Effects of Diversity on Emerging Infectious Diseases of Amphibians

Species declines and extinctions have been recorded across taxa as evidence of an ongoing global biodiversity crisis. Amphibians are at the forefront of these declines with nearly one third of amphibian species estimated to be at risk of extinction. While many factors contribute to population declines and extinctions, the role of disease is of particular concern to amphibians which are hosts to multiple globally distributed pathogens such as the fungal pathogen Batrachochytrium dendrobatidis (Bd), the dsDNA virus, ranavirus and trematode parasites, all of which can affect host populations. Amphibian disease-mediated population declines and extinctions have drawn special attention to the relationships between disease and diversity in general, as an understanding of these relationships is crucial for conservation and management efforts. Host species vary in their competence (effectiveness of transmission) to specific pathogens. As host diversity increases, so does the likelihood of encountering an especially competent or incompetent host, which can alter disease dynamics in the greater community. Prior examinations of amphibian host diversity suggest that increased host diversity reduces disease risks of Bd and trematodes in some systems but the generality of these effect is still debated. Far less is known about the role of host diversity and ranavirus dynamics. Changes in pathogen diversity also alter disease dynamics, as pathogens interact via their effects on a host and its immune system. In amphibian systems, co-infection often increases disease burdens. although this is not always the case. Here, using a series of experiments, I examined the interrelationships among diversity and disease in amphibians, with a focus on the Western toad (Anaxyrus boreas) as host. Western toads have experienced range reductions and population declines at high altitude sites in the Oregon Cascade Range. In chapter two I paired laboratory and outdoor mesocosm experiments to examine the relationships of host diversity with ranavirus. In the laboratory experiment I found that after ranavirus exposure, only the Pacific chorus frog (Pseudacris regilla) experienced increased mortality when exposed to ranavirus. In the mesocosm experiment, like the laboratory study, the addition of ranavirus exposed conspecifics led to reduced host survival. However, in the presence of P. regilla amphibian assemblages experienced nearly complete mortality of all species, including A. boreas and the Cascades frog (Rana cascadae). In chapter three I examined the effects of host diversity on Bd disease dynamics. Previous laboratory studies in my system suggested a protective effect of diversity on Bd disease risk. In an outdoor mesocosm experiment I found that communities of only A. boreas experienced reduced survival after addition of Bd exposed conspecifics. However when all three host species were present, survival was not different from controls, providing evidence that the reduction of Bd disease risk associated with increased host diversity is robust to changes in scale and ecosystem complexity. In chapter four I experimentally altered both host and pathogen diversity in a factorial laboratory experiment which elicited a range of responses. I found increased variation in growth in the two days following experimental pathogen exposure, with the direction and strength of the effect modulated by host-pathogen combination. In the absence of other host species A. boreas, had increased mortality when simultaneously exposed to both Bd and trematodes. This dissertation provides evidence of the impacts of diversity on amphibian disease outcomes and highlights the role of community composition in wildlife disease dynamics. This work suggests that to fully appreciate the dynamics of wildlife disease we must consider all interacting species in a community. I provide evidence that increased host diversity may reduce Bd disease risks in a community, while those same changes to host diversity in ranavirus exposed community can lead to collapse of amphibian populations. Further I have shown differences between laboratory and field experiments that suggest pairing diversity studies at different scales is important in the study of wildlife disease

Coupling thermodynamics and digital image models to simulate hydration and microstructure development of portland cement pastes

Equilibrium thermodynamic calculations, coupled to a kinetic model for the dissolution rates of clinker phases, have been used in recent years to predict time-dependent phase assemblages in hydrating cement pastes. We couple this approach to a 3D microstructure model to simulate microstructure development during the hydration of ordinary portland cement pastes. The combined simulation tool uses a collection of growth/dissolution rules to approximate a range of growth modes at material interfaces, including growth by weighted mean curvature and growth by random aggregation. The growth rules are formulated for each type of material interface to capture the kinds of cement paste microstructure changes that are typically observed. We make quantitative comparisons between simulated and observed microstructures for two ordinary portland cements, including bulk phase analyses and two-point correlation functions for various phases. The method is also shown to provide accurate predictions of the heats of hydration and 28 day mortar cube compressive strengths. The method is an attractive alternative to the cement hydration and microstructure model CEMHYD3D because it has a better thermodynamic and kinetic basis and because it is transferable to other cementitious material system

