Total and Potentially Active Bacterial Communities Entrapped in a Late Glacial Through Holocene Ice Core From Scarisoara Ice Cave, Romania

Our understanding of the icy-habitat microbiome is likely limited by a lack of reliable data on microorganisms inhabiting underground ice that has accumulated inside caves. To characterize how environmental variation impacts cave ice microbial community structure, we determined the composition of total and potentially active bacterial communities along a 13,000-year-old ice core from Scarisoara cave (Romania) through 16S rRNA gene Illumina sequencing. An average of 2,546 prokaryotic gDNA operational taxonomic units (OTUs) and 585 cDNA OTUs were identified across the perennial cave ice block and analyzed in relation to the geochemical composition of ice layers. The total microbial community and the putative active fraction displayed dissimilar taxa profiles. The ice-contained microbiome was dominated by Actinobacteria with a variable representation of Proteobacteria, while the putative active microbial community was equally shared between Proteobacteria and Firmicutes. Accordingly, a major presence of Cryobacterium, Lysinomonas, Pedobacter, and Aeromicrobium phylotypes homologous to psychrotrophic and psychrophilic bacteria from various cold environments were noted in the total community, while the prevalent putative active bacteria belonged to Clostridium, Pseudomonas, Janthinobacterium, Stenotrophomonas, and Massilia genera. Variation in the microbial cell density of ice strata with the dissolved organic carbon (DOC) content and the strong correlation of DOC and silicon concentrations revealed a major impact of depositional processes on microbial abundance throughout the ice block. Post-depositional processes appeared to occur mostly during the 4,000–7,000 years BP interval. A major bacterial composition shift was observed in 4,500–5,000-year-old ice, leading to a high representation of Beta- and Deltaproteobacteria in the potentially active community in response to the increased concentrations of DOC and major chemical elements. Estimated metabolic rates suggested the presence of a viable microbial community within the cave ice block, characterized by a maintenance metabolism in most strata and growth capacity in those ice deposits with high microbial abundance and DOC content. This first survey of microbial distribution in perennial cave ice formed since the Last Glacial period revealed a complex potentially active community, highlighting major shifts in community composition associated with geochemical changes that took place during climatic events that occurred about 5,000 years ago, with putative formation of photosynthetic biofilms

STUDIES ON DIRECT CAULOGENESIS CULTURE OF MORINGA OLEIFERA, AN EXOTIC MULTIPURPOSE TREE SPECIES FOR ROMANIA

In Romania, M. oleifera Lam. species caught the attention of growers and consumers only with five years ago. Because planting material for commercial cultivation is now very low accesible, as for the culture establishment, like many other species of cultivated plants M.oleifera Lam., tehniques "in vitro" culture applications have the outstanding contributions to overcoming of the limits facing culture technology of this species. First, the attention is given to rapid multiplication of biological material genetically pure and at the same time is free from diseases and pests. The method of micropropagation by axillary branching method using apical buds as explants and nodal explants from seedlings was initiated in this study. Cultures were initiated by multiple shoots on MS medium containing variable concentrations of BA 1.0-2.5 mg/l and IBA 0.2 mg/l. Maximum shoot proliferation was achieved on medium containing BA more than 2.0 mg /l and 0.2 mg/l IBA within 3 subcultures. The proliferation of shoots was improved in the medium of the same composition after the addition of citric acid as an antioxidant in reducing the emission of polyphenols. A maximum rate of 5 copies of shoots was performed on the addition of citric acid (10 mg/l) in the environment. Leaves were excised from the M.oleifera Lam. plants propagated through "in vitro", for callus induction

Long-term land-cover/use change in a traditional farming landscape in Romania inferred from pollen data, historical maps and satellite images

Traditional farming landscapes in the temperate zone that have persisted for millennia can be exceptionally species-rich and are therefore key conservation targets. In contrast to Europe’s West, Eastern Europe harbours widespread traditional farming landscapes, but drastic socio-economic and political changes in the twentieth century are likely to have impacted these landscapes profoundly. We reconstructed long-term land-use/cover and biodiversity changes over the last 150 years in a traditional farming landscape of outstanding species diversity in Transylvania. We used the Regional Estimates of Vegetation Abundance from Large Sites model applied to a pollen record from the Transylvanian Plain and a suite of historical and satellite-based maps. We documented widespread changes in the extent and location of grassland and cropland, a loss of wood pastures as well as a gradual increase in forest extent. Land management in the socialist period (1947–1989) led to grassland expansion, but grassland diversity decreased due to intensive production. Land-use intensity has declined since the collapse of socialism in 1989, resulting in widespread cropland abandonment and conversion to grassland. However, these trends may be temporary due to both ongoing woody encroachment as well as grassland management intensification in productive areas. Remarkably, only 8% of all grasslands existed throughout the entire time period (1860–2010), highlighting the importance of land-use history when identifying target areas for conservation, given that old-growth grasslands are most valuable in terms of biodiversity. Combining datasets from different disciplines can yield important additional insights into dynamic landscape and biodiversity changes, informing conservation actions to maintain these species-rich landscapes in the longer term

How warm? How wet? Hydroclimate reconstruction of the past 7500 years in northern Carpathians, Romania

