3,589 research outputs found

    Electroactivación de oocitos de pez cebra (Danio rerio)

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    [ES] El mejor tratamiento de electroactivación (B3) para oocitos de pez cebra supuso un 43% de activación frente al 18% cuando el estímulo es solo el contacto con agua[EN] In zebrafish, initial egg activation by water after being discharged from the ovarian stroma is followed by normal cleavages only in inseminated eggs. When sperm (genetically active or not) is not used as activating agent, reproductive techniques (as either nuclear transplant or intracytoplasmic sperm injection are ineff icient. In this work, three experiments of egg activation by electric stimuli were performed: In the first, six activation treatments were compared (Voltage × Pulses: 2.76 × 1; 2.76 × 2; 2.76 × 3 and 5.40 × 1; 5.40 × 2; 5.40 × 3). The group 5.40 × 3 showed the best results (32% activated). In the second experiment, an electrical treatment of 20 min was carried out. It consisted in a sequence of three equal electrical stimuli every 10 min (of 1 or 3 consecutive direct current square pulses for 20 ¿s each and applied at two voltage levels, 2.76 V or 5.4 V). It was observed that the number of pulses negatively affected the rates of damaged and lysed eggs. Moreover, only the 20 min treatment with the combination of 3 consecutive pulses at 2.76 V showed significant differences with their respective control group (43% vs 18% activated eggs, p < 0.05). In the third experiment, negative effects of egg ageing were observed. In conclusion, the best activation treatment for intact (non manipulated) zebrafish eggs concerns sequence B3. The electro-activation stimulus proposed here would be the only one available once the egg has already been activated by water.Cardona Costa, J.; Perez-Camps, M.; García Ximenez, F. (2011). Electroactivation of zebrafish (danio rerio) eggs. Spanish Journal of Agricultural Research. 9(1):59-65. http://hdl.handle.net/10251/99491S59659

    Mielopatía estenótica vertebral cervical (MEVC) en un caballo criollo colombiano.

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    We present the case of a Colombian criollo horse, fine gauge, color moro, entire male and 17 years old, who was diagnosed with cervical vertebral stenotic myelopathy (CVSM), from the town of Chimá (Cordoba, Colombia). At neurological examination detected symmetric ataxia worsened to cover the eyes, signs of incoordination, stumbling to the test latero drive, weakness and loss of proprioception primarily detected in the hindquarters with proprioception test, test hoping or hemi-jump was positive, radiology underwent cervical latero-lateral projection, detecting double medullary canal stenosis at the cervical vertebrae C2- C3 and C3-C4. These findings are mainly due to cervical vertebral stenotic myelopathy (CVSM). This condition is also known as equine Woobler´s syndrome, which is a developmental disease characterized by cervical spinal canal stenosis resulting in intermittent or continuous compression of the spinal cord.   Se expone el caso de un caballo criollo Colombiano, de trocha fina, color moro, macho entero y 17 años de edad, el cual fue diagnosticado con Mielopatía Estenótica Vertebral Cervical (MEVC), procedente del municipio de Chimá (Córdoba, Colombia). Al examen neurológico se le detectó ataxia simétrica que empeoró al tapar los ojos, signos de incoordinación con tropiezos a la prueba de latero pulsión, debilidad y pérdida de la propiocepción principalmente en los miembros posteriores detectada con la prueba de propiocepción consciente, la prueba de Hoping o hemisalto fue positiva, razón por la cual se le realizó radiología cervical en proyección latero-lateral, detectando doble estenosis de canal medular a nivel de las vértebras cervicales C2-C3 y C3-C4. Estos hallazgos obedecen principalmente a Mielopatía Estenótica Vertebral Cervical (MEVC). Esta alteración es también conocida en equinos como Síndrome de Wooblers, la cual es una enfermedad del desarrollo caracterizada por estenosis del canal vertebral cervical resultando en compresión intermitente o continua de la médula espinal

    Holistic Disaster Risk Evaluation for the Urban Risk Management Plan of Manizales, Colombia

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    Disaster risk depends on both the physical vulnerability and a wide range of social, economic, and environmental aspects of a society. For a better risk understanding, a holistic or integrated perspective was considered when risk was assessed for the city of Manizales, Colombia. This assessment accounts not only for the expected physical damage and loss, but also for the socioeconomic vulnerability factors that favor second order effects in a disaster. This comprehensive approach allows the identification of different aspects related to physical vulnerability, social fragility, and lack of resilience that can be improved, thus enhancing integrated disaster risk management actions. The outcomes of this comprehensive assessment are currently being used as input to update the disaster risk management plan of Manizales

    CFD analysis of the needle tip angle in Pelton injector on jet quality for the power generation

