Cloud Condensation in Titan's Lower Stratosphere

A 1-D condensation model is developed for the purpose of reproducing ice clouds in Titan's lower stratosphere observed by the Composite Infrared Spectrometer (CIRS) onboard Cassini. Hydrogen cyanide (HCN), cyanoacetylene (HC3N), and ethane (C2H6) vapors are treated as chemically inert gas species that flow from an upper boundary at 500 km to a condensation sink near Titan's tropopause (-45 km). Gas vertical profiles are determined from eddy mixing and a downward flux at the upper boundary. The condensation sink is based upon diffusive growth of the cloud particles and is proportional to the degree of supersaturation in the cloud formation regIOn. Observations of the vapor phase abundances above the condensation levels and the locations and properties of the ice clouds provide constraints on the free parameters in the model. Vapor phase abundances are determined from CIRS mid-IR observations, whereas cloud particle sizes, altitudes, and latitudinal distributions are derived from analyses of CIRS far-IR observations of Titan. Specific cloud constraints include: I) mean particle radii of2-3 J.lm inferred from the V6 506 cm- band of HC3N, 2) latitudinal abundance distributions of condensed nitriles, inferred from a composite emission feature that peaks at 160/cm , and 3) a possible hydrocarbon cloud layer at high latitudes, located near an altitude of 60 km, which peaks between 60 and 80 cm l . Nitrile abundances appear to diminish substantially at high northern latitudes over the time period 2005 to 2010 (northern mid winter to early spring). Use of multiple gas species provides a consistency check on the eddy mixing coefficient profile. The flux at the upper boundary is the net column chemical production from the upper atmosphere and provides a constraint on chemical pathways leading to the production of these compounds. Comparison of the differing lifetimes, vapor phase transport, vapor phase loss rate, and particle sedimentation, sheds light on temporal stability of the clouds

Stratospheric aerosols from CH 4 photochemistry on Neptune

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/95495/1/grl4442.pd

Neptune's deep atmosphere revealed

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/94842/1/grl4440.pd

Methane photochemistry and haze production on Neptune

A numerical model was used to study methane photochemistry in the stratosphere of Neptune. The observed mixing ratio of methane, 2%, forces photolysis to occur near the CH4 homopause. For an assumed nominal value of the eddy mixing coefficient of 106 cm2 sec-1 at the CH4 homopause, the predicted average mixing ratios of C2H6 and C2H2, 1.5 x 10-6 and 6 x 10-7, respectively, agree well with observations in the infrared. The acetylene and ethane abundances are weakly dependent upon the strength of the eddy mixing and directly proportional to it. Haze production from methane photochemistry results from the formation of hydrocarbon ices and polyacetylenes. The calculated mixing ratios of C2H6, C2H2 are large enough to cause condensation to their respective ices near the tropopause. These hazes are capable of providing the necessary aerosol optical depth at the appropriate pressure levels required by observations of Neptune in the visible and near IR. Polyacetylene formation from C2H2 photolysis is limited by the low quantum yield of dissociation for acetylene, efficient recycling of its photolysis products by the other hydrocarbons, and the greatly reduced solar flux at Neptune. Comparisons of model predictions to Uranus show both a lower ratio of polyacetylene production to hydrocarbon ice and a lower likelihood of UV postprocessing of the acetylene ice to polymers on Neptune compared to Uranus. This is in agreement with the observed difference in the single scattering albedo of the stratospheric aerosols in the visible between Uranus and Neptune, with the aerosols on Neptune being brighter.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/27284/1/0000300.pd

Uranus deep atmosphere revealed

We have examined Uranus' radio spectrum and the latitudinal variation in its radio brightness temperature. We conclude that Uranus' spectrum cannot be explained with models based upon thermochemical equilibrium. The spectrum can only be matched if there is a low ammonia volume mixing ratio (a few times 10-7 between roughly 150 T 3 at deep tropospheric levels through the formation of an NH4SH-solid cloud, a large concentration of H2S gas is needed, which implies that sulfur must be enhanced relative to its solar value by a factor of a few hundred. We further can constrain the S/N ratio to be at least five times the solar value if the H2O volume mixing ratio is 2O is [greater, approximate]500 x solar. The elemental ratios derived from our work support the theory of planetary accretion by Pollack and Bodenheimer (1989, in Origin and Evolution of Atmospheres (S. Atreya, Ed.)). The constant mixing ratio of NH3 in the 145 T [less, approximate] 240 K range implies either vertical mixing on time scales much shorter than expected from reasonable values of the eddy diffusion coefficient, or supersaturation of NH4SH at levels where T [less, approximate] 240 K. The pole-equator gradient in Uranus' radio brightness temperature implies a latitude-dependent variation in the ammonia mixing ratio, ranging from a few times 10-6 at altitudes where the temperature is 145 T -7 at 145 T -7 down to 280 K in the polar region. Whereas the values in the equator and midlatitudes can be explained by condensation theories, the polar value can only be explained by invoking strong downdrafts of dry air (gas from which most of the ammonia vapor has been removed by condensation). A comparison of images taken at 2- and 6-cm wavelength show a difference in the spatial distribution of the brightness temperature. In addition, images at both wavelengths show time variability in the zonal brightness distribution.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/27655/1/0000036.pd