Study of Sabatier Catalyst Performance for a Mars ISRU Propellant Production Plant

NASA is currently developing technologies for use in the field of in-situ resource utilization (ISRU). One of the technologies being advanced is the Sabatier, or methanation, reactor which converts carbon dioxide and hydrogen into methane gas and water at high temperatures. This paper discusses the catalyst life and performance issues for these reactors that would be expected on Mars and describes the test methods employed and observed results. The various catalysts were tested in their capacity for the continuous production of methane gas via the Sabatier reaction and the possible effects of launch vibration loads, exposure to liquid water, particulate contamination, and chemical contamination to the overall observed reaction efficacy of the catalysts evaluated

Processing EOS MLS Level-2 Data

A computer program performs level-2 processing of thermal-microwave-radiance data from observations of the limb of the Earth by the Earth Observing System (EOS) Microwave Limb Sounder (MLS). The purpose of the processing is to estimate the composition and temperature of the atmosphere versus altitude from .8 to .90 km. "Level-2" as used here is a specialists f term signifying both vertical profiles of geophysical parameters along the measurement track of the instrument and processing performed by this or other software to generate such profiles. Designed to be flexible, the program is controlled via a configuration file that defines all aspects of processing, including contents of state and measurement vectors, configurations of forward models, measurement and calibration data to be read, and the manner of inverting the models to obtain the desired estimates. The program can operate in a parallel form in which one instance of the program acts a master, coordinating the work of multiple slave instances on a cluster of computers, each slave operating on a portion of the data. Optionally, the configuration file can be made to instruct the software to produce files of simulated radiances based on state vectors formed from sets of geophysical data-product files taken as input

Hubble Space Telescope Imaging of the Circumnuclear Environments of the CfA Seyfert Galaxies: Nuclear Spirals and Fueling

We present archival Hubble Space Telescope images of the nuclear regions of 43 of the 46 Seyfert galaxies found in the volume-limited, spectroscopically-complete CfA Redshift Survey sample. Using an improved method of image contrast enhancement, we create detailed high-quality structure maps that allow us to study the distributions of dust, star clusters, and emission-line gas in the circumnuclear regions (100-1000 pc scales) and in the associated host galaxy. Essentially all of these Seyfert galaxies have circumnuclear dust structures with morphologies ranging from grand-design two-armed spirals to chaotic dusty disks. In most Seyferts there is a clear physical connection between the nuclear dust spirals on hundreds of parsec scales and large-scale bars and spiral arms in the host galaxies proper. These connections are particularly striking in the interacting and barred galaxies. Such structures are predicted by numerical simulations of gas flows in barred and interacting galaxies, and may be related to the fueling of AGN by matter inflow from the host galaxy disks. We see no significant differences in the circumnuclear dust morphologies of Seyfert 1s and 2s, and very few Seyfert 2 nuclei are obscured by large-scale dust structures in the host galaxies. If Seyfert 2s are obscured Seyfert 1s, then the obscuration must occur on smaller scales than those probed by HST.Comment: 29 pages, 11 figures. Accepted for publication in the ApJ. For high-resolution figures see http://www.astronomy.ohio-state.edu/~pogge/CfA

Optical characterization of porous alumina from vacuum ultraviolet to mid-infrared

Porous alumina was fabricated and optically characterized over a wide spectral range. Layers were formed electrochemically in oxalic acid solution from 10-μm-thick aluminum films evaporated onto silicon wafers. The layer formation was monitored with in situ spectroscopic ellipsometry in the visible and near-infrared wavelength range to accurately determine the thickness and dielectric functions. Anisotropy due to the columnar nature of the porous structure was determined using optical modeling. The porous alumina layer was found to have a small but significant absorption tail throughout the visible region. Atomic force microscopy and scanning electron microscopy were used throughout the process to assess the quality of pore formation. The mean pore center-to-center spacing was approximately 100 nm with thicknesses up to 5 μm. The infrared spectra revealed absorption peaks previously seen in ceramic alumina and peaks not associated with bulk alumina

How do field of view and resolution affect the information content of panoramic scenes for visual navigation? A computational investigation