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    Fossil fuels are energy sources that supply a large part of the world's energy generation. However, they produce greenhouse gases such as carbon dioxide (CO2), nitrogen oxide (NOx) and particulates that increase global warming. For this reason, other forms of renewable energy such as hydropower have begun to be implemented through turbomachinery such as Pelton turbines, which significantly reduce these emissions since they are highly efficient turbines based on the use of natural resources (water). Pelton turbines are based mainly on three components for their operation, which are the Pelton injector, the bucket and the wheel. The injector is an important component in the energy transformation of Pelton turbines. Although to analyze its behavior, it is possible to use fluid dynamics (CFD) software to predict the trajectory of the flow through a solid or free surface. The objective of this work is to analyze by means of computational fluid dynamics (CFD) the incidence of the length and the needle tip angle of a Pelton turbine injector on the generated power. For this, an ANSYS&nbsp;2020R2 computational fluid analysis software was used to study how the variation of the injector needle tip angle influences through the volume of fluid (VOF) method, starting from the generation of a commercial model with a tip angle of 60° and two (2) geometries of 55° and 75° respectively. Numerical results show a better performance for the 75° angle of 96&nbsp;% and lower for the 55° and 60° with 94.1&nbsp;% and 95.5&nbsp;% respectively, whereby steeper angles achieve higher performances. In summary, the present study pretends to increase the power generation, in the face of phenomena occurred in the energy transfer. Although the performance of the injector in each angle configuration must be tested in practic

    Introducción a la tecnología de membranas para la purificación de biogas y algunos desarrollos recientes

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    The remotion of CO2 from CO2/CH4 mixes to increasing energy content in natural gas or biogas and to prevent corrosion problems, has driven the development of CO2 separation process through membranes.&nbsp; The attractive features offered by this technology include high energy efficiency, reduced cost and highly flexible performance. This review provides an overview of the reported paper from 2010 to 2020 different types of membranes available: polymeric, inorganic and mixed matrix for CO2/CH4 separation process, experimental conditions and primary determinants of separation performance and efficiency (permeability of CO2 and CO2/CH4 selectivity). &nbsp;This work would open up a new perspective of each membrane to facilitate a better appreciation of their role in the improvement of overall process performance.La remoción de CO2 en mezclas de CO2/CH4 para aumentar el contenido de energía en gas natural o biogás y prevenir problemas de corrosión, ha impulsado el desarrollo del proceso de separación de CO2 utilizando membranas. &nbsp;Las características más relevantes que ofrece la tecnología basada en membranas incluyen la alta eficiencia energética, el costo reducido y el rendimiento altamente flexible. Esta revisión &nbsp;proporciona una descripción de los trabajos reportados desde 2010 hasta 2020 sobre los diferentes tipos de membranas disponibles: poliméricas, inorgánicas y de matriz mixta para el proceso de separación de CO2/CH4;&nbsp; se reportan las condiciones experimentales y los determinantes primarios del rendimiento y la eficiencia de la separación (permeabilidad de CO2 y selectividad CO2/CH4). &nbsp;Este trabajo ofrece una nueva perspectiva de cada membrana para facilitar una mejor apreciación de su papel en la mejora del rendimiento general del proceso &nbsp; The remotion of CO2 from CO2/CH4 mixes to increasing energy content in natural gas or biogas and to prevent corrosion problems, has driven the development of CO2 separation process through membranes.&nbsp; The attractive features offered by this technology include high energy efficiency, reduced cost and highly flexible performance. This review provides an overview of the reported paper from 2010 to 2020 different types of membranes available: polymeric, inorganic and mixed matrix for CO2/CH4 separation process, experimental conditions and primary determinants of separation performance and efficiency (permeability of CO2 and CO2/CH4 selectivity). &nbsp;This work would open up a new perspective of each membrane to facilitate a better appreciation of their role in the improvement of overall process performance

    Holistic disaster risk evaluation for the urban risk management plan of Manizales, Colombia

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    Disaster risk depends on both the physical vulnerability and a wide range of social, economic, and environmental aspects of a society. For a better risk understanding, a holistic or integrated perspective was considered when risk was assessed for the city of Manizales, Colombia. This assessment accounts not only for the expected physical damage and loss, but also for the socioeconomic vulnerability factors that favor secondorder effects in a disaster. This comprehensive approach allows the identification of different aspects related to physical vulnerability, social fragility, and lack of resilience that can be improved, thus enhancing integrated disaster risk management actions. The outcomes of this comprehensive assessment are currently being used as input to update the disaster risk management plan of Manizales.Peer ReviewedPostprint (published version

    El conocimiento profesional del profesorado de ciencias sobre la educación ambiental: Primera fase.

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    Este artículo presenta los elementos correspondientes a la fase de fundamentación y diseño metodológico del proyecto de tesis doctoral que se desarrolla en el marco del programa oficial de postgrado “Investigación en la Enseñanza y el Aprendizaje de las Ciencias Experimentales, Sociales y Matemáticas”, desde el curso 2007-2008 y que se encuentra adscrito al Departamento de Didáctica de las Ciencias y Filosofía de la Universidad de Huelva. Bajo la línea de Desarrollo Profesional, en este trabajo se caracterizan los elementos del conocimiento profesional del profesor de ciencias naturales en el campo de la educación ambiental y los factores que inciden para promover u obstaculizar el desarrollo profesional de los maestros
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