailored to the professional grou

Contrasting Responses of Rhizosphere Bacterial, Fungal, Protist and Nematode Communities to Nitrogen Fertilisation and Crop Genotype in Field Grown Oilseed Rape (Brassica napus)

© 2021 Picot, Hale, Hilton, Teakle, Schäfer, Huang, Perryman, West and Bending. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). https://creativecommons.org/licenses/by/4.0/The rhizosphere microbiome is considered to play a key role in determining crop health. However, current understanding of the factors which shape assembly and composition of the microbiome is heavily biased toward bacterial communities, and the relevance for other microbial groups is unclear. Furthermore, community assembly is determined by a variety of factors, including host genotype, environment and agricultural management practices, and their relative importance and interactions remain to be elucidated. We investigated the impact of nitrogen fertilization on rhizosphere bacterial, fungal, nematode and protist communities of 10 contrasting oilseed rape genotypes in a field experiment. We found significant differences in the composition of bacteria, fungi, protist and nematode communities between the rhizosphere and bulk soil. Nitrogen application had a significant but weak effect on fungal, bacterial, and protist community composition, and this was associated with increased relative abundance of a complex of fungal pathogens in the rhizosphere and soil, including Mycosphaerella sp. and Leptosphaeria sp. Network analysis showed that nitrogen application had different effects on microbial community connectivity in the soil and rhizosphere. Crop genotype significantly affected fungal community composition, with evidence for a degree of genotype specificity for a number of pathogens, including L. maculans, Alternaria sp., Pyrenopeziza brassicae, Olpidium brassicae, and L. biglobosa, and also potentially beneficial Heliotales root endophytes. Crop genotype had no significant effect on assembly of bacteria, protist or nematode communities. There was no relationship between genetic distance of crop genotypes and the extent of dissimilarity of rhizosphere microbial communities. Field disease assessment confirmed infection of crops by Leptosphaeria sp., P. brassicae, and Alternaria sp., indicating that rhizosphere microbiome sequencing was an effective indicator of plant health. We conclude that under field conditions soil and rhizosphere nutrient stoichiometry and crop genotype are key factors determining crop health by influencing the infection of roots by pathogenic and mutualistic fungal communities, and the connectivity and stability of rhizosphere microbiome interaction networks.Peer reviewedFinal Published versio

An Improved uvby-Metallicity Calibration for Metal-Rich Stars

We present an improved uvby-metallicity relation calibrated for F, G, and early K dwarfs, and an analogous uvby-T(eff) relation, both derived using a Levenberg-Marquardt minimization scheme. Our calibrations are based on 1533 stars which appear in both the Cayrel de Strobel (2001) metallicity compilation, and in the Hauck-Mermilliod (1998) catalog of uvby photometry. We also examine the speculative possibility of using uvby photometry to produce a uvby-planeticity calibration. We conclude that while there is likely no strong photometric indicator of the presence or absence of short-period planets, the possibility of a spectroscopic indicator of planeticity is well worth examining.Comment: 9 pages, 4 figures, accepted in ApJ Letter

A Spitzer IRS Study of Debris Disks Around Planet-Host Stars

Since giant planets scatter planetesimals within a few tidal radii of their orbits, the locations of existing planetesimal belts indicate regions where giant planet formation failed in bygone protostellar disks. Infrared observations of circumstellar dust produced by colliding planetesimals are therefore powerful probes of the formation histories of known planets. Here we present new Spitzer IRS spectrophotometry of 111 Solar-type stars, including 105 planet hosts. Our observations reveal 11 debris disks, including two previously undetected debris disks orbiting HD 108874 and HD 130322. Combining our 32 micron spectrophotometry with previously published MIPS photometry, we find that the majority of debris disks around planet hosts have temperatures in the range 60 < T < 100 K. Assuming a dust temperature T = 70 K, which is representative of the nine debris disks detected by both IRS and MIPS, we find that debris rings surrounding Sunlike stars orbit between 15 and 240 AU, depending on the mean particle size. Our observations imply that the planets detected by radial-velocity searches formed within 240 AU of their parent stars. If any of the debris disks studied here have mostly large, blackbody emitting grains, their companion giant planets must have formed in a narrow region between the ice line and 15 AU.Comment: Accepted for publication in the Astronomical Journal. 14 pages, including five figures and two table

