Not in the Hardware Aisle, Please : Same-Sex Marriage, Anti-Gay Activism and My Fabulous Gay Wedding

My Fabulous Gay Wedding was intended from its conception to be “a hot topic, a controversial topic, a topic filled with lots of opportunity for emotional moments.” This article traces a number of contemporary discourses around same-sex marriage that are illuminated, albeit not clarified, by responses to the show, including a boycott of Canadian Tire, a purported sponsor, by a number of right-wing, anti-gay groups. However, the questions the show raises exceed the media’s simplistic binary of “lesbian and gay rights” v. “religious rights” and involve interrogating what, exactly, is being represented on screen, and whether or not same-sex weddings interpellate lesbian and gay couples into a form of heteronormativity.My Fabulous Gay Wedding [Mon merveilleux mariage gai] était conçu dès le départ pour être « un sujet chaud, un sujet de controverse, un sujet rempli de possibilités d’instants d’émotion ». Cet article esquisse un certain nombre de discours contemporains portant sur les mariages entre gens du même sexe qui se trouvent illuminés, quoique pas totalement clarifiés, par les réactions à cette émission, incluant un boycott de Canadian Tire, commanditaire présumé, par un certain nombre de groupes conservateurs anti-gais. Cependant, les questions que soulève cette émission dépassent la scission binaire simpliste des médias entre les « droits des gais et des lesbiennes » versus les « droits religieux » et implique que l’on s’interroge sur ce qui est représenté à l’écran exactement et si, oui ou non, les mariages entre gens du même sexe interpellent les couples de gais et de lesbiennes pour qu’ils entrent dans une forme d’hétéronormativité

Flood Inundation Mapping

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Dietary Cucumis melo Reduces Markers of Muscle and Articular Inflammation Following High-intensity Exercise in Horses

We evaluated the antioxidative and anti-inflammatory potential of daily oral supplementation with a proprietary powdered Cucumis melo pulp (CMP) on exercise-induced markers of articular and muscular oxidative stress and inflammation in 12 horses. Horses performed a high-intensity exercise test immediately prior to, and then following, 3 weeks of daily supplementation of 1 g powdered CMP (CMP; n=8). Controls (Co; n=8) underwent the same exercise and sampling regime but were not supplemented. Blood and synovial fluid (SF) samples were taken 24 h prior to exercise (BL), and at 1 and 24 h following exercise. Plasma and SF were analysed for prostaglandin E2 (PGE2), total antioxidant status (TAS), nitrite and superoxide dismutase (SOD) activity. SF was analysed for glycosaminoglycans (GAG), and plasma was analysed for thiobarbituric acid reactive substances (TBARS). Comparisons were made using repeated measures with the initial exercise test as a covariate. There was an increase in SF SOD activity in the CMP group. Compared to Co at 1 h, CMP reduced nitrite and GAG in SF, as well as maintained plasma TAS and lymphocyte levels. At 24 h, plasma PGE2 and creatine kinase were lower in horses receiving CMP. Three weeks of supplementation with CMP reduced markers of articular and skeletal muscle oxidative stress and inflammation in response to high-intensity exercise in horses. Nutritive antioxidants may provide a useful adjunct to the daily nutrition plan of horses undergoing regular exercise training and competition

Wheatgrass extract has chondroprotective and anti-inflammatory effects on porcine cartilage

Lameness is a commonly observed disorder in sows and negatively impacts both animal welfare and the profitability of the pig sector. The purpose of this study was to determine anti-inflammatory and/or chondroprotective effects of wheatgrass (WG) on porcine cartilage explants stimulated with lipopolysaccharide (LPS). Explants were aseptically prepared from the intercarpal joints of nine market-weight pigs and placed in culture at 37°C for a total of 120 hours. For the final 96 hours, explants were conditioned with an aqueous extract of WG (0, 5 or 15 μg/mL), and for the final 48 hours explants were stimulated with LPS (0 or 10 µg/mL). Media was removed and replaced every 24 hours. Samples from the final 48 hours were analyzed for biomarkers of cartilage inflammation [prostaglandin E2 (PGE2) and nitric oxide (NO)] and cartilage structure [glycosaminoglycan (GAG)], and cartilage explants were stained for an estimate of cell viability. Stimulation of explants with LPS significantly increased media concentrations of PGE2, GAG and NO compared with that from unstimulated explants. LPS stimulation did not significantly affect cell viability. Conditioning of explants with WG (5 μg/mL) significantly reduced LPS-stimulated cartilage release of PGE2, NO, and GAG (5 and 15 μg/mL), without impairing chondrocyte viability. These data provide evidence for a non-cytotoxic chondroprotective and anti-inflammatory effect of WG extract in cartilage and suggest a role of WG in protection against cartilage breakdown, inflammation, and pain associated with osteoarthritis

