Can visualization of contributions support fairness in collaboration? Findings from meters in an online game

In this paper we consider how visualizations might support judgements about fairness in collaborative work. We present a qualitative investigation of meters, existing artefacts that enable awareness of contributions in the online game World of Warcraft. Through in-depth interviews with game players, we draw attention to the value of meters as tools for self-reflection and group evaluation. Yet we also describe problematic consequences that arise as a result of meters, distinguishing their usage as in-the-moment awareness tools from instruments used after the fact to apportion credit and blame. We argue that representations like meters may be enough to provoke initial questions about fairness, but are likely to undermine the efforts of collaborators if they fail to combine a set of indices that reflect important aspects of individual work in the context of team activity. We consider broader lessons for the way in which future designs might aim to support fairness in collaborative systems, pointing to multidimensional mechanisms adapted specifically to real-time or retrospective usage

His/Met heme ligation in the PioA outer membrane cytochrome enabling light-driven extracellular electron transfer by Rhodopseudomonas palustris TIE-1

A growing number of bacterial species are known to move electrons across their cell envelopes. Naturally this occurs in support of energy conservation and carbon-fixation. For biotechnology it allows electron exchange between bacteria and electrodes in microbial fuel cells and during microbial electrosynthesis. In this context Rhodopseudomonas palustris TIE-1 is of much interest. These bacteria respond to light by taking electrons from their external environment, including electrodes, to drive CO2-fixation. The PioA cytochrome, that spans the bacterial outer membrane, is essential for this electron transfer and yet little is known about its structure and electron transfer properties. Here we reveal the ten c-type hemes of PioA are redox active across the window +250 to -400 mV versus Standard Hydrogen Electrode and that the hemes with most positive reduction potentials have His/Met and His/H2O ligation. These chemical and redox properties distinguish PioA from the more widely studied family of MtrA outer membrane decaheme cytochromes with ten His/His ligated hemes. We predict a structure for PioA in which the hemes form a chain spanning the longest dimension of the protein, from Heme 1 to Heme 10. Hemes 2, 3 and 7 are identified as those most likely to have His/Met and/or His/H2O ligation. Sequence analysis suggests His/Met ligation of Heme 2 and/or 7 is a defining feature of decaheme PioA homologs from over 30 different bacterial genera. His/Met ligation of Heme 3 appears to be less common and primarily associated with PioA homologs from purple non-sulphur bacteria belonging to the alphaproteobacteria class

Gender differences in the impact of population-level alcohol policy interventions: evidence synthesis of systematic reviews

Background: Consistent review-level evidence supports the effectiveness of population-level alcohol policies in reducing alcohol-related harms. Such policies interact with well-established social, cultural and biological differences in how men and women perceive, relate to and use alcohol, and with wider inequalities, in ways which may give rise to gender differences in policy effectiveness.  Aims: To examine the extent to which gender-specific data and analyses were considered in, and are available from, systematic reviews of population-level alcohol policy interventions, and where possible, to conduct a narrative synthesis of relevant data.  Methods: A prior systematic ‘review of reviews’ of population level alcohol interventions 2002-2012 was updated to May 2014, all gender-relevant data extracted, and the level and quality of gender reporting assessed. A narrative synthesis of extracted findings was conducted.  Results: Sixty-three systematic reviews, covering ten policy areas, were included. Five reviews (8%) consistently provided information on baseline participation by gender for each individual study in the review and twenty-nine (46%) reported some gender-specific information on the impact of the policies under consideration. Specific findings include evidence of possible gender differences in the impact of and exposure to alcohol marketing, and a failure to consider potential unintended consequences and harm to others in most reviews.  Conclusions: Gender is poorly reported in systematic reviews of population-level interventions to reduce alcohol-related harm, hindering assessment of the intended and unintended effects of such policies on women and men

Immunosuppressive potential of human amnion epithelial cells in the treatment of experimental autoimmune encephalomyelitis

BACKGROUND: Multiple sclerosis (MS) is an autoimmune inflammatory disease of the central nervous system (CNS). In recent years, it has been found that cells such as human amnion epithelial cells (hAECs) have the ability to modulate immune responses in vitro and in vivo and can differentiate into multiple cell lineages. Accordingly, we investigated the immunoregulatory effects of hAECs as a potential therapy in an MS-like disease, EAE (experimental autoimmune encephalomyelitis), in mice. METHODS: Using flow cytometry, the phenotypic profile of hAECs from different donors was assessed. The immunomodulatory properties of hAECs were examined in vitro using antigen-specific and one-way mixed lymphocyte proliferation assays. The therapeutic efficacy of hAECs was examined using a relapsing-remitting model of EAE in NOD/Lt mice. T cell responsiveness, cytokine secretion, T regulatory, and T helper cell phenotype were determined in the peripheral lymphoid organs and CNS of these animals. RESULTS: In vitro, hAECs suppressed both specific and non-specific T cell proliferation, decreased pro-inflammatory cytokine production, and inhibited the activation of stimulated T cells. Furthermore, T cells retained their naïve phenotype when co-cultured with hAECs. In vivo studies revealed that hAECs not only suppressed the development of EAE but also prevented disease relapse in these mice. T cell responses and production of the pro-inflammatory cytokine interleukin (IL)-17A were reduced in hAEC-treated mice, and this was coupled with a significant increase in the number of peripheral T regulatory cells and naïve CD4+ T cells. Furthermore, increased proportions of Th2 cells in the peripheral lymphoid organs and within the CNS were observed. CONCLUSION: The therapeutic effect of hAECs is in part mediated by inducing an anti-inflammatory response within the CNS, demonstrating that hAECs hold promise for the treatment of autoimmune diseases like MS

Climatology of surface meteorology, surface fluxes, cloud fraction, and radiative forcing over the southeast Pacific from buoy observations

Author Posting. © American Meteorological Society, 2009. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Climate 22 (2009): 5527–5540, doi:10.1175/2009JCLI2961.1.A 5-yr climatology of the meteorology, including boundary layer cloudiness, for the southeast Pacific region is presented using observations from a buoy located at 20°S, 85°W. The sea surface temperature and surface air temperature exhibit a sinusoidal seasonal cycle that is negatively correlated with surface pressure. The relative humidity, wind speed, and wind direction show little seasonal variability. But the advection of cold and dry air from the southeast varies seasonally and is highly correlated with the latent heat flux variations. A simple model was used to estimate the monthly cloud fraction using the observed surface downwelling longwave radiative flux and surface meteorological parameters. The annual cycle of cloud fraction is highly correlated to that of S. A. Klein: lower-tropospheric stability parameter (0.87), latent heat flux (−0.59), and temperature and moisture advection (0.60). The derived cloud fraction compares poorly with the International Satellite Cloud Climatology Project (ISCCP)-derived low-cloud cover but compares well (0.86 correlation) with ISCCP low- plus middle-cloud cover. The monthly averaged diurnal variations in cloud fraction show marked seasonal variability in the amplitude and temporal structure. The mean annual cloud fraction is lower than the mean annual nighttime cloud fraction by about 9%. Annual and diurnal cycles of surface longwave and shortwave cloud radiative forcing were also estimated. The longwave cloud radiative forcing is about 45 W m−2 year-round, but, because of highly negative shortwave cloud radiative forcing, the net cloud radiative forcing is always negative with an annual mean of −50 W m−2.This research was supported by the Climate Prediction Program for the Americas (CPPA) of NOAA’s Climate Program Office. The Stratus Ocean Reference Station at 20°S, 85°W is supported by NOAA’s Climate Observation Program