As natural and anthropogenic ecosystems are dependent on the local water availability, understanding past changes in hydroclimate represents a priority in research concerning past climate variability. Here, we used testate amoebae (TA) and chironomid analysis on a radiocarbon dated complex of small pond and peat bog sediment profiles from an ombrotrophic bog (Taut Muced, northern Carpathians, Romania) to quantitatively determine major hydrological changes and July air temperature over the last 7500 years.Wet mire surface conditions with a pH between 23 and 4.5 were inferred for the periods 4500-2700 and 1300400 cal yr BP by the occurrence of Archerella flavum, Amphitrema wrightianum and Hyalosphenia papilio. Dry phases in mire surface conditions and a pH between 2.5 and 5 were inferred for 7550-4500, 2750-1300 and 0 cal yr BP-present by the dominance of Nebela militaris, Difflugia pulex and Phryganella acropodia. The quantitative reconstruction of mean July temperature based on the chironomid communities suggests low summer temperatures for the periods 6550-5600, 4500-3150 and 1550-600 cal yr BP, while periods of slightly higher summer temperatures were observed for 5600-4500,3150-1550 and 100 cal yr BP-present. There is a generally good agreement between drier phases of the peat surface conditions with higher July temperature, suggesting that temperature may have been a controlling factor for water table fluctuation.Our quantitative reconstructions, among the first for central eastern Europe, show a relatively good agreement with other palaeohydrological studies from central eastern Europe, but contrast with others estimates from north-west Europe. Another important aspect of our study is that it provides valuable information on changes in local hydrology and the potential effect of the mean summer temperature over these changes. (C) 2017 Elsevier B.V. All rights reserved

The Interplay Between Air Temperature and Ice Mass Balance Changes in Scarisoara Ice Cave, Romania

This paper examines the short-term relations established between external and cave air temperature in Scărişoara Ice Cave (Romania) and the role they play upon ice genesis and mass balance changes. Geothermal heat and external climate are the main drivers of the cave’s air temperature, but the ice forming and ablation processes modulate its spatial and temporal characteristics. In the winter half-year, cold air inflow leads to the overcooling of the cave atmosphere and walls and ice formation; while in summer, melting of ice acts as strong thermal sink, keeping the air temperature at 0 °C. In autumn and winter, dynamic cooling of the cave atmosphere leads to ice build-up, whereas in summer, the causality is overturned, the cave air temperature being controlled by the melting ice. The existence of a net heat sink in the cave (melting ice in summer in this case), leads to the overcooling of the non-glaciated parts of the cave as well, a phenomenon that can hamper paleoclimatic reconstructions based on stable isotope studies in speleothems

Ice genesis and its long-term mass balance and dynamics in Scărişoara Ice Cave, Romania

The paleoclimatic significance of the perennial ice deposit in Scărişoara Ice Cave has been remarked on since the early 20th century, but a lack of understanding of the processes involved in the genesis, age and long-term dynamics and volume fluctuations of ice hampered all attempts to extract valuable data on past climate and vegetation changes. In this paper, we present a model of ice genesis and dynamics, based on stable isotopes, ice level monitoring (modern and archived) and radiocarbon dating of organic matter found in the ice. Ice in this cave mostly consists of layers of lake ice, produced as liquid water freezes from top to bottom in mid-autumn, and floor ice, produced as inflow water in winter freezes on top of the lake ice. This mechanism was also acting in the past, during the Medieval Warm Period and the Little Ice Age. The ice block is not stable in shape and volume, being continuously modified by ablation on top and sides, basal melting and lateral flow. Radiocarbon dating shows that the ice block is older than 1000 years, but ice flow and differential basal melting suggesting that the ice could be much older

Ice in Caves

Perennial ice in caves is a relatively uncommon occurrence, resulting from the combination of peculiar cave morphology and local climatic conditions. It exists in various forms, ranging from delicate ice crystals, various types of stalagmites and stalactites, to underground glaciers, tens of meters thick and thousands of years old. Underground glaciers are of especially great scientific importance as they hold invaluable informations on past climatic and environmental changes. In this article, we touch on various aspects related to the presence of ice in caves, highlighting the recent advances in scientific knowledge

Cryogenic Carbonates in Cave Environments: A Review

Cryogenic cave carbonate (CCC) represents a specific type of speleothem. Its precipitation proceeds at the freezing point and is triggered by freezing-induced concentration of solutes. Compared to classical speleothems (stalagmites, flowstones), CCC occurs as accumulations of loose uncemented aggregates. The grain sizes range from less than 1 μm to over 1 cm in diameter. Karst groundwater chemistry and its freezing rate upon entering the cave are responsible for highly variable grain morphology. Rapid freezing of water results in the formation of CCC powders with grain size typically below 50 μm. Slow freezing of water in caves (usually in systems where the CO2 escape is partly restricted; e.g., ice covered water pools) results in the formation of large mineral grains, with sizes from less than 1 mm to about 20 mm. The range of carbon and oxygen stable isotope compositions of CCC is larger than for a typical carbonate speleothem. Rapid freezing of water accompanied by a quick kinetic CO2 degassing results in large ranges of δ13C of the CCC powders (between –10‰ and +18‰ PDB). Slow freezing of water, with a restricted CO2 escape results in gradual increase of δ13C values (from −9‰ to +6‰ PDB; data ranges in individual caves are usually much more restricted), accompanied by a δ18O decrease of the precipitated carbonate (overall range from −10‰ to −24‰ PDB). These unusual trends of the carbonate δ18O evolution reflect incorporation of the heavier 18O isotope into the formed ice. New isotope data on CCC from three Romanian ice caves allow better understanding of the carbon and oxygen isotope fingerprint in carbonates precipitated from freezing of bulk water. CCCs are proposed as a new genetic group of speleothems