Factores asociados al parto prematuro en madres del servicio Gineco-Obstetricia del Hospital San Juan de Lurigancho, Lima 2022

Mediante este estudio realizado en el hospital San Juan de Lurigancho se determinó que, los problemas de salud de las gestantes que se atienden en el hospital están relacionados con los comportamientos y estilos de vida que llevan antes y durante el embarazo. Por eso, la investigación tuvo como objetivo determinar los factores más prevalentes al parto prematuro en madres del servicio Gineco -Obstetricia del Hospital San Juan de Lurigancho, Lima 2022. El estudio fue de enfoque cuantitativo, de nivel descriptivo, diseño no experimental. La muestra, estuvo conformada por 102 madres que han tenido parto pretérmino atendidas en el servicio Gineco-Obstetra del hospital de san Juan de Lurigancho, en el mes de enero hasta abril del año 2022. La técnica, utilizada fue el análisis documental y el instrumento utilizado fue ficha de registro de que fue validado por cinco expertos. Obteniendo como resultados que el 29,50% de las madres tiene una prevalencia del factor materno en el parto prematuro, el 12% de las madres tiene una prevalencia del factor fetal en el parto prematuro y el 10% de las madres tiene una prevalencia del factor ovulares en el parto prematuro. Además, en la dimensión factores maternos el 70% de los partos prematuros en el hospital San Juan de Lurigancho del servicio Gineco – Obstetricia están ausentes y el 30% de los factores están presentes. En la segunda dimensión, se observó que el 88 % de los factores fetales al parto prematuro en madres del servicio Gineco -Obstetricia del Hospital San Juan de Lurigancho están ausentes, mientras que el 12 % están presentes. En la dimensión factores ovulares el 90% del parto prematuro en madres del servicio Gineco -Obstetricia del Hospital San Juan de Lurigancho están Ausentes, mientras que el 10 % están presentes. Concluyendo, que el factor materno es el más prevalente para tener un parto prematuro y el menos prevalente es el factor ovular coincidiendo así con las otras investigaciones respecto a este tema

Marketing digital y su influencia en la fidelización de los clientes de la Concesionaria H&S S.A.C., Chimbote 2019

La presente investigación tuvo como objetivo general determinar la influencia del marketing digital en la fidelización de los clientes de la Concesionaria H&S S.A.C., Chimbote 2019. En donde se definió al marketing digital en como lograr que el cliente ejecute una acción favorable para la empresa, que ésta haya planeado, a partir de las estrategias de mercado que se ejecutan en el mundo digital y son percibidas por el cliente cuando visita la página web o páginas en redes sociales de la empresa (Selman, 2017). Asimismo, la fidelización del cliente es el comportamiento que el cliente adopta en relación a una marca en específico a lo largo del tiempo (Salvador, 2011). El método de la investigación tuvo un enfoque cuantitativo, con diseño no experimental y corte transversal con una población que estuvo conformada por 1,000 clientes y se diseñaron dos cuestionarios que fueron aplicados una muestra de 278 clientes. El coeficiente de correlación Rho de Spearman dio como resultado 0.965, lo que indica el Marketing Digital influye en la Fidelización de los clientes de manera positiva y alta. Además, al ser el nivel de significancia (0.00) menor a 0,05, se rechazó la hipótesis nula (Ho), y, por consiguiente, se aceptó la hipótesis alternativa Hi: El marketing digital sí influye en la fidelización de los clientes de la Concesionaria H&S S.A.C., Chimbote 2019. Además, que el 39% de los clientes manifestó que el marketing digital de la empresa fue malo. También, que se determinó el nivel fidelización de los clientes, resultando ser baja según el 47%

Green entrepreneurship intention among high school students: a teachers’ view

There is a gap in the view of teachers about green entrepreneurship intention in high school students. The objective of the current article is to evaluate the factors that explain from private high schools in Peru the intention of green entrepreneurship from the view of teachers. It was carried out with a sample size of 163 teachers from fifth high schools. The research model was evaluated by partial least square structural equation modeling (PLS-SEM) which is a technique for multivariate analysis. Extracurricular support and country support showed an effect on entrepreneurial self-efficacy. Also, entrepreneurial self-efficacy had an effect on green entrepreneurship intention. Finally, self-efficacy was a mediator between extracurricular support, country support, and green entrepreneurial intention. These outcomes can be used by the Ministry of Education and high school planners to use the variables that show more effect and include strategies to increase the green entrepreneurial intention among school students as the creation of annual programs of practical training and entrepreneurship accelerators to develop skills among the students. Also, it can be relevant that the professors can receive specific training in green entrepreneurship because they can guide their students during the daily classes and extracurricular activities as well

Simulation of High-Performance Memory Allocators

This study presents a single-core and a multi-core processor architecture for health monitoring systems where slow biosignal events and highly parallel computations exist. The single-core architecture is composed of a processing core (PC), an instruction memory (IM) and a data memory (DM), while the multi-core architecture consists of PCs, individual IMs for each core, a shared DM and an interconnection crossbar between the cores and the DM. These architectures are compared with respect to power vs. performance trade-offs for a multi-lead electrocardiogram signal conditioning application exploiting near threshold computing. The results show that the multi-core solution consumes 66%less power for high computation requirements (50.1 MOps/s), whereas 10.4% more power for low computation needs (681 kOps/s)

