Kebijakan Parkir Kota Batam Dalam Meningkatkan Pendapatan Asli Daerah

The growth rate of motor vehicles in Batam City in recent years runs very rapidly. This levy will increase the original revenue of Batam City, But the implementation of the policy of Bylaw No.1 / 2012 on parking that has been running for three years (2012-2015) does not generate significant revenue on parking charges. The Purpose of this research is how the implementationn of parking management system based on evaluation of the policy of Bylaw No.1 / 2012 on parking Btam City. Data retrieval method is done by observation and documentation. Informant selection technique is done by purposive sampling, technical data analysis is done by data reduction, data presentation and conclusion drawing. The result of the research is that the policy of Batam City Government in increasing its achievement PAD not yet optimal, due to some obstacles such as: parking management system is not feasible, there is no public satisfaction survey index to measure the extent to which the achievement of parking policy in Batam City, Human Power that needs coaching and training. Its solution of Batan City government re-filed Ranpeda about parking with the intention of changing parking rates, changing the system of parking management and service facility improvemen

Exact protein distributions for stochastic models of gene expression using partitioning of Poisson processes

Stochasticity in gene expression gives rise to fluctuations in protein levels across a population of genetically identical cells. Such fluctuations can lead to phenotypic variation in clonal populations, hence there is considerable interest in quantifying noise in gene expression using stochastic models. However, obtaining exact analytical results for protein distributions has been an intractable task for all but the simplest models. Here, we invoke the partitioning property of Poisson processes to develop a mapping that significantly simplifies the analysis of stochastic models of gene expression. The mapping leads to exact protein distributions using results for mRNA distributions in models with promoter-based regulation. Using this approach, we derive exact analytical results for steady-state and time-dependent distributions for the basic 2-stage model of gene expression. Furthermore, we show how the mapping leads to exact protein distributions for extensions of the basic model that include the effects of post-transcriptional and post-translational regulation. The approach developed in this work is widely applicable and can contribute to a quantitative understanding of stochasticity in gene expression and its regulation.Comment: 10 pages, 5 figure

Impulse responses of three respirometry chambers with five different flow rates.

<p>None of the impulse responses are in the form of pure exponential decay.</p

Estimation of Instantaneous Gas Exchange in Flow-Through Respirometry Systems: A Modern Revision of Bartholomew's Z-Transform Method

<div><p>Flow-through respirometry systems provide accurate measurement of gas exchange over long periods of time. However, these systems have limitations in tracking rapid changes. When an animal infuses a metabolic gas into the respirometry chamber in a short burst, diffusion and airflow in the chamber gradually alter the original signal before it arrives at the gas analyzer. For single or multiple bursts, the recorded signal is smeared or mixed, which may result in dramatically altered recordings compared to the emitted signal. Recovering the original metabolic signal is a difficult task because of the inherent ill conditioning problem. Here, we present two new methods to recover the fast dynamics of metabolic patterns from recorded data. We first re-derive the equations of the well-known Z-transform method (ZT method) to show the source of imprecision in this method. Then, we develop a new model of analysis for respirometry systems based on the experimentally determined impulse response, which is the response of the system to a very short unit input. As a result, we present a major modification of the ZT method (dubbed the ‘EZT method’) by using a new model for the impulse response, enhancing its precision to recover the true metabolic signals. The second method, the generalized Z-transform (GZT) method, was then developed by generalizing the EZT method; it can be applied to any flow-through respirometry system with any arbitrary impulse response. Experiments verified that the accuracy of recovering the true metabolic signals is significantly improved by the new methods. These new methods can be used more broadly for input estimation in variety of physiological systems.</p></div

Recovering signals in physiological systems with large datasets

In many physiological studies, variables of interest are not directly accessible, requiring that they be estimated indirectly from noisy measured signals. Here, we introduce two empirical methods to estimate the true physiological signals from indirectly measured, noisy data. The first method is an extension of Tikhonov regularization to large-scale problems, using a sequential update approach. In the second method, we improve the conditioning of the problem by assuming that the input is uniform over a known time interval, and then use a least-squares method to estimate the input. These methods were validated computationally and experimentally by applying them to flow-through respirometry data. Specifically, we infused CO2 in a flow-through respirometry chamber in a known pattern, and used the methods to recover the known input from the recorded data. The results from these experiments indicate that these methods are capable of sub-second accuracy. We also applied the methods on respiratory data from a grasshopper to investigate the exact timing of abdominal pumping, spiracular opening, and CO2 emission. The methods can be used more generally for input estimation of any linear system

Data from: Tracheal compression in pupae of the beetle Zophobas morio

Insects that are small or exhibit low metabolic rates are considered to not require active ventilation to augment diffusive gas exchange. Some pupae with low metabolic rates exhibit abdominal pumping, a behaviour that is known to drive tracheal ventilation in the adults of many species. However, previous work on pupae suggests that abdominal pumping may serve a non-respiratory role. To study the role of abdominal pumping in pupa of the beetle Zophobas morio, we visualized tracheal dynamics with X-rays while simultaneously measuring haemolymph pressure, abdominal movement, and CO2 emission. Pupae exhibited frequent tracheal compressions that were coincident with both abdominal pumping and pulsation of pressure in the haemolymph. However, more than 63% of abdominal pumping events occurred without any tracheal collapse and hence ventilation, suggesting that the major function of the abdominal pump is not respiratory. In addition, this study shows that the kinematics of abdominal pumping can be used to infer the status of the spiracles and internal behaviour of the tracheal system

Recovery of the actual metabolic rate of a beetle.

<p>ZT, EZT, and GZT methods were applied on the recorded respirometry data of a <i>Zophobas morio</i> adult and the recovered instantaneous signals compared with a threshold line to find the open and closed phase of the spiracles.</p

Comparison of ZT, EZT, and GZT methods.

<p>The injected input of 100 ppm CO₂ with duration of 200 ms into the 28 mL respirometry chamber is estimated from the output signal using different methods.</p

Comparing different methods for recovering the CO₂ input.

<p>CO₂ was infused with rectangular pulses with different frequency and duration into the respirometry chamber in two flow rates of 250 and 500 mL/min and the output was recorded (A). ZT, EZT, and GZT methods were used to recover the CO₂ inputs from the recorded data (B and C). The results indicate that the precision of GZT method is significantly higher than the ZT and EZT methods and the EZT is more precise than the ZT method.</p

Experimental setup to determine the impulse response of a flow through respirometry system.

<p>The impulse response of each respirometry setup was found by infusing a pulse of CO₂ with the duration of 100 ms by means of the picospritzer, roughly in the location where an animal would be. The recorded output was normalized to find the impulse response.</p