Effect of an acrylic resin combined with an antimicrobial polymer on biofilm formation

OBJECTIVES: The purpose of this study was to evaluate the antimicrobial activity of an acrylic resin combined with an antimicrobial polymer poly (2-tert-butylaminoethyl) methacrylate (PTBAEMA) to inhibit Staphylococcus aureus, Streptococcus mutans and Candida albicans biofilm formation. MATERIAL AND METHODS: Discs of a heat-polymerized acrylic resin were produced and divided according to PTBAEMA concentration: 0 (control), 10 and 25%. The specimens were inoculated (10(7) CFU/mL) and incubated at 37ºC for 48 h. After incubation, the wells were washed and each specimen was sonicated for 20 min. Replicate aliquots of resultant suspensions were plated at dilutions at 37ºC for 48 h. The number of colony-forming units (CFU) was counted and expressed as log (CFU+1)/mL and analyzed statistically with α=.05. RESULTS: The results showed that 25% PTBAEMA completely inhibited S. aureus and S. mutans biofilm formation. A significant reduction of log (CFU+1)/mL in count of S. aureus (control: 7.9±0.8A; 10%: 3.8±3.3B) and S. mutans (control: 7.5±0.7A; 10%: 5.1±2.7B) was observed for the group containing 10% PTBAEMA (Mann-Whitney,

Modelos didáticos como estratégia investigativa e colaborativa para o ensino de Botânica

A desmotivação dos alunos é frequentemente tida pelos professores de Biologia como causa do insucesso escolar. Para superar tal dificuldade, um projeto de pesquisa foi realizado, liderado pelos estudantes de uma escola pública de ensino médio no município de Vitória-ES. Em atividade investigativa acerca das principais dificuldades na aprendizagem de biologia, os conceitos de Botânica mostraram-se prioritários. Modelos didáticos para aulas de botânica foram elaborados pelos alunos de forma coletiva e colaborativa, e utilizados em aulas mais tarde, com o objetivo de tornar as aulas mais interessantes. Uma avaliação dos modelos foi realizada pelos professores de biologia e pelos alunos. A utilização dos modelos didáticos e a liderança dos alunos autores do projeto, mostram-se importantes estratégias motivadora, tendo tornado as aulas mais participativas, as relações mais horizontais entre alunos e professores e, assim, facilitou a aprendizagem de conceitos relacionados à botânica entre muitas outras habilidades

A nanoemulsion of Rosmarinus officinalis L. essential oil with allelopathic effect against Lactuca sativa L. seeds / Uma nanoemulsão a partir do óleo essencial de Rosmarinus officinalis L com efeito alelopático em sementes de Lactuca sativa L

Plant's essential oils have a wide range of allelopathic effects with potential uses as bioherbicides. In addition, the application of oils through nanoemulsions represents a promising alternative for agriculture, as it offers better performance and lowers toxic waste generation. Therefore, this study aimed to evaluate the chemical constitution of Rosmarinus officinalis (rosemary) essential oil, as well as its effects on germination, initial growth, Catalase (CAT), Peroxidase (POX), and Superoxide-Dismutase (SOD) enzymes activity of Lactuca sativa seeds. Nanoemulsions were produced at 5% concentration and then diluted with distilled water to 5.0, 7.0, and 10.0 mg/mL. We compared data obtained using variance (ANOVA) analysis, followed by Tukey's test at 5% probability. Rosemary oil showed a wide variety of terpenoid compounds, mainly the 1,8-cineol monoterpene, which accounted for 46% of the sample. The oil showed a dose-dependent negative allelopathic influence on all variables analyzed, causing a drop in germination percentage (%G), germination speed index (GVI), mean germination time (MTG), and leaf and root length. There was no change in CAT and SOD activity. The POX activity showed a reduction starting at the concentration of 7.0 mg/mL. The results showed allelopathic effects of rosemary oil, with potential use as a natural bioherbicide