Simulation of High-Performance Memory Allocators

Current general-purpose memory allocators do not provide sufficient speed or flexibility for modern highperformance applications. To optimize metrics like performance, memory usage and energy consumption, software engineers often write custom allocators from scratch, which is a difficult and error-prone process. In this paper, we present a flexible and efficient simulator to study Dynamic Memory Managers (DMMs), a composition of one or more memory allocators. This novel approach allows programmers to simulate custom and general DMMs, which can be composed without incurring any additional runtime overhead or additional programming cost. We show that this infrastructure simplifies DMM construction, mainly because the target application does not need to be compiled every time a new DMM must be evaluated. Within a search procedure, the system designer can choose the "best" allocator by simulation for a particular target application. In our evaluation, we show that our scheme will deliver better performance, less memory usage and less energy consumption than single memory allocators

Influence of Neospora caninum intra-specific variability in the outcome of infection in a pregnant BALB/c mouse model

Previous assays in pregnant animals have demonstrated the effect of different host factors and timing of infection on the outcome of neosporosis during pregnancy. However, the influence of Neospora caninum isolate itself has been poorly investigated. Here, we compared the effects on clinical outcome and vertical transmission observed in a pregnant mouse model following infection with 10 different N. caninum isolates. The isolates in our study included the Nc-Liv isolate and nine N. caninum isolates obtained from calves. Female BALB/c mice were inoculated with 2 × 106 tachyzoites at day 7 of pregnancy. Morbidity and mortality, in both dams and offspring during the course of infection, and transmission to progeny at day 30 postpartum were evaluated. The serum IgG1 and IgG2a production in dams were also examined. All dams showed elevated IgG1 and IgG2a responses, confirming N. caninum infection, although signs of disease were only exhibited in dams infected with 4 of the 10 isolates (Nc-Spain 4H, Nc-Spain 5H, Nc-Spain 7 and Nc-Liv). In neonates, clinical signs were observed in all N. caninum-infected groups, and neonatal mortality rates varied from greater than 95% with the isolates mentioned above to less than 32.5% with the other isolates. Vertical transmission rates, as assessed by parasite PCR-detection in neonate brains, also varied from 50% to 100% according to the isolate implicated. These results confirm the wide pathogenic and transmission variability of N. caninum. The intra-specific variability observed herein could help us explain the differences in the outcome of the infection in the natural host

Unraveling the antiviral activity of plitidepsin against SARS-CoV-2 by subcellular and morphological analysis

The pandemic caused by the new coronavirus SARS-CoV-2 has made evident the need for broad-spectrum, efficient antiviral treatments to combat emerging and re-emerging viruses. Plitidepsin is an antitumor agent of marine origin that has also shown a potent pre-clinical efficacy against SARS-CoV-2. Plitidepsin targets the host protein eEF1A (eukaryotic translation elongation factor 1 alpha) and affects viral infection at an early, post-entry step. Because electron microscopy is a valuable tool to study virus-cell interactions and the mechanism of action of antiviral drugs, in this work we have used transmission electron microscopy (TEM) to evaluate the effects of plitidepsin in SARS-CoV-2 infection in cultured Vero E6 cells 24 and 48h post-infection. In the absence of plitidepsin, TEM morphological analysis showed double-membrane vesicles (DMVs), organelles that support coronavirus genome replication, single-membrane vesicles with viral particles, large vacuoles with groups of viruses and numerous extracellular virions attached to the plasma membrane. When treated with plitidepsin, no viral structures were found in SARS-CoV-2-infected Vero E6 cells. Immunogold detection of SARS-CoV-2 nucleocapsid (N) protein and double-stranded RNA (dsRNA) provided clear signals in cells infected in the absence of plitidepsin, but complete absence in cells infected and treated with plitidepsin. The present study shows that plitidepsin blocks the biogenesis of viral replication organelles and the morphogenesis of virus progeny. Electron microscopy morphological analysis coupled to immunogold labeling of SARS-CoV-2 products offers a unique approach to understand how antivirals such as plitidepsin work.info:eu-repo/semantics/publishedVersio