Upper limits for undetected trace species in the stratosphere of Titan

In this paper we describe a first quantitative search for several molecules in Titan's stratosphere in Cassini CIRS infrared spectra. These are: ammonia (NH3), methanol (CH3OH), formaldehyde (H2CO), and acetonitrile (CH3CN), all of which are predicted by photochemical models but only the last of which observed, and not in the infrared. We find non-detections in all cases, but derive upper limits on the abundances from low-noise observations at 25{\deg}S and 75{\deg}N. Comparing these constraints to model predictions, we conclude that CIRS is highly unlikely to see NH3 or CH3OH emissions. However, CH3CN and H2CO are closer to CIRS detectability, and we suggest ways in which the sensitivity threshold may be lowered towards this goal.Comment: 11 pages plus 6 figure file

New Insights in the Contribution of Voltage-Gated Nav Channels to Rat Aorta Contraction

BACKGROUND: Despite increasing evidence for the presence of voltage-gated Na(+) channels (Na(v)) isoforms and measurements of Na(v) channel currents with the patch-clamp technique in arterial myocytes, no information is available to date as to whether or not Na(v) channels play a functional role in arteries. The aim of the present work was to look for a physiological role of Na(v) channels in the control of rat aortic contraction. METHODOLOGY/PRINCIPAL FINDINGS: Na(v) channels were detected in the aortic media by Western blot analysis and double immunofluorescence labeling for Na(v) channels and smooth muscle alpha-actin using specific antibodies. In parallel, using real time RT-PCR, we identified three Na(v) transcripts: Na(v)1.2, Na(v)1.3, and Na(v)1.5. Only the Na(v)1.2 isoform was found in the intact media and in freshly isolated myocytes excluding contamination by other cell types. Using the specific Na(v) channel agonist veratridine and antagonist tetrodotoxin (TTX), we unmasked a contribution of these channels in the response to the depolarizing agent KCl on rat aortic isometric tension recorded from endothelium-denuded aortic rings. Experimental conditions excluded a contribution of Na(v) channels from the perivascular sympathetic nerve terminals. Addition of low concentrations of KCl (2-10 mM), which induced moderate membrane depolarization (e.g., from -55.9+/-1.4 mV to -45.9+/-1.2 mV at 10 mmol/L as measured with microelectrodes), triggered a contraction potentiated by veratridine (100 microM) and blocked by TTX (1 microM). KB-R7943, an inhibitor of the reverse mode of the Na(+)/Ca(2+) exchanger, mimicked the effect of TTX and had no additive effect in presence of TTX. CONCLUSIONS/SIGNIFICANCE: These results define a new role for Na(v) channels in arterial physiology, and suggest that the TTX-sensitive Na(v)1.2 isoform, together with the Na(+)/Ca(2+) exchanger, contributes to the contractile response of aortic myocytes at physiological range of membrane depolarization

Improved Innate and Adaptive Immunostimulation by Genetically Modified HIV-1 Protein Expressing NYVAC Vectors.

Attenuated poxviruses are safe and capable of expressing foreign antigens. Poxviruses are applied in veterinary vaccination and explored as candidate vaccines for humans. However, poxviruses express multiple genes encoding proteins that interfere with components of the innate and adaptive immune response. This manuscript describes two strategies aimed to improve the immunogenicity of the highly attenuated, host-range restricted poxvirus NYVAC: deletion of the viral gene encoding type-I interferon-binding protein and development of attenuated replication-competent NYVAC. We evaluated these newly generated NYVAC mutants, encoding HIV-1 env, gag, pol and nef, for their ability to stimulate HIV-specific CD8 T-cell responses in vitro from blood mononuclear cells of HIV-infected subjects. The new vectors were evaluated and compared to the parental NYVAC vector in dendritic cells (DCs), RNA expression arrays, HIV gag expression and cross-presentation assays in vitro. Deletion of type-I interferon-binding protein enhanced expression of interferon and interferon-induced genes in DCs, and increased maturation of infected DCs. Restoration of replication competence induced activation of pathways involving antigen processing and presentation. Also, replication-competent NYVAC showed increased Gag expression in infected cells, permitting enhanced cross-presentation to HIV-specific CD8 T cells and proliferation of HIV-specific memory CD8 T-cells in vitro. The recombinant NYVAC combining both modifications induced interferon-induced genes and genes involved in antigen processing and presentation, as well as increased Gag expression. This combined replication-competent NYVAC is a promising candidate for the next generation of HIV vaccines