Influence of the height of the mandibular ridge on the masticatory function during the functional adaptation with new complete dentures

The period of functional adaptation to a new conventional complete denture embraces many transitory issues, and this period is directly related to the rehabilitation success. Objective: This clinical trial evaluated the influence of the height of mandibular ridge on the masticatory function of complete denture (CD) wearers during the adaptation period. Methodology: A total of 28 individuals wearing new CDs (NR, n=14, normal mandibular ridges, 64±12.5 years, 9 female; RR, n=14, resorbed mandibular ridges, 69±6.8 years, 9 female) were assessed at 24 hours, 30 days, three months and six months after the insertion of the CDs for masticatory performance (MP, sieves method), satisfaction with CDs (questionnaire) and maximum occlusal bite force (MOBF) (gnatodynamometer). The classification of the mandibular ridges followed the Kapur index. Data of MP and MOBF were analyzed by two-way ANOVA and satisfaction with CDs was analyzed by Generalized Estimating Equations (GEE), α=.05. Results: Participants with NR presented better masticatory performance (p=.000 - NR 30.25±9.93%, RR 12.41±7.17%), general satisfaction (p=.047), retention of mandibular denture (p=.001), chewing ability (p=.037), and comfort of wearing a mandibular denture (p=.000). Regardless of the mandibular ridge, MP (p=.000) was higher at three (21.26±12.07%) and six months (24.25±12.26%) in comparison to 24 hours (18.09±10.89%), the MOBF (p=.000) was higher at three months (78.50±6.49 N) compared to 24 hours (57.34±5.55 N) and 30 days (62.72±5.97 N), and the comfort of wearing a mandibular denture (p=.002) at three months (1.61 ± 0.07) was greater than 24 hours (1.29±0.10) and 30 days (1.36±10). Conclusions: The study suggests that the participants with NR have higher MP and satisfaction with their CD, regardless of the follow-up period after the insertion of the new CD. After subjects received the CD, a period of 3 months was necessary for achieving better achievement MOBF, MP, and self-perceived comfort with the mandibular denture, regardless of the height of the mandibular ridge

Avanço tecnológico da cirúrgia refrativa nos distúrbios refracionais

As falhas refrativas da visão são decorrentes de alterações no tamanho do olho ou no formato da córnea ou cristalino, assim os raios de luz não conseguem ser focados na retina, levando a uma alteração da imagem. E para enxergar nitidamente o ser humano depende da refração, e para corrigir as imperfeições que não deixa esse fenômeno acontecer usa-se as cirurgias refrativas, que são um dos principais avanços tecnológicos desejados pela maioria da população que possui necessidade de usar óculos ou lentes de contato, o objetivo principal do estudo é abordar os benefícios das cirurgias refrativas para melhorar a qualidade de vida dos pacientes acometidos por distúrbios refrativos diversos. Assim realizou-se uma revisão bibliográfica nas bases de dados Pubmed, Scielo e Google acadêmico. Foram empregados trabalhos dos últimos 5 anos, e selecionados 17 referências relacionados ao tema. Sendo assim, temos como conclusão que Lasik e Prk são as mais conhecidas técnicas para corrigir os erros refrativos de miopia, hipermetropia, astigmatismo e presbiopia, apesar de serem consideradas seguras podem gerar alguma complicação no pós-operatório que deve ser comunicada previamente a todos os pacientes. Portanto, é imprescindível analisar cada caso clínico de acordo com suas peculiaridades no pré-operatório e com a atuação da equipe multidisciplinar, realizar uma investigação oftalmológica específica sobre esse assunto para direcionar mais benefícios para os indivíduos

Subclavian Access for Transcatheter CoreValve® Aortic Prosthesis Implantation: Data from the Brazilian Registry