β-Cyclodextrins as affordable antivirals to treat coronavirus infection

The SARS-CoV-2 pandemic made evident that there are only a few drugs against coronavirus. Here we aimed to identify a cost-effective antiviral with broad spectrum activity and high safety profile. Starting from a list of 116 drug candidates, we used molecular modelling tools to rank the 44 most promising inhibitors. Next, we tested their efficacy as antivirals against α and β coronaviruses, such as the HCoV-229E and SARS-CoV-2 variants. Four drugs, OSW-1, U18666A, hydroxypropyl-β-cyclodextrin (HβCD) and phytol, showed in vitro antiviral activity against HCoV-229E and SARS-CoV-2. The mechanism of action of these compounds was studied by transmission electron microscopy and by fusion assays measuring SARS-CoV-2 pseudoviral entry into target cells. Entry was inhibited by HβCD and U18666A, yet only HβCD inhibited SARS-CoV-2 replication in the pulmonary Calu-3 cells. Compared to the other cyclodextrins, β-cyclodextrins were the most potent inhibitors, which interfered with viral fusion via cholesterol depletion. β-cyclodextrins also prevented infection in a human nasal epithelium model ex vivo and had a prophylactic effect in the nasal epithelium of hamsters in vivo. All accumulated data point to β-cyclodextrins as promising broad-spectrum antivirals against different SARS-CoV-2 variants and distant alphacoronaviruses. Given the wide use of β-cyclodextrins for drug encapsulation and their high safety profile in humans, our results support their clinical testing as prophylactic antivirals

β-Cyclodextrins as affordable antivirals to treat coronavirus infection

The SARS-CoV-2 pandemic made evident that there are only a few drugs against coronavirus. Here we aimed to identify a cost-effective antiviral with broad spectrum activity and high safety profile. Starting from a list of 116 drug candidates, we used molecular modelling tools to rank the 44 most promising inhibitors. Next, we tested their efficacy as antivirals against α and β coronaviruses, such as the HCoV-229E and SARS-CoV-2 variants. Four drugs, OSW-1, U18666A, hydroxypropyl-β-cyclodextrin (HβCD) and phytol, showed in vitro antiviral activity against HCoV-229E and SARS-CoV-2. The mechanism of action of these compounds was studied by transmission electron microscopy and by fusion assays measuring SARS-CoV-2 pseudoviral entry into target cells. Entry was inhibited by HβCD and U18666A, yet only HβCD inhibited SARS-CoV-2 replication in the pulmonary Calu-3 cells. Compared to the other cyclodextrins, β-cyclodextrins were the most potent inhibitors, which interfered with viral fusion via cholesterol depletion. β-cyclodextrins also prevented infection in a human nasal epithelium model ex vivo and had a prophylactic effect in the nasal epithelium of hamsters in vivo. All accumulated data point to β-cyclodextrins as promising broad-spectrum antivirals against different SARS-CoV-2 variants and distant alphacoronaviruses. Given the wide use of β-cyclodextrins for drug encapsulation and their high safety profile in humans, our results support their clinical testing as prophylactic antivirals.This work has been funded by grant RTI2018-094445-B100 (MCIU/AEI/FEDER, UE) from the Ministry of Science and Innovation of Spain (C.R.), by Palex Medical S.A., Sika S.A.U. and 7 more companies, and by Ms. Raquel Casaus Alvarez, Mr. Miguel Pardo Gil, Mr. Jacques Noguès and a total of 2916 citizens through the Precipita crowdfunding platform of Fecyt (Fundación Española para la Ciencia y la Tecnología). NI-U is supported by the Spanish Ministry of Science and Innovation (grant PID2020-117145RB-I00), EU HORIZON-HLTH-2021-CORONA-01 (grant 101046118) and by institutional funding of Grifols, Pharma Mar, HIPRA, Amassence and Palobiofarma. This work used the computational resources of the Centro de Supercomputación de Galicia (CESGA) supported by the Partnership for Advanced Computing in Europe (PRACE) COVID-19 Fast Track Call for Proposals – Allocation Decision – Proposal COVID19-85.info:eu-repo/semantics/publishedVersio

Infected Dendritic Cells Facilitate Systemic Dissemination and Transplacental Passage of the Obligate Intracellular Parasite Neospora caninum in Mice

The obligate intracellular parasite Neospora caninum disseminates across the placenta and the blood-brain barrier, to reach sites where it causes severe pathology or establishes chronic persistent infections. The mechanisms used by N. caninum to breach restrictive biological barriers remain elusive. To examine the cellular basis of these processes, migration of different N. caninum isolates (Nc-1, Nc-Liverpool, Nc-SweB1 and the Spanish isolates: Nc-Spain 3H, Nc-Spain 4H, Nc-Spain 6, Nc-Spain 7 and Nc-Spain 9) was studied in an in vitro model based on a placental trophoblast-derived BeWo cell line. Here, we describe that infection of dendritic cells (DC) by N. caninum tachyzoites potentiated translocation of parasites across polarized cellular monolayers. In addition, powered by the parasite's own gliding motility, extracellular N. caninum tachyzoites were able to transmigrate across cellular monolayers. Altogether, the presented data provides evidence of two putative complementary pathways utilized by N. caninum, in an isolate-specific fashion, for passage of restrictive cellular barriers. Interestingly, adoptive transfer of tachyzoite-infected DC in mice resulted in increased parasitic loads in various organs, e.g. the central nervous system, compared to infections with free parasites. Inoculation of pregnant mice with infected DC resulted in an accentuated vertical transmission to the offspring with increased parasitic loads and neonatal mortality. These findings reveal that N. caninum exploits the natural cell trafficking pathways in the host to cross cellular barriers and disseminate to deep tissues. The findings are indicative of conserved dissemination strategies among coccidian apicomplexan parasites