Deficits in social behavioral tests in a mouse model of alternating hemiplegia of childhood

Social behavioral deficits have been observed in patients diagnosed with alternating hemiplegia of childhood (AHC), rapid-onset dystonia-parkinsonism and CAPOS syndrome, in which specific missense mutations in ATP1A3, encoding the Na+, K+-ATPase α3 subunit, have been identified. To test the hypothesis that social behavioral deficits represent part of the phenotype of Na+, K+-ATPase α3 mutations, we assessed the social behavior of the Myshkin mouse model of AHC, which has an I810N mutation identical to that found in an AHC patient with co-morbid autism. Myshkin mice displayed deficits in three tests of social behavior: nest building, pup retrieval and the three-chamber social approach test. Chronic treatment with the mood stabilizer lithium enhanced nest building in wild-type but not Myshkin mice. In light of previous studies revealing a broad profile of neurobehavioral deficits in the Myshkin model – consistent with the complex clinical profile of AHC – our results suggest that Na+, K+-ATPase α3 dysfunction has a deleterious, but nonspecific, effect on social behavior. By better defining the behavioral profile of Myshkin mice, we identify additional ATP1A3-related symptoms for which the Myshkin model could be used as a tool to advance understanding of the underlying neural mechanisms and develop novel therapeutic strategies

More than 10,000 pre-Columbian earthworks are still hidden throughout Amazonia.

This is the author accepted manuscript. The final version is available from the American Association for the Advancement of Science via the DOI in this recordData and materials availability: Data from publicly available sources are cited in the supplementary materials. Other data and computer codes used in the analysis are publicly available at Zenodo repositoryIndigenous societies are known to have occupied the Amazon basin for more than 12,000 years, but the scale of their influence on Amazonian forests remains uncertain. We report the discovery, using LIDAR (light detection and ranging) information from across the basin, of 24 previously undetected pre-Columbian earthworks beneath the forest canopy. Modeled distribution and abundance of large-scale archaeological sites across Amazonia suggest that between 10,272 and 23,648 sites remain to be discovered and that most will be found in the southwest. We also identified 53 domesticated tree species significantly associated with earthwork occurrence probability, likely suggesting past management practices. Closed-canopy forests across Amazonia are likely to contain thousands of undiscovered archaeological sites around which pre-Columbian societies actively modified forests, a discovery that opens opportunities for better understanding the magnitude of ancient human influence on Amazonia and its current state.Coordination of Superior Level Staff Improvement under the Academic Excellence Program (CAPES/PROEX)Coordination of Superior Level Staff Improvement under the Academic Excellence Program (CAPES/PROEX)Coordination of Superior Level Staff Improvement under the Academic Excellence Program (CAPES/PROEX)National Council for Scientific and Technological Development (CNPQ)National Council for Scientific and Technological Development (CNPQ)National Council for Scientific and Technological Development (CNPQ)European Research CouncilSão Paulo Research Foundation (FAPESP)Amazon FundSão Paulo Research Foundation (FAPESP)PVEMEC/MCTI/CAPES/CNPq/FAPEuropean Union’s Horizon 2020European Union’s Horizon 2020CAPESANRMCT/CNPq/CT-INFRA/GEOMAMCT/CNPq/CT-INFRA/GEOMACAPES/PDSECAPES/FapespaCNPqFAPESPCNPq/CAPES/FAPS/BC-NewtonFAPEMATRoyal Society GCRF International Collaboration AwardNSF/DEBCNPQ/PQNatural Environment Research Council (NERC)Natural Environment Research Council (NERC)Natural Environment Research Council (NERC)Natural Environment Research Council (NERC)Natural Environment Research Council (NERC)Natural Environment Research Council (NERC)Natural Environment Research Council (NERC)Gordon and Betty Moore Foundatio

More than 10,000 pre-Columbian earthworks are still hidden throughout Amazonia

Indigenous societies are known to have occupied the Amazon basin for more than 12,000 years, but the scale of their influence on Amazonian forests remains uncertain. We report the discovery, using LIDAR (light detection and ranging) information from across the basin, of 24 previously undetected pre-Columbian earthworks beneath the forest canopy. Modeled distribution and abundance of large-scale archaeological sites across Amazonia suggest that between 10,272 and 23,648 sites remain to be discovered and that most will be found in the southwest. We also identified 53 domesticated tree species significantly associated with earthwork occurrence probability, likely suggesting past management practices. Closed-canopy forests across Amazonia are likely to contain thousands of undiscovered archaeological sites around which pre-Columbian societies actively modified forests, a discovery that opens opportunities for better understanding the magnitude of ancient human influence on Amazonia and its current state

More than 10,000 pre-Columbian earthworks are still hidden throughout Amazonia

Indigenous societies are known to have occupied the Amazon basin for more than 12,000 years, but the scale of their influence on Amazonian forests remains uncertain. We report the discovery, using LIDAR (light detection and ranging) information from across the basin, of 24 previously undetected pre-Columbian earthworks beneath the forest canopy. Modeled distribution and abundance of large-scale archaeological sites across Amazonia suggest that between 10,272 and 23,648 sites remain to be discovered and that most will be found in the southwest. We also identified 53 domesticated tree species significantly associated with earthwork occurrence probability, likely suggesting past management practices. Closed-canopy forests across Amazonia are likely to contain thousands of undiscovered archaeological sites around which pre-Columbian societies actively modified forests, a discovery that opens opportunities for better understanding the magnitude of ancient human influence on Amazonia and its current state

Data from: Over 10,000 Pre-Columbian earthworks are still hidden throughout Amazonia

Dataset: This set of data and R computer codes were used to create the predictive model, figures, and develop analysis on the manuscript "Over 10,000 Pre-Columbian earthworks are still hidden throughout Amazonia" submitted to Science journal as a research article (DOI: ...ade2541). Please read the materials and methods sections on the manuscript supplementary materials, along with the data provided in the "Database" folder, to ensure reproducibility. Earthwork Predictive Model: The Inhomogeneous Poisson Process (IPP) model fit was performed using the 'fit_bayesPO' function of the 'bayesPO' library in R version 4.0.2. The model was developed by the author of the package Guido Alberti Moreira. Figures: Figures created from R computer codes presented on the Main text are inside the "MainText_figures" folder, and Supplementary material figures are inside the "SuppMaterial_figures" folder. Please utilize the instructions in the supplementary material in conjunction with the data in the "database" folder to ensure reproducibility. Dataset usage: It is free to use, but if you use this dataset in your work, please make sure to cite the repository and our paper properly. We also welcome users to invite us for collaboration. For the use of this dataset, please cite: Peripato, V. et al. Data from: Over 10,000 Pre-Columbian earthworks are still hidden throughout Amazonia (2023). DOI: 10.5281/zenodo.7750985. https://doi.org/10.5281/zenodo.775098