ABSTRACTBackgroundTransfemoral access is the preferred approach for transcatheter aortic valve implantation. However, some situations, such as the presence of peripheral vascular disease, preclude the use of such access. In these cases, subclavian access is an alternative approach for this procedure. This study aimed at evaluating the Brazilian experience using the subclavian approach for transcatheter CoreValve® prosthesis implantation.MethodsAortic valve area<1cm2, aortic valve ring≥20mm and≤27mm (26mm and 29mm CoreValve®), ascending aorta≤43mm and subclavian artery with a diameter≥6mm, without significant obstructive lesions, marked tortuosity and excess calcification were requisites for the procedure. The access through the subclavian artery was obtained by surgical dissection and, under direct vision, a subclavian artery puncture was performed. Once artery access was obtained, the standard technique was used.ResultsBetween January 2008 and April 2012, 8 patients with peripheral vascular disease underwent CoreValve® prosthesis implantation through the subclavian artery in 4 institutions. The procedure was successful in all cases with reduction of the mean transvalvular pressure gradient from 46.4±17.5mmHg to 9.3±3.6mmHg (P=0.0018) and improvement of symptoms. At 30 days and after 275±231 days of follow-up, 87.5% and 62.5% of the patients, respectively, were free from major adverse events (death, myocardial infarction, stroke and urgent cardiac suregery).ConclusionsIn the Brazilian experience, the subclavian access was a safe and effective alternative for transcatheter CoreValve® implantation

Piper nigrum allelopathy on the germination and initial growth of Lactuca sativa and Panicum maximum

The increased demand for food promotes greater use of agricultural inputs and pesticides used in pest control. Many of these products have negative environmental and public health impacts. Thus, there is a need for alternative products, with allelopathic capacity that reduce such impacts. The objective of this work was to verify the allelopathic potential of Piper nigrum L. extracts on the germination and initial growth of Panicum maximum Jacq. and Lactuca sativa seeds. The experimental design used was completely randomized, using two plant organs (leaf and seed), three extractors (hexane, ethyl acetate and methanol) and five concentrations (0 mg/L, 200 mg/L, 400 mg/L, 600 mg/L and 800 mg/L). It was verified the influence of these extracts on germination speed index (IVG), germination percentage (G), mean germination time (TMG), allelopathy index (IA), shoot growth (CPA) and in the root growth (CR) of seeds and seedlings. The leaf and seed extracts, at all concentrations and extractors tested, did not show phytotoxicity or had a very small inhibitory effect on germination and initial growth of Lactuca sativa. For Panicum maximum, ethyl acetate and methanol seed extracts, at the highest concentrations tested, had the most significant negative effects on the weed. Thus, it was possible to infer that extracts from Piper nigrum seeds have potential use in the control of the weed Panicum maximum.The increased demand for food promotes greater use of agricultural inputs and pesticides used in pest control. Many of these products have negative environmental and public health impacts. Thus, there is a need for alternative products, with allelopathic capacity that reduce such impacts. The objective of this work was to verify the allelopathic potential of Piper nigrum L. extracts on the germination and initial growth of Panicum maximum Jacq. and Lactuca sativa seeds. The experimental design used was completely randomized, using two plant organs (leaf and seed), three extractors (hexane, ethyl acetate and methanol) and five concentrations (0 mg/L, 200 mg/L, 400 mg/L, 600 mg/L and 800 mg/L). It was verified the influence of these extracts on germination speed index (IVG), germination percentage (G), mean germination time (TMG), allelopathy index (IA), shoot growth (CPA) and in the root growth (CR) of seeds and seedlings. The leaf and seed extracts, at all concentrations and extractors tested, did not show phytotoxicity or had a very small inhibitory effect on germination and initial growth of Lactuca sativa. For Panicum maximum, ethyl acetate and methanol seed extracts, at the highest concentrations tested, had the most significant negative effects on the weed. Thus, it was possible to infer that extracts from Piper nigrum seeds have potential use in the control of the weed Panicum maximum

POLAR registry (Promus eluting stent registry in Latin America ):1 year follow-up results