The visual systems of animals have to provide information to guide behaviour and the informational requirements of an animal’s behavioural repertoire are often reflected in its sensory system. For insects, this is often evident in the optical array of the compound eye. One behaviour that insects share with many animals is the use of learnt visual information for navigation. As ants are expert visual navigators it may be that their vision is optimised for navigation. Here we take a computational approach in asking how the details of the optical array influence the informational content of scenes used in simple view matching strategies for orientation. We find that robust orientation is best achieved with low-resolution visual information and a large field of view, similar to the optical properties seen for many ant species. A lower resolution allows for a trade-off between specificity and generalisation for stored views. Additionally, our simulations show that orientation performance increases if different portions of the visual field are considered as discrete visual sensors, each giving an independent directional estimate. This suggests that ants might benefit by processing information from their two eyes independently

Study of Sabatier Catalyst Performance for a Mars ISRU Propellant Production Plant

NASA is currently developing technologies for use in the field of in-situ resource utilization (ISRU). One of the technologies being advanced is the Sabatier, or methanation, reactor which converts carbon dioxide and hydrogen into methane gas and water at high temperatures. This paper discusses the catalyst life and performance issues for these reactors that would be expected on Mars and describes the test methods employed and observed results. The various catalysts were tested in their capacity for the continuous production of methane gas via the Sabatier reaction and the possible effects of launch vibration loads, exposure to liquid water, particulate contamination, and chemical contamination to the overall observed reaction efficacy of the catalysts evaluated

Neonatal-onset multisystem inflammatory disease responsive to interleukin-1 beta inhibition

BACKGROUND:Neonatal-onset multisystem inflammatory disease is characterized by fever, urticarial rash, aseptic meningitis, deforming arthropathy, hearing loss, and mental retardation. Many patients have mutations in the cold-induced autoinflammatory syndrome 1 (CIAS1) gene, encoding cryopyrin, a protein that regulates inflammation.METHODS:We selected 18 patients with neonatal-onset multisystem inflammatory disease (12 with identifiable CIAS1 mutations) to receive anakinra, an interleukin-1-receptor antagonist (1 to 2 mg per kilogram of body weight per day subcutaneously). In 11 patients, anakinra was withdrawn at three months until a flare occurred. The primary end points included changes in scores in a daily diary of symptoms, serum levels of amyloid A and C-reactive protein, and the erythrocyte sedimentation rate from baseline to month 3 and from month 3 until a disease flare.RESULTS:All 18 patients had a rapid response to anakinra, with disappearance of rash. Diary scores improved (P<0.001) and serum amyloid A (from a median of 174 mg to 8 mg per liter), C-reactive protein (from a median of 5.29 mg to 0.34 mg per deciliter), and the erythrocyte sedimentation rate decreased at month 3 (all P<0.001), and remained low at month 6. Magnetic resonance imaging showed improvement in cochlear and leptomeningeal lesions as compared with baseline. Withdrawal of anakinra uniformly resulted in relapse within days; retreatment led to rapid improvement. There were no drug-related serious adverse events.CONCLUSIONS:Daily injections of anakinra markedly improved clinical and laboratory manifestations in patients with neonatal-onset multisystem inflammatory disease, with or without CIAS1 mutations

Physical drivers facilitating a toxigenic cyanobacterial bloom in a major Great Lakes tributary

The Maumee River is the primary source for nutrients fueling seasonal Microcystis-dominated blooms in western Lake Erie\u27s open waters though such blooms in the river are infrequent. The river also serves as source water for multiple public water systems and a large food services facility in northwest Ohio. On 20 September 2017, an unprecedented bloom was reported in the Maumee River estuary within the Toledo metropolitan area, which triggered a recreational water advisory. Here we (1) explore physical drivers likely contributing to the bloom\u27s occurrence, and (2) describe the toxin concentration and bacterioplankton taxonomic composition. A historical analysis using 10-years of seasonal river discharge, water level, and local wind data identified two instances when high-retention conditions occurred over ≥ 10 d in the Maumee River estuary: in 2016 and during the 2017 bloom. Observation by remote sensing imagery supported the advection of cyanobacterial cells into the estuary from the lake during 2017 and the lack of an estuary bloom in 2016 due to a weak cyanobacterial bloom in the lake. A rapid-response survey during the 2017 bloom determined levels of the cyanotoxins, specifically microcystins, in excess of recreational contact limits at sites within the lower 20 km of the river while amplicon sequencing found these sites were dominated by Microcystis. These results highlight the need to broaden our understanding of physical drivers of cyanobacterial blooms within the interface between riverine and lacustrine systems, particularly as such blooms are expected to become more prominent in response to a changing climate