Safety of dietary camelina oil supplementation in healthy, adult dogs

This study aimed to determine whether camelina oil is safe for use in canine diets, using canola oil and flax oil as controls, as they are similar and generally regarded as safe (GRAS) for canine diets. A total of thirty privately-owned adult dogs of various breeds (17 females; 13 males), with an average age of 7.2 ± 3.1 years (mean ± SD) and a body weight (BW) of 27.4 ± 14.0 kg were used. After a 4-week wash-in period using sunflower oil and kibble, the dogs were blocked by breed, age, and size and were randomly allocated to one of three treatment oils (camelina (CAM), flax (FLX), or canola (OLA)) at a level of 8.2 g oil/100 g total dietary intake. Body condition score (BCS), BW, food intake (FI), and hematological and select biochemical parameters were measured at various timepoints over a 16-week feeding period. All of the data were analyzed with ANOVA using the PROC GLIMMIX of SAS. No biologically significant differences were seen between the treatment groups in terms of BW, BCS, FI, and hematological and biochemical results. Statistically significant differences noted among some serum biochemical results were considered small and were due to normal biological variation. These results support the conclusion that camelina oil is safe for use in canine nutrition

Influence of a Bi surfactant on Sb incorporation in InAsSb alloys

The influence of using a Bi surfactant during the growth of InAsSb on the composition was examined, and it was found that increasing Bi flux on the surface during growth inhibits the incorporation of Sb. Analysis of the data via a kinetic model of anion incorporation shows that surface Bi acts as a catalyst for InAs formation, thus inhibiting Sb incorporation

Low dose influenza virus challenge in the ferret leads to increased virus shedding and greater sensitivity to oseltamivir

Ferrets are widely used to study human influenza virus infection. Their airway physiology and cell receptor distribution makes them ideal for the analysis of pathogenesis and virus transmission, and for testing the efficacy of anti-influenza interventions and vaccines. The 2009 pandemic influenza virus (H1N1pdm09) induces mild to moderate respiratory disease in infected ferrets, following inoculation with 106 plaque-forming units (pfu) of virus. We have demonstrated that reducing the challenge dose to 102 pfu delays the onset of clinical signs by 1 day, and results in a modest reduction in clinical signs, and a less rapid nasal cavity innate immune response. There was also a delay in virus production in the upper respiratory tract, this was up to 9-fold greater and virus shedding was prolonged. Progression of infection to the lower respiratory tract was not noticeably delayed by the reduction in virus challenge. A dose of 104 pfu gave an infection that was intermediate between those of the 106 pfu and 102 pfu doses. To address the hypothesis that using a more authentic low challenge dose would facilitate a more sensitive model for antiviral efficacy, we used the well-known neuraminidase inhibitor, oseltamivir. Oseltamivir-treated and untreated ferrets were challenged with high (106 pfu) and low (102 pfu) doses of influenza H1N1pdm09 virus. The low dose treated ferrets showed significant delays in innate immune response and virus shedding, delayed onset of pathological changes in the nasal cavity, and reduced pathological changes and viral RNA load in the lung, relative to untreated ferrets. Importantly, these observations were not seen in treated animals when the high dose challenge was used. In summary, low dose challenge gives a disease that more closely parallels the disease parameters of human influenza infection, and provides an improved pre-clinical model for the assessment of influenza therapeutics, and potentially, influenza vaccines

Development of an open-source, flexible framework for complex inter-institutional disparate data sharing and collaboration