BACKGROUND: Drug-eluting stents have been used since 2002 in different patient populations aiming to achieve high success rates with low clinical and angiographic restenosis rates. With the late thrombosis adverse events associated to the first generation sirolimus and paclitaxel-eluting stents, second-generation everolimus and zotarolimus-eluting stents has been recently developed. METHODS: The POLAR registry is a prospective, non-randomized, multicenter study, which included 988 patients, totaling 1,362 lesions treated with the everolimus-eluting stent Promus®. In order to represent the clinical practice, almost all subtypes of patients and lesions were included in this registry. Clinical follow-up was planned to be performed 1, 6, 12 and 24 months after the procedure. RESULTS: Most patients were male (69.8%), with mean age of 64.9 ± 9.4 years, 35.2% were diabetics and 55% had been treated for acute coronary syndrome. Vessel diameter was 2.95 ± 0.43 mm and lesion extension was 20.5 ± 5.6 mm. A total of 1.14 ± 0.38 stent/patient were implanted and the procedural success rate was 96.6%. Major adverse cardiac events occurred in 4.5% of patients, and stent thrombosis was observed in 5 patients (0.5%) after a clinical follow-up of 12 months. CONCLUSIONS: The present registry suggests that everolimus-eluting stents are safe and effective in daily clinical practice patients, with a low rate of major adverse cardiac events at the end of the first year of follow-up.INTRODUÇÃO: Desde 2002, os stents farmacológicos são utilizados em diversas populações de pacientes objetivando alcançar elevados índices de sucesso, com baixas taxas de reestenose angiográfica e clínica. Com os resultados adversos em relação à trombose tardia associados aos stents farmacológicos de primeira geração eluidores de sirolimus e paclitaxel, surgiram recentemente os stents farmacológicos de segunda geração eluidores de zotarolimus e everolimus. MÉTODOS: O registro POLAR é um registro prospectivo, não-randomizado, multicêntrico, que incluiu 988 pacientes totalizando 1.362 lesões tratadas com o stent Promus®. Objetivando representar a prática clínica, praticamente todos os subtipos de pacientes e lesões foram incluídos neste registro. O seguimento clínico foi planejado para ser realizado 1 mês, 6 meses, 12 meses e 24 meses após o procedimento. RESULTADOS: A maioria dos pacientes era do sexo masculino (69,8%), com média de idade de 64,9 ± 9,4 anos, 35,2% eram diabéticos e 55% tinham sido tratados na vigência de síndrome coronária aguda. O diâmetro do vaso foi de 2,95 ± 0,43 mm e a extensão da lesão, de 20,5 ± 5,6 mm. Foi implantado 1,14 ± 0,38 stent/paciente e o sucesso do procedimento foi alcançado em 96,6% dos casos. Eventos cardíacos adversos maiores ocorreram em 4,1% dos pacientes, e trombose de stent esteve presente em 5 pacientes (0,5%) após o seguimento clínico de 12 meses. CONCLUSÕES: O presente registro sugere que os stents farmacológicos eluidores de everolimus são seguros e eficazes em pacientes da prática clínica diária, com baixas taxas de eventos cardíacos adversos maiores ao término do primeiro ano de seguimento.Universidade Federal de São Paulo (UNIFESP)Instituto Dante Pazzanese de CardiologiaStatus CorHospital Israelita Albert EinsteinENCORE Hospital Lúcio RebeloReal e Benemérita Associação Portuguesa de BeneficênciaHospital Cardiológico CostantiniHemodinâmica Meridional Intercath MeridionalInstituto de Cardiologia do Espírito SantoFundação Universitária de Cardiologia Instituto de Cardiologia do Rio Grande do SulUNIFESPSciEL

Diagnóstico de comunidade de uma área geograficamente delimitada dentro do distrito sanitário 8 de Porto Alegre : achados referentes a população feminina com mais de 12 anos

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