The N2K Consortium. VII. Atmospheric Parameters of 1907 Metal-Rich Stars: Finding Planet-Search Targets

We report high-precision atmospheric parameters for 1907 stars in the N2K low-resolution spectroscopic survey, designed to identify metal-rich FGK dwarfs likely to harbor detectable planets. 284 of these stars are in the ideal temperature range for planet searches, T_eff <= 6000K, and have a 10% or greater probability of hosting planets based on their metallicities. The stars in the low-resolution spectroscopic survey should eventually yield >60 new planets, including 8-9 hot Jupiters. Short-period planets have already been discovered orbiting the survey targets HIP 14801 and HD 149143.Comment: Accepted for publication in Astrophysical Journal Supplements. 24 pages, including 8 figures and one table. Two more tables will appear in ApJS online editio

A patient safety toolkit for family practices

Objectives: Major gaps remain in our understanding of primary care patient safety. We describe a toolkit for measuring patient safety in family practices. Methods: Six tools were used in 46 practices. These tools were: NHS Education for Scotland Trigger Tool, NHS Education for Scotland Medicines Reconciliation Tool, Primary Care Safequest, Prescribing Safety Indicators, PREOS-PC, and Concise Safe Systems Checklist. Results: PC-Safequest showed that most practices had a well-developed safety climate. However, the Trigger Tool revealed that a quarter of events identified were associated with moderate or substantial harm, with a third originating in primary care and avoidable. Although medicines reconciliation was undertaken within 2 days in >70% of cases, necessary discussions with a patient/carer did not always occur. The prescribing safety indicators identified 1,435 instances of potentially hazardous prescribing or lack of recommended monitoring (from 92,649 patients). The Concise Safe Systems Checklist found that 25% of staff thought their practice provided inadequate follow-up for vulnerable patients discharged from hospital and inadequate monitoring of non-collection of prescriptions. Most patients had a positive perception of the safety of their practice although 45% identified at least one safety problem in the past year. Conclusions: Patient safety is complex and multidimensional. The Patient Safety Toolkit is easy to use and hosted on a single platform with a collection of tools generating practical and actionable information. It enables family practices to identify safety deficits that they can review and change procedures to improve their patient safety across a key sets of patient safety issues

Patients’ evaluations of patient safety in English general practices: a cross-sectional study

Background: The frequency and nature of safety problems and harm in general practices has previously relied on information supplied by health professionals, and scarce attention has been paid to experiences of patients. Aim: To examine patient-reported experiences and outcomes of patient safety in Primary Care in England. Design and Setting: Cross-sectional study in 45 general practices. Method: A postal version of the Patient Reported Experiences and Outcomes of Safety in Primary Care (PREOS-PC) questionnaire was sent to a random sample of 6,736 patients. Main outcome measures included “practice activation” (what does the practice do to create a safe environment); “patient activation” (how pro-active are patients in ensuring safe healthcare delivery); “experiences of safety events” (safety errors); “outcomes of safety” (harm); and “overall perception of safety” (how safe do patients rate their practice). Results: 1,244 patients (18.4%) returned completed questionnaires. Scores were high for “practice activation” (mean (standard error) = 80.4 out of 100 (2.0)) and low for “patient activation” (26.3 out of 100 (2.6)). A substantial proportion of patients (45%) reported having experienced at least one safety problem in the previous 12 months, mostly related to appointments (33%), diagnosis (17%), patient-provider communication (15%), and coordination between providers (14%). 221 patients (23%) reported some degree of harm in the previous 12 months. The overall assessment of the level of safety of their practices was generally high (86.0 out of 100 (16.8)). Conclusion: Priority areas for patient safety improvement in general practices in England include appointments, diagnosis, communication, coordination and patient activation