Clinical information, “-omic” datasets, and tissue samples are difficult to harmonize and manage for data mining. We have developed a platform for storing clinical research data while providing access to associated data from other information stores. Data on 34 metrics from 11,000 neuroblastoma patients were instantiated into a database. The Django web framework was used to create a model for rapid development of tools and views with a front-end interface for generating complex queries. Working with Nationwide Children’s Hospital, we can now consume their tissue inventory data through an API. The end-user sees the number of patients who both match their search and have tissue available. Since initial implementation, the current tasks revolve around developing a governance structure and the necessary data use agreements. Efforts now are to (1) update the data with 5000 more patients, and (2) link to genomic data stores, facilitating disparate data acquisition for research studies

Advancing human capabilities for water security: A relational approach

We argue that a relational water security framework informed by the capabilities approach offers new ways to consider politics and cultures of water. Each dimension allows us to better contextualize water security beyond just an object (H2O) to be secured for a certain population. Instead, the relational perspective demands a fuller consideration of the political structures and processes through which water is secured, with emphasis on the social relations of access as opposed to simply the politics around water supply. We also attend to cultural dimensions, such as the meanings of water and customary practices that are not easily captured by standardized metrics. By including these dimensions, we necessarily broaden analytical space to evaluate water security as a relational and dynamic process tied to lived experience rather than as solely parameterized conditions in relation to access, quality, or availability of water. We first move to explain our broader conceptualization of water security as linked to human capabilities, then explore in more detail the specific engagements with politics and culture in the sections that follow

Protection against LPS-induced cartilage inflammation and degradation provided by a biological extract of Mentha spicata

<p>Abstract</p> <p>Background</p> <p>A variety of mint [<it>Mentha spicata</it>] has been bred which over-expresses Rosmarinic acid (RA) by approximately 20-fold. RA has demonstrated significant anti-inflammatory activity <it>in vitro </it>and in small rodents; thus it was hypothesized that this plant would demonstrate significant anti-inflammatory activity <it>in vitro</it>. The objectives of this study were: a) to develop an <it>in vitro </it>extraction procedure which mimics digestion and hepatic metabolism, b) to compare anti-inflammatory properties of High-Rosmarinic-Acid <it>Mentha spicata </it>(HRAM) with wild-type control <it>M. spicata </it>(CM), and c) to quantify the relative contributions of RA and three of its hepatic metabolites [ferulic acid (FA), caffeic acid (CA), coumaric acid (CO)] to anti-inflammatory activity of HRAM.</p> <p>Methods</p> <p>HRAM and CM were incubated in simulated gastric and intestinal fluid, liver microsomes (from male rat) and NADPH. Concentrations of RA, CA, CO, and FA in simulated digest of HRAM (HRAM_sim) and CM (CM_sim) were determined (HPLC) and compared with concentrations in aqueous extracts of HRAM and CM. Cartilage explants (porcine) were cultured with LPS (0 or 3 μg/mL) and test article [HRAM_sim(0, 8, 40, 80, 240, or 400 μg/mL), or CM_sim(0, 1, 5 or 10 mg/mL), or RA (0.640 μg/mL), or CA (0.384 μg/mL), or CO (0.057 μg/mL) or FA (0.038 μg/mL)] for 96 h. Media samples were analyzed for prostaglandin E₂(PGE₂), interleukin 1β (IL-1), glycosaminoglycan (GAG), nitric oxide (NO) and cell viability (differential live-dead cell staining).</p> <p>Results</p> <p>RA concentration of HRAM_simand CM_simwas 49.3 and 0.4 μg/mL, respectively. CA, FA and CO were identified in HRAM_simbut not in aqueous extract of HRAM. HRAM_sim(≥ 8 μg/mL) inhibited LPS-induced PGE₂and NO; HRAM_sim(≥ 80 μg/mL) inhibited LPS-induced GAG release. RA inhibited LPS-induced GAG release. No anti-inflammatory or chondroprotective effects of RA metabolites on cartilage explants were identified.</p> <p>Conclusions</p> <p>Our biological extraction procedure produces a substance which is similar in composition to post-hepatic products. HRAM_simis an effective inhibitor of LPS-induced inflammation in cartilage explants, and effects are primarily independent of RA. Further research is needed to identify bioactive phytochemical(s) in HRAM_sim.</p